Increase In Tension Research Articles

Адаптационные механизмы российского рынка труда в условиях неопределенности

The relevance of the study is due to the continuous changes in the labor market under the influence of external factors and the need to increase its adaptive abilities in conditions of uncertainty. The purpose of the study is to identify the features of the adaptation of the Russian labor market to the conditions of uncertainty caused by the pandemic, and the development of new forms of employment that ensure the structural transformation of the market and changes in labor relations. The objectives of the study are to analyze new forms of work arising on the basis of transformation of models of interaction between participants in a period of uncertainty; to determine the vector of development of labor relations that ensure the balance of the labor market. Methodology . The study was carried out using methods of theoretical analysis, systematization, economic analysis, graphical method, comparative analysis method, and morphological analysis method. Results . The analysis of the dynamics of the labor market development indicators during the pandemic allowed us to conclude that it was destabilized in the conditions of uncertainty, manifested in the form of a decrease in employment indicators, an increase in unemployment, an increase in tension in the market. By 2021, many indicators of the labor market development have returned to a doc-like level. The activity of the Russian population in the global online labor market is analyzed, as well as the demand of Russian companies for labor on digital platforms. The directions of ensuring greater adaptability of the Russian labor market through the use of various mechanisms of state policy are substantiated. Conclusions . The emergence of new forms of work based on the transformation of models of interaction between participants has been established. It is concluded that accelerated digitalization during the pandemic created opportunities for online employment of the population, which contributed to the partial removal of tension in the Russian labor market.

Analysis of the Impact of Tourmaline and Terahertz-Infused Clothing on Heart Rate Variability

Introduction: Expanding upon prior investigations that identified advantageous Far Infrared (FIR) properties associated with two minerals, namely tourmaline and terahertz, across diverse domains, including health and medicine. This study explores the impact of incorporating fine powders of these minerals into printing ink for patterns on shirts on HRV when wearing the shirts. Methods: A total of 12 healthy adult males were surveyed in this study. Fine powders of the two minerals, tourmaline, and terahertz were incorporated into printing ink and used to print patterns on shirts. The impact of wearing these shirts on HRV was observed. Result: The significant differences in time-domain analysis parameters such as SDRR and RMSSD, frequency-domain analysis parameters including Total (ms2), VLF (ms2), LF (nu), HF (ms2), and nonlinear analysis parameters SD1 and SD2 were observed. Conclusion: The findings suggest that both short-term and long-term wear of shirts containing these minerals lead to an increase in vagal nerve tension. Participants wearing shirts with mineral inclusions exhibited changes related to vagal nerve activity.

“Ukraine could be the center of World War III!"

Üsküdar University Center for Strategic Studies Deputy Director Dr. Güler Kalay evaluated the reasons for the increase in global geopolitical tensions and possible scenarios of World War III.

Lipid composition and physicochemical parameters of unrefined sunflower oil

Unrefined sunflower oil is obtained by pressing of the sunflower seeds and subsequent fine filtration. It contains more biologically active substances compared to the refined one, it is more stable to oxidation processes, for that reason it is widely used in the composition of the various food products. The main acids in the oil are oleic (56.5%), linoleic (31.7%), palmitic (6.4%), and stearic acid (3.2%). Total sterol content is 0.7%, with ß-sitosterol as a main component (84.0%). The quantity of tocopherol is 188 mg/kg and it consists of one compound α-tocopherol (100%). Different physicochemical parameters were obtained at temperature ranges (from 20 to 80°C). The values of these parameters decrease with an increase in temperature, density (from 0.938 to 0.901 g/ml), surface tension (from 44.124 to 31.787 mN/m), dynamic viscosity (from 29.195 to 14.913 mPa.s), and kinematic viscosity (from 31.125 to 16.552 mm2/s). All these changes are in confirmation of the data from the literature and are relevant in our next studies.

Колабораціонізм на окупованих територіях в освітянській сфері у період 2014–2024 рр.

The article considered the problem of collaborationism among Ukrainian educators who are in the occupied territory of the country. The spread of collaborationism is associated with the active use of various methods of hybrid warfare by the Russian Federation, which led to Ukraine’s temporary loss of territorial integrity and the occupation of part of its territories. The social danger of collaborationism is manifested in the threat to national security, the commission of crimes against humanity, the increase of social tension and the violation of international law. Collaborative activity in the field of education means the conscious participation of a citizen of Ukraine in the performance of these tasks in the occupied territory for the benefit of the aggressor state. It has been found that the main reasons for committing collaborative acts in the educational sphere are the preservation of the workplace, the desire for career growth, financial reward or other selfish motives, and the desire for revenge. In the educational process, key manifestations of collaborationism are the implementation of educational standards of the aggressor state and the implementation of propaganda in educational institutions. Some examples of collaborationism in the occupied territory of Ukraine from 2014 to the present day, changes in curricula and teaching methods are analyzed. By using various techniques, such as bribing heads of educational institutions, teachers, parents, transferring universities and creating alternative educational institutions, the occupiers try to gain control over the educational process in the occupied territories. Teachers who work in the occupied territories under Russian programs become accomplices and collaborators of the occupying power. Possible ways to overcome the phenomenon of collaborationism in the future are highlighted. To date, educators who are in the temporarily occupied territories and cannot leave for the territories controlled by Ukraine are recommended to document by any available means the facts of coercion to cooperate with the occupiers. In the future, the authorities should ensure the transparency and publicity of the system of punishments for collaborationism. Collaborators must be prosecuted and held accountable for their actions before the law.

Numerical study on the characteristics of viscous fingering during the displacement process of non-Newtonian fluid.

This study uses numerical methods (ANSYS-Fluent) to investigate the viscous fingering of the displaced phase as a shear-thinning fluid in the classic three-dimensional Hele-Shaw cell. Comparing the behavior of fingerings with different properties on the upper and lower surfaces of a three-dimensional model, it was found that when the upper and lower surfaces are walls, under the combined action of moving contact lines and Saffman-Taylor instability, fingering splitting occurs at the tip, resulting in the appearance of two fingers at the interface. In addition, we have found that interfacial tension has a suppressive effect on short waves. As the interfacial tension increases, the velocity at the advancing tip decreases. Therefore, when the interface tension is 0, viscous fingering displacement reaches the farthest distance. We have also conducted research on the viscous fingering at different temperatures. The results indicate that increasing the temperature leads to a decrease in the viscosity of the displaced phase, making the flow more stable. As the temperature rises, the pressure gradient inside the flow path increases, pushing the viscous fingering further.

Western churches and politics: frontiers of ideologic divergence

The article deals with conflict lines in the interaction of religious institutions and political actors in Europe. Church policy in the West is seen as a practical implementation of the neo-secular approach, which implies a reduction in the role of religious institutions and church elites while remaining religiosity and quasi-religiousness. It is shown that the contradictions of state-confessional relations in Western countries pose both a threat to the value autonomy of religious organizations and an increasing trend towards the instrumentalization of religion in foreign policy. It is shown that attempts of value pressure on traditional church institutions contribute to the formation of a hostile environment that contributes to the strengthening of intra-church contradictions. As a result, religious organizations find themselves in a situation of multiplying ideological divisions and artificially pushed internal competition. The risks of reducing the institutional stability of traditional churches based on the unity of the doctrine and striving for internal unity are increasing. In this regard, the article offers an analysis of possible internal and external reactions of church institutions to the increasing pressure. As possible scenarios, the strengthening of the processes of ideological and regional autonomy and the increase in increasingly open conflicts with secular authorities in a number of countries on topical issues of the political agenda are considered. Under any of the scenarios, it is predicted that the fault line between European countries will strengthen on issues with a strong religious connotation, which may lead to an increase in social tension and deepening of divisions among the electorate in the Western states.

Surface and curvature tensions of cold quark matter in the SU(3)fNJL model with vector interactions

AbstractWe investigate the surface and curvature tensions of three‐flavor cold quark matter in a state of electric charge neutrality and chemical equilibrium under weak interactions, employing the Nambu–Jona–Lasinio model with the inclusion of vector interactions. To account for finite‐size effects we adopt the multiple‐reflection expansion framework and present our results for various input parameters. Our results indicate that both surface and curvature tensions increase with heightened vector interactions and density. Furthermore, we note that the restoration of chiral symmetry in the strange quark sector leads to a modest reduction in curvature tension and a substantial increase in surface tension.

Effect of surfactant surface and interfacial tension reduction on infiltration into hydrophobic porous media

Surfactant molecules increase the infiltration rate into hydrophobic porous media by lowering the infiltrating water's surface tension and the interfacial tension between hydrophobic surfaces and water molecules. We investigated the relative effect of these rate-limiting processes on the infiltration rate of aqueous surfactant solution into hydrophobic porous media. Two surfactants at various concentrations were applied at a constant pressure head to 1D columns filled with hydrophobic soil, and water was applied at the same pressure head to columns filled with surfactant-pretreated hydrophobic soil. Based on the measured contact angle, surfactant pretreatment significantly reduced the hydrophobic soil's interfacial surface tension, which increased the infiltration rate compared to the direct aqueous surfactant application to the hydrophobic soil. The latter's slower infiltration rate was attributed to the depletion of surfactant molecules due to its adsorption to the hydrophobic molecules near the advancing wetting front, yielding an increase in the surface tension of the infiltrating solution. Surfactant pretreatment increased the opportunity time for surfactant adsorption to the hydrophobic molecules, resulting in interfacial tension reduction and infiltration rate increase. Diffusion-limited surfactant adsorption on the hydrophobic surfaces, leading to reduced interfacial tension between the surface and infiltrating liquid, had a greater impact on limiting infiltration into hydrophobic porous media compared to the reduction in surface tension of the infiltrating liquid due to surfactant presence.

Effective cell membrane tension protects red blood cells against malaria invasion.

A critical step in how malaria parasites invade red blood cells (RBCs) is the wrapping of the membrane around the egg-shaped merozoites. Recent experiments have revealed that RBCs can be protected from malaria invasion by high membrane tension. While cellular and biochemical aspects of parasite actomyosin motor forces during the malaria invasion have been well studied, the important role of the biophysical forces induced by the RBC membrane-cytoskeleton composite has not yet been fully understood. In this study, we use a theoretical model for lipid bilayer mechanics, cytoskeleton deformation, and membrane-merozoite interactions to systematically investigate the influence of effective RBC membrane tension, which includes contributions from the lipid bilayer tension, spontaneous tension, interfacial tension, and the resistance of cytoskeleton against shear deformation on the progression of membrane wrapping during the process of malaria invasion. Our model reveals that this effective membrane tension creates a wrapping energy barrier for a complete merozoite entry. We calculate the tension threshold required to impede the malaria invasion. We find that the tension threshold is a nonmonotonic function of spontaneous tension and undergoes a sharp transition from large to small values as the magnitude of interfacial tension increases. We also predict that the physical properties of the RBC cytoskeleton layer-particularly the resting length of the cytoskeleton-play key roles in specifying the degree of the membrane wrapping. We also found that the shear energy of cytoskeleton deformation diverges at the full wrapping state, suggesting the local disassembly of the cytoskeleton is required to complete the merozoite entry. Additionally, using our theoretical framework, we predict the landscape of myosin-mediated forces and the physical properties of the RBC membrane in regulating successful malaria invasion. Our findings on the crucial role of RBC membrane tension in inhibiting malaria invasion can have implications for developing novel antimalarial therapeutic or vaccine-based strategies.

Neurogenic Regulation of Cerebral Blood Flow

The brain has a well-developed vascular network, which allows it to consume up to 15% of the cardiac output with a low mass relative to the whole-body weight. Normally, the metabolic demands of the brain depend considerably on the intensity of functioning of its different departments, which requires constant regulation of the local blood flow level. On the other hand, the state of systemic hemodynamics can have a significant impact on the organ blood flow. Complex and multilevel mechanisms of the regulation of organ cerebral blood flow are aimed at minimizing the possible adverse effects of systemic hemodynamics impairment. The importance of precise and prompt regulation of cerebral blood flow is reinforced by the absence of energy reserves or substrates for its autonomous production in the nervous tissue. The main mechanisms of cerebral blood flow regulation include: myogenic regulation, influence of local humoral influences and vasoactive substances (hormones, metabolites) of systemic blood flow, changes in blood gas composition (increase or decrease in blood oxygen or carbon dioxide tension). In addition, endothelium-dependent mechanisms of regulation are distinguished. Finally, one more level of cerebral arterial tone regulation is represented by the effect of neurotransmitters released from vasomotor fibers terminals of sympathetic and parasympathetic sections of autonomic nervous system, as well as from subcortical neurons and cortical interneurons terminals. In the present review, the principles of neurogenic regulation of cerebral blood flow are considered. The neurogenic regulation of vascular tone is the most complex regulatory circuit. The autonomic innervation of cerebral vessels has significant features that distinguish it from that in most other organs of the great circulatory circle. In addition to the autonomic innervation proper, the vessels of the brain receive sensory innervation, and the small intracerebral arterioles are also innervated -directly by subcortical neurons and cortical interneurons. In this connection, a deeper understanding of the molecular mechanisms of the neurogenic regulation of cerebral blood flow may serve as a basis for the development of new methods of treatment of severe brain diseases based on neuromodulation in the long term.

A numerical study on the effect of wall wettability on film formation in liquid–liquid slug flow

This study numerically investigates the effect of the contact angle on the hydrodynamics of liquid–liquid slug flow in a capillary with an internal diameter of 2.4 mm. The finite volume method is used to solve the Navier–Stokes equation, and the volume-of-fluid method is employed for interface capturing. The contact angle varies from 13° to 169°. The aqueous phase is observed as a continuous medium, the organic phase is in a dispersed phase at low contact angles, and the aqueous phase is dispersed as a medium at high contact angles. The slug velocity is lower at partial wetting contact angles than at fully wetting or non-wetting contact angles. The vorticity inside the slug is higher at extreme contact angles (169°/13°) and becomes very pronounced near the interface. At the Weber number Weslug>0.135, fully developed film is observed. The thickness of this film increases as the interfacial tension decreases. Additionally, the pressure within the slug rises with an increase in interfacial tension.

Analysis of lateral dynamic response characteristics of drilling riser during installation of deep-water Christmas tree

In this research, in order to investigate the safety risk of drilling riser during deep-water Christmas tree lowering process and determine the basic parameters for the safety calculation of the upper part of the Christmas tree, wave-current force calculation method is explained based on modified Morison equation. According to differential element method, the dynamic equations of vertical and horizontal bending of riser under wave and current conditions are derived and equation solution method and calculation stability conditions are analyzed. By combining the upper and lower boundaries of riser during Christmas tree connection and environmental load factors, dynamic responses of riser under different wave, top tension, platform drift, operating water depth and other parameters are investigated. The obtained results show that maximum lateral displacement, corresponding water depth and bending moment of riser as well as the amplitude and period of displacement fluctuation at riser bottom are increased with the increase of wave velocity and water depth. It is also found that increase of top tension reduced the maximum lateral displacement of riser and increased corresponding water depth, but excessive tension increased the fluctuations of displacement and bending moment at riser bottom. Increase of platform drift increased maximum lateral displacement fluctuation range, bottom string displacement and bending moment of the riser and decreased corresponding maximum displacement water depth. Drift is found to increase the fluctuations of dynamic parameters at riser bottom. Increase of operating water depth increased riser maximum lateral displacement and decreased the period and amplitude of bottom bending moment fluctuations; also, maximum water depth position approached to "1/3" position on sea surface.

How Gender Shapes Sibling Tension in Adulthood Following Parental Death.

This study investigates gender differences in the effect of parents' deaths on sibling tension among bereaved adult children. Previous scholarship on adult sibling relations following the deaths of parents presents inconsistent results. These disparate findings may stem from past studies not taking into consideration the gender of both the deceased parent and the bereaved child. Analyses are based on three harmonized waves of quantitative and qualitative data collected from 654 adult children nested within 303 families as part of the Within-Family Differences Study. Multilevel models revealed that for daughters, but not sons, mothers' deaths in the past five years were associated with increases in sibling tension, whereas fathers' deaths did not predict changes in either sons' or daughters' sibling tension, regardless of timing. Qualitative analyses showed marked differences by child's gender in perceptions of patterns of shared work and support surrounding parents' deaths. Typically, sons expressed solidarity with siblings when mothers died and felt that the division of caregiving prior to mothers' deaths and arrangements following their deaths were fair. In contrast, daughters expressed increased solidarity with sisters surrounding mothers' deaths and disdain toward brothers who failed to contribute caregiving, support, or instrumental tasks. These findings underscore how gender of both parents and adult children differentially shape changes in adult children's relationships with their siblings in the face parental deaths, much as they do in other contexts across the life course.

Tension Remodeling Regulates Topological Transitions in Epithelial Tissues.

Cell neighbor exchanges play a critical role in regulating tissue fluidity during epithelial morphogenesis and repair. In vivo, these neighbor exchanges are often hindered by the formation of transiently stable fourfold vertices, which can develop into complex multicellular rosettes where five or more cell junctions meet. Despite their importance, the mechanical origins of multicellular rosettes have remained elusive, and current cellular models lack the ability to explain their formation and maintenance. Here we present a dynamic vertex model of epithelial tissues with strain-dependent tension remodeling and mechanical memory dissipation. We show that an increase in cell junction tension upon contraction and reduction in tension upon extension can stabilize higher-order vertices, temporarily stalling cell rearrangements. On the other hand, inducing mechanical memory dissipation via relaxation of junction strain and stress promotes the resolution of higher-order vertices, facilitating cell neighbor exchanges. We demonstrate that by tuning the rates of tension remodeling and mechanical memory dissipation, we can control topological transitions and tissue material properties, recapitulating complex cellular topologies seen in developing organisms.

Modeling ultrasound modulation of neural function in a single cell

BackgroundLow intensity ultrasound stimulation has been shown to non-invasively modulate neural function in the central nervous system (CNS) and peripheral nervous system (PNS) with high precision. Ultrasound sonication is capable of either excitation or inhibition, depending on the ultrasound parameters used. On the other hand, the mode of interaction of ultrasonic waves with the neural tissue for effective neuromodulation remains ambiguous. New methodHere within we propose a numerical model that incorporates the mechanical effects of ultrasound stimulation on the Hodgkin-Huxley (HH) neuron by incorporating the relation between increased external pressure and the membrane induced tension, with a stress on the flexoelectric effect on the neural membrane. The external pressure causes an increase in the total tension of the membrane thus affecting the probability of the ion channels being open after the conformational changes that those channels undergo. ResultsThe interplay between varying the acoustic intensities and frequencies depicts different action potential suppression rates, whereby a combination of low intensity and low frequency ultrasound sonication proved to be the most effective in modulating neural function.Comparison with Existing Methods: Our method solely depends on the HH model of a single neuron and the linear flexoelectric effect of the dielectric neural membrane, when under an ultrasound-induced mechanical strain, while varying the ion-channels conductances based on different sonication frequencies and intensities. We study the effect of ultrasound parameters on the firing rate, latency, and action potential amplitude of a HH neuron for a better understanding of the neuromodulation modality of ultrasound stimulation (in the continuous and pulsed modes). ConclusionsThis simulation work confirms the published experimental data that low intensity and low frequency ultrasound sonication has a higher success rate of modulating neural firing.

Active learning prediction and experimental confirmation of atomic structure and thermophysical properties for liquid Hf_{76}W_{24} refractory alloy.

The determination of liquid atomic structure and thermophysical properties is essential for investigating the physical characteristics and phase transitions of refractory alloys. However, due to the stringent experimental requirements and underdeveloped interatomic potentials, acquiring such information through experimentation or simulation remains challenging. Here, an active learning method incorporating a deep neural network was established to generate the interatomic potential of the Hf_{76}W_{24} refractory alloy. Then the achieved potential was applied to investigate the liquid atomic structure and thermophysical properties of this alloy over a wide temperature range. The simulation results revealed the distinctive bonding preferences among atoms, that is, Hf atoms exhibited a strong tendency for conspecific bonding, while W atoms preferred to form an interspecific bonding. The analysis of short-range order (SRO) in the liquid alloy revealed a significant proportion of icosahedral (ICO) and distorted ICO structures, which even exceeded 30% in the undercooled state. As temperature decreased, SRO structures demonstrated an increase in larger coordination number (CN) clusters and a decrease in smaller CNs. The alterations of the atomic structure indicated that the liquid alloy becomes more ordered, densely packed, and energetically favorable with decreasing temperature, consistent with the obtained fact: Both density and surface tension increase linearly. The simulated thermophysical properties were close to experimental values with minor deviations of 2.8% for density and 3.4% for surface tension. The consistency of the thermophysical properties further attested to the accuracy and reliability of active learning simulation.

A data-driven model of drop size prediction based on artificial neural networks using small-scale data sets

An artificial neural network (ANN) method is introduced to predict drop size in two kinds of pulsed columns with small-scale data sets. After training, the deviation between calculate and experimental results are 3.8% and 9.3%, respectively. Through ANN model, the influence of interfacial tension and pulsation intensity on the droplet diameter has been developed. Droplet size gradually increases with the increase of interfacial tension, and decreases with the increase of pulse intensity. It can be seen that the accuracy of ANN model in predicting droplet size outside the training set range is reach the same level as the accuracy of correlation obtained based on experiments within this range. For two kinds of columns, the drop size prediction deviations of ANN model are 9.6% and 18.5% and the deviations in correlations are 11% and 15%.

Responses of a Modular Floating Wind TLP of MarsVAWT Supporting a 10 MW Vertical Axis Wind Turbine

Offshore floating wind foundations supporting a large wind turbine require a large yard facility or significant facility upgrades for their fabrication. To overcome the cost increase associated with facility upgrades, an innovative lightweight modular floating foundation is developed. The foundation comprises multiple modules to enable their assembly on water, offering many benefits and expanding fabrication options for a reduction in the overall cost of the platform. In this paper, the foundation modules and their assembly are briefly described, and an analysis of the platform’s dynamic responses is presented. The modular foundation includes a modular and lightweight tension leg platform (TLP) called “MarsVAWT” which supports a Darrieus 10 MW vertical axis wind turbine (VAWT). The platform is moored with highly pretensioned wire rope tendons. The responses of the platform are analyzed in the time domain in a semi-coupled manner under the turbine operating and parked conditions for an offshore site in the US Northeast. The tower base shear forces and bending moments increase considerably with the combination of wind and waves, compared to those with wind only. The tendon tensions on the weatherside in the operating condition at high wind speeds are comparable to the values of the 50-year extreme (parked). The tendon tension increases are highly correlated to the platform pitch, as well as the horizontal and vertical velocities and vertical acceleration at the tendon porch. The modular platform performances and tendon designs are confirmed to comply with industry standards and practices.

Microfluidic Assessment of T Cell Deformability and Capillary Network Occlusion

Introduction: Adoptive T cell therapies offer a new frontier of cancer treatment that disrupts the conventional chemotherapy paradigm. Naïve T cells can be genetically modified to directly target cancerous cells and have proven to be aggressively effective in fighting cancers resistant to chemotherapies. A major challenge that adoptive T cell therapies face is successfully delivering the cell products to the location of the target cells, which is particularly challenging in the case of solid tumors. A complete characterization of the biomechanical properties of T cell products would help researchers gain insight into the capacity of these engineered cells to navigate and extravasate through vasculature and tumor microenvironments. Previous studies have found that T cell volume, cortical tension, and dynamic resistance increase with activation (Waugh et al. Front Bioeng Biotechnol. 2023). A simple and reproducible method to easily quantify and assess such cellular properties has yet to be established. Presently, we describe a microfluidic assay for the rapid and robust characterization of the mechanical changes of T cells upon activation. Our assay models the microcapillaries through a gradient of micropillar arrays, which allows for the evaluation of T cells perfused through the network. The spacing between pillars is 12 µm at the first array, similar to small arterioles, and incrementally decreases to 3 µm spacing in the last array, mimicking small capillaries ( Figure 1A). Trafficking of adoptive T cell therapies through the microvasculature networks of this scale into their target tissues represents a persistent and major challenge facing these therapies (Marofi et al. Stem Cell Res. Ther. 2021). Methods: The microfluidic devices were fabricated by casting polydimethylsiloxane (PDMS) on a silicone wafer fabricated through photolithographic protocols. Devices contain arrays of pillars measuring 20 µm x 10 µm x 12 µm with gradient spacing beginning at 12 µm at the inlet and 3 µm at the outlet. Jurkat T cells were cultured in RPMI 1640 medium with 10% fetal bovine serum and used in a naïve state or activated via antibody stimulation of CD3 and CD28. T cells were then suspended in growth media at 1x10 6 cells/mL. Microchannels were wetted and cleaned with 100% ethanol perfused at 10 µL/min for 15 minutes followed by cell culture media at 10 µL/min for 15 minutes to block the non-specific binding sites on the PDMS and to create a biocompatible environment for the cell sample. The devices were connected to a digital pneumatic pressure-based flow pump system. The cell sample was perfused at 100 mBar for 7 minutes and a subsequent wash process with growth media at 100 mBar for 7 minutes was performed to flush non-occluded cells. Upon completion of the experiment, the channel was scanned using Phase and DAPI filters with an Olympus IX83 inverted motorized microscope, and occluded T cells were quantified using ImageJ. Results: Our assay showed distinct levels of T cell occlusion upon activation through CD3 and CD28 stimulation. Naïve T cells showed lower levels of occlusion in each of the first five arrays compared to activated T cells, while both groups showed high levels of occlusion in the final 3 µm array. Activated T cells showed approximately three times the amount of occlusion in the second through fifth arrays. To assess overall occlusion, we use the Occlusion Index (OI), which considers the density of pillars and the number of cells caught in each array. We found that activated T cells had a significantly higher OI when compared to non-activated T cell samples (p < 0.05, unpaired t-test). Naïve T cells had an OI mean of 24.4 ± 5.0 while activated T cells had a mean of 42.5 ± 5.6 ( Figure 1B). Conclusions: Our results show that there is a significant increase in resistance of activated T cells through a microvasculature-modeled environment compared to their naïve counterparts. As adoptive T cell therapies increase in popularity, a complete understanding of the biomechanical properties of these cell products will be necessary for effective design. Our microfluidic assay mimics the narrowing channels of the microvasculature and permits the study of how naïve and engineered T cells travel through these mechanically challenging spaces.