Independent Fashion Research Articles

An intrinsic role of IL-33 in Treg cell-mediated tumor immunoevasion.

Regulatory T (Treg) cells accumulate into tumors hindering the success of cancer immunotherapy. Yet, therapeutic targeting of Treg cells shows limited efficacy or leads to autoimmunity. The molecular mechanisms that guide Treg cell stability in tumors, remain elusive. Herein, we identify a cell-intrinsic role of the alarmin IL-33 in the functional stability of Treg cells. Specifically, IL-33-deficient Treg cells demonstrated attenuated suppressive properties in vivo and facilitated tumor regression in an ST2 (IL-33 receptor)-independent fashion. Upon activation, Il33–/– Treg cells exhibited epigenetic reprogramming with increased chromatin accessibility of the Ifng locus leading to elevated interferon-γ (IFN-γ) production in an NF-κB–T-bet-dependent manner. IFN-γ was essential for Treg cell defective function since its ablation restored Il33–/– Treg cell suppressive properties. Importantly, genetic ablation of Il33 potentiated the therapeutic effect of immunotherapy. Our findings reveal a novel and therapeutically important intrinsic role of IL-33 in Treg cell stability in cancer.

Stress-Induced Neuron Remodeling Reveals Differential Interplay Between Neurexin and Environmental Factors in Caenorhabditis elegans.

Neurexins are neuronal adhesion molecules important for synapse maturation, function, and plasticity. Neurexins have been genetically associated with neurodevelopmental disorders, including autism spectrum disorders (ASDs) and schizophrenia, but can have variable penetrance and phenotypic severity. Heritability studies indicate that a significant percentage of risk for ASD and schizophrenia includes environmental factors, highlighting a poorly understood interplay between genetic and environmental factors. The singular Caenorhabditis elegans ortholog of human neurexins, nrx-1, controls experience-dependent morphologic remodeling of a GABAergic neuron in adult males. Here, I show remodeling of this neuron's morphology in response to each of three environmental stressors (nutritional, heat, or genotoxic stress) when applied specifically during sexual maturation. Increased outgrowth of axon-like neurites following adolescent stress is the result of an altered morphologic plasticity in adulthood. Despite remodeling being induced by each of the three stressors, only nutritional stress affects downstream behavior and is dependent on neurexin/nrx-1 Heat or genotoxic stress in adolescence does not alter behavior despite inducing GABAergic neuron remodeling, in a neurexin/nrx-1 independent fashion. Starvation-induced remodeling is also dependent on neuroligin/nlg-1, the canonical binding partner for neurexin/nrx-1, and the transcription factors FOXO/daf-16 and HSF1/hsf-1hsf-1 and daf-16, in addition, each have unique roles in remodeling induced by heat and UV stress. The differential molecular mechanisms underlying GABAergic neuron remodeling in response to different stressors, and the disparate effects of stressors on downstream behavior, are a paradigm for understanding how genetics, environmental exposures, and plasticity may contribute to brain dysfunction in ASDs and schizophrenia.

Post-war fashion tips in the popular fashion press in Poland: A source of psychological support in the 'struggle for femininity'

Abstract Social conditions in Poland were harsh at the conclusion of World War II. Surprisingly, interest in fashion revived quickly after the war, giving rise in 1945 to a hugely popular fashion press, avidly read by Polish women. The independent magazine Fashion and Practical Life (Moda i Życie Praktyczne), launched in December 1945, was the first of its kind in Poland after the war, and quickly gained a large readership of urban and rural women (and significant numbers of men). It centred on fashion tips – from practical advice on how to remodel existing material into new clothing to more aspirational ideas, crossing over from the necessities of dress or clothing into the more imaginary realm of 'fashion'. Unlike readers of the fashion press before the war, the readership of this magazine was not leisured or highly literate, but largely 'ordinary' women trying to deal with the realities of their circumstances and to find relief from them. Letters from readers were the centre of the magazine in its early years. They shared their experiences and solutions. This article explores the phenomenon of a genre of publishing for women in Poland through analysis of Fashion and Practical Life from its inception into the early 1950s. It examines the contexts in which it operated and its role in the representation and self-identity of Polish women within this time of transition. The article identifies two key typologies for fashion tips: 'poor fashion' (how to make available materials into liveable garments) and 'imaginary fashion' (the aspirations that Polish women had but could not attain at that time). It also shows that apparently emancipatory trends for women were short-lived. By the early 1950s the narrative of the fashion press reinscribed Polish women back in the home, as housewives.

Role of Mutant p53 in the Progression of Chronic Lymphocytic Leukemia

Patients with Chronic Lymphocytic Leukemia (CLL) have a variety of chromosomal abnormalities and mutations. At diagnosis, about 10% of CLL patients have deletions of chromosome 17 (Del17p) leading to the loss of one allele of tumor suppressor protein TP53, which increases to over 30% in relapsed/refractory disease. Additionally, 83% of patients with a Del17p acquire a mutation on their second TP53 allele at one of several sites within the DNA binding domain. While the consequence of some of these "hotspot" mutations (R175H, R179H, G245D, G248Q/W, Y220, R213X, R273H and R282H) has been described in solid tumors and AML, very little is known of their role in CLL. Clinically, patients with Del17p/Mutp53 have worse overall survival, increased disease progression and are more likely to relapse on the current targeted therapies such as ibrutinib. Although relapse to these treatments is largely due to acquired mutations in Bruton's Tyrosine Kinase (BTK) or its downstream target PLCg2, we hypothesize that the biology of mutant 53 bearing CLL is a key driver of resistance and progression. Specifically, we aim to determine the molecular signature and downstream effectors that allow mutant p53 to drive the adverse biology associated with this subtype. Conversely, we hypothesize that targeting the mutant p53 pathway will lead to better outcomes and overall survival for patients bearing this adverse prognosis marker. We performed high-throughput Sequencing of DNA from 270 CLL patients with high coverage in the exonic regions of TP53 prior to ibrutinib therapy as well as during progression. At baseline, 40% of patients had mutations found in the DNA binding region with the most frequent occurring in R248Q, R175H and R273H. We then characterized each p53 mutant (n=106) functionally in terms of their ability to ability to activate p21, PUMA, and Bax which serve as cell cycle checkpoint and apoptotic effectors of wild type p53 in response to DNA damage. Most mutants were incompetent in upregulating p21, PUMA or Bax at the transcript level. A few mutants upregulated p21 protein in a p53 independent fashion. We then evaluated the consequence of mutant p53 in CLL. We performed chromatin immunoprecipitation (ChIP-Seq), open chromatin signatures (ATAC-Seq) and expression analysis (RNA-Seq) on CLL samples with R248Q or R175H as well as in wild-type (WT) p53 samples. Integration of ChIP, ATAC and RNA Seq profiles indicated that mutant p53 activated a unique transcriptomic profile not shared by wt p53 bearing CLL. Several genes that facilitated survival or progression were downstream targets of mutant p53. Of these, we identified PRKCB (PKC-beta), BCL2L1 (Bcl-xL), EZH2, MLL and MALAT as a potential key downstream effectors of mutant p53. To determine whether mutations at R248Q and R175H in p53 were causal in the observed increases in PKC-beta, Bcl-xL, EZH2, MLL, and MALAT we used CRISPR/Cas9 editing to introduce mutations at R175H and R248Q in the p53 wildtype CLL cell lines HG-3 and PGA-1. These were accomplished by electroporating sgRNA-Cas9 ribonucleoprotein complexes (RNPs). Western blotting of mutants revealed an increase in the mRNA and protein expression of PKC-beta, and BCL-xL in mutant p53 compared to WT. Levels of EZH2 and MLL were not increased in these cells indicating that PKC-beta and Bcl-xL may be direct transcriptional targets upregulated by mutations at R248Q and R175H in p53. Ongoing efforts will characterize the transcriptional profile of all p53 mutants in our cohort to determine whether they all have a unifying transcriptomic profile that confers a gain of function phenotype to this subtype of CLL. Disclosures Byrd: Gilead: Other: Travel Expenses, Research Funding, Speakers Bureau; Janssen: Consultancy, Other: Travel Expenses, Research Funding, Speakers Bureau; BeiGene: Research Funding; TG Therapeutics: Other: Travel Expenses, Research Funding, Speakers Bureau; Gilead: Other: Travel Expenses, Research Funding, Speakers Bureau; Ohio State University: Patents & Royalties: OSU-2S; Novartis: Other: Travel Expenses, Speakers Bureau; Pharmacyclics LLC, an AbbVie Company: Other: Travel Expenses, Research Funding, Speakers Bureau; Genentech: Research Funding; BeiGene: Research Funding; Janssen: Consultancy, Other: Travel Expenses, Research Funding, Speakers Bureau; Ohio State University: Patents & Royalties: OSU-2S; Pharmacyclics LLC, an AbbVie Company: Other: Travel Expenses, Research Funding, Speakers Bureau; Gilead: Other: Travel Expenses, Research Funding, Speakers Bureau; TG Therapeutics: Other: Travel Expenses, Research Funding, Speakers Bureau; Janssen: Consultancy, Other: Travel Expenses, Research Funding, Speakers Bureau; Genentech: Research Funding; TG Therapeutics: Other: Travel Expenses, Research Funding, Speakers Bureau; Acerta: Research Funding; Ohio State University: Patents & Royalties: OSU-2S; Novartis: Other: Travel Expenses, Speakers Bureau; Acerta: Research Funding; BeiGene: Research Funding; Pharmacyclics LLC, an AbbVie Company: Other: Travel Expenses, Research Funding, Speakers Bureau; Acerta: Research Funding; Genentech: Research Funding; Novartis: Other: Travel Expenses, Speakers Bureau. Woyach:Janssen: Consultancy, Research Funding; Pharmacyclics LLC, an AbbVie Company: Consultancy, Research Funding; AbbVie: Research Funding; Karyopharm: Research Funding; Loxo: Research Funding; Morphosys: Research Funding; Verastem: Research Funding.

Portulaca oleracea L. extracts enhance GLUT4 translocation to the surface of muscle cells in insulin dependent and independent fashion

Portulaca oleracea L. extracts enhance GLUT4 translocation to the surface of muscle cells in insulin dependent and independent fashion

Ultrasensitive serum interferon-α quantification during SLE remission identifies patients at risk for relapse

ObjectivesMaintenance of remission has become central in the management of systemic lupus erythematosus (SLE). The importance of interferon-alpha (IFN-α) in the pathogenesis of SLE notwithstanding, its expression in remission has...

Relationship Between Level of Serum 25-Hydroxyvitamin D and Risk of Squamous Cell Carcinoma in an Iranian Population.

The relationship between vitamin D and skin squamous cell carcinoma (SCC) is not well defined. To investigate the relationship between vitamin D levels and the incidence of skin SCC for the first time in Iran. In this case-control study, 126 subjects were enrolled (63 in each group) out of referents to Razi Skin Hospital in Tehran in 2014. The risk factors for cancer gathered by self-reported questionnaires and blood samples were obtained to measure the level of 25-hydroxyvitamin D. Multivariate logistic regression was used to neutralize the effect of confounding factors. Cases of SCC were more likely to be in men, older than 49 years and working in an outdoor environment, and with longtime exposure to sunlight and a personal history of skin cancers. Family history of skin cancer and of cigarette smoking were not significantly related to SCC. In the SCC and control groups, 69.8% and 31.7%, respectively, had sufficient levels of vitamin D (P < 0.001). Mean level of 25-hydroxyvitamin D was 40.99 ng/mL in the SCC group and 26.34 ng/mL in the control group (P < 0.05). In the unadjusted model, the level of vitamin D as a continuous variable was positively related to SCC risk. In the adjusted model, vitamin D did not independently predict the likelihood of SCC. Vitamin D level and SCC risk are directly related, although not in an independent fashion. Indeed, this relation is severely confounded by exposure to sunlight, which was evidenced by an increased vitamin D level in the people working outside and the higher prevalence of SCC in the same population.

Reconciling the local and the global in the Brisbane independent fashion sector

The humid, sub-tropical city of Brisbane is Australia’s third-largest and one often regarded as culturally inferior to its southern counterparts Sydney and Melbourne. However, the city has supported a small but active independent fashion scene, and this article examines three of these businesses, exploring how they positioned themselves in relation to the global fashion industry. It contributes to literature on local fashion industries in New Zealand, Scandinavia, Canada and the United Kingdom. Challenges and opportunities presented to local fashion businesses are considered, and ways in which these have changed over time is also discussed. Case studies are drawn from a period between 1950 and 2018 and were purposively chosen so that contemporary case studies could be contextualized with historical examples. Research was conducted through archival research at the Queensland Museum, semi-structured interviews with participants and on-site observations. Findings confirm those of existing studies in the field that suggest local fashion businesses outside of large cities and dominant fashion centres may struggle to remain relevant in a fast-paced global industry, but have an opportunity to develop and foster close bonds to local cultural scenes and to contribute to place-making in the cities in which they are located.

Cutting Edge: TAK1 Safeguards Macrophages against Proinflammatory Cell Death.

TGF-β-activated kinase 1 (TAK1) is known to play vital roles for innate and adaptive immunity; however, little is known about its potential role in limiting biological responses such as inflammation. In this study, we report that macrophage TAK1 participates in negatively regulating inflammation by restraining proinflammatory cell death. Macrophages from TAK1-deficient mice underwent cell death in response to LPS and poly(I:C), which took place in a manner dependent on TLR/TRIF-induced active Caspase8-mediated cleavage of gasdermin D, known as an executioner of pyroptosis. Likewise, TNF-α induced Caspase8-dependent gasdermin D processing following cell death in TAK1-deficient macrophages. Importantly, we demonstrated that this type of proinflammatory macrophage death is linked to susceptibility to septic shock in mice lacking TAK1 in macrophages in a TNF-α-independent fashion. Taken together, our data revealed that TAK1 acts as a signaling checkpoint to protect macrophages from unique proinflammatory cell death, ensuring the maintenance of innate immune homeostasis.

Von Expeditionsberichten zum Führer — die Anfänge der Ratgeber für Riesengebirgereisende an der Jahrhundertwende des 18. zum 19. Jahrhundert

From travel accounts to guidebooks: The beginnings of guidebooks to the Giant Mountains Karkonosze for travellers in the late 18th and early 19th centuryIn the history of European tourism the Giant Mountains Karkonosze occupy a unique place thanks to the Chapel of St. Lawrence, funded by Count Christoph Leopold Schaffgotsch and located on the summit of Śnieżka. Its construction in the Habsburg dominions in the turbulent period of the Counter-Reformation was meant to finally put an end to the Silesian-Bohemian border dispute and become a visible sign of Catholic rule over the highest mountain range of the two neighbouring countries. The construction of the chapel also marked the beginning of tourism in the highest range of the Sudetes; initially, its nature was religious and focused on pilgrimages to the summit of Śnieżka, featuring, in addition to local inhabitants, also sanatorium visitors to Cieplice Warmbrunn, which was owned by the Schaffgotschs.After the three Silesian Wars, as a result of which the lands to the north of the mountains were separated from the Habsburgs’ Kingdom of Bohemia, the situation in the region changed radically. The Counter-Reformation pressure ceased and the Lutherans began to grow in importance, supported as they were by the decidedly pro-Protestant Prussian state, governed by its tolerant monarch.The period was also marked by an unprecedented growth in the literature on the Giant Mountains — there were poems Tralles, nature studies Volkmar and travel accounts GutsMuths, Troschel and others written about the highest range of the Sudetes. A special role among these writings was played by works aimed at introducing the public from the capital Berlin to the new province of the Kingdom of Prussia, especially to the mountains, so exotic from the point of view of the “groves and sands” of Brandenburg. These publications were written primarily by Lutheran clergymen, which was not without significance to the nature of the works. This was also a time when the first guidebooks to the Giant Mountains were written, with many of their authors also coming from the same milieu.What emerges from this image is a kind of confessionalisation of tourism in the highest mountains of Silesia and Bohemia: on the one hand there are mass Catholic pilgrimages and on the other — a new type of individual tourists who, with a book in hand, traverse mountain paths in a decidedly more independent fashion.

Od relacji z wypraw do przewodnika — początki karkonoskich poradników dla podróżnych na przełomie XVIII i XIX wieku

From travel accounts to guidebooks: The beginnings of guidebooks to the Giant Mountains Karkonosze for travellers in the late 18th and early 19th centuryIn the history of European tourism the Giant Mountains Karkonosze occupy a unique place thanks to the Chapel of St. Lawrence, funded by Count Christoph Leopold Schaffgotsch and located on the summit of Śnieżka. Its construction in the Habsburg dominions in the turbulent period of the Counter-Reformation was meant to finally put an end to the Silesian-Bohemian border dispute and become a visible sign of Catholic rule over the highest mountain range of the two neighbouring countries. The construction of the chapel also marked the beginning of tourism in the highest range of the Sudetes; initially, its nature was religious and focused on pilgrimages to the summit of Śnieżka, featuring, in addition to local inhabitants, also sanatorium visitors to Cieplice Warmbrunn, which was owned by the Schaffgotschs.After the three Silesian Wars, as a result of which the lands to the north of the mountains were separated from the Habsburgs’ Kingdom of Bohemia, the situation in the region changed radically. The Counter-Reformation pressure ceased and the Lutherans began to grow in importance, supported as they were by the decidedly pro-Protestant Prussian state, governed by its tolerant monarch.The period was also marked by an unprecedented growth in the literature on the Giant Mountains — there were poems Tralles, nature studies Volkmar and travel accounts GutsMuths, Troschel and others written about the highest range of the Sudetes. A special role among these writings was played by works aimed at introducing the public from the capital Berlin to the new province of the Kingdom of Prussia, especially to the mountains, so exotic from the point of view of the “groves and sands” of Brandenburg. These publications were written primarily by Lutheran clergymen, which was not without significance to the nature of the works. This was also a time when the first guidebooks to the Giant Mountains were written, with many of their authors also coming from the same milieu.What emerges from this image is a kind of confessionalisation of tourism in the highest mountains of Silesia and Bohemia: on the one hand there are mass Catholic pilgrimages and on the other — a new type of individual tourists who, with a book in hand, traverse mountain paths in a decidedly more independent fashion.

A stochastic model of ion channel cluster formation in the plasma membrane.

Ion channels are often found arranged into dense clusters in the plasma membranes of excitable cells, but the mechanisms underlying the formation and maintenance of these functional aggregates are unknown. Here, we tested the hypothesis that channel clustering is the consequence of a stochastic self-assembly process and propose a model by which channel clusters are formed and regulated in size. Our hypothesis is based on statistical analyses of the size distributions of the channel clusters we measured in neurons, ventricular myocytes, arterial smooth muscle, and heterologous cells, which in all cases were described by exponential functions, indicative of a Poisson process (i.e., clusters form in a continuous, independent, and memory-less fashion). We were able to reproduce the observed cluster distributions of five different types of channels in the membrane of excitable and tsA-201 cells in simulations using a computer model in which channels are "delivered" to the membrane at randomly assigned locations. The model's three parameters represent channel cluster nucleation, growth, and removal probabilities, the values of which were estimated based on our experimental measurements. We also determined the time course of cluster formation and membrane dwell time for CaV1.2 and TRPV4 channels expressed in tsA-201 cells to constrain our model. In addition, we elaborated a more complex version of our model that incorporated a self-regulating feedback mechanism to shape channel cluster formation. The strong inference we make from our results is that CaV1.2, CaV1.3, BK, and TRPV4 proteins are all randomly inserted into the plasma membranes of excitable cells and that they form homogeneous clusters that increase in size until they reach a steady state. Further, it appears likely that cluster size for a diverse set of membrane-bound proteins and a wide range of cell types is regulated by a common feedback mechanism.

P120 inhibits LPS/TNFα-induced endothelial Ang2 synthesis and release in an NF-κB independent fashion

Adherens junction protein p120 is thought to be crucial for maintaining vascular integrity, which is important in many pathologies and diseases including atherosclerosis, vascular malformations, hemorrhagic stroke, sepsis and others. However, the mechanisms responsible for this is not completely understood. In this study, using an unbiased proteomics approach, followed by other experimental techniques, we identified that in HUVECs p120 overexpression inhibits LPS/TNFα-induced angiopoietin-2 (Ang2) expression, a key switch of endothelial destabilization. Interestingly, p120 overexpression did not inhibit LPS/TNFα-induced expression of adhesion molecules/cytokines including VCAM-1, ICAM-1, E-selectin, MCP-1, IL-8 and IL-6 in our experimental system. Furthermore, this p120-mediated repression of Ang2 is in an NF-κB independent manner, possibly via transcription factor Ets1. Our results demonstrate that p120 influences vascular integrity by secreted signals, providing new insights into the mechanisms of p120-mediated vascular stability.

Gendered Entrepreneurialism and the Labour of Online Consumption in the Independent Fashion Sector

In the intersecting space between contemporary economic models, social media, and fashion and the body, it has been suggested that women are performing emotional and esthetic labor with increasing scrutiny, intensity and impact. This article considers interactions between and within two Instagram accounts from the perspective of these intersecting issues, using the labor they perform within the context of online consumption as a case study. The purpose is first to understand how these interactions occur in online contexts; and secondly, to examine what insight these interactions can offer into gendered labor and entrepreneurialism in fashion. Data was collected over a 12-month period in which the Instagram accounts of the fashion label Elizabeth Suzann and an Instagram community dedicated to her clothing were observed. Findings highlight the centrality of the body in their interactions and it is proposed that the accounts studied reflect a type of gendered entrepreneurialism in which esthetic and emotional labor is performed in order to create a welcoming, safe and useful space - especially for women whose bodies do not conform to social and industry norms.

Regulation of cardiac fibroblast-mediated maladaptive ventricular remodeling by β-arrestins.

Cardiac fibroblasts (CF) play a critical role in post-infarction remodeling which can ultimately lead to pathological fibrosis and heart failure. Recent evidence demonstrates that remote (non-infarct) territory fibrosis is a major mechanism for ventricular dysfunction and arrhythmogenesis. β-arrestins are important signaling molecules involved in β-adrenergic receptor (β-AR) desensitization and can also mediate signaling in a G protein independent fashion. Recent work has provided evidence that β-arrestin signaling in the heart may be beneficial, however, these studies have primarily focused on cardiac myocytes and their role in adult CF biology has not been well studied. In this study, we show that β-arrestins can regulate CF biology and contribute to pathological fibrosis. Adult male rats underwent LAD ligation to induce infarction and were studied by echocardiography. There was a significant decline in LV function at 2–12 weeks post-MI with increased infarct and remote territory fibrosis by histology consistent with maladaptive remodeling. Collagen synthesis was upregulated 2.9-fold in CF isolated at 8 and 12 weeks post-MI and β-arrestin expression was significantly increased. β-adrenergic signaling was uncoupled in the post-MI CF and β-agonist-mediated inhibition of collagen synthesis was lost. Knockdown of β-arrestin1 or 2 in the post-MI CF inhibited transformation to myofibroblasts as well as basal and TGF-β-stimulated collagen synthesis. These data suggest that β-arrestins can regulate CF biology and that targeted inhibition of these signaling molecules may represent a novel approach to prevent post-infarction pathological fibrosis and the transition to HF.

Media Accountability: Critical Analysis Of Citizen Journalism

The media is considered to be the fourth pillar in a democratic country. It acts as an effective control mechanism to check the other branches of the government. But this is only consequential when the media functions in an independent and transparent fashion with trained and neutral professionals who are aware of the accountability and consequences of their work. All these factors together would further the country as a democratic institution. Traditionally, it was legacy media responsible for a one-to-many communication process. Their goal was to provide information to the citizens. But this changed with development in technology and the use of social media in daily life. The internet brought with it new media formats which are easily accessible but also unstructured. These lowered barriers of entry in the media enabled citizens to become active participants in the communication process. As a result, these citizens developed a different relationship with the already existing media wherein they were not only the receivers to information but also co-producers. Real-time information allows users to communicate with each other and in turn widely generate public opinion on internet platforms. A many-to-many communication style emerged. While on the one hand, this type of discourse could be an opportunity for citizens to exercise their fundamental freedom of speech and expression, it is on the other hand, proving to have a detrimental effect in two parts: Lack of neutrality, polarized views and pre-existing misconceptions on the part of citizens as well as algorithms and formation of echo-chambers on the part of technology. Some questions arise in this scenario about the capability of citizen journalists, the duties they should adhere to along with the enjoyment of their rights and freedoms, the risks involved in an unchecked method of communication and the effect of citizen journalism in the democratic process.

Crafting symbolic value: art, craft and independent fashion

It has been suggested that independent fashion exists at the intersection of ‘art, craft and fashion’ implying that both its identity and its value emerge directly from its connection with these adjacent cultural fields. But what are the precise circumstances under which independent fashion ‘intersects’ with the art and craft sectors and how exactly is symbolic value negotiated along cultural value chains? This article uses a case study approach to examine interactions between the independent fashion sector and arts institutions. Three exhibitions are provided as case studies, and were selected for analysis on the basis of their shared links between art, craft, and fashion design as forms of visual culture and personal expression. Findings suggest that arts institutions and curators play an essential role in validating the cultural value of independent fashion in ways that help distinguish independent fashion from the anonymity of industrially produced garments and establish the independent fashion sector as a vital player in the creative industries. However, as the paper also shows, the mechanisms through which fashion comes to be exhibited in galleries relies on complex set of social and professional interactions.

CD45Rb-low effector T cells require IL-4 to induce IL-10 in FoxP3 Tregs and to protect mice from inflammation.

CD4+ effector/memory T cells (Tem) represent a leading edge of the adaptive immune system responsible for protecting the body from infection, cancer, and other damaging processes. However, a subset of Tem cells with low expression of CD45Rb (RbLoTem) has been shown to suppress inflammation despite their effector surface phenotype and the lack of FoxP3 expression, the canonical transcription factor found in most regulatory T cells. In this report, we show that RbLoTem cells can suppress inflammation by influencing Treg behavior. Co-culturing activated RbLoTem and Tregs induced high expression of IL-10 in vitro, and conditioned media from RbLoTem cells induced IL-10 expression in FoxP3+ Tregs in vitro and in vivo, indicating that RbLoTem cells communicate with Tregs in a cell-contact independent fashion. Transcriptomic and multi-analyte Luminex data identified both IL-2 and IL-4 as potential mediators of RbLoTem-Treg communication, and antibody-mediated neutralization of either IL-4 or CD124 (IL-4Rα) prevented IL-10 induction in Tregs. Moreover, isolated Tregs cultured with recombinant IL-2 and IL-4 strongly induced IL-10 production. Using house dust mite (HDM)-induced airway inflammation as a model, we confirmed that the in vivo suppressive activity of RbLoTem cells was lost in IL-4-ablated RbLoTem cells. These data support a model in which RbLoTem cells communicate with Tregs using a combination of IL-2 and IL-4 to induce robust expression of IL-10 and suppression of inflammation.

Communicating material characteristics in a digital age: Three case studies in independent fashion

This article focuses on the intersection of the material and the digital in the independent fashion sector, with a focus on ways in which digital media and contemporary communication tools are being used to unite them. It explores the tension between the opportunities provided to small business in the contemporary media landscape and its use in a sector closely associated with a nostalgic valorisation of material qualities related to nature, artisanship and luxury. It further examines ways in which the communication of material characteristics through technological platforms contributes symbolic value to independent fashion as a cultural product. Thematic analysis was conducted of online texts and images related to three contemporary American independent labels in a three-year period (2014 –2017). The study finds that instead of being viewed as a threat to the independent fashion sector’s driving ethos, technology has been embraced as a tool allowing independent fashion producers to amplify their voices as they challenge existing fashion paradigms.

Shifts of Spatial Attention in Visual and Tactile Working Memory are Controlled by Independent Modality-Specific Mechanisms.

The question whether the attentional control of working memory (WM) is shared across sensory modalities remains controversial. Here, we investigated whether attention shifts in visual and tactile WM are regulated independently. Participants memorized visual and tactile targets in a first memory sample set (S1) before encoding targets in a second sample set (S2). Importantly, visual or tactile S2 targets could appear on the same side as the corresponding S1 targets, or on opposite sides, thus, requiring shifts of spatial attention in visual or tactile WM. The activation of WM representations in modality-specific visual and somatosensory areas was tracked by recording visual and tactile contralateral delay activity (CDA/tCDA). CDA/tCDA components emerged contralateral to the side of visual or tactile S1 targets, and reversed polarity when S2 targets in the same modality appeared on the opposite side. Critically, the visual CDA was unaffected by the presence versus absence of concurrent attention shifts in tactile WM, and the tactile CDA remained insensitive to visual attention shifts. Visual and tactile WM performance was also not modulated by attention shifts in the other modality. These results show that the dynamic control of visual and tactile WM activation processes operates in an independent modality-specific fashion.