Pineapple Liquid Waste (PLW) is waste that has acidity characteristics and contains organic material which can be utilized as a substrate for bacterial growth. Researchers are trying to provide the latest breakthroughs to utilize Pineapple Waste (PLW) as organic fertilizer to reduce waste increases and increase agricultural yields. The purpose of this research is to find out the effect on the weight of pineapple and find out whether it will increase vitamin C levels in pineapple. This study used an Experimental Method, the design of the design used was a Complete Random Design Variety and analyzed using a multivariate test. the conclusion of this study is the variation of PLW has a significant effect on fruit weight p> 0.05 and on vitamin C levels p < 0.05, so that no significant effect on fruit weight and significant effect on increasing levels of vitamin C, so this research can potentially design learning resources for biology.