This article presents the results of laboratory examinations concerning the effects of soil bulk density on the growth of sessile oak Quercus petraea Liebl. seedlings. The soil for the study was collected from a nursery plot and then compacted into PCV pots in eight different bulk density levels ranging from 0.81 to 1.32 g cm−3. Oak seedlings were cultivated in controlled conditions for 76 days after sowing. The growth and mass parameters of particular seedlings together with the parameters of the assimilation apparatus and roots were measured, taking into account the share of various diameter roots in the root system. For the purpose of the experiment, 120 acorns were sowed, of which 73 seedlings were cultured. An analysis of the growth of the sessile oak seedlings revealed that a change in soil density significantly affected root system development, total height of seedlings and dry mass. It was observed that the dry mass of the root system as well as the area and length of the roots decreased with an increase in soil density. Root system reduction was noted, the size of which determines the proper development of the tree and ensures its stability. Besides a decrease in the root system, an increase in soil compaction also resulted in a reduction in the number of roots of a specified diameter. The first to be affected were the smallest roots, i.e., those with diameters up to 0.2 mm, which are responsible for the uptake of mineral components. It was confirmed that even a slight increase in soil compaction might negatively result in the growth of young seedlings, impeding root system development.