Increasing Storage Duration Research Articles

Platelet Storage Lesions in Second‐Generation Containers: Correlation with in vivo Behavior with Storage up to 14 Days

The relationship between in vivo behavior and in vitro characteristics of 59 platelet concentrates (PC) stored for up to 14 days in a synthetic medium or in CPDA-1 plasma was systematically investigated. 25 paired studies (1 study was incomplete) were performed comparing platelets suspended either in the synthetic medium or CPDA-1 plasma with 5 days (n = 5); 7 days (n = 10); 10 days (n = 5); and 14 days (n = 5) of storage. In addition, 10 control studies were performed with freshly prepared PC (6-24 h) in CPDA-1 plasma. Both percent recovery and survival estimations showed decreases with increasing storage duration, irrespective of storage medium used. In both media, with prolonged storage, the platelet survival curves not only became shorter, but also increasingly exponential, suggesting that in vitro storage caused progressive damage to the platelets present in circulation. Survival curves of platelets suspended in synthetic medium remained more linear, indicative of less random damage during storage. Mean population lifespan (MPL) of the stored PC was determined by the area below the survival curve divided by the mean percent recovery for the fresh PC, which was 55%. MPL decreased from 4.5 days (fresh PC) to 0.4 days after 14 days of storage in plasma, with a 50% reduction (t1/2) estimated at 7.2 days of storage. MPL t1/2 for PC stored in the synthetic medium was estimated to be 8.8 days.(ABSTRACT TRUNCATED AT 250 WORDS)

Platelet Storage Lesions in Second-Generation Containers: Correlation with in vivo Behavior with Storage up to 14 Days

The relationship between in vivo behavior and in vitro characteristics of 59 platelet concentrates (PC) stored for up to 14 days in a synthetic medium or in CPDA-1 plasma was systematically investigated. 25 paired studies (1 study was incomplete) were performed comparing platelets suspended either in the synthetic medium or CPDA-1 plasma with 5 days (n = 5); 7 days (n = 10); 10 days (n = 5); and 14 days (n = 5) of storage. In addition, 10 control studies were performed with freshly prepared PC (6-24 h) in CPDA-1 plasma. Both percent recovery and survival estimations showed decreases with increasing storage duration, irrespective of storage medium used. In both media, with prolonged storage, the platelet survival curves not only became shorter, but also increasingly exponential, suggesting that in vitro storage caused progressive damage to the platelets present in circulation. Survival curves of platelets suspended in synthetic medium remained more linear, indicative of less random damage during storage. Mean population lifespan (MPL) of the stored PC was determined by the area below the survival curve divided by the mean percent recovery for the fresh PC, which was 55%. MPL decreased from 4.5 days (fresh PC) to 0.4 days after 14 days of storage in plasma, with a 50% reduction (t y) estimated at 7.2 days of storage. MPL ty for PC stored in the synthetic medium was estimated to be 8.8 days. The decrease in in vivo viability with prolonged storage was paralleled with loss of energy-dependent in vitro parameters such as hypotonic shock response, shape change with ADP, and ATP levels, and with increased lactate levels. Although the length of the storage period was shown to be the major factor responsible for the variability in MPL observed in this study, multiple linear-regression analysis showed also that platelet discoid shape, as measured by the shape change, and lactate production were independent in vitro parameters with significant predictability of the in vivo viability. Combined with days of storage in the regression equation, these parameters were found to ‘explain’ 78% of the variability of the MPL found in this study.

Physiological Changes in Yellow Summer Squash at Chilling and Nonchilling Temperatures

Abstract Summer squash (Cucurbita pepo L.) fruit were harvested 8 ± 1 day after anthesis and held at 2 or 15C. Samples were transferred from 2 to 15C after 3, 6, 9, or 12 days of storage. Respiration and ethylene production were measured 24 and 48 hr after fruit had been transferred to 15C. Electrolyte leakage from pericarp disks was determined 48 hr after transfer to 15C. Weight loss during storage was greater at 15 than at 2C; however, chilled fruit had a greater rate of weight loss after transfer to 15C than did nonchilled fruit. Surface pitting became obvious after 6 days at 2C and was progressively more severe with increased duration of chilling. Respiration rates of nonchilled fruit decreased with increased storage duration, whereas respiration rates of chilled fruit increased with increased storage duration. Ethylene production was <0.1 nl g-1hr-1 in nonchilled fruit. Ethylene production was enhanced in chilled fruit and increased with increased duration of chilling to a peak of 1.5 nl-g-1-hr-1 after 9 days at 2C, then decreased. Electrolyte leakage was not influenced by chilling.

Progressive loss of fibronectin-mediated opsonic activity in plasma cryoprecipitate with storage. Role of fibronectin fragmentation.

Septic injured patients often manifest a deficiency of plasma fibronectin. Several studies have shown improvements in organ function in such patients following infusion of fibronectin-rich plasma cryoprecipitate, while other studies found no improvement. One explanation for these differences may be the use of plasma cryoprecipitate which has been stored for various time intervals prior to its use as a source of fibronectin. This investigation tested the hypothesis that the opsonic activity of fibronectin in cryoprecipitate may decline with increased storage duration. Using a bioassay of opsonic activity, we evaluated human plasma cryoprecipitate that was stored at either -20 or -80 degrees C for various intervals (2 weeks to 12 months) after its preparation from fresh donor plasma. Our findings demonstrated that the opsonic activity of fibronectin in cryoprecipitate declined with increasing time of storage. Significant loss (p less than 0.05) of opsonic activity was first evident after 2 months of storage. Storage at -80 degrees C did not prevent this decline in opsonic activity as compared to storage at -20 degrees C. Immunoblot analysis revealed extensive fragmentation of the dimeric fibronectin (440 kdaltons) and the presence of lower molecular weight fragments in 4- to 12-month-old plasma cryoprecipitate. Therefore, plasma cryoprecipitate of varying ages (storage time) when used as a source of fibronectin for replacement therapy to support phagocytic function in septic injured patients may result in different fibronectin-mediated responses. The decline in activity may be due, in part, to fragmentation of the fibronectin molecule.

SIZE- AND SEX-RELATED EMERGENCE, AND SURVIVAL IN COLD STORAGE, OF MOUNTAIN PINE BEETLE ADULTS

AbstractLarvae of the mountain pine beetle, Dendroctonus ponderosae Hopk., were reared to adults in naturally infested bolts of lodgepole pine, Pinus conforta Dougl. var. latifolia Engelm., at 21 ± 3 °C. The mean pronotal width of the emerging beetles of both sexes decreased, and the male:female ratio increased, during the emergence period. On the average, larger individuals of both sexes survived longer when adult beetles were stored at 1 ± 2 °C. Also, the male:female ratio of the surviving beetles decreased with increased storage duration. These and related results in the literature suggest that in natural populations of the mountain pine beetle average adult size increases, and the male:female ratio decreases, following stress-induced mortality in the larval and(or) adult stages.