Increase In Oil Yield Research Articles

Effect of superheated steam treatment on yield, physicochemical properties and volatile profiles of perilla seed oil

This study investigated the effect of superheated steam (SHS) treatment on the yield and quality properties, including volatile compounds of perilla seeds oil (PO). SHS treatment resulted in ruptured seed coat and damaged cellular structure, increasing oil yield (78.81 g/100 g seeds), about 2.5-times higher yield than the untreated seeds (33.11 g/100 g seeds). The viscosity (~89 mPa s), color parameters (L*a*b*), acid value (0.19 mg KOH/g), and peroxide value (1.02 meq/kg) of SHS-treated POs were similar with that of the untreated PO, indicating no oil rancidity by SHS treatment. SHS-treated PO showed a dramatic reduction of lipase activity from 37.44 to 0.80 μmol/g, 3-times higher phenolic content and 5-times higher antioxidant activity than that of the untreated PO. The major volatiles were 2,4-heptadienal, 4-methyl-5-(2-methyl-2-propenyl-2(5H)-furanone, 3-(4-methyl-3-pentenyl)furan perillen, and 3-methylpentane in POs. The normalized relative intensities of the volatiles were reduced for SHS-treated PO. Thus, SHS could be an effective treatment for high oil yield without originating odor characteristics compounds in PO.

Chicken Manure and Phosphorus Influence on Biomass Production and Chemical Composition of the Essential Oil of Ocimum kilimandscharicum

The effects of soil incorporation of five rates of semi-decomposed chicken manure (0, 5, 10, 15 and 20 t ha-1), with and without the addition of phosphorus (200 kg P2O5 ha-1) on biomass production and chemical composition of the essential oil from leaves of African blue basil were evaluated. The experimental design was a randomized complete block design in a 5 × 2 factorial scheme, with four replicates. The first cut of plants was performed at 70 days after transplanting (DAT) and the second at 140 DAT. The addition of 20 t ha-1 of chicken manure to the soil induced increase in plants height, fresh and dry mass production and yield of essential oil. The use of chicken manure induced an increase in camphor content and decreased content of 1,8 cineole. After regrowth, biomass production of African blue basil was higher when compared to the first cut.

Production of algal oil from power plant algae

ABSTRACT Algae growing in the thermal power plant environment are considered as waste for the power industry. But the algal biomass could act as efficient CO2-bio-sequester as they are adapted to grow under high CO2 concentration and the algal oil extracted from those algae is a cheap source of bio-diesel or green energy, thus mitigating the environmental pollution and conversion of ‘waste-to-wealth’. The present study aims at the production of bio-oil from a macro-alga, Pithophora varia collected and identified from water bodies of a thermal-power-plant in India. The oil productivity of the alga can be correlated with the biomass growth. The alga was cultivated in Erlenmeyer-flasks with external CO2 supplementation of 0.15 mole fraction in air. The light intensity 10,897 lux was incident on the flask’s wall when placed under sunlight for 8 h. Simultaneously, oil yield of 18.7% has been measured before subjecting the alga to the growth and starvation phase. An increase in oil-yield was measured on day 9 for both growth (26.2%) and starvation phases (29.3%). During growth phase, initiation of conversion of reserve food materials to lipid granules advocates for the oil yield. But the increment in lipid productivity during the starvation phase might have resulted due to nutrient deficiency and initiation of the stimulation of lipid-synthetic pathways towards the formation of triacylglceride.

Effect of Potash addition on groundnut oil yield and quality of <i>kulikuli </i>(fried groundnut cake)

Fried groundnut cake, popularly called kulikuli, is obtained by frying the cake obtained from groundnut oil extraction. Addition of potash during defatting of groundnut is believed to increase oil yield and improve kulikuli quality, but there is little or no scientific information explaining this. This study investigated the yield, physical and chemical properties of oil extracted from groundnut with the addition of potash at 0.16, 0.32, 0.48 and 0.64%. The proximate and sensory attributes of the kulikuli produced were also determined. Groundnut yield increased from 14% to 27% with increasing addition of potash, presumably due to the emulsifying properties of potash. Potash addition significantly (p < 0.05) influenced the refractive indices, percentage impurity and colour of the oil samples. Oil extracted without potash had the lowest iodine value (85.12 mg/100 g), saponification value (202.2 mg KOH/g), acid value (6.46 mgKOH/g) and peroxide value (5.66 meq/Kg). The kulikuli had moisture in the range of 1.45 - 3.00%; carbohydrate, 20.6 - 40.4%; protein, 30.5 - 40.8%; ash, 4.45 - 5.05%; and fat, 23.5 - 30.6%. Kulikuli sample without potash was the most preferred based on taste and aroma while sample with 0.64% potash was the most preferred based on crunchiness, colour, breakability and overall acceptability. The study confirmed the hypothesis that potash addition during extraction of oil and production of kulikuli increases oil yield and some of the quality attributes of the kulikuli.
 Keywords: Groundnut, potash, oil yield, kulikuli, quality attributes

Effects of salicylic acid on growth, mineral nutrition, glandular hairs distribution and essential oil composition in Salvia officinalis L. grown under copper stress

Higher uptake and translocation of copper (Cu) into plant tissues can cause serious physiological and biochemical alterations. Salicylic acid (SA) is a well-known naturally occurring signaling molecule responsible for inducing environmental stress tolerance in plants. Therefore, spraying of SA could provide protection against several types of stresses such as Cu toxicity. In this sense, a pot study was conducted to find out whether spraying of SA (0.5 and 1 mM) could ameliorate the adverse effect of Cu toxicity (40 mM) in Salvia officinalis L. plants. The results showed that the Cu stress significantly decreased the stem and root growth, and it reduced the calcium (Ca), phosphorus (P) and potasium (K) content of leaves and roots. Besides, Cu stress increased essential oil yield by 16.66% as compared to control. On the other hand, SA spraying on stressed plants significantly improved stem and root growth and increased Ca, P and K content. This improvement was more pronounced at 0.5 mM SA concentration. The essential oil content also increased under treatment with SA, specially at 0.5 mM concentration, with 116.66% enhancement as compared to stressed plants (untreated with SA). The increase in essential oil yield under all treatments was associated with significant enhancement in the density of peltate glands, with a much higher density when SA was applied at 0.5 mM concentration in presence of Cu stress. The chemical composition of oil changed according to the treatment. Cu stress led to a reduction of 19% in oxygenated monoterpenes levels, mainly due to the decrease in the major compounds, α-thujone, camphor and 1.8-cineol. However, spraying SA on stressed plants led to the recovery of normal levels of these compounds.

Effect of Blanching Pomegranate Seeds on Physicochemical Attributes, Bioactive Compounds and Antioxidant Activity of Extracted Oil.

This study investigated the effect of blanching pomegranate seeds (PS) on oil yield, refractive index (RI), yellowness index (YI), conjugated dienes (K232), conjugated trienes (K270), total carotenoid content (TCC), total phenolic compounds (TPC) and DPPH radical scavenging of the extracted oil. Furthermore, phytosterol and fatty acid compositions of the oil extracted under optimum blanching conditions were compared with those from the oil extracted from unblanched PS. Three different blanching temperature levels (80, 90, and 100 °C) were studied at a constant blanching time of 3 min. The blanching time was then increased to 5 min at the established optimum blanching temperature (90 °C). Blanching PS increased oil yield, K232, K270, stigmasterol, punicic acid, TPC and DPPH radical scavenging, whereas YI, β-sitosterol, palmitic acid and linoleic acid were decreased. The RI, TCC, brassicasterol, stearic acid, oleic acid and arachidic acid of the extracted oil were not significantly (p > 0.05) affected by blanching. Blanching PS at 90 °C for 3 to 5 min was associated with oil yield, TPC and DPPH. Blanching PS at 90 °C for 3 to 5 min will not only increase oil yield but could also improve functional properties such as antioxidant activity, which are desirable in the cosmetic, pharmaceutical, nutraceutical and food industries.

Microwave pre-treatment of canola seeds and flaked seeds for increased hot expeller oil yield.

Microwave (MW) pre-treatment of canola seeds or flaked seeds was found to be a superior alternative to the conventional thermal pre-treatment (steam). Flaked seeds were "cooked" (heat-treated) with steam or using microwave treatments in the temperature range of 62-130°C prior to expeller pressing. Microwave cooking at 100°C resulted in the highest increase in the pressed oil yield, which is an increase of 3.7% (w/w) on a pressed oil basis or 9.0% (oil in seed basis) compared with steam cooking. Whole canola seeds conditioning was conducted with microwaves or steam, in the temperature range of 40-75°C, followed by microwave or steam cooking at 100°C to evaluate the effect of MW treatment during conditioning on the expeller oil yield. The use of a continuous microwave process for combined conditioning of whole seeds at 55°C and subsequent cooking of flaked seeds at 100°C resulted in a 4.0% increase in expeller oil yield, compared with steam conditioningand cooking. The influence of dry basis (db %) moisture contents of 5%, 11.5%, and 16.5% on oil yield after steam or MW treatments of seeds and flaked seeds was also studied. The moisture content of 11.5% (db %) yielded the highest net oil yield for both MW and steam at best conditioning and cooking temperatures of 55°C and 100°C, respectively. No significant impact of MW cooking was seen on oil quality compared with conventional steam cooking.

Variation of essential oil composition of fruits of <i>Zanthoxylum leprieurii</i> Guill. et Perr (Rutaceae) from different sample locations and during their maturation period

The fruits of Zanthoxylum leprieurii Guill. & Perr. (Rutaceae) are traditionally used in Africa, particularly in Cameroon, as a spice and in the treatment of sickle cell anaemia. The objective of this study was to investigate the fruit essential oils from different sample locations and during their maturation period by using GC and GC-MS. Except for one sample collected in Aniak which consisted mainly of oxygenated monoterpenes, the volatile composition of Z. leprieurii fruits exhibited relative high amounts of monoterpene hydrocarbons (61.1-90.9%) such as (E)-β-ocimene (13.4-75.2%) and a-pinene (1.1-40.7%). The influence of the maturity stage on the chemical composition of the essential oils has resulted in an increase of oil yield and variations in the content of volatile compounds (a-pinene 13.3-75.6; (E)-β-ocimene 2.9-64.0%).
 Keywords: Senegal, Zanthoxylum leprieurii fruits, essential oil, chemical variability, stage of maturity and GC-MS.

Roles of Co-solvents in hydrothermal liquefaction of low-lipid, high-protein algae

Valorization of algal biomass is often limited by its low lipid content. Here, different alcohols: ethanol, isopropanol, and glycerol, were studied as co-solvents to improve the conversion efficiency of a lipid-poor microalgae, Galdieria sulphuraria, by hydrothermal liquefaction. Bio-crude oil yield increases, from 13 to 73 wt% (on dry algae basis), were attributed to the alcohols facilitating the transfer of algal protein-derived fragments from the aqueous phase into the oil phase. A series of characterization results showed that bio-crude oil formation was mainly the result of alcohols reacting with algal fragments via Maillard reactions, alkylation, and esterification, respectively. Insights into the synergistic effect of low-lipid feed and alcohol provide mechanistic support for choosing an alcohol-rich waste, crude glycerol, to improve bio-crude oil production from HTL of wastewater-grown G. sulphuraria. Promising improvements in yield and energy recovery indicates competitive economics for a low-lipid biomass waste-to-biofuel conversion technique.

Plant Unsaturated Fatty Acids: Biosynthesis and Regulation.

In most plants, major unsaturated fatty acids (UFAs) are three C18 species, namely, oleic (18:1), linoleic (18:2), and α-linolenic (18:3) acids. These simple compounds play multiple crucial roles in planta and are also important economic traits of oil crops. The enzymatic steps of C18 UFA biosynthesis have been well established. However, the associated FA/lipid trafficking between the plastid and the endoplasmic reticulum remains largely unclear, as does the regulation of the expression and activities of the involved enzymes. In this review, we will revisit the biosynthesis of C18 UFAs with an emphasis on the trafficking, and present an overview of the key enzymes and their regulation. Of particular interest is the emerging regulatory network composed of transcriptional factors and upstream signaling pathways. The review thereby provides the promise of using physical, biochemical and/or genetic means to manipulate FA composition and increase oil yield in crop improvement.

Improved canola oil expeller extraction using a pilot-scale continuous flow microwave system for pre-treatment of seeds and flaked seeds

Continuous flow microwave (MW) pre-treatment of canola seeds and flaked canola seeds increases the canola oil extraction yield on the expeller compared with conventional thermal pre-treatment using steam heating. Flaked seeds were “cooked” (heat-treated) with steam or using microwave heating in the temperature range of 70 °C–110 °C before expeller pressing. Microwave cooking at 100 °C resulted in the highest increase in the pressed oil yield which is an increase of 4.2% (w/w) on a pressed oil basis or 9.2% (oil in seed basis) compared with steam cooking. Whole canola seeds conditioning was conducted with microwaves or steam, in the temperature range of 40 °C–60 °C, followed by microwave or steam cooking at 100 °C to evaluate the effects on the expeller oil yield. The use of a continuous microwave process for combined conditioning of whole seeds at 50 °C and subsequent cooking of flaked seeds at 100 °C gave a 4.3% increase in expeller oil yield, compared with steam conditioning and cooking at the same temperature. The effects of steam and MW treatments on the dielectric properties of flaked seeds (dielectric constant, loss factor and loss tangent) were investigated at a microwave frequency of 2450 MHz at three temperatures (70, 90, and 100 °C). The results showed that the dielectric properties of MW treated flaked seeds were higher than those of samples treated with steam. The lipid classes of the extracted oil which included triacylglycerols (TAG), diacylglycerols (DAG), free fatty acids (FFA), and phospholipids (PL) and their fatty acid compositions from both the steam and continuous microwave processes were analysed. The contents of polyunsaturated fatty acids (PUFA), monounsaturated fatty acids (MUFA) and saturated fatty acids (SFA) after control and continuous MW cooking were not significantly different, nor was there any significant difference in the oil quality parameters such as FFA content, peroxide value (PV), phosphorus and chlorophyll contents between MW and conventional steam heating.

Ultrasound, a powerful non-thermal and green technique on phenolic fractional profiles (free, conjugated glycoside, conjugate esters and bound) in fermented seabuckthorn beverage

Phenolic compounds were characterized after traditional extraction method (TEM) and ultrasound-assisted extractions (UAEs) for 10 min (US10) and 15 min (US15). Four fractions (free, bound, conjugated esters, and conjugated glycosides) were obtained and characterized using RP-HPLC-PDA.
 The US10 extracted 22.1 % (454.3 mg/kg) phenolic compounds while US15 extracted 66.6% (1369.7 mg/kg) phenolic compounds compared to traditional extraction method (TEM) after 1 hour of extraction. US10 and TEM extracted a similar amount of aglycones (112.3 + 4.0 and 109.0 + 12.9 mg/kg, respectively) while US15 showed a significant decrease in aglycone extraction (58.9 + 4.1 mg/kg) (P<0.05). 
 In seabuckthorn kombucha, US decreased initial microbial load by 2.6 log CFU/mL, increased ORAC value by 3% and increased water solubility index (WSI) by 40% (from 6.64 g/g to 9.29 g/g) without syneresis. Results from this study suggest that application of US can enhance phenolic functionality during fermentation and is capable of decreasing syneresis, increasing oil yield, decreasing microbial load, and increasing ORAC with minimal loss of nutritional quality.

Extraction of Calophyllum oil as biodiesel raw material using a binary solvent

The utilization of renewable raw material, low-cost feedstock, and efficient energy are the main parameters in sustainable biodiesel production. Calophyllum seeds are potential as a vegetable oil source for biodiesel production due to their high oil content and productivity. Calophyllum oil extraction is usually conducted by the mechanical press, but the yield and quality of oil produced by this method are very low. Calophyllum oil extraction using a binary solvent (a mixture of n-hexane and alcohol) was thus investigated to meet the aspects of sustainability and to solve the problem of mechanical extraction. The influence of extraction condition was examined to determine optimal oil yield and quality. The mixture of n-hexane and alcohol effectively extracts oil from Calophyllum seeds and simultaneously purified it from resin. The n-Hexane-to-alcohol ratio had a significant effect on oil recovery and its quality. The augmentation of the n-hexane-to-alcohol ratio from 1:1 to 2.5:1 increased oil yield. Acid value and density of oil improved as the n-hexane-to-alcohol ratio declined from 2.5:1 to 1:1. The n-Hexane-to-alcohol ratio of 2.5:1 has provided the best yield (88.0%) of oil extract at 40°C for 5 h. The oil present its best quality at 0.893 g/cm3 of density, 41.0 mPa.s of viscosity, 8.8 mg KOH/g of acid value, 88.3 g/100 g of iodine value, < 1% of moisture content and < 0.04% of ash content.

SIMULATION OF CO-PROCESSING BIO-OIL AND VGO IN FLUID CATALYTIC CRACKING UNITS

Biofuel is a promising substitute for fossil fuels to reduce greenhouse gas emissions and to provide highly sustainable fuels. Several technical challenges are indeed present during upgrading bio-oil to transportation fuel on a large scale. Co-processing bio-oil with some petroleum fractions in existing refineries serves as an alternative method to minimise processing costs. This paper aims to evaluate the co-processing by exploring the effects of temperature, bio-oil ratios and types of bio-oil to the product yields and quality in a Fluid Catalytic Cracking (FCC) unit within a refinery complex. The considered bio-oil are produced from pyrolysis of Palm Kernel Shell (PKS) and Empty Fruit bunch (EFB). The results show that bio-oil from PKS is better suited to produce gasoline due to its aromatic nature and its carbon range similarities compared to that from EFB. A mixture of 20% of hydrodeoxygenated (HDO) PKS in vacuum gas oil (VGO) shows a 5% improvement of naphtha yield while 20% raw bio-oil from PKS produces 4% increase in light cycle oil (LCO) yield.&#x0D; Keywords: co-processing, fluid catalytic cracking, bio-oil, palm kernel shell, empty fruit bunch, simulation

Change in some quality parameters and oxidative stability of olive oils with regard to ultrasound pretreatment, depitting and water addition

Ultrasound pretreatment with different times (0, 4, 8, 10 min) on olive paste previous malaxation of olive oil extraction along with depitting and water application procedures were studied. The effects of these procedures on oil yield, quality parameters and oxidative stability have been studied. Therefore, the olive oil yield increase with increasing time of ultrasound treatment. On the other hand, the application of ultrasound did not adversely affect the quality characteristics, as well as the antioxidant activity, when comparing with untreated paste. Furthermore, the oxidative stability data, we can conclude that ultrasound treatment can affect the olive oil oxidative stability. This study could provide useful information for industry to produce olive oil with high yield and quality.

Two Plastid Fatty Acid Exporters Contribute to Seed Oil Accumulation in Arabidopsis.

Triacylglycerols (TAGs) are the major storage form of seed oil in oilseed plants. They are biosynthesized de novo in seed plastids and then transported into the endoplasmic reticulum. However, the transport mechanism for plastid fatty acids in developing seeds remains unknown. Here, we isolated two novel plastid fatty acid exporters (FATTYACID EXPORT 2 [FAX2] and FAX4, respectively) specifically abundant in seed embryos during the seed-filling stage in Arabidopsis (Arabidopsis thaliana). FAX2 and FAX4 were both localized to the chloroplast membrane. FAX2 and FAX4 loss-of-function mutations caused deficiencies in embryo and cotyledon development. Seeds of fax2fax4 double mutants exhibited significantly reduced TAG contents but elevated levels of plastid lipid contents compared with those of wild-type plants. By contrast, overexpression of FAX2 or FAX4 enhanced TAG deposition. Seed-feeding experiments showed that the two FAX proteins transported 14C-plastid fatty acids and 13C-oleic acids for TAG biosynthesis during the seed-filling stage. Together, our data demonstrate that FAX2 and FAX4 play critical roles in transporting plastid fatty acids for TAG biosynthesis during seed embryo development. These two transporters may have broad application for increasing oil yield in oilseed crops.

Introducing genetically improved Melaleuca cajuputi subsp cajuputi to increase farmers’s welfare: a success story in Papua

Cajuput tree is native to Maluku and produce 1,8 cineole based essential oil. Since 1995, the Centre of Biotechnology and Tree Improvement has implemented a genetic improvement program of cajuput aiming to increase oil yield and oil content. The program has successfully produced superior seeds with the yield of 2%. In early 2016, a small scale pilot plantation of cajuput was established in 5 ha community land di Kampung Rimbajaya Distrik Biak Timur Kabupaten Biak, Papua. Leaf distillation started at the end of 2017 using a simple distillation unit. Previously, the local farmers were woodcutters and shifting cultivators. After the establishment of cajuput plantation, they became permanent farmers and cajuput oil refiners. Leaf distillation of genetically improved trees produced oil yield of 1.3%, a significant improvement from the average of 0.6 – 0.8% yield of unimproved trees. This 5 ha plantation of 2500 trees/ha potentially produce 400 kg/year. Some of the cajuput oil is sold in a local market in 8ml bottle. This cajuput oil production scheme has increased the farmers welfare. Furthermore, the success story of Kovarwis farmers inspired other farmers surrounding Rimbajaya to develop similar plantation. When this small-scale plantation is replicated in other parts of Indonesia, cajuput oil production will increase and Indonesia may one day become self-sufficient in cajuput oil. An agreement with cajuput packaging company is being establish to ensure that the oil produce by farmers will be purchased by the company and thus ensuring steady supply of good quality oil.

CURRENT TRENDS, METHODS AND RESULTS OF BROWN MUSTARD (BRASSICA JUNCEA) AND BLACK MUSTARD (BRASSICA NÍGRA) BREEDING

Background. Various types of mustard and its forms differ significantly from each other in a set of morphological, biological, biochemical and other economically important traits. Both brown and black mustard are in great demand.Materials and methods. The study was conducted in 2009– 2018 on the brown mustard cultivar ‘Rosinka’ using the VNIIMK techniques. Promising cultivars of brown mustard (‘Zolushka’, ‘Nika’, ‘Yunona’) and black mustard (‘Niagara’) as well as source material with good breeding prospects were evaluated for their economic significance.Results and discussion. Individual selection based on self-pollination in the seed production of brown mustard population cultivars was found to facilitate identification of high-yielding lines, promptly increase the yield of cultivars, isolate lines exceeding the initial population in oil yield, and obtain biotypes with unusual fatty acid composition. The best accessions demonstrated an increase of 1.0–2.9% in their oil content. Besides, the selected material had the yield at the same or higher level than the reference (on average, 3.22 t/ha). An increase of oil yield varied from 0.23 to 0.36 t/ha. The technique of recurrent individual selection of elite plants from interspecific hybrid populations with the use of selfpollination resulted in developing 3 brown mustard cultivars: ‘Zolushka’, ‘Nika’ and ‘Yunona’. One of the important trends in mustard breeding is the development of cultivars with a high content of allyl mustard oil. As a result of this work, a new brown mustard cultivar was developed (‘Gorlinka’). One of the representatives of the economically important Brassicaceae family is the black mustard. Its new cultivar ‘Niagara’ was developed by recurrent individual selection from accession k-2632 preserved in the collection of the Vavilov Institute of Plant Genetic Resources (VIR). In competitive trials ‘Niagara’ demonstrated an average yield at the level of 1.5–1.7 t/ha, and the oil content in seeds of 37.0– 40.0%. Cv. ‘Niagara’ has a short growing season.

Economic valuable traits of promising breeding samples and ‘Chornolysta’ variety of Mentha piperita L. after in vitro sanitation and micropropagation

Purpose. To study the impact of clonal micropropagation and sanitation in vitro by viricide Ribavirin on ex vitro plant productivity, quantitative content and qualitative composition of peppermint essential oil components obtained from four breeding samples of peppermint plants (Mentha piperita L.) and the ‘Chornolysta’ variety. Methods . The study used methods of field agrotechnical one-factor experiment, essential oil distillation with water vapor according to the Ginzberg method, capillary gas chromatography and statistical analysis. Results. Due to the sanitation process in vitro, the yield of air-dried leaves of breeding samples increased by 2.9–7.1%, the ‘Chornolysta’ variety by 51.4% and rhizomes by 2.2–3.8% and 28.5% respectively, which amounted 0.35–2.74 t/ha. A significant increase of essential oil yield of breeding samples from 4.0 to 9.9 kg/ha was shown, and of the ‘Chornolysta’ variety – up to 28.6 kg/ha. After in vitro sanitation and clonal cropropagation of the breeding sample M 01-12, the content of essential oil was more than 4%. The following components of peppermint essential oil were identified: limonene, cineole, menton, mentofuran, isomenton, menthyl acetate, β-caryophyllene, isomenthol, menthol, pulegon, piperitone and carvone. A clear tendency to a decrease in the amount of menton and isomenthol with isomenton and menthol increase in plants, sanitated and propagated in vitro, was revealed. Conclusions . The use of tissue and organ culture methods and in vitro sanitation improves the qualitative composition of terpenoids by increasing the amount of menthol and menton reducing. The data obtained on the composition of terpenoids should be considered in peppermint selection as one of the integral criteria, which should be included in the list of economically valuable characteristics of peppermint plants, such as foliage, biomass of air-dried leaves, plant rhizomes and the amount of peppermint essential oil. Six indicators of the essential oil of the breeding sample M 01-02, namely citric acid, cineole, mentofuran, isomentone, pulegon, carvone, as well as the cineole / limonene ratio, meet the criteria of the European Pharmacopoeia, so it can be considered as promising for cultivation among the studied samples.

Catalytic pyrolysis of chemical extraction residue from microalgae biomass

Pyrolysis characteristics of chemical extraction microalgae residue in the presence and absence of catalysts (KCl, KOH, K2CO3, Al2O3, CaO, MgO and pyrolysis char of microalgae residue) were evaluated using a thermal analyzer and a tube furnace reactor. These catalysts can effectively improve the oil yield during microalgae residue pyrolysis. The order of the strength on increasing the oil yield was: pyrolysis char of microalgae residue > Al2O3>CaO > K2CO3>MgO > KCl > KOH. The addition of all the catalysts can effectively reduce the potential ecological risk of heavy metals in pyrolysis chars. The addition of KCl, MgO and Al2O3 can increase the content of gasoline components in pyrolysis oil. All the catalysts can reduce the content of CO2 in the pyrolysis gas, and increase the content of CO and hydrocarbon gases. KCl addition showed considerable increase in both yield and quality of pyrolysis oil and gas with a proportion of 5 wt % during microalgae residue pyrolysis.