Increasing Vapor Pressure Deficit Research Articles

Constant hydraulic supply and ABA dynamics facilitate the trade-offs in water and carbon.

Carbon-water trade-offs in plants are adjusted through stomatal regulation. Stomatal opening enables carbon uptake and plant growth, whereas plants circumvent drought by closing stomata. The specific effects of leaf position and age on stomatal behavior remain largely unknown, especially under edaphic and atmospheric drought. Here, we compared stomatal conductance (gs ) across the canopy of tomato during soil drying. We measured gas exchange, foliage ABA level and soil-plant hydraulics under increasing vapor pressure deficit (VPD). Our results indicate a strong effect of canopy position on stomatal behavior, especially under hydrated soil conditions and relatively low VPD. In wet soil (soil water potential > -50 kPa), upper canopy leaves had the highest gs (0.727 ± 0.154 mol m-2 s-1) and assimilation rate (A; 23.4 ± 3.9 µmol m-2 s-1) compared to the leaves at a medium height of the canopy (gs : 0.159 ± 0.060 mol m2 s-1; A: 15.9 ± 3.8 µmol m-2 s-1). Under increasing VPD (from 1.8 to 2.6 kPa), gs , A and transpiration were initially impacted by leaf position rather than leaf age. However, under high VPD (2.6 kPa), age effect outweighed position effect. The soil-leaf hydraulic conductance was similar in all leaves. Foliage ABA levels increased with rising VPD in mature leaves at medium height (217.56 ± 85 ng g-1 FW) compared to upper canopy leaves (85.36 ± 34 ng g-1 FW). Under soil drought (< -50 kPa), stomata closed in all leaves resulting in no differences in gs across the canopy. We conclude that constant hydraulic supply and ABA dynamics facilitate preferential stomatal behavior and carbon-water trade-offs across the canopy. These findings are fundamental in understanding variations within the canopy, which helps in engineering future crops, especially in the face of climate change.

Vegetation growth due to CO2 fertilization is threatened by increasing vapor pressure deficit

Numerous studies found that the CO2 fertilization effect can enhance vegetation growth, however, some recent studies showed that the increase of vapor pressure deficit (VPD) could reduce vegetation growth due to an increase in surface resistance. It remains unclear to what extent VPD increases can offset the CO2 fertilization effect. Here, we examined the long-term trends of terrestrial gross primary productivity (GPP) at the global scale using six products derived from satellite observations, machine learning algorithms, and dynamic vegetation model simulations. While we found significant increases (p less than 0.05) in GPP in most of the world, we also found significant decreases in GPP over the Amazon basin, western North America, eastern Europe and central Asia. Our attribution analysis showed that although the elevated CO2 concentration dominated the long-term trends of GPP, VPD also played an important role. The increasing VPD could explain the decreasing GPP over the arid and tropical regions. The negative contribution of VPD to GPP trends appeared to become amplified with time, leading to suppressed global vegetation growth in the last two decades. The amplified contribution of VPD to GPP trends was directly related to the decrease in soil moisture, indicating the soil moisture-induced land–atmosphere coupling (LAC) and the vegetation growth stagnation since the year 2000. Our results provide insight into the negative contribution of VPD to long-term GPP trends, which can partly offset 68.21 % of the CO2 fertilization effect and even stagnate the vegetation growth with time. The possible mechanisms behind the effect of soil moisture-VPD coupling on the vegetation dynamics at the global scale needs further investigation.

Near constant groundwater recharge efficiency under global change in a central European catchment

AbstractThe fraction of precipitation that infiltrates soils and subsequently becomes recharge is one of the principle components of an unconfined aquifer's water budget (this fraction is here termed recharge efficiency). Here we tested how recharge efficiency will respond to climate change including a possible plant physiological response to climate change (e.g., stomatal closure; increasing leaf area) in a catchment used for drinking water production in western Germany. To this end we used a soil water model (HYDRUS‐1D) forced with climate data spanning the time period from 1971 to 2099. Three different vegetation types were considered: turf grass representing the primary infiltration sites within residential areas; maize representing the main crop on agriculturally used land; and beech representing the forested parts of the catchment. We found that, the positive effects of climate change on recharge efficiency (more rain during the main recharge season in winter, less crop water demand due to faster plant ripening in spring and summer, increased plant water use efficiency, reduced global radiation as cloud density increases) were not completely compensated by the negative effects (less precipitation, higher leaf area index and increasing vapour pressure deficit in summer season) at our study site. Because total annual precipitation increased slightly until the end of the 21th century, changes in the amount of total annual recharge were also positive, though moderate (up to +20% change in the period 2071–2099 as compared to 1970–2000). The results of this study will be helpful for water authorities managing water rights under the perspective of a changing climate. In the future, our study site is expected to receive sufficient recharge from precipitation to maintain current rates of groundwater withdrawal for public water supply and irrigation. Thus, the region's agriculture sector may become a ‘global warming winner,’ when cropping in other regions in Europe may increasingly suffer from drying conditions during the growing season.

Carbon uptake by Douglas-fir is more sensitive to increased temperature and vapor pressure deficit than reduced rainfall in the western Cascade Mountains, Oregon, USA

Understanding how trees respond to drought is critical to understanding forest sensitivity to global climate change, which can help inform forest policy and management decisions. However, mechanisms governing carbon fixation and water fluxes in response to increased temperatures and water limitation in regions with Mediterranean climates, with wet winters and dry summers, remain only partially understood. We tested the effect of increased vapor pressure deficit (VPD) and decreased rainfall on water and carbon fluxes of Douglas-fir (Pseudotsuga menziesii) trees using the Soil-Plant-Atmosphere model (SPA). We simulated a 50-year-old Douglas-fir stand on the western slopes of the Cascade Mountains in Oregon, USA. Simulation results showed that increasing the daily maximum VPD by 0.25–2.5 kPa during the summer increased cumulative transpiration by 1–3% and decreased cumulative gross primary production by 3–25%. In contrast, decreasing rainfall by 10–100% during the spring and summer decreased cumulative transpiration by 2–16% and decreased cumulative gross primary production by 0.5–4%. Transpiration was highly sensitive to decreases in rainfall, especially in late spring and early summer but much less sensitive to increases in maximum daily VPD. In contrast, gross primary productivity was much more sensitive to VPD, with summertime increases in VPD having a 5- to 6-fold greater effect on gross primary productivity than did decreasing the rainfall. These results suggested that temperature increases expected from climate change in combination with increases in VPD are likely to reduce forest productivity regardless of soil moisture availability.

Climate Controls on the Spatial Variability of Vegetation Greenup Rate across Ecosystems in Northern Hemisphere.

Variations in individual phenological events in response to global change have received considerable attentions. However, the development of phenological stages is relatively neglected, especially based on in situ observation data. In this study, the rate of vegetation greenup (Vgreenup) across the Northern Hemisphere was examined for different plant functional types (PFTs) by using eddy covariance flux data from 40 sites (417 site-years). Then, the controls of climatic variables on the spatial distribution of Vgreenup across PFTs were further investigated. The mean Vgreenup was 0.22 ± 0.11 g C m-2 day-2 across all sites, with the largest and lowest values observed in cropland and evergreen needle-leaf forest, respectively. A strong latitude dependence by Vgreenup was observed in both Europe and North America. The spatial variations of Vgreenup were jointly regulated by the duration of greenup (Dgreenup) and the amplitude of greenup (Agreenup). However, the predominant factor was Dgreenup in Europe, which changed to Agreenup in North America. Spring climatic factors exerted significant influences on the spatial distribution of Vgreenup across PFTs. Specifically, increasing temperature tended to shorten Dgreenup and promote Agreenup simultaneously, resulting in an acceleration of Vgreenup. Dryness had a depression effect on Vgreenup for the whole study area, as exhibited by a lower Vgreenup with increasing vapor pressure deficit or decreasing soil moisture. However, Vgreenup in North America was only significantly and positively correlated with temperature. Without the limitation of other climatic factors, the temperature sensitivity of Vgreenup was higher in North America (0.021 g C m-2 day-2 °C-1) than in Europe (0.015 g C m-2 day-2 °C-1). This study provides new cognitions for Vgreenup dynamics from in situ observations in complement to satellite observations, which can improve our understanding of terrestrial carbon cycles.

Disintegrating the impact of climate change on maize yield from human management practices in China

Climate warming has posed one of the greatest threats to global food security during the 21st century. Evidence suggests that such a threat could be partly offset by promoting food production through human management. Most studies have focused on quantifying the impact of climatic factors on food quantity. However, the contributions of human management practices, including fertilization and irrigation, to food production are yet to be comprehensively understood. Using integrated information on climate, fertilizer use, and irrigation water allocation across China's 208 prefecture-level administrative divisions, as well as four spatial panel models, we have successfully disintegrated the relative effects of climate change and human management practices on China's maize yield. From 1982 to 2013, the average maize yield in maize planting regions in China increased by 1.7 times. The overall impact of human management (fertilizer use and irrigation) on maize yield was higher than that of climate change (based on the optimal spatial Durbin model where the coefficients of most factors were significant at p < 0.01; R2 = 0.65). The vapor pressure deficit (VPD) was the main influencing factor on maize yield followed by nitrogen and phosphorus (N&P) fertilizer use, precipitation, and diurnal temperature range (DTR) respectively. For example, maize yield would decrease by 15.0% per 0.1 kPa increase in VPD and increase by 29% per 10 g added in N&P fertilizer per square meter. Specifically, the VPD negatively influenced the maize yield in the Northern spring and the Southwest maize region, while the increased temperature negatively influenced the maize yield in the Huang-Huai spring-summer maize region. The N&P fertilizer use, precipitation, and diurnal temperature range all positively influenced the maize yield. N&P fertilizer use in the South maize region had a greater effect on improving maize yield than the other regions. The yield in the South maize region benefited more from human activities. This research could provide a timely reference for improving food production in China's maize planting regions and maintaining food security.

Water use dynamics of trees in a Pinus tabuliformis plantation in semiarid sandy regions, Northeast China

Quantifying tree water use and its environmental controlling mechanisms are significantly important for understanding of adaptation strategy of trees and plantation management in semiarid and arid regions. Here, tree water consumption and tree conductance of Chinese pine (Pinus tabuliformis) plantation were quantified using sap flow measurements. Environmental variables were measured concurrently during the growing seasons of 2019 and 2020. Results showed that daily, daytime and nighttime tree water consumption averaged 27.4, 24.7, and 2.7 kg d-1 across two years, respectively. Nighttime tree water consumption accounted for 10% of the daily’s, which was mainly used for recharging stem water storage deficit caused by daytime transpiration to avoid hydraulic failure. Daytime tree water consumption was more driven by solar radiation than by vapor pressure deficit. Moreover, daytime tree conductance was 0.9 mm s-1, and significantly decreased with increasing vapor pressure deficit and decreasing soil moisture, indicating that trees had strict stomatal regulation on tree transpiration. Scaling-up from tree to stand transpiration, daytime stand transpiration averaged 1.1 mm d-1 across two years, while accumulated stand transpiration was 112.8 mm and 126.8 mm in 2019 and 2020, respectively, accounting for 33% and 37% of precipitation. However, soil evaporation occupied 35% and 51% of precipitation in 2019 and 2020, respectively. This indicated that most of water in the plantation was consumed by soil evaporation. Difference between precipitation and sum of daytime stand transpiration, canopy interception, soil evaporation, and change in soil water storage was positive in two years, indicating that water budget was balanced. These findings indicated that Chinese pine trees had strict stomatal regulation on tree transpiration, and could recharge stem water storage deficit by nighttime sap flow. Moreover, stand transpiration occupied a small part of the water balance, and thus water supply was surplus, resulting in maintaining the stability of Chinese pine plantation.

Vapor pressure deficit constrains transpiration and photosynthesis in holm oak: A comparison of three methods during summer drought.

High rates of vapor pressure deficit (VPD) can severely decrease plant productivity by reducing stomatal conductance, which might be exacerbated during Mediterranean summers due to soil water deficit. In this study, we monitored the response of holm oak, the archetype of Mediterranean trees, to changes in VPD during a summer drought period to evaluate the effects and consequences on gas exchange of the two water stresses (atmospheric and soil). Measurements were performed on trees growing in an experimental plantation over two summers with moderate drought stress by using three different methods: at the leaf level with an infrared gas analyzer, using a whole-plant chamber for short-term monitoring at the tree level, and measuring the canopy temperature for long-term monitoring. The three methods provided negative relationships between leaf conductance and VPD but with discrepancies probably associated with the measurement scale. Overall, the results showed that atmospheric and soil water stress had an additive effect. Under well-watered conditions, an increase in VPD was partially compensated by a reduction in stomatal conductance, resulting in a slight increase in the transpiration rates. With soil water deficit, the response to VPD resulted in a further decrease in stomatal conductance, reducing transpiration as a water saving strategy. The decrease in conductance in response to VPD was transitory, recovering to initial values as soon as the VPD decreased, both under well-watered and drought conditions. Due to this high sensitivity to atmospheric drought, the maximum carbon gain rates of holm oak were restricted to a short environmental window, which might modulate its physiological performance and natural distribution.

The divergence of micrometeorology sensitivity leads to changes in GPP/SIF between cork oak and poplar

The observation of solar-induced chlorophyll fluorescence (SIF) signals on a spaceborne platform provides a new idea for estimating and monitoring the gross primary production (GPP) of terrestrial ecosystems at the regional scale. To improve the accuracy of remote sensing SIF signals to estimate GPP, the combination of ground tower-based continuous SIF signals with GPP to explore the mechanical connection in different ecosystems is needed. Based on continuous ground observations, the discrepancy of the links between SIF and GPP and the response to micrometeorology factors of two temperate deciduous broadleaf plantations was explored. The nonlinear relationship of SIF and GPP on the 30 min timescale transformed into a linear correlation with aggregation. With the increase in SIF, GPP gradually saturates and even declines in cork oak. We found SIF “promotion” by using intense incident radiation in cork oak and GPP “depression” or saturation in both species. GPP is more sensitive to instantaneous stresses such as intense radiation imposed high air temperature (Ta) in the midday/afternoon, and the stress threshold of cork oak is lower. We used GPP/SIF as a metric for the relationship, which is significantly higher in poplar, and found GPP/SIF of both species decrease with increasing photosynthetically active radiation (PAR). Since the difference in the amplitude of SIF between tree species was not prominent, the diversity of GPP/SIF performance was mainly caused by the divergence of GPP. The GPP/SIF of cork oak continues to decrease with increasing vapor pressure deficit (VPD), indicating that the relationship between SIF and GPP has a strong daily pattern in the constraint of micrometeorology factors. Our results show that characterizing the impact of micrometeorological conditions on the SIF-GPP relationship in a specific ecosystem is of great significance for the reliable estimation of GPP from remote sensing.

On the Impacts of Historical and Future Climate Changes to the Sustainability of the Main Sardinian Forests

The Mediterranean Basin is affected by climate changes that may have negative effects on forests. This study aimed to evaluate the ability of 17 forests located in the Island of Sardinia to resist or adapt to the past and future climate. Sardinia is experiencing a decreasing anthropic pressure on forests, but drought-triggered dieback in trees was recently observed and confirmed by the analysis of 20 years of satellite tree-cover data (MOD44B). Significant negative trends in yearly tree cover have affected the broad-leaved vegetation, while significative positive trends were found in the bushy sclerophyllous vegetation. Vegetation behavior resulted in being related to the mean annual precipitation (MAP); for MAP &lt; 700 mm, we found a decline in the tall broad-leaved stands and an increase in the short ones, and the opposite was found for bushy sclerophyllous vegetations. In forests with MAP &gt; 700 mm, both stands are stable, regardless of the growing trends in the vapor-pressure deficit (VPD) and temperature. No significative correlation between bushy sclerophyllous tree cover and the climate drivers was found, while broad-leaved tree cover is positively related to MAP1990–2019 and negatively related to the growing annual VPD. We modeled those relationships, and then we used them to coarsely predict the effects of twelve future scenarios (derived from HADGEM2-AO (CMIP5) and HadGEM3-GC31-LL (CMIP6) models) on forest tree covers. All scenarios show an annual VPD increase, and the higher its increase, the higher the trees-cover loss. The future changes in precipitation were contrasting. SC6, in line with past precipitation trends, predicts a further drop in the mean annual precipitation (−7.6%), which would correspond to an average 2.1-times-greater reduction in the tree cover (−16.09%). The future changes in precipitation for CMIP6 scenarios agree on a precipitation reduction in the range of −3.4% (SC7) to −14.29% (S12). However, although the reduction in precipitation predicted in SC12 is almost double that predicted in SC6, the consequent average reduction in TC is comparable and stands at −16%. On the contrary, SC2 predicts a turnaround with an abrupt increase of precipitation (+21.5%) in the upcoming years, with a reduction in the number of forests in water-limited areas and an increase in the percentage of tree cover in almost all forests.

Consistent responses of vegetation gas exchange to elevated atmospheric CO2emerge from heuristic and optimization models

Abstract. Elevated atmospheric CO2 concentration is expected to increase leaf CO2 assimilation rates, thus promoting plant growth and increasing leaf area. It also decreases stomatal conductance, allowing water savings, which have been hypothesized to drive large-scale greening, in particular in arid and semiarid climates. However, the increase in leaf area could reduce the benefits of elevated CO2 concentration through soil water depletion. The net effect of elevated CO2 on leaf- and canopy-level gas exchange remains uncertain. To address this question, we compare the outcomes of a heuristic model based on the Partitioning of Equilibrium Transpiration and Assimilation (PETA) hypothesis and three model variants based on stomatal optimization theory. Predicted relative changes in leaf- and canopy-level gas exchange rates are used as a metric of plant responses to changes in atmospheric CO2 concentration. Both model approaches predict reductions in leaf-level transpiration rate due to decreased stomatal conductance under elevated CO2, but negligible (PETA) or no (optimization) changes in canopy-level transpiration due to the compensatory effect of increased leaf area. Leaf- and canopy-level CO2 assimilation is predicted to increase, with an amplification of the CO2 fertilization effect at the canopy level due to the enhanced leaf area. The expected increase in vapour pressure deficit (VPD) under warmer conditions is generally predicted to decrease the sensitivity of gas exchange to atmospheric CO2 concentration in both models. The consistent predictions by different models that canopy-level transpiration varies little under elevated CO2 due to combined stomatal conductance reduction and leaf area increase highlight the coordination of physiological and morphological characteristics in vegetation to maximize resource use (here water) under altered climatic conditions.

A Novel Method to Simultaneously Measure Leaf Gas Exchange and Water Content

Understanding the relationship between plant water status and productivity and between plant water status and plant mortality is required to effectively quantify and predict the effects of drought on plants. Plant water status is closely linked to leaf water content that may be estimated using remote sensing technologies. Here, we used an inexpensive miniature hyperspectral spectrometer in the 1550–1950 nm wavelength domain to measure changes in silver birch (Betula pendula Roth) leaf water content combined with leaf gas exchange measurements at a sub-minute time resolution, under increasing vapor pressure deficit, CO2 concentrations, and light intensity within the measurement cuvette; we also developed a novel methodology for calibrating reflectance measurements to predict leaf water content for individual leaves. Based on reflectance at 1550 nm, linear regression modeling explained 98–99% of the variation in leaf water content, with a root mean square error of 0.31–0.43 g cm−2. The prediction accuracy of the model represents a c. ten-fold improvement compared to previous studies that have used destructive sampling measurements of several leaves. This novel methodology allows the study of interlinkages between leaf water content, transpiration, and assimilation at a high time resolution that will increase understanding of the movement of water within plants and between plants and the atmosphere.

Coupled Land‐Sea Warming Dominates the Net Land Carbon Uptake Variability in the Greater Bay Area of South China

AbstractAs Earth's warming is driven by anthropogenic activities (e.g., urbanization), land and ocean warming significantly affect the net carbon uptake (NCU) of global ecosystems. However, the driving mechanism of coupled land‐sea warming on the NCU under rapid urbanization in coastal areas remains poorly understood. Here, we used long‐term remote sensing and meteorological observation data combined with spatial analysis and multivariate statistical methods to study the impact of coupled land‐sea warming and urbanization on land net primary productivity (LNPP, a proxy of the net land carbon uptake) in the Guangdong‐Hong Kong‐Macao Greater Bay Area (GBA), a rapidly urbanizing and climatically vulnerable area of South China. We found that the total LNPP decreased by 0.40 TgC from 1985 to 2015 and declined suddenly after 2003, driven by the reduced relative humidity and increased vapor pressure deficit (VPD). The increased VPD was caused by coupled land‐sea warming, and the indirect effect (γ = −0.51, p &lt; 0.001) of coupled land‐sea warming on LNPP variability was much stronger than the direct effect (γ = −0.16, p &lt; 0.05). In addition to the severe decline in the LNPP (0.20 TgC) induced by coupled land‐sea warming, urbanization caused a large loss in LNPP (0.16 TgC), which accounted for 40% of the total LNPP loss. Our findings can support future predictions of regional carbon uptake and inform future climate change mitigation strategies to implement carbon peak and neutrality goals.

Attribution of global evapotranspiration trends based on the Budyko framework

Abstract. Actual evapotranspiration (ET) is an essential variable in the hydrological process, linking carbon, water, and energy cycles. Global ET has significantly changed in the warming climate. Although the increasing vapor pressure deficit (VPD) enhances atmospheric water demand due to global warming, it remains unclear how the dynamics of ET are affected. In this study, using multiple datasets, we disentangled the relative contributions of precipitation, net radiation, air temperature (T1), VPD, and wind speed on the annual ET linear trend using an advanced separation method that considers the Budyko framework. We found that the precipitation variability dominantly controls global ET in the dry climates, while the net radiation has substantial control over ET in the tropical regions, and VPD impacts ET trends in the boreal mid-latitude climate. The critical role of VPD in controlling ET trends is particularly emphasized due to its influence in controlling the carbon–water–energy cycle.

Aerosol Impacts on Water Relations of Camphor (Cinnamomum camphora).

Major parts of anthropogenic and natural aerosols are hygroscopic and deliquesce at high humidity, particularly when depositing to leaf surfaces close to transpiring stomata. Deliquescence and subsequent salt creep may establish thin, extraordinary pathways into the stomata, which foster stomatal uptake of nutrients and water but may also cause stomatal liquid water loss by wicking. Such additional water loss is not accompanied by a wider stomatal aperture with a larger CO2 influx and hypothetically reduces water use efficiency (WUE). Here, the possible direct impacts of aerosols on physical and physiological parameters of camphor (Cinnamomum camphora) were studied (i) in a greenhouse experiment using aerosol exclusion and (ii) in a field study in Taiwan, comparing trees at two sites with different aerosol regimes. Scanning electron microscopy (SEM) images showed that leaves grown under aerosol exclusion in filtered air (FA) were lacking the amorphous, flat areas that were abundant on leaves grown in ambient air (AA), suggesting salt crusts formed from deliquescent aerosols. Increasing vapor pressure deficit (VPD) resulted in half the Ball-Berry slope and double WUE for AA compared to FA leaves. This apparent contradiction to the wicking hypothesis may be due to the independent, overcompensating effect of stomatal closure in response to VPD, which affects AA more than FA stomata. Compared to leaves in a more polluted region in the Taiwanese Southwest, NaCl aerosols dominated the leaf surface conditions on mature camphor trees in Eastern Taiwan, while the considerably lower contact angles and the 2.5 times higher minimum epidermal conductances might have come from organic surfactants. Interpretations of SEM images from leaf surface microstructures should consider amorphous areas as possible indicators of aerosol deposition and other hygroscopic material. The amount and type of the material determine the resulting impacts on plant water relations, together with the surrounding atmosphere and ecophysiological traits.

Tropical tree mortality has increased with rising atmospheric water stress.

Evidence exists that tree mortality is accelerating in some regions of the tropics1,2, with profound consequences for the future of the tropical carbon sink and the global anthropogenic carbon budget left to limit peak global warming below 2 °C. However, the mechanisms that may be driving such mortality changes and whether particular species are especially vulnerable remain unclear3-8. Here we analyse a 49-year record of tree dynamics from 24 old-growth forest plots encompassing a broad climatic gradient across the Australian moist tropics and find that annual tree mortality risk has, on average, doubled across all plots and species over the last 35years, indicating a potential halving in life expectancy and carbon residence time. Associated losses in biomass were not offset by gains from growth and recruitment. Plots in less moist local climates presented higher average mortality risk, but local mean climate did not predict the pace of temporal increase in mortality risk. Species varied in the trajectories of their mortality risk, with the highest average risk found nearer to the upper end of the atmospheric vapour pressure deficit niches of species. A long-term increase in vapour pressure deficit was evident across the region, suggesting that thresholds involving atmospheric water stress, driven by global warming, may be a primary cause of increasing tree mortality in moist tropical forests.

Understanding and Exploiting Transpiration Response to Vapor Pressure Deficit for Water Limited Environments.

More frequent droughts and an increased pressure on water resources, combined with social licence to operate, will inevitably decrease water resources available for fully irrigated cotton production. Therefore, the long-term future of the cotton industry will require more drought tolerant varieties that can perform well when grown in rainfed cropping regions often exposed to intermittent drought. A trait that limits transpiration (TRLim) under an increased vapour pressure deficit (VPD) may increase crop yield in drier atmospheric conditions and potentially conserve soil water to support crop growth later in the growing season. However, this trait has not been tested or identified in cotton production systems. This study tested the hypotheses that (1) genetic variability to the TRLim VPD trait exists amongst 10 genotypes in the Australian cotton breeding programme; (2) genotypes with a TRLim VPD trait use less water in high VPD environments and (3) variation in yield responses of cotton genotypes is linked with the VPD environment and water availability during the peak flowering period. This study combined glasshouse and field experiments to assess plant transpiration and crop yield responses of predominantly locally bred cotton genotypes to a range of atmospheric VPD under Australian climatic conditions. Results indicated that genetic variation to the limiting transpiration VPD trait exists within cotton genotypes in the Australian breeding programme, with five genotypes identified as expressing the TRLim VPD trait. A modelling study suggests that this trait may not necessarily result in overall reduced plant water use due to greater transpiration rates at lower VPD environments negating the water conservation in high VPD environments. However, our study showed that the yield response of cotton genotypes is linked with both VPD environment and water availability during the peak flowering period. Yield performance of the TRLim genotype was improved at some high VPD environments but is unlikely to out-perform a genotype with a lower yield potential. Improved understanding of integrated plant- and crop-level genotypic responses to the VPD environments will enhance germplasm development to benefit cotton production in both rainfed and semi-irrigated cotton systems, thereby meeting the agricultural challenges of the twenty-first Century.

A plasma membrane aquaporin is potentially involved in moderating water stress and photosynthetic depression by sustaining water transport and CO2 uptake under high VPD stress conditions in Solanum lycopersicum (tomato)

The molecular mechanism underlying the plant response to high vapor pressure deficit (VPD) stress remains unclear. In the present study, the roles of the plasma membrane aquaporin SlPIP1;2 in moderating plant water stress and photosynthetic depression in response to a series of VPD stress conditions are investigated. Plant water stress and photosynthetic depression are gradually exacerbated as VPD increases. The effects of SlPIP1;2 in mediating plant water status and photosynthesis are gradually enhanced as VPD increases. SlPIP1;2 exerts no significant effect on plant water status and photosynthesis under low VPD conditions. Overexpression of SlPIP1;2 moderates plant water stress by enhancing root water uptake under high VPD stress conditions, sustaining stomatal and mesophyll conductance for photosynthetic CO2 uptake and transport. Consequently, the relatively high CO2 availability inside chloroplasts in SlPIP1;2-overexpressing plants efficiently moderates photosynthetic depression under high VPD stress conditions. The present study demonstrates that SlPIP1;2 is potentially involved in moderating water stress and photosynthetic depression by sustaining water transport and CO2 uptake under high VPD stress conditions.

Plant hydraulics, stomatal control, and the response of a tropical forest to water stress over multiple temporal scales.

Many tropical regions are experiencing an intensification of drought, with increasing severity and frequency. The ecosystem response to these changes is still highly uncertain. On short time scales (from diurnal to seasonal), tropical forests respond to water stress by physiological controls, such as stomatal regulation and phenological adjustment, to cope with increasing atmospheric water demand and reduced water supply. However, the interactions among biological processes and co-varying environmental factors that determine the ecosystem-level fluxes are still unclear. Furthermore, climate variability at longer time scales, such as that generated by ENSO, produces less predictable effects because it depends on a highly stochastic combination of factors that might vary among forests and even between events in the same forest. This study will present some emerging patterns of response to water stress from 5years of water, carbon, and energy fluxes observed on a seasonal tropical forest in central Panama, including an increase in productivity during the 2015 El Niño. These responses depend on the combination of environmental factors experienced by the forest throughout the seasonal cycle, in particular, increase in solar radiation, stimulating productivity, and increasing vapor pressure deficit (VPD) and decreasing soil moisture, limiting stomata opening. These results suggest a critical role of plant hydraulics in mediating the response to water stress over a broad range of temporal scales (diurnal, intraseasonal, seasonal, and interannual), by acclimating canopy conductance to light and VPD during different soil moisture regimes. A multilayer photosynthesis model coupled with a plant hydraulics scheme can reproduce these complex responses. However, results depend critically on parameters regulating water transport efficiency and the cost of water stress. As these costs have not been properly identified and quantified yet, more empirical research is needed to elucidate physiological mechanisms of hydraulic failure and recover, for example embolism repair and xylem regrowth.

Observations of aerosol–vapor pressure deficit–evaporative fraction coupling over India

Abstract. Northern India is a densely populated subtropical region with heavy aerosol loading (mean aerosol optical depth or AOD is ∼0.7), frequent heat waves, and strong atmosphere–biosphere coupling, making it ideal for studying the impacts of aerosols and the temperature variation in latent heat flux (LH) and evaporative fraction (EF). Here, using in situ observations during the onset of the summer monsoon over a semi-natural grassland site in this region, we confirm that strong co-variability exists among aerosols, LH, air temperature (Tair), and the vapor pressure deficit (VPD). Since the surface evapotranspiration is strongly controlled by both physical (available energy and moisture demand) and physiological (canopy and aerodynamic resistance) factors, we separately analyze our data for different combinations of aerosols and Tair/VPD changes. We find that aerosol loading and warmer conditions both reduce sensible heat (SH). Furthermore, we find that an increase in atmospheric VPD tends to decrease the gross primary production (GPP) and, thus, LH, most likely as a response to stomatal closure of the dominant grasses at this location. In contrast, under heavy aerosol loading, LH is enhanced partly due to the physiological control exerted by the diffuse radiation fertilization effect (thus increasing EF). Moreover, LH and EF increases with aerosol loading even under heat wave conditions, indicating a decoupling of the plant's response to the VPD enhancement (stomatal closure) in the presence of high aerosol conditions. Our results encourage detailed in situ experiments and mechanistic modeling of AOD–VPD–EF coupling for a better understanding of Indian monsoon dynamics and crop vulnerability in a heat stressed and heavily polluted future India.