Background/aims Core training has been recommended in shoulder rehabilitation programs. However, the evidence on this topic is still scarce. The aim of this study was to investigate the effect of conscious abdominal contraction combined with unstable surfaces on electromyographic activity of periscapular muscles during the plus phase of a push-up. Methods A total of 20 male participants (22.8 ± 2.5 years) were evaluated. Electromyographic signals were collected from the upper, middle and lower trapezius, serratus anterior, and external and internal oblique muscles during push-up exercises on a stable and unstable surface with and without the conscious abdominal contraction. Each participant performed four variations of the plus phase of a push-up. Results The results demonstrated that the conscious abdominal contraction caused an increase in the electromyographic activity of external oblique, internal oblique, serratus anterior and lower trapezius muscles (P≤0.008). The unstable surface caused only a relevant increase in electromyographic activity of the abdominal muscles (P≤0.025). Combining the two strategies did not increase the electromyographic activity of any muscles (P≥0.238). Conclusions The conscious abdominal contraction seems to be a viable strategy to increase the electromyographic activity of the periscapular muscles.