This study investigates the impact of 24/7 library operations on the health and work-life balance of Library and Information Science (LIS) professionals in India. Using a mixed-method approach with 52 respondents, reveals significant physical and mental health issues, sleep disruptions, and dietary challenges. Qualitative insights highlight personal and professional hurdles, gender disparities, and concerns about workload and safety. The findings underscore the need for tailored support and infrastructure improvements in continuous library settings. The study contributes to understanding the multifaceted challenges faced by LIS professionals and provides recommendations to enhance overall well-being in 24/7 operational environments.