Increase In Starch Content Research Articles

Evaluation of spring barley cultivars released in Belarus under the environmental conditions of the Northern Trans-Urals

Background. Studying the genetic diversity of ex situ barley accessions under contrasting environmental conditions (humid with a lack of heat, favorable, arid, etc.) makes it possible to assess them according to the duration of the growing season, resistance to lodging, potential productivity, drought resistance, grain quality, and environmental plasticity. Materials and methods. The results of an 8-year trial (2016–2023) of 11 spring barley accessions of Belarusian origin in the northern forest-steppe zone of Tyumen Province are presented. The experiments followed the protocols adopted for the state variety trials. An SKS-6-10 seeder was used for sowing, the plot area was 5 m2 , and cv. ʻAbalakʼ served as the reference. The seeding rate was 550 viable seeds per 1 m2 . Results. A majority of the studied accessions matured later than the medium-ripening reference cultivar. Weather conditions during the growing season in the Northern Trans-Urals had a decisive effect on the harvest formation, protein content and starch content in barley grain (74.6%, 70.8% and 81.8%, respectively). The effect of the genotype on these indicators was 7.9%, 17.5% and 6.2%, respectively. Starch content also depended on the genotype × environment interaction (9.7%). Fat content was almost equally affected by the genotype (25.1%) and the environment (26.7%). The genotype × environment interaction was more significant (31.2%). Conclusion. The following sources were identified among the tested cultivars: ‘Vodar’ (high yield, drought tolerance, etc.); ‘Magutny’ (high yield, and environmental plasticity); ‘Khago’ (large grain, low hull content, and increased protein and starch content levels); ‘Lipen’ (high test weight, increased starch content, and environmental plasticity); ‘Fobos’ (drought tolerance, and increased protein content); ‘Dublet’ (lodging resistance, and increased protein content); ‘Taler’ (reduced protein content, and environmental plasticity); ‘Pospekh’ (reduced protein content).

Cassava, corn, wheat, and sweet potato native starches: A promising biopolymer in the production of capsules by electrospraying.

Native starches have a high potential for producing capsules by electrospraying despite still being little explored as biopolymeric material. Thus, the present study aimed to investigate the electrospraying capacity of native starches from different sources (cassava, corn, wheat, and sweet potato). The concentration of starch in the polymer solutions was varied from 3% to 10% (w/v) to investigate the impact of both starch source and concentration on the electrospraying process. The study also aimed to deepen scientific knowledge of the behavior of these starches during the process and the characteristics of the resulting capsules. The characterization of the polymeric solutions was carried out regarding rheology and electrical conductivity and the capsules were evaluated by production yield, morphology, size distribution, thermal properties, and crystallinity. Higher viscosities were observed for polymeric solutions obtained from sweet potato starch, compared to other starches at the same polymer concentrations. The increase in starch concentration in polymeric solutions reduced their electrical conductivity, except for corn starch. The production yield of cassava and sweet potato starch capsules was not affected by the polymer concentration in the polymeric solutions, while for corn and wheat starches, there was a reduction in yield as the concentration increased. All starches were able to form capsules. Capsules produced with 7% corn starch showed a more homogeneous size distribution. The electrosprayed starches showed elevated melting temperatures and enthalpies. The capsules produced had an amorphous structure. The starches evaluated in this study proved to be excellent biopolymers for producing capsules by electrospraying, demonstrating possible future uses as encapsulating materials.

Potassium indole-3-butyric acid affects rice's adaptability to salt stress by regulating carbon metabolism, transcription factor genes expression, and biosynthesis of secondary metabolites.

Soil salinity pollution is increasing worldwide, seriously affecting plant growth and crop production. Existing reports on how potassium indole-3-butyric acid (IBAK) regulates rice salt stress adaptation by affecting rice carbon metabolism, transcription factor (TF) genes expression, and biosynthesis of secondary metabolites still have limitations. In this study, an IBAK solution at 40 mg L-1 was sprayed on rice leaves at the seedling stage. The results showed that the IBAK application could promote shoot and root growth, decrease sucrose and fructose content, increase starch content, and enhance acid invertase (AI) and neutral invertase (NI) activity under salt stress, indicating altered carbon allocation. Furthermore, the expression of TF genes belonging to the ethylene responsive factor (ERF), WRKY, and basic helix-loop-helix (bHLH) families was influenced by IBAK. Many key genes (OsSSIIc, OsSHM1, and OsPPDKB) and metabolites (2-oxoglutaric acid, fumaric acid, and succinic acid) were upregulated in the carbon metabolism pathway. In addition, this study highlighted the role of IBAK in regulating the biosynthesis of secondary metabolites pathway, potentially contributing to rice stress adaptability. The results of this study can provide new sustainable development solutions for agricultural production.

A glutathione S-transferase IbGSTL2 interacts with IbcPGM to increase starch content and improve starch quality in sweetpotato

A glutathione S-transferase (GST) gene IbGSTL2 was cloned and characterized from sweetpotato. It harbored a variation associated with starch content in storage roots. Overexpression of IbGSTL2 increased starch content and amylopectin proportion, decreased gelatinization temperature, and improved degree of crystallinity in sweetpotato storage roots, while its RNA interference resulted in the opposite trends. IbGSTL2 physically interacted with IbcPGM, an enzyme of sucrose metabolism, and improve starch content and quality by regulating genes involved in starch biosynthesis.

SlNAP1 promotes tomato fruit ripening by regulating carbohydrate metabolism

Many studies showed NAC transcription factors play an important role in fruit ripening. Moreover, sucrose and starch metabolism is also closely related to fruit ripening. However, there are a few studies focus on whether NAC regulates sucrose and starch metabolism to influence fruit ripening. In this study, virus-induced gene silencing (VIGS) of SlNAP1 suppressed fruit ripening and delayed color transformation. The chlorophyll (including Chla, Chlb, and Chla + b) degradation and carotenoid synthesis in SlNAP1-silenced fruits were dramatically suppressed. Silencing SlNAP1 decreased soluble sugar and reducing sugar accumulation in fruits, and increased starch content. The activity of starch degrading enzymes, including α amylase (AMY) and β amylase (BAM) was significantly lower in SlNAP1-silenced fruits than in the control fruits, whereas denosine diphosphoglucose pyrophosphorylase (AGP) activity was significantly higher. In addition, the expression of starch degradation-related genes (SlAMY1, SlAMY2, SlBAM1, SlBAM7, SlGWD, SlPWD) in SlNAP1-silenced fruits was significantly suppressed, while starch synthesis-related genes (SlAGPase1, SlAGPase2) was significantly increased. Compared with the control fruits, SlNAP1-silenced fruits showed significantly lower sucrose and glucose content. The expression level of sucrose and glucose metabolism-related genes such as Slsus1, Slsus3, SlSPS, SlHxk1, SlHxk2, SlPK1, and SlPK2 was significantly lower in SlNAP1-silenced fruits than in the control fruits. Overall, this study revealed that SlNAP1 gene might positively regulate fruit ripening by influencing carbohydrate metabolism.

Dry Matter Accumulation, Water Productivity and Quality of Potato in Response to Regulated Deficit Irrigation in a Desert Oasis Region.

As one of the most important food crops, the potato is widely planted in the oasis agricultural region of Northwest China. To ascertain the impact of regulated deficit irrigation (RDI) on various facets including dry matter accumulation, tuber yield, quality and water use efficiency (WUE) of potato plants, a two-growth season field experiment under mulched drip irrigation was conducted in the desert oasis region of Northwest China. Water deficits, applied at the seedling, tuber formation, tuber expansion and starch accumulation stages, encompassed two distinctive levels: mild (55-65% of field capacity, FC) and moderate (45-55% FC) deficit, with full irrigation (65-75% FC) throughout the growing season as the control (CK). The results showed that water deficit significantly reduced (p < 0.05) above-ground dry matter, water consumption and tuber yield compared to CK, and the reduction increased with the increasing water deficit. A mild water deficit at the tuber formation stage, without significantly reducing (p > 0.05) yield, could significantly increase WUE and irrigation water use efficiency (IWUE), with two-year average increases of 25.55% and 32.33%, respectively, compared to CK. Water deficit at the tuber formation stage increased starch content, whereas water deficit at tuber expansion stage significantly reduced starch, protein and reducing sugar content. Additionally, a comprehensive evaluation showed that a mild water deficit at the tuber formation stage is the optimal RDI strategy for potato production, providing a good balance between yield, quality and WUE. The results of this study can provide theoretical support for efficient and sustainable potato production in the desert oasis regions of Northwest China.

Effect of Different Fertilizer Types on Quality of Foxtail Millet under Low Nitrogen Conditions.

In order to clarify the effect of different fertilizers on foxtail millet quality under low nitrogen conditions, we used JGNo.21 and LZGNo.2 as experimental materials and set up five treatments, including non-fertilization, nitrogen, phosphorus, compound, and organic fertilizers, to study the regulation of different fertilizer types on agronomic traits, nutrient fractions, and pasting characteristics of foxtail millet under low nitrogen conditions. Compared with the control, all of the fertilizers improved the agronomic traits of JGNo.21 to a certain extent. Nitrogen and compound fertilizer treatments reduced the starch content of JGNo.21; the starch content was reduced by 0.55% and 0.07% under nitrogen and compound fertilizers treatments. Phosphorus and organic fertilizers increased starch content, and starch content increased by 0.50% and 0.56% under phosphorus and organic fertilizer treatments. The effect of each fertilizer treatment on protein content was completely opposite to that of starch; different fertilizer treatments reduced the fat content of JGNo.21 and increased the fiber content. Among them, nitrogen and phosphorus fertilizers increased the yellow pigment content; the yellow pigment content increased by 1.21% and 2.64% under nitrogen and phosphorus fertilizer treatments. Organic and compound fertilizers reduced the content of yellow pigment; the yellow pigment content was reduced by 3.36% and 2.79% under organic and compound fertilizer treatments. Nitrogen and organic fertilizers increased the fat content of LZGNo.2; the fat content increased by 2.62% and 1.98% under nitrogen, organic fertilizer treatment. Compound and phosphorus fertilizer decreased the fat content; the fat content decreased by 2.16% and 2.90% under compound and phosphorus fertilizer treatment. Different fertilizer treatments reduced the cellulose and yellow pigment content of LZGNo.2. The content of essential, non-essential, and total amino acids of JGNo.21 was increased under compound and nitrogen fertilizer treatments and decreased under organic and phosphorus fertilizer treatments. The content of essential, non-essential, and total amino acids of LZGNo.2 was significantly higher under compound, nitrogen, and organic fertilizer treatments compared with control and significantly decreased under phosphorus fertilizer treatments. Nitrogen and compound fertilizer treatments significantly reduced the values of peak viscosity, trough viscosity, breakdown viscosity, final viscosity, setback viscosity, and pasting time of each index of JGNo.21; phosphorus and organic fertilizer treatments improved the values of each index. In contrast, the pasting viscosity of LZGNo.2 increased under phosphorus fertilizer treatment and decreased under nitrogen fertilizer treatment. Reasonable fertilization can improve the quality of foxtail millet, which provides a scientific theoretical basis for improving the quality of foxtail millet.

PSIII-16 Effects of dietary corn origin (U.S., Argentina, and Brazil) on pig growth performance

Abstract Corn is an important global commodity. Understanding its nutritional value and practically utilizing different corn sources and varieties in swine diets is important to U.S. corn and swine production. Globally, the U.S. “dent” corn variety is presumed to be of lesser quality compared with “harder” flint-type corn (the preferred varieties in Argentina and Brazil) due to differences in whole kernel structure when shipped long distances. In fact, official work from the US Grains Council indicates that US corn produces more ethanol per bushel when compared with flint-type varieties of corn. This indicates a greater concentration of starch in US corn and increased starch content should mean a greater quantity of energy and nutrients. Therefore, swine producers globally benefit from understanding the feeding value of different varieties and origins of corn. The objective of this study was to assess the performance of pigs fed corn from three exporting countries: U.S., Argentina (ARG), and Brazil (BRZ). Two similar studies were conducted comparing the effect of 3 corn sources on late nursery pig growth performance. Study 1 used 96 nursery pigs [body weight (BW) = 8.8 ± 1.7 kg] split between 24 pens and study 2 used 114 nursery pigs (8.0 ± 1.3 kg) split between 24 pens. Three experimental diets were formulated with corn sources from: 1) U.S.; 2) ARG; and, 3) BRZ. The U.S. corn diet was considered the control diet and was balanced to meet or exceed all nutrient requirements for the pigs. The U.S. corn was replaced with ARG corn and BRZ corn on a kg for kg basis in the diets. Pigs were assigned in a randomized block design based on initial body weights (BW) and sex. Feed and water were offered ad libitum over a 28-d period, pig BW and feed disappearance were measured each week. Average daily gain (ADG), average daily feed intake (ADFI), and feed efficiency [gain to feed ratio (G:F)] were determined after weeks 1, 2, and 4. Performance data were analyzed using PROC MIXED procedure in SAS considering dietary treatment as a main effect and pen as experimental unit. There were no significant differences (P &amp;gt; 0.05) in BW, ADFI, ADG, and feed efficiency between the three corn sources throughout all weeks and the overall 28 d. Overall performance values for the three treatments were: US corn ADG (0.66 kg), ADFI (0.91 kg), and G:F (0.72): Argentina corn, ADG (0.65 kg), ADFI (0.92), and G:F (0.70); and Brazil corn, ADG (0.65), ADFI (0.92), and G:F (0.71). In conclusion, the value of US corn is similar in nutrient content and nutritional quality as demonstrated by equivalent performance in nursery pigs. The aesthetics of whole kernels are not indicative of feeding value to swine.

Acetylation proteomics and metabolomics analyses reveal the involvement of starch synthase undergoing acetylation modification during UV-B stress resistance in Rhododendron Chrysanthum Pall

BackgroundRhododendron chrysanthum Pall. (R. chrysanthum) is a plant that lives in high mountain with strong UV-B radiation, so R. chrysanthum possess resistance to UV-B radiation. The process of stress resistance in plants is closely related to metabolism. Lysine acetylation is an important post-translational modification, and this modification process is involved in a variety of biological processes, and affected the expression of enzymes in metabolic processes. However, little is known about acetylation proteomics during UV-B stress resistance in R. chrysanthum.ResultsIn this study, R. chrysanthum OJIP curves indicated that UV-B stress damaged the receptor side of the PSII reaction center, with a decrease in photosynthesis, a decrease in sucrose content and an increase in starch content. A total of 807 differentially expressed proteins, 685 differentially acetylated proteins and 945 acetylation sites were identified by quantitative proteomic and acetylation modification histological analysis. According to COG and subcellular location analyses, DEPs with post-translational modification of proteins and carbohydrate metabolism had important roles in resistance to UV-B stress and DEPs were concentrated in chloroplasts. KEGG analyses showed that DEPs were enriched in starch and sucrose metabolic pathways. Analysis of acetylation modification histology showed that the enzymes in the starch and sucrose metabolic pathways underwent acetylation modification and the modification levels were up-regulated. Further analysis showed that only GBSS and SSGBSS changed to DEPs after undergoing acetylation modification. Metabolomics analyses showed that the metabolite content of starch and sucrose metabolism in R. chrysanthum under UV-B stress.ConclusionsDecreased photosynthesis in R. chrysanthum under UV-B stress, which in turn affects starch and sucrose metabolism. In starch synthesis, GBSS undergoes acetylation modification and the level is upregulated, promotes starch synthesis, making R. chrysanthum resistant to UV-B stress.

Studies on Physicochemical Properties of Rice Bean (Vigna umbellata) Starch: An Underutilized Legume

Starch is a reserve carbohydrate present in plants. It is an important component for maintaining various quality attributes like texture, consistency, thickening in the food industry. The aim of the present study was to isolate starch from a legume named jack bean that is not very much famous and considered as underutilized-legume. The isolated starch was studied for various physicochemical properties. One-way analysis of variance was used in this study. The starch was isolated as a white powder from the dry legume by using standard method and the starch yield was observed to be 25.79%. Legume starch was further assessed for the chemical composition. The moisture, ash, fat, protein, fiber and carbohydrate content were 9.56%, 0.35%, 0.21%, 0.69%, 0.33%, and 89.19% respectively. The apparent and total amylose content was 37.62% and 40.83% with least gelation concentration at 6% of starch gel. Starch was further assessed for functional properties. The leached amylose displayed a rise in content from 6.56% to 9.50% with the corresponding increase in temperature from 65°C to 95°C that may affect gelatinization properties of starch. The water absorption capacity was 3.60 g/g while oil absorption capacity was 3.54 g/g. Further, the emulsion stability and emulsion capacity of native starch was 79.00% and 68.33% respectively that showed good stability of emulsion. Similarly, the swelling power and solubility of rice bean starch increased with the increase of temperature from 55-95°C. However, the opposite trend was observed for an increase in starch concentration from 1-4%. The effect of different concentrations at 6, 8 and 10% on freeze-thaw stability and storage studies showed that the expelled water decreased with the rise in starch concentration up to three consecutive storage days while no change was observed later during frozen gel storage. It showed good freeze thaw stability of starch. This underutilized legume attracted more attention from researchers due of its affordability and ease of availability. In dry farming circumstances with limited soil fertility, the legume yield consistently. Thus, this neglected legume that has minimal agronomic development but many qualities is a good substitute for starch. However, thorough study on the unique functional properties and other qualities of starch from rice bean has not been carried out. The results of this underutilized legume starch can provide opportunities to starch researchers and to the food industries that are frequently using conventional sources of starch from cereals, tubers and rhizomes.

Enhanced starch accumulation in Chlorella sorokiniana as sugar platform and the expression profiling of key regulatory proteins

Microalgae can accumulate considerable starch as a renewable sugar platform for biofuel production. In this study, Chlorella sorokiniana exhibited a significant increase in starch content from 3.0% to 49.5% DM under sulfur limitation (SL) conditions, and the lower the concentration of sulfur was, the more effective it was. Direct enzymatic hydrolysis of wet biomass resulted in a glucose yield of 97.1%, indicating its potential for fermentable sugar production. However, C. sorokiniana grew slowly, and cell division and biomass accumulation decreased under SL conditions, which could be explained by the decreased photosynthetic efficiency and protein content. The sulfur replenishment (SR) confirmed the rapid response of C. sorokiniana in starch accumulation to sulfur availability. Thus, we further investigated the expression profiling of key regulatory proteins under Control-SL-SR stages. The expression of AGPase A was strongly induced in the SL stage and subsequently downregulated during SR stage, suggesting its role as a key regulatory protein for enhanced starch accumulation. Additionally, downregulation of SBE and GBE expression likely contributed to structural modifications of the starch molecules. These proteins could serve as potential targets for metabolic engineering in further improving the starch production capacity in C. sorokiniana.

The Effect of Lotus Root Starch Content on the Water Barrier Properties and Biodegradability of Cassava Bioplastic Films

Biodegradable films demand increases due to the awareness of the environmental effects of synthetic plastics. However, biodegradable films based on starch have high water sensibility and poor mechanical properties. This led to an interest among the scientist in improving the properties of biodegradable films. The objectives of this study were to investigate the effect of lotus root starch content on the water solubility, water absorption, water vapor permeability and biodegradability of cassava bioplastic films. The lotus root starch was added at 10%, 20%, 30%, 40% and 50% of cassava starch. The results showed that the water absorption properties decreased by 122% to 21% and the water vapor permeability showed a decreasing trend as the lotus starch content increased. The water solubility increased from 4% to 36% with the increase of lotus starch content and biodegradability increased by 87.5% at 50% of lotus starch content. The results exposed the potential of cassava/ lotus starch bioplastic films for food packaging applications.

Plant growth regulators improve the growth and physiology of transplanted Thalassia Hemprichii fragments

IntroductionThe transplantation of seagrass fragments with shoots and rhizomes is the most common method for the ecological restoration of damaged seagrass meadows.MethodsThe aim of this study was to explore the effects of exogenous addition (10 mg per month for 3 months) of three commonly used plant growth regulators (PGRs), including indoleacetic acid (IAA), gibberellin (GA3), and paclobutrazol (PP333) on the growth and physiology of transplanted Thalassia hemprichii fragments (TTFs), with and without the rhizome apex (RA), using a simulation test. [Results] IAA and GA3 elevated the photosynthetic capacity and growth rate of TTF leaves but did not significantly alter leaf length and width. In contrast, PP333 reduced the leaf photosynthetic capacity and growth rate, while greatly increasing the leaf width and root viability. Additionally, PP333 treatment led to an increase in antioxidant enzyme activities (peroxidase [POD], superoxide dismutase [SOD], and catalase [CAT]), as well as malondialdehyde (MDA) and total phenol contents in TTFs, indicating some stress effects on the seagrass. Furthermore, IAA and GA3 decreased soluble sugar and protein contents and increased starch content in TTF tissues, whereas PP333 treatment elevated both nonstructural carbohydrate and soluble protein contents. The presence of RA positively affected the growth and physiology of T. hemprichii compared with TTFs without RA. There was a significant interaction between the PGRs and RA on leaf growth rate, chlorophyll fluorescence variables, and photosynthetic pigment content of the TTFs (p &amp;lt; 0.05).DiscussionThis study provides guidelines for the application of PGRs during the transplantation of T. hemprichii fragments for the restoration of seagrass meadows.

Effectiveness of applying the preparation Tropikanka 1 on potatoes in the forest steppe of the Obie region

The results of complex experiments to study the effectiveness of using the drug Tropikanka 1 on early potatoes of the Vega variety are presented. The studies were conducted in arid conditions on grey forest soils of the northern forest-steppe of the Novosibirsk Ob region. Before planting, the tubers were soaked for 15 minutes in a solution of Tropikanka 1 with a dilution of 1:50 depending on the options: 1:100, 1:200 and without using the drug with soaking in water (control). The research aims to develop methods for using the environmentally friendly organic fertiliser Tropikanka 1 to stimulate the growth and development of potatoes, increasing their yield and product quality. It was established that on grey forest heavy loamy soil of the forest-steppe of the Novosibirsk Ob region under conditions of acute moisture deficiency in May, June and early July 2023, the use of pre-planting soaking of early potato tubers of the Vega variety for 15 minutes in a solution of Tropikanka 1 fertiliser, obtained based on bird droppings, accelerated the growth and development of potatoes by seven days compared to the control (using water). The largest area of potato leaves was observed against the background of pre-planting treatment of tubers with Tropikanka 1 at a dilution of 1:100, exceeding the control by 1.8 times. The yield of early potato production (the beginning of the third decade of July) with a dilution of the drug 1: 100 is 1.7 times higher than the control. The total yield against the background of soaking tubers with Tropikanka 1 fertiliser for the Vega variety when diluting the drug 1: 100 reached up to 33.8 t/ha, which is 38% higher than the control. At the same time, the yield of marketable products increased to 30 t/ha, exceeding the control by 52 %. The quality indicatorsof commercial products also improved. When the drug was diluted 1:100, there was an increase in starch content by 0.2 %. The concentration of nitrates during the use of the medication is 6.5 times lower than the maximum permissible concentration for potatoes.

THE RESULTS OF BREEDING THE SELECTION-VALUABLE POTATO HYBRIDS BY THE METHOD OF STEPWISE INTERVARIETAL HYBRIDIZATION

To implement programs of applied potato breeding at Lviv NЕU, when creating the initial breeding material, the staged intervarietal crossings were carried out. Its idea was to combine a complex of valuable economic traits that were inherent in the original parental forms into one genotype. As a result of the stepwise intervarietal hybridization in Lviv NЕU, several new hybrid forms of potatoes of different origins and maturing periods were created. The original parental forms for hybridization used potato varieties of Ukrainian selection, namely Borodianska Rozheva, Vodohrai, Hibrydna 14, Volia, Zakhidna, Zov, Lishchyna, Lvivianka, Oksamyt, Povin, Svitanok Kyivskyi, and foreign varieties, such as Nevskа, Suzorye, Aminca, Sante, hybrid SVP. The task of the research was to give a comprehensive assessment of promising intervarietal hybrids of potatoes according to their valuable economic and biological characteristics so that the best forms could be recommended as candidates for new varieties. The research was conducted during 2020–2022. 9 new potato hybrids of different maturity groups were used for the studies. Each of the hybrids and the corresponding standard varieties of potatoes were planted on land plots with a plant feeding area of 70×35 cm, which amounted to 40.8 thousand plants per 1ha. The experimental sites were placed in triplicate, using a systematic method. The registered area of the site was 25 m2. Potato varieties of domestic selection were used as standards, in particular, Vodohrai variety – for the mid-early group, Volia variety - for mid-ripening, and Zakhidna – for mid-late. It is established that the stepwise intervarietal hybridization allows combining potentially high productivity (1000g/bush and higher), intensive crop formation with increased starch content in tubers (16–18%), and high resistance to late blight (at the level of 7–8 points). Basing on the conducted research of the comprehensive evaluation of the economic and biological characteristics of new intervarietal hybrids of potatoes, the mid-early hybrid 02/10-40 (Borodianska rozheva × Suzorye), the mid-ripening 11/4-1 (Zakhidna × Vodohrai) and the mid-late hybrid 16/17-1 [(Zakhidna × Sante) × (Volia × Lishchyna)] were marked as they significantly prevailed in the complex of economic and valuable characteristics of the corresponding standard varieties. It is proposed to continue further selection work with them according to the accepted schemes and potato selection, intensively multiply, and prepare to pass to the State variety tests as candidates for new varieties of the crop.

Exogenous abscisic acid improves grain filling capacity under heat stress by enhancing antioxidative defense capability in rice

BackgroundHeat stress is a major restrictive factor that causes yield loss in rice. We previously reported the priming effect of abscisic acid (ABA) on rice for enhanced thermotolerance at the germination, seedling and heading stages. In the present study, we aimed to understand the priming effect and mechanism of ABA on grain filling capacity in rice under heat stress.ResultsRice plants were pretreated with distilled water, 50 μM ABA and 10 μM fluridone by leaf spraying at 8 d or 15 d after initial heading (AIH) stage and then were subjected to heat stress conditions of 38 °C day/30 °C night for 7 days, respectively. Exogenous ABA pretreatment significantly super-activated the ABA signaling pathway and improved the SOD, POD, CAT and APX enzyme activity levels, as well as upregulated the ROS-scavenging genes; and decreased the heat stress-induced ROS content (O2– and H2O2) by 15.0–25.5% in rice grain under heat stress. ABA pretreatment also increased starch synthetase activities in rice grain under heat stress. Furthermore, ABA pretreatment significantly improved yield component indices and grain yield by 14.4–16.5% under heat stress. ABA pretreatment improved the milling quality and the quality of appearance and decreased the incidence of chalky kernels and chalkiness in rice grain and improved the rice grain cooking quality by improving starch content and gel consistence and decreasing the amylose percentage under heat stress. The application of paraquat caused overaccumulation of ROS, decreased starch synthetase activities and ultimately decreased starch content and grain yield. Exogenous antioxidants decreased ROS overaccumulation and increased starch content and grain yield under heat stress.ConclusionTaken together, these results suggest that exogenous ABA has a potential priming effect for enhancing rice grain filling capacity under heat stress at grain filling stage mainly by inhibiting ROS overaccumulation and improving starch synthetase activities in rice grain.

Transformation of banana peel into biodegradable film added with starch and carboxymethyl cellulose and its characterization

Synthetic polymers are non-biodegradable in nature and lead to serious environmental as well as health problems. A biodegradable material can be a good replacer for commercial Synthetic plastics. The development of banana peel-based biodegradable film is a good source to utilize food processing waste. The biodegradable film was prepared using banana peel paste (BPP), corn starch (0%, 0.5%, 1.0%, 1.5%, 2.0% w/w of BPP), carboxymethyl cellulose sodium salt (15% of corn starch) and glycerol as a plasticizer. The films were developed by casting the suspension on petri dishes fallowed by drying. The mechanical, thermal, and barrier characteristics of dried films were characterized. The films moisture content, thickness, WVP, solubility, tensile strength, elongation, and biodegradability were in the range of 31.807–33.241%, 0.211–0.318 mm, 1.668–2.948 g mm/d/m2/kPa, 31.269–36.105 %, 0.825–1.158 MPa, 18.976–25.516 %, 65.034–70.002 % respectively. The film with 0.5 % of starch has been found to enhance the tensile strength (1.158 MPa). With an increase in starch content, the film becomes more permeable to water vapor and more soluble. The film was successfully developed and has good biodegradability and acceptable properties. Banana peel-based films might become a significant player in the food packaging industry in the future, helping to reduce the usage of paper and plastic bags.

Effect of particle size classification on properties of flour from jack bean: An under-utilized high protein legumes

Jack bean (Canavalia ensiformis L., DC) is one of the most under-utilized crops in the food industry. This study explored the chemical and functional properties of whole seed flour (WJB) and kernel flour (KJB). The impact of dry sieving for particle size classification on WJB and KJB flours was also investigated. Unclassified WJB and KJB exhibit protein contents of 30.7 ± 0.2 g/100 g and 35.2 ± 0.1 g/100 g1, respectively, along with a resistant starch content of 5.8 ± 0.1 g/100 g and 4.6 ± 0.1 g/100 g, respectively. The protein in jack bean flour compares favorably with beef and egg protein in terms of amino acid composition. The crystallinity, swelling power, solubility, and pasting properties of WJB and KJB flours tended to increase with particle size reduction, corresponding with an increase in starch content. This study highlights that dry sieving is an effective technique for diversifying the chemical and functional qualities of jack bean flour. Smaller sieve apertures selectively remove non-starch components, allowing for tailored flour properties.

Comparative study on the in vitro digestion of different lipids in starch-based Pickering emulsions

Starch-based Pickering stabilizer has attracted more attentions due to its health-friendly attribute. Lipid digestion in Pickering emulsion is the key to its delivery ability for active substances. Herein, in vitro oral-gastric-intestinal digestions of Pickering emulsions stabilized by starch particles with different oil phases (e.g., coconut, corn, olive, and sunflower oils) were investigated. The highest rate of lipid digestion was coconut oil (25.71 %), followed by olive (12.64 %), corn (11.16 %), and sunflower (8.99 %) oils. The lipid digestibility was influenced by saturation of fatty acids: coconut (91.41 %)>olive (16.58 %)>corn (14.63 %)>sunflower (10.85 %) oils. The increase of starch concentration (0.5 % – 4.0 %, w/w) had positive effects, while the increase of oil fraction (25 % – 70 %, v/v) had negative impacts on free fatty acid release due to the formation of different initial droplet sizes. The microstructures observed using confocal laser scanning microscope indicated that starch-based Pickering emulsion possessed super stability against oral and gastric digestions, which made it a superior delivery system for lipophilic active substances under severe gastric environment. These results may promote the design of functional food emulsions stabilized by starch particles which can regulate digestion of triglycerides.

Potential 3D filament of Janeng (Dioscorea hispida Dennst) starch: Preparation and characterization

The preparation and characterization of filaments from janeng have been studied. The janeng filament (PCl-JS) process starts with the preparation and gelatinization of janeng starch, and then the resulting starch gelatinization, which is usually called thermoplastically processable starch (TPS) is carried out by filament processing by mixing PCl-g-GMA and JS at a temperature of 170 °C and 80 rpm for 15 min. The prepared filaments were analyzed. SEM studies show that the fracture surface of PCl-JS0 filaments provides a uniform fiber dispersion, in contrast to the morphology showing large and interconnected starch domains as the starch concentration increases from 1% to 9%. The intensity of hydrogen bonds observed in the spectrum via FTIR increased in band as the concentration of JS increased. XRD analysis observed that the crystallinity and crystal size of filaments without starch were higher than those of other PCl filaments that had undergone modification with starch. Mechanical analysis showed that starch did not have a negative effect on the tensile strength of the filaments, indicating good compatibility between JS and the PCl matrix. When the additional concentration of JS increased to 9%, the strength of PCl-JS filaments increased to 37 MPa, which was caused by the higher crystallinity induced by starch. The glass temperature of the filament sample was observed using DSC, which showed that PCl without JS occurred at 48.2 °C and shifted (+2 °C) towards 49.4 °C when JS was added. For all DSC curves, the exothermic peak was observed around 56–59 °C. The melt flow behavior of PCl-JS filaments decrease with increasing starch content.