Increase In Total Protein Content Research Articles

Extending the G1 phase improves the production of lipophilic compounds in yeast by boosting enzyme expression and increasing cell size

Cell phase engineering can significantly impact protein synthesis and cell size, potentially enhancing the production of lipophilic products. This study investigated the impact of G1 phase extension on resource allocation, metabolic functions, and the unfolded protein response (UPR) in yeast, along with the potential for enhancing the production of lipophilic compounds. In brief, the regulation of the G1 phase was achieved by deleting CLN3 (G1 cyclin) in various yeast strains. This modification resulted in a 83% increase in cell volume, a 76.9% increase in dry cell weight, a 82% increase in total protein content, a 41% increase in carotenoid production, and a 159% increase in fatty alcohol production. Transcriptomic analysis revealed significant upregulation of multiple metabolic pathways involved in acetyl-CoA (acetyl coenzyme A) synthesis, ensuring an ample supply of precursors for the synthesis of lipophilic products. Furthermore, we observed improved protein synthesis, attributed to UPR activation during the prolonged G1 phase. These findings not only enhanced our understanding and application of yeast's capacity to synthesize lipophilic compounds in applied biotechnology but also offered unique insights into cellular behavior during the modified G1 phase, particularly regarding the UPR response, for basic research. This study demonstrates the potential of G1 phase intervention to increase the yield of hydrophobic compounds in yeast, providing a promising direction for further research.

Development of High-Production Bacterial Biomimetic Vesicles for Inducing Mucosal Immunity Against Avian Pathogenic Escherichia coli

To evaluate the immunoprotective effect of bacterial biomimetic vesicles (BBVs) against avian pathogenic Escherichia coli (APEC), a ΔtolA J11 mutant strain was generated by deleting the tolA gene in the low pathogenic O78 serotype J11 strain. The total protein content of outer membrane vesicles (OMVs) derived from the ΔtolA J11 strain exhibited a sevenfold increase compared to the wild-type strain. Additionally, high-pressure homogenization technology was employed to produce BBVs, resulting in a sixfold increase in total protein content compared to spontaneously secreted OMVs from ΔtolA J11. The immunogenicity of both OMVs and BBVs was assessed through intranasal or intramuscular immunization in specific pathogen-free (SPF) chickens. Results demonstrated that intranasal immunization with OMVs or BBVs in chickens elicited specific IgY antibodies against APEC outer membrane proteins and specific sIgA antibodies in the nasal cavity and trachea, as well as a significant increase in the proliferation response of chicken peripheral blood lymphocytes. The bacterial load in the blood and various organs of the challenged chickens were significantly reduced, resulting in a 66.67% and 58.30% survival rate against a high pathogenic serotype O78 strain challenge, while the control group exhibited only a 16.67% survival rate. The intramuscular immunization with OMVs or BBVs in chickens only induced specific IgY antibodies, with a survival rate of only 33.33% for challenged chickens during the same period. Therefore, intranasal vaccination of the highly productive BBVs is capable of eliciting an immune response similar to that of OMVs and providing protection against APEC infection, thus offering innovative insights for the advancement of APEC vaccines.

Functional and sensory evaluation of bread made from wheat flour fortified with wine byproducts

Grape pomace is the main byproduct of the wine industry and an important source of dietary fiber and phenolic compounds. Grape pomace powder (GPP) partially substituted 8, 10, 12, 15, and 25% of the wheat flour in bread formulations. The proximate composition, total dietary fiber content, phenolic compounds, texture profile, color, and bioaccessibility of phenolic compounds in vitro were measured in the bread. Bread sensory acceptance by consumers was determined using a 9-point hedonic scale. Compared with the control bread (CB), the 8% GPB-substituted bread presented the best results and exhibited an increase in total protein content (7.5%) and total dietary fiber content (6.1%). The total phenolic content was greater in GPB (5.1 mg GAE/g) than in CB (2.1 mg GAE/g). Adding GPP to the bread affected the color, and the color of the GPB-treated bread was darker than that of the CB-treated bread. Still, no significant differences were detected regarding the texture profile or consumer sensory acceptance between the GPB-treated and CB-treated bread. The in vitro analysis of phenolic compound bioaccessibility revealed no differences between the two samples during gastrointestinal digestion. GPP is an interesting byproduct that can be used in bakery. The replacement of 8% of the bread with GPP increased the nutritional content of the bread, particularly the protein, total dietary fiber, and total phenolic content, without affecting the texture or sensory acceptance of the bread. To understand the possible beneficial effect of GPB on consumers, further research on the bioavailability of phenolic compounds and the impact of dietary fiber increment needs to be assessed.Graphical

The effect of nitrogen management on seed yield and quality in traditional and canola-quality white mustard

The article presents the results of a three-year field study that was conducted in Poland to evaluate the yield and quality of seeds and oil from traditional (SAM) and canola-quality white mustard (SAC) in response to different N fertilizer rates (0, 40, 80, 120, and 160 kg ha–1). Seed yields were 25% higher in SAM than SAC. The seeds of SAC contained more crude fat (by 3%) and crude fiber (by 6%) than the seeds of SAM. In turn, the seeds of SAM were a richer source of total protein (by 7%). The content of glucosinolates (GSLs) was 8–10 times lower in the seeds of SAC than SAM. The seeds of both mustard cultivars were most abundant in γ-tocopherol (γ-T) (90–94%). The seeds of SAC were characterized by a higher content of γ-T and a lower α-T/γ-T ratio than SAM seeds. White mustard oil contained mostly MUFAs (69–75%). However, C22:1 accounted for more than 50% of MUFAs in the oil SAM. In the oil SAC, the proportion of C22:1 did not exceed 6%, whereas C18:1 accounted for nearly 85% of total MUFAs. Nitrogen fertilization induced a significant increase in seed yields (by 33%), a decrease in crude fat content (by 3–4%), and an increase in total protein content (by 4%), and crude fiber content (by 7%). Nitrogen decreased GSL levels by 31% in SAM seeds. In SAC, N fertilization induced differences in the qualitative composition of GSLs, but did not affect the total GSL content of seeds. Higher N rates increased the content of α-T and γ-T, the α-T/γ-T ratio, and total T content. Nitrogen fertilization decreased the content of C18:3 in the seeds of the SAM. In SAC, the application of N decreased the content of C18, C18:1, and C18:3, and increased the biosynthesis of C18:2, C20:1, and C22:1.

Stress biomarker response in Aporrectodea caliginosa earthworms exposed to single and combined pesticide treatments (Prosaro and Decis).

This study aims to assess the impact of two pesticides commonly used in Algeria (Prosaro XRT and Decis 25 EC), as well as their combinations at recommended doses, on a non-target species bioindicator of soil pollution, the earthworm Aporrectodea caliginosa, using physiological (mortality and growth) and biochemical parameters (proteins, glutathione, catalase activity and glutathione S-transferase, acetylcholine esterase, lipoxygenase). The recommended dose and its double were tested individually and in combination for this. It should be noted that the protocol used and the initial concentrations selected are the same as those used in the field. After 7 and 14 days (7D/14D) of exposure, all dosages were administered. Our findings show that the pesticides tested had no effect on earthworm survival. However, a significant decrease in their growth rates depending on the different concentrations was observed for the different treatments over the entire exposure period of 7 or 14 D. The greatest reductions (31.62%, 35.04%) are reported after 14D for the high concentrations of Decis alone (D2) as well as for the combined treatment Prosaro/Decis (P2/D2). At the same time, an increase in total protein contents (more than 50% after 14D) as well as a decrease in acetylcholine esterase activity were reported for all treatments. We were also able to identify the induction of oxidative stress after xenobiotic exposure, which is more pronounced at the end of the treatment (14D), resulting in the stimulation of the antioxidant system (gluthione, glutathione S-transférase, catalase) as well as the induction of lipoxygenase, which is responsible for the oxidation of polyunsaturated fatty acids as well as the generation of reactive oxygen species (ROS) involved in the inflammatory phenomenon. Finally, it turns out that the species Aporrectodea caliginosa is sensitive to the different concentrations applied, even those used in the open field, and that Decis (deltamethrin) seems to be more toxic than Prosaro and that the combinaison P2/D2 is as toxic as Decis alone (D2).

Production and meat quality of broiler chickens with the use of a probiotic complex of bifidobacteria and lactobacilli

The prohibition of antibacterial agents in animal husbandry has spurred research into the creation and study of new probiotic supplements aimed at normalizing the gut microbiota to ensure colonization resistance of the host organism. This resistance prevents the colonization of the gastrointestinal tract by pathogenic and opportunistic microflora. Scientists from many countries have developed a range of preparations based on the normal gut microbiota, such as lactobacilli and bifidobacteria, which are used to maintain and restore the biocenosis of the digestive tract. These probiotics are also effective therapeutic and preventive agents for poultry digestive tract diseases, helping correct gut microbiocenosis. In our study, we investigated the impact of the combined probiotic preparation "TIMM-P" on the productive characteristics of poultry and the quality and preservation of broiler chicken meat. This study determined clinical indicators, absolute and average daily weight gains, the meat quality of broiler chickens, and its chemical composition under the influence of the probiotic supplement. It was found that administering "TIMM-P" in courses on days 1–5, 21–25, and 30–35 positively influences the growth of muscle tissue in poultry. Determination of the chemical composition of meat in 42-day-old experimental broiler chickens indicated a significant increase in total protein content in the muscle tissue using the probiotic. Additionally, the absolute and average daily weight gains of the broiler chickens in the experimental group significantly increased during days 15–28 and 29–42 of the study. We observed a significant impact of the probiotic preparation on extending the shelf life of poultry meat based on the study of organoleptic and chemical indicators of broiler chicken meat during refrigerated storage. On the 7th and 9th days of storage, the carcasses of the experimental group that received the probiotic showed a significant decrease in pH levels, acid number, and peroxide number of the fat in the meat. Thus, oral administration of the probiotic preparation increases live weight gains, improves meat quality indicators of broiler chickens, and extends its shelf life. The obtained results have important practical significance as they scientifically substantiate the use of probiotic preparation as a means to preserve livestock, increase live weight gains of broiler chickens, and improve poultry meat quality. Therefore, the complex probiotic preparation "TIMM-P" can be recommended for use in broiler chicken farming.

Effects of dietary supplementation of polysaccharide from Agaricus blazei Murr on productive performance, egg quality, blood metabolites, intestinal morphology and microbiota of Korean quail.

This study aimed to investigate the effects of dietary supplementation with Agaricus blazei polysaccharide (ABP) at varying concentrations on the performance, egg quality, blood biochemistry, intestinal morphology, and microflora of quail. The study involved a total of 2,700 Korean quails, which were randomly divided into three groups. The measured variables encompassed productive performance, egg parameters, carcass parameters, serum metabolites, immune response parameters, antioxidative properties, and gut microbiome. The addition of ABP did not have a significant effect on average daily feed intake. However, it was found to increase the average daily egg weight and egg production rate, reduce the feed-egg ratio. There were no significant impacts on egg quality measures such as egg shape index, egg yolk index and color, egg yolk and protein content. However, ABP supplementation significantly increased the Hough unit (p<0.01) and decreased the rate of unqualified eggs (p<0.01). Regarding serum parameters, the inclusion led to an increase in total protein concentration (p<0.05) and a reduction in low-density lipoprotein cholesterol (p<0.05). There were no significant effects observed on immune indicators such as immunoglobulin A (IgA) and IgM. ABP supplementation increased the levels of serum antioxidant indicators, including glutathione peroxidase, total superoxide dismutase (p<0.05), and total antioxidant capacity colorimeter (p<0.05). Furthermore, ABP supplementation significantly elevated the intramuscular fatty acid content in quail meat. Additionally, ABP supplementation demonstrated a significant improvement in the diversity of gut microbiota and induced alterations in the composition of the gut microbiota. The findings of this study indicate that dietary supplementation of ABP enhanced production performance and antioxidant capacity while increasing the levels of polyunsaturated fatty acids in quail muscle.

Exploration and Profiling of Potential Thermo-alkaliphilic Bacillus licheniformis and Burkholderia sp. from varied Soil of Delhi region, India and their Plant Growth-Promoting Traits

Soilless cultivation has emerged as a fundamental alternative for large-scale vegetable production because it generates high-quality yields and uses resources efficiently. While plant growth-promoting bacteria (PGPB) are known to enhance growth and physiological aspects in crops grown in soil, their application in soilless cultivation has been relatively less explored. This study aimed to isolate potential PGPBs from soil samples collected from five locations in and around the Delhi-National Capital Region (NCR), India, which were further screened for significant PGPB attributes. Among these, 51 isolated were selected for assessing the impact on Oryza sativa (rice) growth and yield grown on a hydroponic set. The results indicated that isolates AFSI16 and ACSI02 significantly improved the physiological parameters of the plants. For instance, treatment with AFSI16 showed a 23.27% increase in maximum fresh shoot mass, while ACSI02 resulted in a 46.8% increase in root fresh mass. Additionally, ACSI02 exhibited the highest shoot length (34.07%), whereas AFSI16 exhibited the longest root length (46.08%) in O.sativa. Treatment with AFSI16 also led to significant increases in total protein content (4.94%) and chlorophyll content (23.44%), while ACSI02 treatment showed a 13.48% increase in maximum carotenoid content in the leaves. The potential PGPBs were identified through 16S rRNA sequencing, as the two most effective strains, AFSI16 and ACSI02, belonged to thermo-alkaliphilic Bacillus licheniformis and Burkholderia sp., respectively. This study demonstrated the potential of these identified PGPB strains in enhancing crop performance, specifically in soilless cultivation systems.

Anti-diabetic impact of ‘Aju Mbaise’ herbal cocktail in streptozotocin-induced diabetic female rats

This study evaluated the hypoglycaemic potential of ‘Aju Mbaise’ herbal mixture. Female Wistar rats of 40 – 50 g and fifty-four (54) in number were obtained and housed at the animal house of the Department of Pharmacology, University of Port Harcourt. They were assembled into 6 groups of 9 rats each. Group I served as the normal control (NC) while the remaining five groups were induced with diabetes type 2 using high-fat diet (HFD) for about 8 weeks and streptozotocin at 35 mg/kg body weight. Group II was the diabetic control (DC) while the other groups (III, IV, V &amp; VI) were administered 7.2 mg/kg metformin and the cocktail extract at three different concentrations of 500 mg/kg, 250 mg/kg, and 100 mg/kg respectively. Biochemical tests were conducted after twelve weeks of treatment. The blood glucose concentration was estimated using the Accu-check glucometer and glucose strips while the rest biomarkers were analyzed using their specific test kits. Diabetes mellitus was confirmed after HFD and STZ administration. Result obtained showed that the normal and metformin control groups recorded significant (p&lt;0.05) lower blood glucose, liver enzymes (AST, ALT, &amp; ALP), urea, and creatinine, as well as higher total protein concentrations when compared to the diabetic control and treated groups. Also, significant (p&lt;0.05) reduction in blood glucose, liver enzymes, urea, and creatinine, as well as increase in total protein concentrations was recorded by the treated groups when compared to the diabetic control group. This study revealed the potential hypoglycaemic effect of ‘Aju Mbaise’ herbal cocktail.

Silicon-Selenium Interplay Imparts Cadmium Resistance in Wheat through an Up-Regulating Antioxidant System.

Cadmium (Cd), being a highly toxic heavy metal, significantly impacts plant growth and development by altering nutrient uptake and causing oxidative and structural damage, resulting in reduced yield. To combat Cd toxicity and accumulation in wheat, it was hypothesized that co-application of Selenium (Se) and Silicon (Si) can reduce the adverse effect of Cd and regulate Cd resistance while improving Se fortification in wheat. Therefore, this study evaluated the comparative effect of Se and Si on the growth and antioxidant defense systems of wheat plants grown in a hydroponic setup. Briefly, the plants were acclimatized to the hydroponic solution for 1 week and then exposed to 10 µmol Cd. Afterwards, the treatments, including 0.2 mmol Si and 1.5 µmol Se, were applied as a root and foliar application, respectively. Plants supplemented with both Se and Si showed improved biomass and other physiological growth attributes, and this response was associated with improved activity/contents of antioxidants, including glutathione (GSH) content, glutathione reductase (GR), ascorbate peroxidase (APX), and catalase (CAT), with related lowering of hydrogen peroxide, malondialdehyde content, and structural damages. Moreover, by Se + Si supplementation, a decrease in total S levels in plant tissues was observed, whereas an increase in total protein concentration and GSH indicated a different and novel mechanism of Cd tolerance and S homeostasis in the plant. It was observed that Si was more involved in significantly reducing Cd translocation by stabilizing Cd in the root and reducing its content in the soluble fraction in both the root and shoot. Whereas Se was found to play the main role in reducing the oxidative damage caused by Cd, and the effect was more profound in the shoot. In addition, this study also observed a positive correlation between Si and Se for relative uptake, which had not been reported earlier. Our findings show that the Se and Si doses together benefit growth regulation and nutrient uptake; additionally, their combinations support the Cd resistance mechanism in wheat through upregulation of the antioxidant system and control of Cd translocation and subcellular distribution, ultimately contributing to the nutritional quality of wheat produced. Thus, it is concluded that the co-application of Se and Si has improved the nutritional quality while reducing the Cd risk in wheat and therefore needs to be employed as a potential strategy to ensure food safety in a Cd-contaminated environment.

Вплив S-естерів тіосульфокислот на окремі біохімічні показники крові щурів

The effect of S-esters of thiosulfonic acids - S-ethyl-4-aminobenzenethiosulfonate (ETS), S-allyl-4-aminobenzenethiosulfonate (ATS) and S-allyl-4-acetylaminobenzenethiosulfonate (AATS) at doses of 50 and 100 mg/kg body weight in rats on some biochemical parameters of protein and lipid metabolism in rats was investigated. The study was conducted in two stages on white male laboratory rats of the Wistar line, weighing 190–210 g. During the first stage of the study, animals of all experimental groups were administered by 500 µL of oil solutions of thiosulfonate esters at 100 mg per kg of body weight for 21 days. In the second stage of the study, the dosage was reduced to 50 mg per kg of body. Throughout both stages of the study, rats were divided into a control group (I) and three experimental groups (II, III, IV), with 5 animals in each. Rats in group II received ETS with their food, while group III received ATS, and group IV received AATS, all in appropriate doses. The animals of the control group were similarly given 500 µL of oil once a day in their diet. After the decapitation of animals, the activities of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, concentrations of total protein, urea, cholesterol and triacylglycerols were determined in blood plasma. As a result of the study, it was found that the use of sulfonic acid esters in the studied doses did not have a hepatotoxic effect on rats, as indicated by the normal activity of liver-indicating enzymes (ALT, ALP and alkaline phosphatase) in the blood plasma. In addition, sulfonic acid esters promoted protein synthesizing function, as evidenced by an increase in total protein concentration in the blood plasma and a decrease in urea – the end product of their decomposition. The studied esters had a positive effect on lipid metabolism, which was confirmed by a decrease in the concentration of triacylglycerol and cholesterol in the blood plasma, which, in turn, may contribute to the inhibition of the formation of their complexes in the liver - low-density lipoproteins. Probable changes in the concentration of total protein under the action of ETS and ATS and triacylglycerols under the action of ATS and AATS showed better effects of sulfonic acid esters at a dose of 100 mg/kg compared to a dose 50 mg/kg.

Некоторые биохимические изменения хрусталика в процессе старения.

The goal is to study some biochemical changes in the lens of rats and humans during aging. Materials and methods. Experiments were carried out on 25 rats. We also examined 84 patients with initial nuclear cataract. Results. diverse dynamics of the content of total lipids in the lenses of rats was revealed. There is a noticeable decrease in the content of products of oxidative modification of lipids in the lens tissue between 12 and 24 months and a slight increase in the period from 24 to 36 months. A 2-fold decrease in the content of the total protein level was revealed when comparing groups of 5 months and 36 months and an increase in total protein content with age. When comparing the parameters of oxidative degradation, cl in the lens tissue of rats and humans with age, similar indicators of dynamics were obtained. Conclusion. The data obtained indicate changes in the parameters of lipids and proteins in the lens tissue of rats during postnatal ontogenesis. The fact that there is a balance between damage and protection of proteins in the transparent lens was revealed. When comparing data on protein oxidation in the lens tissue of rats and humans with age, similar indicators were obtained. Keywords: lens, aging, biochemical changes

Estimation of Prolactin and Some Biochemical Parameters in Some Psychotic Male Patients Treated with Risperidone in Tikrit City

The primary purpose of this research is to examine the impact of taking risperidone by psychotic individuals on serum levels of prolactin hormone as well as a few biochemical indicators (total protein and liver enzymes AST and ALT). The experiment required collecting fifty blood samples and dividing them into two groups: the first group consisted of normal, healthy persons who served as a control. In contrast, the second group consisted of psychotic patients treated with risperidone at a dosage of two milligrams per day. The findings of this study showed that There were significant increases in total protein content at levels (P≤ 0.05) of the group 2 compared to control, and there were significant gains in liver enzymes (AST and ALT) concentration range at levels (P ≤0.05) of the second group in comparison to the control group, and there were substantial improvements increases in prolactin hormone concentrations at levels (P≤ 0.05) of the second group in compared to control.

C57Bl/6N mice have an attenuated lung inflammatory response to dsRNA compared to C57Bl/6J and BALB/c mice

BackgroundLower respiratory infections caused by ssRNA viruses are a major health burden globally. Translational mouse models are a valuable tool for medical research, including research on respiratory viral infections. In in vivo mouse models, synthetic dsRNA can be used as a surrogate for ssRNA virus replication. However, studies investigating how genetic background of mice impacts the murine lung inflammatory response to dsRNA is lacking. Hence, we have compared lung immunological responses of BALB/c, C57Bl/6N and C57Bl/6J mice to synthetic dsRNA.MethodsdsRNA was administered intranasally to BALB/c, C57Bl/6N and C57Bl/6J mice once/day for three consecutive days. Lactate dehydrogenase (LDH) activity, inflammatory cells, and total protein concentration were analyzed in bronchoalveolar lavage fluid (BALF). Pattern recognition receptors levels (TLR3, MDA5 and RIG-I) were measured in lung homogenates using RT-qPCR and western blot. Gene expression of IFN-β, TNF-α, IL-1β and CXCL1 was assessed in lung homogenates by RT-qPCR. ELISA was used to analyze protein concentrations of CXCL1 and IL-1β in BALF and lung homogenates.ResultsBALB/c and C57Bl/6J mice showed infiltration of neutrophils to the lung, and an increase in total protein concentration and LDH activity in response to dsRNA administration. Only modest increases in these parameters were observed for C57Bl/6N mice. Similarly, dsRNA administration evoked an upregulation of MDA5 and RIG-I gene and protein expression in BALB/c and C57Bl/6J, but not C57Bl/6N, mice. Further, dsRNA provoked an increase in gene expression of TNF-α in BALB/c and C57Bl/6J mice, IL-1β only in C57Bl/6N mice and CXCL1 exclusively in BALB/c mice. BALF levels of CXCL1 and IL-1β were increased in BALB/c and C57Bl/6J mice in response to dsRNA, whereas the response of C57Bl/6N was blunt. Overall, inter-strain comparisons of the lung reactivity to dsRNA revealed that BALB/c, followed by C57Bl/6J, had the most pronounced respiratory inflammatory responses, while the responses of C57Bl/6N mice were attenuated.ConclusionsWe report clear differences of the lung innate inflammatory response to dsRNA between BALB/c, C57Bl/6J and C57Bl/6N mice. Of particular note, the highlighted differences in the inflammatory response of C57Bl/6J and C57Bl/6N substrains underscore the value of strain selection in mouse models of respiratory viral infections.

Kidney protective and burn healing effect Plantago lanceolata aqueous extract on albino male mice

This study aimed to in vivo evaluate the effect of Plantago lanceolata aqueous extract on kidney function test ( total serum protein, urea and creatinine) and anti-inflammatory effect through determined the day require to heal burn in mice skin.. Plantago lanceolata is a species of flowering plant in the Plantaginaceae (plantain) family, Plantago lanceolata seems to be a very versatile in addition to the medicine cabinet being an antifungal, antioxidant, analgesic and many other medicinal application. Two design of mice groups used: one for kidney protective and other for anti-inflammatory activity. In kidney protective activity eight mice groups were assessed: group I: mice treated with first plant dose (500 mg\kg) , group II: mice treated with second plant dose (250 mg\kg), Group III :negative control; mice administrated with distill water, Group IV: mice were administrated with CCL4, Group V: An interaction between CCL4 and first dose of plant extract (0.02 mg\kg CCL4+ 500 mg\kg plant dose), Group VI: An interaction between CCL4 and second dose of plant extract (0.02 mg\kg CCL4+ 250 mg\kg plant dose), Group VII:: An interaction between first dose of plant extract and CCL4 (500 mg\kg plant dose +0.02 mg\kg CCL4), Group VIII: An interaction between second dose of plant extract and CCL4 (250 mg\kg plant dose +0.02 mg\kg CCL4). While for anti-inflammatory activity, three mice groups were assessed: Group I: Mice without any treatment, Group II: Mice were administrated with Plantago lanceolata and Group III: Mice were administrated with silverin cream of burns. The results showed that CCL4 drug produced an experiential decrease in protein concentration (3.76±0.15 g\dL) in contrast with negative control (5.40±0.26 g\dL), while direction of plant and plant with CCL4 at all doses tested instigated an increase in total protein concentration (7.46±0.40, 6.43±0.41, 6.43±0.41 and 5.60±0.73 g\dl) for groups (V, VI, VII and VIII respectively).Creatinin also match other results of kidney function by increasing their concentrations in mice treated with CCL4 (0.80±0.10 mg\dL ) as compared to negative control (0.3±0.1 mg\dl) while plant extract showed the ability to cause reduction in a concentration in a dose dependent manner and according to deferent treatments (0.60±0.10, 0.62±0.15, 0.36±0.05 and 0.50±0.10 mg\dl) for (group V,VI,VII and VIII respectively). The results of anti-inflammatory activity declared the ability of plant to heal burn in 14 days as compared to negative and positive controls which require 28 and 18 days) respectively. All obtained results may be attributed to active secondary metabolite presented in plant like tannin, mucilage and silicic acid etc.

PSI-11 Effect of Garlic Barrier on Fecal egg Count and Protein Secretion in Goats

Abstract The present study was carried out to determine the effect of garlic on egg counts and secretion of Galectins (Gals) in goat blood. Garlic (Allium sativum) has been used as medicinal herb since time immemorial in almost every known civilization. It has antibiotic, anti-helminthic and anti-inflammatory properties. Galectins (Gals) are a family of β-galactosidase binding proteins that have a conserved carbohydrate recognition domain. They are involved in cell adhesion, migration, activation, proliferation, apoptosis and modulate pathological processes such as inflammation. Twelve (N=12) adult female clinically healthy Boer x Spanish goats were divided into two treatment groups (N=6 each) used. Treatment group 1 was drenched with 10 ml of Garlic barrier as recommended by the manufacturer (Garlic Barrier, Glendale, CA) and Treatment group 2 was drenched with water over a four-week period. Fecal and blood samples were collected weekly. Eggs per gram was evaluated using Modified McMaster method and the total plasma protein concentration was assessed using the BCA assay (Pierce). All variables were assessed using a t-test (SAS analysis software version 9.0 at p≤0.05). Garlic treatment affected the fecal egg count and plasma protein concentration when compared to untreated controls. The fecal egg count was reduced in animals treated with garlic. The effect varied over time. Garlic EPG in weeks 1 (p&amp;lt;0.004) and 3 (p&amp;lt;0.027) of treatment. A significant increase in total protein concentration (p&amp;lt;0.004) was observed. Garlic treatment has an effect on GIN levels and the inflammatory response. The time dependent effects may indicate parasite, infection stage specific targets and need further study.

Antioxidant enzymes activity and membrane thermostability in durum wheat genotypes under heat stress

Relevance. Insufficient tolerance to extremely high temperatures is one of the main reasons for the decline in crop yields.Methods. Two contrasting durum wheat genotypes (Triticum durum Desf.) were used in the present study: Barakatli 95 (tolerant genotype) and Garagylchyg 2 (stress-sensitive genotype). The activity of antioxidant enzymes, ascorbate peroxidase (APO), catalase (CAT), guaiacolperoxidase (GPO), and benzidine peroxidase (BPO), were studied spectrophotometrically. The membrane thermostability was determined by the release of electrolytes from intact plant tissue.Results. It was revealed that guaiacolperoxidase plays a key role in the elimination of reactive oxygen species when exposed to high temperatures, since a significant increase in the activity of this enzyme was observed during short-term thermal stress. Heat stress caused a slight decrease in catalase activity and a significant decrease in the activity of ascorbate peroxidase and benzidine peroxidase. An increase in total protein concentration was observed under heat stress. The indicators of the electrolyte leakage from leaf tissues confirm the variety-specific thermostability of wheat membranes. The membrane damage rate (MDR) increased in direct proportion to the heating temperature and showed the highest value in the sensitive Garagylchyg 2 variety. According to the electrolyte leackage parameters from leaf tissues during hyperthermia, was concluded variety-specific thermal stability of wheat plant membranes.

Prolonged heat and drought versus cool climate on the Swedish spring wheat breeding lines: Impact on the gluten protein quality and grain microstructure

AbstractFluctuating climate, heat, and drought are expected to considerably impact bread wheat (Triticum aestivum) quality in the coming years and, as wheat is an essential food element worldwide, this will have significant implications for future food security and the global economy. This leads to an urgent need for developing wheat varieties with stable yield and gluten quality. In this study, we investigated the effect of heat and drought, compared to a cool climate, on gluten proteins in 294 spring wheat genotypes grown in 2017 and 2018 in Sweden. Gluten protein parameters were studied by size exclusion high‐performance liquid chromatography (SE‐HPLC) and grain morphology by X‐ray tomography. The prolonged heat and drought led to: (i) increased gluten polymerization and the formation of large polymers, as defined by the percentage of unextractable polymers in total polymers (%UPP) and the percentage of large unextractable polymers in total large polymers (%LUPP); and (ii) increase in large monomers, as defined by the percentage of large unextractable monomers in the total large monomers (%LUMP) and the ratio of monomers versus polymers (Mon/Pol) in the flour. The cooler climate also led to an increase in total protein concentration and accumulation of the monomeric proteins and total SDS‐extractable proteins (TOTE). No difference in the total amount of SDS‐unextractable proteins (TOTU) was found between the studied climates. Due to the heat and drought stress, the grain yield decreased in most of the genotypes, while the grain microstructure varied only to a minor extent. The wheat genotypes identified in the study that provide good yields and stable gluten properties in both prolonged heat–drought and cool environments are strong candidates to contribute to a secure, self‐sufficient future wheat supply in the face of an evolving climate in Sweden and in similar climates worldwide.

IMPACT OF CHLORPYRIFOS ON BACILLUS CEREUS OP3 SOIL BACTERIA ISOLATED FROM RHIZOSPHERE

Chlorpyrifos is a pesticide belongs to organophosphate group and widely used in Indian agriculture. The evaluation of chlorpyrifos removal from the soil using pesticide degrading bacteria will help in the resilience of soil fertility. Bacteria capable of degrading chlorpyrifos were isolated from chlorpyrifos-treated agricultural soil. The degradation of chlorpyrifos by a potential isolate was determined using FTIR and quantified using the molecular extinction coefficient in an ELISA reader. GC-MS was used to analyze the chlorpyrifos degradation metabolites. Additionally, the effect of chlorpyrifos exposure on genomic DNA and total protein content was determined. A total of 174 bacterial colonies were isolated, of which only 40 bacterial colonies were pesticide tolerant in 100ppm chlorpyrifos. Among them, only three isolates (OP3, OP5, and OP7) demonstrated tolerance above 100ppm. Within five days of incubation, OP3 demonstrated tolerance to chlorpyrifos concentrations up to 300ppm and 47 percent of chlorpyrifos was removed. By 16S rRNA sequencing, potential OP3 isolates were identified as Bacillus cereus OP3. FTIR spectra confirmed that B. cereus degraded chlorpyrifos, with 92.4 percent of chlorpyrifos degraded. In GC-MS analysis, metabolites such as MNitroaniline and 4-Methyl-2-{(4-Nitrophenyl)Thio}Pyrimidine were detected. Chlorpyrifos overexposure alters the total protein content of exposed cells and induces DNA breaks. As a result, it is concluded that B. cereus is able to degrade chlorpyrifos and the excessive use of pesticides make some changes in the soil bacteria. Overexposure of chlorpyrifos leads to an increase in total protein content and induce DNA strands breaks in pesticide degrading Bacillus cereus.

Prospects for the use of a &lt;i&gt;Bacillus subtilis&lt;/i&gt; metabolites-based feed additive in dairy farming

Laboratory and field experiment on use of the feed additive based on Bacillus subtilis endo- and exometabolites for the cows in different physiological periods are described in the paper. The feed additive impact on main body systems of the tested cows (n = 30), milk production parameters, growth rate of the calves (n = 18) born to the said cows were examined. The feed additive was added to the diets for the cows of test groups, 15 g per cow. The feed additive was found to have a positive effect on immunohematological and metabolic processes in postpartum cows. Neutrophils’ phagocytic activity increased by 12.5% and 14.6% in the animals of test group 1 and test group 2, respectively, as compared to that one in control animals (42.8 ± 1.9%). Neutrophil absorbency increased by 2.5 times, 3.2 times and 2.1 times in the animals of test group 1, test group 2 and control group, respectively. The proportion of T-lymphocytes in blood of animals in test group 1 and test group 2 was 44.5 and 48.9%, respectively, proportion of T-lymphocytes in blood of control animals equaled to 37.5%. Trend for increase in total protein concentration in cow sera owing to increase in albumin fraction was observed in postpartum period: it was 72.91 ± 3.45 g/l in test group 1; 75.54 ± 4.12 g/l in test group 2; 70.95 ± 4.25 g/l in control group. Average daily milk yield in cows of test group 1, test group 2 and control group for the 150 days of lactation was 24.50 ± 1.86 kg; 25.33 ± 1.45 kg and 22.75 ± 4.41 kg, respectively. Higher growth rate was reported for the calves born to the cows received the diet supplemented with the said feed additive. Heifers of test group 1 and test group 2 have reached body weight of 193.51 ± 5.76 and 195.33 ± 3.76 kg and in control group – of 187.33 ± 4.98 kg within 6 months. Feed additive based on endo- and exometabolites of Bacillus subtilis is recommended for cow diets for highly productive dairy herd creation and food-producing animal welfare maintenance.