A numerical investigation is carried out to understand the effects of thermal buoyancy and Reynolds number on flow characteristics and mixed convection heat transfer over three isothermal circular cylinders situated in a tandem arrangement within a horizontal channel. The distance between cylinders is fixed at the value of 2.5 widths of the cylinder. The obtained results are presented and discussed for the range of conditions as: Re = 5 to 40, Ri = 0 to 2 at fixed Pr number of 1 and blockage ratio β = 0.25. The main results are depicted in terms of streamlines and isotherm contours to analyze the effect of thermal buoyancy on fluid flow and heat transfer rate. Moreover, the overall drag coefficient and Nusselt number are computed to elucidate the role of Reynolds number and Richardson number on the flow and heat transfer. It is found that increase in the Richardson number increases the drag coefficient of the upstream cylinder whereas it decreases the heat transfer rate of this cylinder. The superimposed of thermal buoyancy created a new sort of recirculation zones between the tandem cylinders.