Index Blood Research Articles

Application of a novel extracorporeal membrane oxygenation system in awake Hu sheep under various durations

BackgroundExtracorporeal membrane oxygenation (ECMO) provides effective support for respiratory and circulatory functions and stands as an essential means in the management of life-threatening conditions. This study aimed to evaluate the safety and efficacy of a novel ECMO system in awake sheep models across various support durations.MethodsTen healthy Hu sheep were divided into venovenous (VV) and venoarterial (VA) groups, further categorized into short, medium, and long-term observation subgroups. Vital signs and blood indices were meticulously monitored. The integrity of heparin coatings and thrombosis in the ECMO system were assessed at indicated time points, followed by histopathological analysis.ResultsAll ten sheep survived to their planned endpoints under awake ECMO, exhibiting stable vital signs without notable reductions in blood cells. With the support of ECMO, all sheep demonstrated a significant increase in partial pressure of oxygen (PO2) and maintained oxygen saturation (SO2) between 99.8 and 100%. The heparin coating remained relatively intact even after prolonged ECMO operation. Anatomical observations and pathological histology analyses of major organs revealed no apparent abnormalities.ConclusionsIn healthy Hu sheep models with different maintenance periods, the novel ECMO support in awake state demonstrated high safety and feasibility.

Association of Perinatal Factors and Family Sociodemographic Characteristics With Cardiometabolic Health in Pre-Adolescence: Cross-Sectional Results From Healthy Growth Study.

Study the influence of a variety of perinatal factors on the presence of metabolic syndrome and its parameters in prepubertal age, considering socio-demographic factors, sex, Tanner stage and weight status of the preadolescent. Data collected from the cross-sectional Healthy Growth Study (2007) in students from four prefectures of Greece and their families. Cardiometabolic risk factors (waist circumference, blood pressure and blood indices) and perinatal data (from parents and health records) were collected. Single and a multiple logistic regression performed to assess the associations with cardiometabolic risk in preadolescence. Dependent variables included waist circumference, blood glucose, HDL-C, TG, blood pressure and metabolic syndrome presence, while independent variables encompassed perinatal factors categorised as pre-pregnancy, during and post-pregnancy. 2666 pre-adolescents (mean age 11.2 year) participated in the study. After adjustment for parental educational level, family income, sex, Tanner stage and weight category, maternal pre-pregnancy weight was positively associated with high waist circumference (OR, 95% CI 1.02 (1.00-1.04)), low HDL-C levels (OR, 95% CI 1.01 (1.00-1.03)) and metabolic syndrome (OR, 95% CI 1.02 (1.00-1.05)). Timing of solid foods' introduction was positively associated with high waist circumference (OR, 95% CI 1.09 (1.01-1.08)), gestational age with high glucose levels (OR, 95% CI 1.12 (1.04-1.19)), smoking (OR, 95% CI 1.07 (1.00-1.14)) and alcohol consumption (OR, 95% CI 2.35 (1.05-5.27)) during the first trimester of breastfeeding with high glucose levels and low HDL-C levels, respectively. Number of previous miscarriages was positively associated with low HDL-C levels (OR, 95% CI 1.28 (1.04-1.57)). Gestational age was negatively associated with high waist circumference (OR, 95% CI 0.87 (0.80-0.95)) and iron supplementation in the third pregnancy trimester negatively association with high TG levels (OR, 95% CI 0.34 (0.12-0.91)). Maternal weight, perinatal behaviours, infant feeding and miscarriages influence pre-pubertal cardiometabolic health, independent of sociodemographics, sex, Tanner stage and weight.

Effect of supplementation of phytoadditive mixtures on live weight, blood indices, hormonal profile and reproductive performance in Black Bengal goats

The study was conducted to evaluate the effect of phytoadditive mixtures containing equal proportions of Curcuma longa & Allium sativum (PAM-1) and Curcuma longa & Ocimum sanctum (PAM-2) on live weight, blood parameters, hormonal profile and reproductive performance in Black Bengal goats. A total of 21 post weaned female kids of 3–4 months age and similar body weights maintained at Goat Research Unit of Eastern Regional Station (ERS), National Dairy Research Institute (NDRI), Kalyani, India during the year 2021–2022 were randomly distributed in three groups namely, Control, PAM-1 and PAM-2 for 10 months. Blood parameters i.e. glucose, urea, total protein, albumin, globulin, ALT, AST, ALP, total lipids, cholesterol, and Triglycerides, WBC, RBC, Hb and PCV %; NEFA levels and hormones i.e. prolactin, cortisol, T3 and T4 were estimated in all three experimental groups. Higher (P < 0.001) live weights were investigated in supplemented goats. Blood parameters including glucose, urea, total protein, albumin, globulin, ALT, AST, ALP, WBC, RBC, Hb and PCV% were similar (P > 0.05) in all groups. Lower (P < 0.001) blood lipids were found in supplemented groups. Higher Prolactin, T3 and T4 levels and lower Cortisol levels were reported in PAM-1 and PAM-2 groups (P < 0.001) than control group. Age at first estrus, first conception, first kidding and post kidding first estrus days were lower (P < 0.001) in supplemented groups. Therefore, it was concluded that supplementation of PAM-1 and PAM-2 improved reproductive performance, hormonal profile and lipid profile through improved live weights in Black Bengal goats.

Optimizing broiler performance, carcass traits, and health: evaluating thyme and/or garlic powders as natural growth promoters in antibiotic-free diets.

The purpose of this study was to evaluate the effects of thyme powder (TP), garlic powder (GP), and their combination as natural alternatives to antibiotics on broiler growth performance, blood indices, and carcass characteristics. A completely randomized design was used, with four experimental groups consisting of 264 one-day-old Ross 308 broiler chicks (45.27±0.5 g). Each group received different levels of TP and GP in the diet as follows: a control group (basal diet with no additives), 2 g of GP/kg, 2 g of TP/kg, and a mixture treatment (1 g of GP/kg + 1 g of TP/kg). Each treatment included six replicates, with eleven unsexed chicks per replicate. The results showed that supplementation with TP, GP, and their combination significantly improved feed intake (FI), feed conversion ratio (FCR), and overall growth performance compared to the untreated group (UG). The different additives also significantly (P≤0.05) enhanced carcass characteristics, particularly carcass yield and heart weight, both in absolute and relative terms. Furthermore, the treatments significantly (P≤0.05) affected blood indices, leading to notable improvements in immune and antioxidant parameters, including increased levels of immunoglobulin Y (IgY), immunoglobulin M (IgM), and superoxide dismutase (SOD), as well as decreased levels of malondialdehyde (MDA), a marker of oxidative stress. These changes suggest that the supplemented groups had enhanced immunity and a better oxidative balance, contributing to improved overall health. Notably, the combined treatment of TP and GP (1% each) exhibited a synergistic effect, yielding benefits comparable to or even surpassing those of the individual TP or GP treatments. These findings support the potential of combining these natural additives to achieve a balanced improvement in growth, feed efficiency, and health status. In conclusion, supplementation with thyme powder, garlic powder, or a combination of both at appropriate levels (2% GP, 2% TP, or 1% each) can serve as an effective alternative to antibiotics, improving broiler growth performance, carcass quality, and health by enhancing immune responses and antioxidant status.

Effect of different intensity aerobic exercise on remodeling immune microenvironment of adipose tissue in obesity mouse.

Obesity can change the immune microenvironment of adipose tissue and induce inflammation. This study is dedicated to exploring the internal mechanism by which different intensities of exercise reprogram the immune microenvironment of epididymal adipose tissue in nutritionally obese mice. C57BL/6J male obese mouse models were constructed by high-fat diet, which were respectively obese control group (OC), moderate-intensity continuous exercise group (HF-M), high-intensity continuous exercise group (HF-H), and high-intensity intermittent exercise group (HF-T). The exercise group was subjected to aerobic exercise intervention for 8 wk, and samples of mice were collected at the fourth and eighth week, respectively. Mice blood, liver, and adipose tissue of the epididymis were collected for index detection and adipose tissue ordinary transcriptome sequencing. After exercise intervention, when compared with the OC group, the morphology and blood indexes of the exercise groups were significantly improved. The liver lipid content was decreased, adipose tissue inflammation was reduced, and the mRNA and protein expression levels of IL-1β, F4/80, and CD64 in adipose tissue were significantly decreased (P < 0.01). Among the three exercise groups, the effect of the HF-T group was more significant. When compared with the OC group, fibroblast-specific marker genes, neutrophil marker genes, macrophage marker genes, and immune-related signaling pathways were significantly downregulated in the HF-T group. Exercise can reshape the immune microenvironment of adipose tissue, and high-intensity intermittent aerobic exercise is the most effective.NEW & NOTEWORTHY The present study has revealed that obesity is capable of altering the immune microenvironment within adipose tissue, thereby giving rise to inflammation. It has been demonstrated that exercise holds the potential to reverse the onset of inflammatory responses, with high-intensity intermittent aerobic exercise emerging as the most efficacious approach.

Experimental study of the combined effect of benzene exposure and physical load in rats

Introduction. The working process in many industries is often associated not only with chemical exposures but also with heavy physical workload, which can be potent of toxic effects of the former differently. The purpose of the study was to establish body responses to effects of benzene exposure combined with exercise in an experiment on rats. Materials and methods. The study was conducted on mature male Wistar rats, ten animals in each of the following groups: “Control”, “Running”, “Benzene exposure”, and “Benzene exposure + running”. Subchronic toxicity was modelled by intragastric administration of benzene thrice a week for 4 weeks in a cumulative dose of 100 mg/kg body weight. Exercise was modelled by means of 10-minute forced running sessions at a speed of 25 m/min 5 times a week for 4 weeks. We then measured post-exposure biochemical and hematological indices and the proportion of different types of cells in imprint smears of rat internal organs. Statistical data analysis was carried out using the Student’s t-test with p&lt;0.05. For mathematical modelling of the combined effect of factors, we applied a Response Surface Method (RSM) with the construction of Loewe isobols on its basis. Results. Almost no statistically significant changes were registered in the rodents who exercised while statistical ones were sporadic and diagnostically insignificant in most cases. Benzene exposure induced changes in blood indices indicating blood clotting disorders, typical of chronic exposure to benzene, and in the antioxidant system. The impact of running on health effects of benzene exposure was ambiguous but isobolographic analysis showed possible additivity and even synergy, both detrimental to health, in 15.6 % of cases. Limitations. Extrapolation of the study results to human should be done with caution, since the combined effect has been examined only for subchronic exposure using a single species and sex of laboratory animals. Conclusion. Additivity proven by a number of indices of toxic effect gives important information for understanding and predicting health effects of exposure to organic pollutants combined with physical activity.

Correlation Red Blood Inidces to Hemogloblin Levels In Addolescent At SMK-TI High School, Badung, Bali

The Complete Blood Count (CBC) or hemogram is a panel of test results on the cellular components of blood: the Red Blood Cells (RBCS), White Blood Cells (WBCS), and platelets (PLTS). Each of the portions can be interpreted separately in a methodical fashion and the interpretation has two aims for laboratorians. parameter that is quite sensitive to testing errors and interferences (often flagged) is the Mean Cell Hemoglobin Concentration (MCHC). The purpose of checking Hemoglobin levels is to assess the level of anemia, response to anemia therapy, or the development of diseases related to anemia and polycythemia. Other uses for checking Hemoglobin include assessing the level of anemia, the response to anemic women and the development of diseases related to anemia. This research used a crosssectional analytic method with the aim of finding a correlation red blood indices to Hemoglobin. Teenagers are the most prominent investors and the next generation of the nation's ideals. However, in adolescence, many problems can have a negative impact on health, such as adolescent nutrition. The quality and quantity of nutritional intake consumed during adolescence is an important factor in the emergence of nutritional problems in adolescents.

Study on Hematological and Biochemical Index of Blood and Vitreous Humor in the Celestial-Eye Goldfish

In this study, we conducted an exploration of blood collection, hematological and biochemical comparative analysis of the unique vitreous humor and blood of the celestial-eye goldfish (CE) (Carassius auratus), and obtained hematological and biochemical indicators for healthy celestial goldfish blood. After exploring the optimal method for collecting blood and ocular fluid, blood and vitreous humor samples were drawn from healthy CE, and an automatic hematology analyzer was used to measure 17 physiological indicators in the blood and vitreous humor. Additionally, 20 biochemical indices were measured by an automatic biochemical analyzer. Experiments were also conductedto evaluate the effect of blood and vitreous humor collection on the subsequent survival rate of the CE. The results showed that the blood of CE exhibited values for 17 hematological indicators, including red blood cells (mean value: 2.19 × 1012/L), white blood cells (mean value: 62.21 × 109/L), hemoglobin (mean value:138.25 g/L), and no eosinophils or basophils. We also found that the levels of aspartate amino transferase (AST) and glutamyl transpeptidase (GGT) in the vitreous humor of CE were significantly higher than those in the serum (p &lt; 0.01). No cells were detected in the vitreous humor of CE, and there was no significant difference in survival rate compared to normal goldfish after extracting blood and vitreous humor from CE (p &gt; 0.05). This study provides fundamental data for the cultivation of healthy goldfish, the development of goldfish hematology, and further research on the development and formation of CE’s unique eye traits.

Potential Correlation Between Hematological Parameters and Palpitation in Outpatients With COVID-19: A Retrospective Study.

Research on heart injury caused by COVID-19 is limited to large observational and retrospective cohort studies using imaging or pathological data. Reported changes in the levels of myocardial markers in severe diseases have been limited, with few studies on mild infections. The effects of COVID-19 on cardiac function and changes in myocardial marker levels have not yet been reported. We analyzed data from outpatient blood samples collected at Beijing Anzhen Hospital during the 2022 COVID-19 outbreak and used the same periods in 2020 and 2021 as controls, focusing on changes in routine blood tests, coagulation, myocardial markers, and other blood indices in patients with palpitations. The number of patients with palpitations increased by 4.87-fold during the COVID-19 mass outbreak in 2022. The indices of myocardial damage did not show any symptom-related increases but decreased within the normal range. The proportion of patients with palpitations whose D-dimer and fibrinogen/fibrin degradation product (FDP) values exceeded the reference ranges increased, as did the numbers of neutrophils, monocytes, and platelets. In this retrospective analysis, we found little change in the myocardial markers in patients with mild COVID-19. In patients with mild COVID-19, attention should be diverted from detecting myocardial markers to changes in coagulation test results, focusing on the levels of coagulation indices to improve circulation and prevent thrombosis.

Influence of Multistrain Probiotics on Growth, Hematology, Gut and Liver Morphometry, and GH and IGFs Genes Expression in Rohu (Labeo Rohita) Fry

Probiotic is regarded as an effective feed additive to increase the growth, feeding efficiency, survivability, and overall productivity of fishes in aquaculture. The following study was intended to examine the effects of multistrain probiotics on growth, blood indices, histomorphometry of gastrointestinal tract and liver, and growth hormone (GH) and insulin‐like growth factors (IGFs) genes expression in rohu Labeo rohita. Probiotic containing Bacillus subtilis, B. thuringiencis, Lactobacillus plantarum, and L. buchneri was inoculated in water and three different doses, that is, 0 mL/L (control), 0.5 mL/L (lower dose, T1), and 1.0 mL/L (higher dose, T2) were used in this study. Each treatment had three replicates and fishes were reared in aquarium for 60 days. Results revealed that substantially higher weight gain (WG) and specific growth rate (SGR) along with least apparent feed conversion ratio (AFC) were documented in probiotic treated fish which was significantly different than that of control, however, higher dose (1.0 mL/L) showed best performance. Blood–biochemical indices such as red blood cell (RBC), white blood cell (WBC), hemoglobin (Hb), and glucose (Glu) level were observed statistically higher in probiotic additive culture water. Moreover, the inoculation of multispecies probiotic positively altered the intestinal histometry. Besides, immune response features of intestine were observed significantly higher in probiotic treated fish. The nucleus shape and distances between liver tissues (DLTs) were also improved with the upward doses of probiotic application. The mRNAs levels of GH in the pituitary and IGF‐1 and IGF‐2 in the liver were significantly higher in probiotic treated fish. In a nutshell, this study suggested that the inclusion of multistrain probiotics showed promising improvement in growth performance and feed conversion efficiency; additionally, uplifted the intestinal structure integrity, liver morphology, and GH and IGFs genes expression in rohu.

Predictive Value of the Triglyceride-Glucose Index for Metabolic-Associated Fatty Liver Disease in Individuals with Different Metabolic Obese Phenotypes.

To investigate the relationship between triglyceride-glucose (TyG) index and metabolic-associated fatty liver disease (MAFLD), and to evaluate the predictive value of the TyG index for MAFLD in individuals with different metabolic obese phenotypes. The aim is to provide a novel approach for the screening and early diagnosis of MAFLD in the general population. A total of 2614 subjects were recruited and classified into four categories of metabolic obese phenotypes based on their body mass index (BMI) and metabolic status. Basic data and general blood indices were collected and analyzed. The TyG index was calculated, and an abdominal ultrasound was performed to detect the presence of fatty liver disease. The aforementioned data were then subjected to statistical analysis. The TyG index was significantly higher in the MAFLD group than in the non-MAFLD group (P < 0.001). Furthermore, the TyG index in the metabolically unhealthy and obese (MUO) group and the metabolically unhealthy normal weight (MUNW) group was significantly higher than that in the metabolically healthy and obese (MHO) group and the metabolically healthy normal weight (MHNW) group (P < 0.001). The area under the curve (AUC) of the TyG index for predicting MAFLD in the MHNW, MUNW, MHO, and MUO groups was 0.765, 0.766, 0.659, and 0.650, respectively. The critical values were 8.575, 9.075, 8.795, and 9.165, respectively. The TyG index is a reliable predictor of MAFLD risk, exhibiting a higher predictive ability for the risk of developing MAFLD in individuals with normal BMI compared to those with abnormal BMI. The findings of this study lend support for the use of the TyG index as a screening tool and for guiding subsequent management of patients with MAFLD.

Role of the Systemic Immune-Inflammation Index and Systemic Immune-Response Index in the Prediction of Adverse Outcomes in Pregnant Women With Antiphospholipid Syndrome.

This study aims to evaluate the role of the systemic immune-inflammation index (SII) and the systemic immune-response index (SIRI) in predicting adverse perinatal outcomes (APO) in pregnant women with antiphospholipid syndrome (APS). This is a retrospective case-control study at the tertiary center, between January 2015 and January 2023. The study included APS cases and a low-risk control group. Pregnant women with APS (n=52) and controls (n=104) were compared between SII and SIRI values taken in the first trimester (1) and the last month before birth (2). It was examined whether these indexes predicted APO in cases with APS. In the APS group, SII and SIRI values taken in the first trimester (1) and in the last month before birth (2) were significantly lower than in the control group (p=0.015, p=0.023, p=0.001, p=0.001, respectively). The small for gestational age (SGA) rate was 30.8% and the stillbirth rate was 11.5% in the APS group (p=0.017, p=0.001). The optimum cutoff values for SGA were 584.97 (75% sensitivity, 77.8% specificity), 688.50 (62.5% sensitivity, 62.9% specificity), and 1.02 (56.3% sensitivity, 77.8% specificity) for SII 1, SII 2, and SIRI 1, respectively. The optimum cutoff value for stillbirth was 1.23 for SIRI 2 (83.3% sensitivity, 89.1% specificity, p=0.004). Pregnant women with APS had decreased blood indices in the first trimester and the last month before birth compared to the control group. In cases with APS, these indices can predict APOs like SGA and stillbirth.

Exploring the Association Between Blood Indices and Skin and Joint Activity of Psoriatic Arthritis

The exact cause of psoriatic arthritis is still unknown, but hypotheses suggest the role of hematological parameters in the onset and severity of the disease. This study evaluated the hematological indices and their association with skin and joint activity in psoriatic arthritis. This cross-sectional study included 74 patients with psoriatic arthritis. Demographical and clinical data, blood indices, psoriasis area and severity index (PASI) score and disease activity in psoriatic arthritis (DAPSA) scores were calculated for all patients. The mean age of the patients was 48.89±12.03 years and most were female (n=49). A significant correlation was observed between age and number of underlying diseases with PASI and DAPSA scores. Mean PASI and DAPSA scores were 5.19 and 15.13, respectively. The severity of psoriasis was mild in 58.1%, moderate in 36.5%, and severe in 4.5% of the cases. The activity of psoriatic arthritis was improved in 2.1%, low in 55.4%, moderate in 24.3%, and high in 1.8% of the patients. A significant association was found between erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), red cell distribution width (RDW), neutrophil-to-lymphocyte ratio (NLR), platelet (PLT) count, mean platelet volume (MPV), and PASI scores, while no statistically significant association was reported for PLR. A significant correlation was observed between ESR, CRP, RDW, NLR, PLR, PLT, and DAPSA scores, while no statistically significant association was found for MPV. The findings indicated that inflammatory and hematological markers can be helpful factors in evaluating the severity of psoriasis and psoriatic arthritis.

Systemic immune-inflammatory index predict short-term outcome in recurrent/metastatic and locally advanced cervical cancer patients treated with PD-1 inhibitor

This study aims to assess the predictive value of certain markers of inflammation in patients with locally advanced or recurrent/metastatic cervical cancer who are undergoing treatment with anti-programmed death 1 (PD-1) therapy. A total of 105 patients with cervical cancer, who received treatment involving immunocheckpoint inhibitors (ICIs), were included in this retrospective study. We collected information on various peripheral blood indices, including the neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), lymphocyte-to-monocyte ratio (LMR), systemic immune-inflammation index (SII), and prognostic nutritional index (PNI). To determine the appropriate cutoff values for these inflammatory markers, we performed receiver operating characteristic curve (ROC) analysis. Progression-free survival (PFS) was estimated using the Kaplan-Meier method, and we conducted both univariate and multivariate Cox regression analyses to evaluate the prognostic value of these markers. Out of the 105 patients who received ICI treatment, the median progression-free survival (mPFS) was 19.0 months. We obtained the patients’ clinical characteristics, such as age, pathological type, therapy regimen, Figo stage, NLR, PLR, LMR, SII, and PNI from their medical records. The optimal cutoff values for NLR, PLR, LMR, SII, and PNI were determined as 3.76, 218.1, 3.34, 1147.7, 43.75, respectively. In the univariate analysis, age, pathological type, therapy regimen, Figo stage, and LMR were not found to be associated with PFS. However, high NLR(P=0.001), high PLR(P<0.001), high SII(P<0.001), and low PNI (P=0.003)were all associated with shorter PFS. Multivariate analysis indicated that SII (P=0.017) was an independent risk factor for PFS. This study highlights the potential use of SII as a predictor of progression-free survival in cervical cancer patients undergoing immunotherapy.

INDICES OF SYSTEMIC INFLAMMATION IN VERY LOW BIRTH WEIGHT PRETERM INFANTS WITH SEVERE PERINATAL PATHOLOGY

Hematopoiesis plays a pivotal role in providing compensatory and adaptive mechanisms of the body in response to stressors associated with birth and in facilitating adaptation to new conditions of existence. The evaluation of the complete blood count in newborns is a recommended laboratory test for perinatal pathology, as outlined in standard practice guidelines. The interpretation of results from peripheral blood analysis, with consideration of ratios of indicators and the calculation of systemic inflammation indices, enables not only the assessment of the hematopoietic system but also the determination of specific features of the child's adaptation in the context of preterm birth. Materials and methods. A comprehensive clinical and laboratory examination of the newborns was conducted following standard protocols. Based on the results of the complete blood count, additional ratios were calculated, including the neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), and monocyte-to-lymphocyte ratio (MLR). Additionally, the following indices were calculated: systemic immune inflammation index (SII), systemic inflammation response index (SIRI), and platelet, neutrophil, monocyte, and lymphocyte index (PIV). Statistical analysis was performed using STATISTICA software (StatSoft Inc., USA, Version 10). Quantitative indicators with a normal distribution were compared using Student's t-test, and differences were considered statistically significant at p &lt; 0.0001. Results. The severity of the newborns’ condition is accompanied by characteristic changes in complete blood count parameters, including ratios and indices. The results demonstrated specific alterations that suggest the presence of distinct deviations in the indicators when compared to the control group. This indicates a disruption in the activation of erythroid, myeloid, and granulocyte germ cells of hematopoiesis in response to hypoxia during the early neonatal period in extremely preterm infants. The utilization of these indicators provides a degree of insight into the pathophysiological alterations occurring in the children under hypoxic inflammation during preterm birth. Conclusion. Determining the ratio of traditional CBC indicators and systemic inflammation indices is a valuable addition to the traditional assessment of laboratory results in perinatal pathology for preterm infants. Calculating the ratio of complete blood count and systemic inflammation indices in newborns provides an additional criterion for assessing the severity of the condition, helping to predict the progression of perinatal pathology, with consideration of gestational age. An in-depth analysis of complete blood count results facilitates a more objective evaluation of the pathophysiological mechanisms of hypoxic inflammation and their specific characteristics in preterm infants.

Prevalence and Complications of Anemia Across Pregnancy Trimesters: A Cohort Study

Background: Anemia is a common condition affecting approximately 40% of pregnant women, posing serious risks to maternal and fetal health. These risks include premature birth, low birth weight, infections, postpartum hemorrhage, and increased likelihood of cesarean section. Materials and Methods: This retrospective cohort study analyzed data from 390 pregnant women who attended the gynecology and obstetrics clinic at Amir Al-Momenin Hospital. Participants were selected based on referrals on a predetermined date. Data collection and analysis were conducted using STATA version 11. Pregnancy outcomes between anemic and non-anemic women were compared using independent t-tests, chi-square tests, and multivariate logistic regression models. Results: The study found no significant association between low hemoglobin and hematocrit levels in the first trimester and pregnancy outcomes. However, low hematocrit levels in the second trimester were significantly associated with increased risks of stillbirth, low Apgar scores, and premature delivery. No significant effects were observed on other maternal or neonatal outcomes. Conclusion: Hematocrit levels in the second trimester may serve as a critical predictor of adverse pregnancy outcomes for both mother and infant. Screening for anemia and monitoring blood indices before and during pregnancy are essential. Timely diagnosis and appropriate treatment of anemia can help mitigate risks to maternal and fetal health.

Clinical and immunological characteristics of full-term newborns from mothers with COVID-19 during pregnancy

The long-term consequences of COVID-19 still remain among priority issues. Special attention should be paid to the state of health of newborns whose mothers suffered a coronavirus infection during pregnancy. The aim of our study was to evaluate clinical and immunological parameters in full-term newborns from mothers who underwent COVID-19 during pregnancy. We have performed a prospective cohort comparative study. A group of full-term newborns from mothers who had mild or moderate COVID-19 at different stages of pregnancy (n = 134) was compared with a group of newborns from mothers who were not exposed to SARS-CoV-2 infection during their pregnancy (n = 84). Individual health indices and immunological characteristics were also evaluated at the age of 3 months of infants’ life. For statistical analysis, the Microsoft Excel computer software package (2010), SPSS Statistics version 22.0 (IBM Microsoft, USA) was used. Statistical hypotheses about the absence of intergroup differences in quantitative characteristics were tested using the Mann–Whitney U criterion, the Student’s criterion, and binary characteristics were assessed with Chi-square test (÷2). Qualitative data are presented as an absolute value (n) and percentages. The results were considered reliable at a significance level of p0.05. It was found that, in the neonatal period, there were no statistically significant differences in gender characteristics, anthropometric characteristics and Apgar scores between the groups. There were no differences in the incidence rate in the neonatal period. At the age of 3 months of life, there was a higher incidence of somatic and neurological morbidity in children whose mothers had suffered a new coronavirus infection during pregnancy (OR = 3.78 (1.5; 9.51); p0.001). It was found that the levels of hematocrit (p = 0.001), lymphocytes (p0.001), platelets (p0.001) were statistically significantly lower in newborns in group I. When analyzing the levels of umbilical cord blood cytokines in the two groups, it was found that the levels of TNF, IL-6, VEGF-A, IL-10, IL-1á, IL-1â, IL-8 (p ≤ 0.001) were higher in group I compared with group II. The levels of cytokines in their mothers showed a positive correlation with appropriate indexes in umbilical cord blood. Strong positive associations were found in the mother-child pairs on the 3rd trimester of pregnancy, in terms of TNFá, IL-1â, moderate positive correlations were revealed for IL-8, IL-10. Hence, despite the absence of clinical manifestations of infectious process and other disorders of early neonatal adaptation, the laboratory changes in general blood counts and active expression of proinflammatory cytokines in newborns whose mothers suffered COVID-19 during pregnancy may suggest the presence of a fetal systemic inflammatory response. An increased morbidity at the age of 3 months may be the result of low-level inflammation.

The establishment of PD-1 inhibitor treatment prognosis model based on dynamic changes of peripheral blood indexes in patients with advanced lung squamous cell carcinoma.

Unlike patients with lung adenocarcinoma, patients with lung squamous cell carcinoma (LUSC) do not derive significant benefits from targeted therapy. In recent years, immunotherapy has revolutionized the treatment approach for LUSC. However, not all patients with this type of cancer respond to immunotherapy, necessitating the identification of effective biomarkers to predict survival prognosis and evaluate the efficacy of PD-1 inhibitors. We retrospectively collected case and hematologic data from 212 patients with advanced squamous lung cancer who received PD-1 combination therapy. Hematological indices mainly contained SCC, CEA, NSE, Hb, LDH, WBC and RBC at baseline, 6 and 12 weeks of treatment. All patients underwent imaging examinations and efficacy was evaluated according to RECIST1.1 criteria. Univariate tests were used to assess the relationship between changes in serum biomarkers, clinical characteristics and treatment outcome. The survival prognosis of patients was investigated by telephone follow-up. The optimal critical values of all hematological indicators were calculated by ROC curves, and then logistic regression and Cox regression were used to analyze multiple serum markers in relation to efficacy and survival prognosis, respectively. Finally, column line plots were constructed and validated to predict the probability of patient survival. Post-treatment RBC12w<3.81 × 10 12/L (p < 0.034) was associated with lower ORR, and WBC6w<9.34 × 109/L (p=0.041) was associated with higher DCR.SCC12w≥2.25 ng/mL (p = 0.015), NSE6w≥13.54 ng/mL(p = 0.044)and RBC0w≥4.2 × 10 9/L (p = 0.003) were independent predictors of PFS. SCC12w≥2.25 ng/mL (p < 0.001) and NSE6w≥13.54ng/mL(p = 0.042) were independent predictor of OS. Patients in the SCC12w≥2.25 ng/mL (HR = 1.943,95% CI:1.218-3.079 vs. HR = 2.161,95%CI:1.087-3.241) and NSE6w≥13.54 ng/mL (HR = 1.657,95% CI:1.118-2.535 vs. HR = 2.064,95% CI:1.569-4.169) groups had shorter PFS and OS. In subgroup analysis, patients with stage III advanced squamous lung cancer had a better pro-gnosis than those with stage IV. PD-L1-positive, and SCC12w ≥2.25 ng/mL had a worse prognosis. The results of constructing column-line plots for predicting the survival probability of 1-, 3-, and 5-year PFS and OS: The C-index and 95% CI for PFS and OS of column-line plots were 0.725 (95% CI: 0.478-1.928) and 0.755 (95% CI: 0.642-0.868), respectively, and the bootstrap correction showed a good consistency of the column-line plots. Changes in RBC12w ≥3.81×1012/L, WBC6w ≥9.34×10 9/L, SCC12w ≥2.25 ng/mL, and NSE6w ≥13.54 ng/mL after treatment are prognostic indicators of immunotherapy in patients with advanced squamous lung cancer.

Digital Imaging of Peripheral Blood Smear With MC-80 as a Screening Tool for Thalassemia.

Thalassemia, a genetic blood disorder, poses significant global health challenges, emphasizing the importance of accurate screening methods. Traditional diagnostic tools, such as osmotic fragility and dichlorophenolindophenol tests, along with blood indices, such as mean corpuscular volume and mean corpuscular hemoglobin, have limitations. Digital microscopy of peripheral blood smears is a promising alternative for objective quantification and standardization. Blood samples from 81 thalassemia screening-negative and 41 screening-positive individuals were analyzed using Mindray MC-80 Digital Morphology. Pre-classification of red blood cell (RBC) morphology using Mindray MC-80 revealed significant differences between the screening-positive and screening-negative groups. Various RBC morphologies demonstrated statistically significant variance, including hypochromic cells, schistocytes, elliptocytes, target cells, teardrop cells (p < 0.001), and ovalocytes (p = 0.002). However, the area under the receiver operating characteristic curve of these parameters was < 0.8, indicating a limited discriminatory power. RBC morphology showed promise in detecting subtle changes associated with thalassemia. However, it may not be sufficient for accurate screening alone, highlighting the need for complementary diagnostic approaches.

Association between psychiatric symptoms with multiple peripheral blood sample test: a 10-year retrospective study

BackgroundPsychiatric illness is thought to be a brain somatic crosstalk disorder. However, the existing phenomenology-based Diagnostic and Statistical Manual of Mental Disorders, Fifth Edition (DSM-5) diagnostic framework overlooks various dimensions other than symptoms. In this study, we investigated the associations between peripheral blood test indexes with various symptom levels of major depressive disorder (MDD), bipolar disorder (BD), and schizophrenia (SCZ) to explore the availability of peripheral blood test indexes.MethodsWe extracted cases diagnosed with MDD, BD, and SCZ at West China Hospital from 2009 to 2021, translated their main complaints into Research Domain Criteria (RDoC) symptom severity scores using nature language processing (NLP), and collected their detailed psychiatric symptoms and peripheral blood test results. Then, generalized linear models were performed between seven types of peripheral blood test values with their transformed RDoC scores and detailed symptom information adjusted for age, gender, smoking, and alcohol history.ResultsSeveral inflammatory-related indexes were strongly associated with the negative valence system (NVS) domain (basophil percentage adjusted β = 0.275, lymphocyte percentage adjusted β = 0.271, monocyte percentage adjusted β = 0.223, neutrophil percentage adjusted β = −0.310, neutrophil count adjusted β = −0.301, glucose adjusted β = −0.287, leukocyte count adjusted β = −0.244, NLR adjusted β = −0.229, and total protein adjusted β = −0.170), the positive valence system (PVS) domain (monocyte percentage adjusted β = 0.228, basophil count adjusted β = 0.176, and glutamyl transpeptidase adjusted β = 0.171), and a wide range of mood, reward, and psychomotor symptoms. In addition, glucose, urea, urate, cystatin C, and albumin showed considerable associations with multiple symptoms. In addition, based on the direction of associations and the similarity of symptoms in terms of RDoC thinking, it is suggested that “positive” mood symptoms like mania and irritability and “negative” mood symptoms like depression and anxiety might be on a continuum considering their opposite relationships with similar blood indexes.LimitationsThe cross-sectional design, limited symptoms record, and high proportion of missing values in some other peripheral blood indexes limited our findings.ConclusionThe proportion of high inflammatory indexes in SCZ was relatively high, but in terms of mean values, SCZ, BD, and MDD did not differ significantly. Inflammatory response showed a strong correlation with NVS, PVS, and a range of psychiatric symptoms especially mood symptoms, psychomotor symptoms, and cognitive abilities.