Increased Nutrient Inputs Research Articles

Microbial expression profiles in the rhizosphere of willows depend on soil contamination.

The goal of phytoremediation is to use plants to immobilize, extract or degrade organic and inorganic pollutants. In the case of organic contaminants, plants essentially act indirectly through the stimulation of rhizosphere microorganisms. A detailed understanding of the effect plants have on the activities of rhizosphere microorganisms could help optimize phytoremediation systems and enhance their use. In this study, willows were planted in contaminated and non-contaminated soils in a greenhouse, and the active microbial communities and the expression of functional genes in the rhizosphere and bulk soil were compared. Ion Torrent sequencing of 16S rRNA and Illumina sequencing of mRNA were performed. Genes related to carbon and amino-acid uptake and utilization were upregulated in the willow rhizosphere, providing indirect evidence of the compositional content of the root exudates. Related to this increased nutrient input, several microbial taxa showed a significant increase in activity in the rhizosphere. The extent of the rhizosphere stimulation varied markedly with soil contamination levels. The combined selective pressure of contaminants and rhizosphere resulted in higher expression of genes related to competition (antibiotic resistance and biofilm formation) in the contaminated rhizosphere. Genes related to hydrocarbon degradation were generally more expressed in contaminated soils, but the exact complement of genes induced was different for bulk and rhizosphere soils. Together, these results provide an unprecedented view of microbial gene expression in the plant rhizosphere during phytoremediation.

Rapid changes in the redox conditions of the western Tethys Ocean during the early Aptian oceanic anoxic event

The early Aptian (125 to 121Ma) records an episode of severe environmental change including a major perturbation of the carbon cycle, an oceanic anoxic event (OAE 1a, 122.5Ma), a platform drowning episode and a biocalcification crisis. We propose to trace changes in the oxygenation state of the ocean during the early Aptian anoxic event using the redox-sensitive trace-element (RSTE) distribution, phosphorus accumulation rates (PARs) and organic-matter characterization in three different basins of the western Tethys. The following sections have been investigated: Gorgo a Cerbara (central Italy) in the Umbria Marche basin, Glaise (SE France) in the Vocontian basin and Cassis/La Bédoule (SE France) located in the Provencal basin.In the Gorgo a Cerbara section, RSTE distributions show a low background level along the main part of the section, contrasted by different maxima in concentrations within the Selli level. In the Glaise section, the Goguel level displays a weak increase in RSTE contents coeval with moderate TOC values. At Cassis/La Bédoule, no significant RSTE enrichments have been observed in sediments equivalent to the Selli level. These differences in the records of the geochemical proxies of the Selli level or its equivalent indicate the deposition under different redox conditions, probably related to the paleogeography. Our data indicate the development of anoxic–euxinic conditions in the deeper part of the Tethys during OAE 1a, whereas in the shallower environments, conditions were less reducing. Moreover, at Gorgo a Cerbara, the Selli level is characterized by rapid changes in the intensity of reducing conditions in the water column. Ocean eutrophication seems to be a major factor in the development and the persistence of anoxia as suggested by the PAR evolution. Higher PAR values at the onset of OAE 1a suggest an increase in nutrient input, whereas the return to lower values through the first part of the OAE 1a interval may be related to the weakened capacity to retain P in the sedimentary reservoir due to bottom-water oxygen depletion. This general pattern is contrasted by the data of Gorgo a Cerbara, where the sediments deposited during the OAE 1a interval show P-enrichments (mainly authigenic P). This is associated with maxima in TOC values and Corg:Ptot ratios, suggesting that a part of the remobilized P was trapped in the sediments and as such prevented from returning to the water column.

White‐tailed deer (Odocoileus virginianus) positively affect the growth of mature northern red oak (Quercus rubra) trees

Understanding and predicting the effects of deer (Cervidae) on forest ecosystems present significant challenges in ecosystem ecology. Deer herbivory can cause large changes in the biomass and species composition of forest understory plant communities, including early life‐cycle trees (i.e., seedlings and saplings). Such changes can impact juvenile to adult transitions and the future age structure and species composition of mature forests. Changes to understory vegetation also impact flow of energy and nutrients in forest ecosystems. Studies examining the influence of deer on mature trees, however, are rare and rely on extrapolating effects from early life cycle stages of trees. We tested the hypothesis that the absence of deer would result in an increase in the growth rate of mature trees by examining the impact of white‐tailed deer (Odocoileus virginianus) on mature canopy trees. We examined incremental growth in individuals of Quercus rubra, an important component of temperate deciduous forests in North America, inside and outside 16‐year deer exclosures in eastern U.S. deciduous forests. We found that adult trees inside exclosures grew less than those directly exposed to deer. Our findings highlight the indirect effects of white‐tailed deer on the growth of adult individuals of Q. rubra in a way opposite of what would be expected from previous studies based on immature or understory tree populations. We suggest the increased growth of adult trees in the presence of deer may be explained by increased nutrient inputs through deer fecal and urine deposits and the alteration of the competitive environment belowground through the reduction of understory vegetation by browsing. Underscoring the ecological and demographic importance of adult trees in forest ecosystems, results from this study suggest the direct and indirect effects of deer on mature trees should not be overlooked.

The middle Rhuddanian (lower Silurian) ‘hot’ shale of North Africa and Arabia: An atypical hydrocarbon source rock

An important hydrocarbon source rock (a ‘hot’ shale) has been identified from the middle Rhuddanian (Llandovery, lower Silurian) of a number of localities in North Africa and Arabia. It is unusual in having been deposited during a eustatic fall in sea-level. Evidence for this sea-level fall in Libya and Jordan is manifested in a wide variety of palynological proxy data (including reduced acritarch and chitinozoan total abundance, reduced acritarch diversity and changes in the relative abundances of various environmentally sensitive acritarch morphogroups) and from the presence of a minor positive δ13Corg excursion at the same stratigraphical level as the ‘hot’ shale. The environment of deposition was anoxic before, during and after deposition of the ‘hot’ shale, except for some very brief incursions of more oxygenated water that enabled the development of a very limited burrowing benthos and graptolite preservation as three-dimensional pyrite internal moulds. These incursions occurred just before and during deposition of the ‘hot’ shale and are interpreted as resulting from the influx of sediment-laden oxygenated currents from shallower water environments. Enhanced organic matter preservation within the ‘hot’ shale is attributed to greater productivity resulting from increased nutrient input, particularly from wind-blown dust, more rapid descent through the water column of organic matter in post-bloom marine snow macroaggregates, development of geochemically sealing microbial mats at the sea floor and more rapid burial of organic material than had occurred earlier in the Rhuddanian.

A comment on ‘Biochar and its effects on plant productivity and nutrient cycling: a meta‐analysis': on the importance of accurate reporting in supporting a fast‐moving research field with policy implications

A comment on ‘Biochar and its effects on plant productivity and nutrient cycling: a meta‐analysis': on the importance of accurate reporting in supporting a fast‐moving research field with policy implications

Changes in sub-fossil chironomid assemblages in two Northern Patagonian lake systems associated with the occurrence of historical fires

Patagonia is commonly seen as an exceptionally pristine area because of its wildlife and practically unpolluted waters. However, during the twentieth century the burning of natural forests was one of the most important human activities in Northern Chilean Patagonia. Some estimations indicate that three million hectares were burned during the first three decades of the century. Hence the objective of this study was to evaluate the impacts of the historical fires in Lake Burgos (45o42′S) and Lake Thompson (45o38′S) in Chilean Patagonia. The impact was measured by evaluating chironomid assemblage since they are sensitive enough to be used as an indicator of aquatic ecosystem health. Fires have a direct and drastic effect on a lake watershed but also indirectly affect a lake ecosystem, changing sedimentation patterns or increasing nutrient inputs. In the studied lakes the periods with higher prevalence of fires were identified by charcoal analysis, while organic matter and magnetic susceptibility allowed the confirmation of pre-fire and post-fire periods. The chironomid composition was evaluated through a PCA and an analysis of similarity (ANOSIM) to test the significance among periods while a Detrended Correspondence Analysis was applied to the chironomid assemblage downcore to assess compositional structure and taxa turnover. In Lake Burgos the ANOSIM test indicated significant differences between the pre-fire and fire periods (p < 0.05), while in Lake Thompson differences were not significant. However, in Lake Thompson the PCA clearly separated the pre-fire from the fire period but not the fire from the post-fire periods. In both lakes chironomid composition changed in relation to the period of higher prevalence of fires, which in turn implies catchment changes, pollution, and other anthropogenic impacts. Particularly a marked change in mesotrophic/eutrophic taxa was detected, reflecting an increase in nutrient input due to deforestation. Our findings point out that the lacustrine ecosystems are still affected by the impact of fires and the subsequent increase in nutrient supply that occurred almost 50 years ago. No sign of reverting to pre-disturbance conditions was observed, which makes these lakes highly sensitive to current human-induced impacts.

Establishing past environmental conditions and tracking long-term environmental change in the Canadian Maritime provinces using lake sediments

Freshwater lakes in the Canadian Maritime provinces have been detrimentally influenced by multiple, often synergistic, anthropogenically-sourced environmental stressors. These include surface-water acidification (and a subsequent decrease in calcium loading to lakes); increased nutrient inputs; watershed development; invasive species; and climate change. While detailed studies of these stressors are often hindered by a lack of predisturbance monitoring information; in many cases, these missing data can be determined using paleolimnological techniques, along with inferences on the full extent of environmental change (and natural variability), the timing of changes, and linkages to probable causes for change. As freshwater resources are important for fisheries, agriculture, municipal drinking water, and recreational activities, among others, understanding long-term ecological changes in response to anthropogenic stressors is critical. To assess the impacts of the major water-quality issues facing freshwater resources in this ecologically significant region, a large number of paleolimnological studies have recently been conducted in Nova Scotia and southern New Brunswick. These studies showed that several lakes in southwestern Nova Scotia, especially those in Kejimkujik National Park, have undergone surface-water acidification (mean decline of 0.5 pH units) in response to local-source SO2emissions and the long-range transport of airborne pollutants. There has been no measureable chemical or biological recovery since emission restrictions were enacted. Lakewater calcium (Ca) decline, a recently recognized environmental stressor that is inextricably linked to acidification, has negatively affected the keystone zooplankter Daphnia in at least two lakes in Nova Scotia (and likely more), with critical implications for aquatic food webs. A consistent pattern of increasing planktonic diatoms and scaled chrysophytes was observed in lakes across Nova Scotia and New Brunswick, suggesting that the strength and duration of lake thermal stratification has increased since pre-industrial times in response to warming temperatures (∼1.5 °C since 1870). These include three lakes near Bridgewater, Nova Scotia, that are among the last known habitat for critically endangered Atlantic whitefish (Coregonus huntsmani). Overall, these studies suggest that aquatic ecosystems in the Maritime Provinces are being affected by multiple anthropogenic stressors and paleolimnology can be effective for inferring the ecological implications of these stressors.

Phytoplankton of the Amur River Estuary (Sea of Okhotsk) during the summer periods of 2005–2007

The qualitative composition of planktonic microalgae in the Amur River Estuary (in the Sea of Okhotsk) was studied in July 2005, June 2006, and June 2007. The biomass and abundance of phytoplankton were studied for the first time. The average cell density of the microalgae ranged from 194700 to 855100 cells/liter, being higher in 2006 than in 2005 and 2007. The average biomass of the community varied from 1.06 g/m3 in 2007 to 3.17 g/m3 in 2006. Analysis of the similarity of the species composition and cell density of the phytoplankton revealed two groups of stations: the first includes stations in the northern and central parts of the Amur Estuary; and the second includes stations in the southern and central parts. The first group was characterized by the predominance of freshwater diatoms and a high abundances of green and blue-green algae and the second group was characterized by the predominance of marine microalgae. The high cell density and biomass of phytoplankton were due to the increased nutrient input via the river runoff of the Amur.

Factors controlling primary productivity in a wet–dry tropical river

Algal production in rivers fuels foodwebs, and factors controlling this production ultimately affect food availability. Conversely, excessive algal production can have negative effects on higher trophic levels. The present study examined permanent waterholes in a disconnected wet–dry tropical river to determine the controls on algal production. Primary production in this river system was high compared with arid-zone and perennially flowing tropical rivers. Phytoplankton biomass increased over the dry season but this appeared to be because waterhole volume decreased, due to evaporation. Nitrogen (N) was the key limiting nutrient for phytoplankton, with rapid N turnover times, depletion of particulate 15N-nitrogen reflecting increasing N fixationover the dry season, and N stimulation in phytoplankton bioassays. The waterholes were shallow, providing sufficient light for accumulation of benthic algal biomass. Exclosure experiments were also conducted to determine the impact of top–down control on benthic algal biomass, with no evidence that exclusion of fish and crustaceans increased benthic algal biomass. The shallow off-channel waterhole in our study had substantially higher concentrations of nutrients and chlorophyll a than did the on-channel waterholes. This suggests that future anthropogenic changes, such as increased water extraction and increased nutrient inputs, could make the waterholes more vulnerable to deteriorating water quality, such as e.g. algal blooms, low concentrations of dissolved oxygen.

Effects of nutrient loading on the trophic state of Lake Brunner

Lake Brunner, an oligotrophic monomictic lake on the West Coast of the South Island of New Zealand, is under pressure from urban expansion and increased farming activity, which has led to concern for the effects on water quality in the lake. Epilimnetic nitrogen, phosphorus and chlorophyll a concentrations have increased since 1992, and Secchi depth decreased. This suggests an increased algal productivity caused by increased nutrient inputs, further supported by increased hypolimnetic oxygen depletion since 1992. These observations are likely to have resulted from enhancement of pasture drainage and effluent inputs from expanding dairy farms. The Vollenweider model predicted a mean phosphorus concentration in the lake close to that observed, from estimated catchment loading, suggesting that the Vollenweider model adequately estimated the retention of phosphorous. With the Vollenweider model the effects of potential future loading scenarios were explored. Modelling suggested that a 70% increase in phosphorus loading could turn the lake into a mesotrophic state. Trend analysis of total phosphorus suggests that, with present land uses in the catchment (intensive dairy farming) continuing to develop at the same rate using the same land management practises, this transition to a mesotrophic state will occur by 2040.

Changes in the biomass of chambo in the southeast arm of Lake Malawi: A stock assessment of Oreochromis spp.

Lake Malawi has one of the most diverse fish faunas in the world (500–650 species) and is a major source of protein for the people of Malawi. Chambo (Oreochromis spp.) is one of the most important food fishes; its abundance has declined sharply over the last twenty-years. Surveys by the Malawi Department of Fisheries have shown a decrease in chambo density in the southeast arm of the lake and the annual harvest has dropped substantially since 1985. We conducted a dynamic stock assessment of Oreochromis spp. which included all vessel and gear types and covered the entire southeast arm of Lake Malawi. Chambo biomass peaked in 1982 and then declined continuously through the early 2000s. The biomass is highly correlated with the mean lake height two years prior suggesting that recruitment may be linked to increased nutrient input, and spawning and nursery habitat associated with the flooding of low lying areas. The main driver of chambo biomass, however, was fishing pressure which was above the level that would achieve maximum sustainable yield during the entire time series. This study provides a baseline from which to measure changes due to future management actions or climate variations.

Global Change and Response of Coastal Dune Plants to the Combined Effects of Increased Sand Accretion (Burial) and Nutrient Availability

Coastal dune plants are subjected to natural multiple stresses and vulnerable to global change. Some changes associated with global change could interact in their effects on vegetation. As vegetation plays a fundamental role in building and stabilizing dune systems, effective coastal habitat management requires a better understanding of the combined effects of such changes on plant populations. A manipulative experiment was conducted along a Mediterranean dune system to examine the individual and combined effects of increased sediment accretion (burial) and nitrogen enrichment associated with predicted global change on the performance of young clones of Sporobolus virginicus, a widespread dune stabilizing species. Increased burial severity resulted in the production of taller but thinner shoots, while nutrient enrichment stimulated rhizome production. Nutrient enrichment increased total plant biomass up to moderate burial levels (50% of plant height), but it had not effect at the highest burial level (100% of plant height). The effects of such factors on total biomass, shoot biomass and branching were influenced by spatial variation in natural factors at the scale of hundreds of metres. These results indicate that the effects of burial and nutrient enrichment on plant performance were not independent. Their combined effects may not be predicted by knowing the individual effects, at least under the study conditions. Under global change scenarios, increased nutrient input could alleviate nutrient stress in S. virginicus, enhancing clonal expansion and productivity, but this benefit could be offset by increased sand accretion levels equal or exceeding 100% of plant height. Depletion of stored reserves for emerging from sand could increase plant vulnerability to other stresses in the long-term. The results emphasize the need to incorporate statistical designs for detecting non-independent effects of multiple changes and adequate spatial replication in future works to anticipate the impact of global change on dune ecosystem functioning.

Tropical Andean forests are highly susceptible to nutrient inputs--rapid effects of experimental N and P addition to an Ecuadorian montane forest.

Tropical regions are facing increasing atmospheric inputs of nutrients, which will have unknown consequences for the structure and functioning of these systems. Here, we show that Neotropical montane rainforests respond rapidly to moderate additions of N (50 kg ha−1 yr−1) and P (10 kg ha−1 yr−1). Monitoring of nutrient fluxes demonstrated that the majority of added nutrients remained in the system, in either soil or vegetation. N and P additions led to not only an increase in foliar N and P concentrations, but also altered soil microbial biomass, standing fine root biomass, stem growth, and litterfall. The different effects suggest that trees are primarily limited by P, whereas some processes—notably aboveground productivity—are limited by both N and P. Highly variable and partly contrasting responses of different tree species suggest marked changes in species composition and diversity of these forests by nutrient inputs in the long term. The unexpectedly fast response of the ecosystem to moderate nutrient additions suggests high vulnerability of tropical montane forests to the expected increase in nutrient inputs.

Recent human-induced trophic change in the large and deep perialpine Lake Lucerne (Switzerland) compared to historical geochemical variations

This study investigates the sedimentological and geochemical changes that occurred during the last 2200years in the meromictic Lake Lucerne (Switzerland), one of the largest freshwater lakes of Central Europe. The stable isotope composition (δ13C and δ18O values) of bulk carbonates is compared to changes in grain-size distribution (clay and silt fraction), natural trace element input (titanium and thorium concentrations), and organic material abundance (Corg, nitrogen and phosphorus) and composition (C/N ratios and hydrogen and oxygen indexes). A drop in carbonate accumulation and in the δ18O values of sediments between ca. AD 500 and 700 followed a large and consistent rise in chemical weathering, marked by increases in the silicate-clay fraction and in crustal element concentrations. During the following millennium, there was a long-term decreasing trend in the lithogenic trace element input and in the phosphorus loading, suggesting decreasing terrigeneous input from runoff water. The major sedimentological change over the studied period occurred after ca. AD 1800 with a significant increase in the erosion-driven silt-fraction and in the sedimentation rate. During the last century, human-induced increase in nutrient input to the lake highly enhanced the accumulation of organic matter in sediment. Changes in nutrients and oxygen conditions in the hypolimnion of Lake Lucerne during the eutrophication period (i.e., the last 40years) highly modified the geochemical fluxes compared to the relatively stable oligotrophic conditions that prevailed during the previous 2000years. Before the 19th century, climate driven meromixis had a limited impact on the organic matter flux to the sediments, but the accumulation of carbonate considerably decreased during periods of lower mechanical erosion rates and high chemical weathering rates.

Controls on phytoplankton productivity in a wet–dry tropical estuary

Flood events typically enhance primary productivity in estuaries via the increased nutrient inputs from land runoff. This study examined the drivers of phytoplankton biomass accumulation and productivity in a tropical estuary with a distinct wet–dry seasonality, i.e. months of little or no rainfall, and a highly episodic extended wet season. The study found that over two wet seasons, there was little evidence of freshwater inputs increasing nutrient concentrations, and chlorophyll a concentrations and phytoplankton productivity rates decreased in the water column, probably due to low water residence times. The magnitude and duration of freshwater flows in the wet season appeared to affect the scale of reduction of phytoplankton productivity and biomass accumulation. In contrast to many studies, there was also no evidence of post-flooding stimulation of chlorophyll a concentration with net export of nutrients in both the wet and dry seasons. Nitrogen (N) and light appeared to be key limiting factors for phytoplankton growth with estuarine DIN rapidly turned over by phytoplankton, no evidence of N fixation by phytoplankton, and a response to N, but not phosphorus (P), in algal bioassays. Tidal resuspension of sediments was an important physical process that limited light availability for primary productivity. The lack of higher nutrient concentrations as a result of freshwater inputs, and lack of post-flood algal growth stimulation contrasts with the findings of studies in eutrophied systems.

Shallow water carbonate platforms (Late Aptian–Early Albian, Southern Apennines) in the context of supraregional to global changes: re-appraisal of palaeoecological events as reflectors of carbonate factory response

Abstract. This paper discusses the palaeoenvironmental significance of the "Orbitolina Level", the microbial carbonates and the Salpingoporella dinarica-rich deposits encased in the Aptian/Albian shallow water carbonate platform strata of Monte Tobenna and Monte Faito (Southern Italy). These facies show a peculiar field appearance due to their color and/or fossil content. In the shallow water carbonate strata, the Late Aptian "Orbitolina Level" was formed during a period of decreasing accommodation space. Microbial carbonates occur in different levels in the composite section. They reach their maximum thickness around the sequence boundaries just above the "Orbitolina Level" and close to the Aptian–Albian transition, and were not deposited during maximum flooding. S. dinarica-rich deposits occur in the lower part of the Monte Tobenna-Monte Faito composite section, in both restricted and more open lagoonal sediments. S. dinarica has its maximum abundance below the "Orbitolina Level" and disappears 11 m above this layer. On the basis of δ13C and δ18O values recorded at Tobenna-Faito, the succession has been correlated to global sea-level changes and to the main volcanic and climatic events during the Aptian. Deterioration of the inner lagoon environmental conditions was related to high trophic levels triggered by volcano-tectonic activity. Microbial carbonates were deposited especially in periods of third-order sea level lowering. In such a scenario, periods of increased precipitation during the Gargasian induced the mobilization of clay during flooding of the exposed platform due to high-frequency sea-level changes, with consequent terrigenous input to the lagoon. This and the high nutrient levels made the conditions unsuitable for the principle carbonate producers, and an opportunistic biota rich in orbitolinids (Mesorbitolina texana and M. parva) populated the platform. In the more open marine domain, the increased nutrient input enhanced the production of organic matter and locally led to the formation of black shales (e.g. the Niveau Fallot in the Vocontian Basin). It is argued that the concomitant low Mg/Ca molar ratio and high concentration of calcium in seawater could have favoured the development of the low-Mg calcite skeleton of the S. dinarica green algae. During third-order sea-level rise, no or minor microbial carbonates formed in the shallowlagoonal settings and S. dinarica disappeared. Carbonate neritic ecosystems were not influenced by the environmental changes inferred to have been induced by the mid-Cretaceous volcanism. The "Orbitolina Level", the microbial carbonates and the Salpingoporella dinarica-rich deposits in the studied Aptian/Albian shallow water carbonate strata are interpreted to be the response to environmental and oceanographic changes in shallow-water and deeper-marine ecosystems.

Nitrogen and phosphorus inputs to the Black Sea in 1970–2050

Increased nitrogen (N) and phosphorus (P) inputs are major causes of eutrophication in the coastal waters of the Black Sea. The objective of this study is to analyze the past and future trends in river export of nitrogen and phosphorus to the coastal waters of the Black Sea and to assess the associated potential for coastal eutrophication. The Global NEWS-2 (Nutrient Export from WaterSheds) model was used for this purpose. Currently, most eutrophication occurs in the North Black Sea and the Azov Sea. In the future, however, this may change. We analyzed trends up to 2050 on the basis of the Millennium Ecosystem Assessment (MEA) scenarios. The results indicate that nutrient loads in rivers draining into the North Black Sea and the Azov Sea may decrease in the coming decades as a result of agricultural trends and environmental policy. However, in these scenarios, the targets of the Black Sea Convention are not met. In the South Black Sea, there is currently little eutrophication. But this may change because of increases in nutrient inputs from sewage in the future.

Fifty years of change in Central European grassland vegetation: Large losses in species richness and animal-pollinated plants

There is growing concern that biodiversity loss in European agricultural landscapes is having negative effects on functional trait diversity. Long-term studies examining vegetation changes from the period before agricultural industrialisation are however rare. Here, we ask how management intensification and increased nutrient input initiated in the 1950/1960s have altered grassland plant community composition, species diversity and functional trait composition using comprehensive datasets from five floodplain regions (plus one protected reference region) in northern Germany. Sites with available historical relevés and vegetation maps (1950/1960s, 1990s) were resampled in 2008 to facilitate the analysis of a period spanning four to five decades.Plant community composition changed tremendously in all study regions during the 50year period, which was related to increasing Ellenberg indicator values for nutrient availability. Species richness at the plot-level fell by 30–50% over the period, and losses in functional diversity were equally large. A non-formal comparison with the results from the protected reference study region indicates that the changes may mostly be attributable to local nutrient input rather than to supra-regional climate change. Our results indicate a consistent trend towards much more species-poor communities dominated by mow-tolerant, N-demanding competitive grasses, whereas species with more ruderal strategies, species flowering early in the season and, in particular, insect-pollinated herbs have all decreased. The substantial loss of nectar-producing grassland herbs is likely to have negative effects on the abundance of pollinating insects, with consequences for the grassland animal communities. This highlights the growing need for adequate grassland management schemes with low N input to preserve high-nature-value grassland.

Determinants of fertiliser use by smallholder maize farmers in the Chinyanja Triangle in Malawi, Mozambique and Zambia

Farm surveys in Malawi, Zambia and Mozambique were carried out to assess the determinants of fertiliser use given continued low yields, low organic matter and general poor soil health in southern African soils. Regression modelling showed that fertiliser use was influenced by household and farm characteristics. In addition, it was also influenced by social and human capital and farmers’ perceptions of the effect of fertilisers on soil fertility. Farmers who perceived fertilisers as bad for their soil were less likely to adopt their use. This is a key result, as the emerging discussions on a green revolution for Africa, as well as the continued food crisis discussion, are prompting increased fertiliser use as an immediate intervention for increasing nutrient inputs into agriculture in the developing world. Increased policy efforts should be placed not only on increasing access to fertilisers but also on evolving farmers’ perceptions and attitudes towards fertiliser use.

Impacts of human activities on nutrient transports in the Huanghe (Yellow River) estuary

The impacts of anthropogenic activities on nutrient transport in the Huanghe/estuary were investigated using biogeochemical observations carried out during 2008–2009 to examine how nutrient change during a water–sediment regulation event. Nutrient concentrations in the Huanghe are characterized by relative high concentrations of nitrate and dissolved silicate but low phosphate and DOP levels and shows seasonal variations with very high DIN/PO43- ratios. The water–sediment regulation event resulted in high monthly average water discharge and sediment load occurring at least 2 months prior to before the event. The nutrient transport fluxes increased 8–30 times during that period. The regulation event has shifted the seasonal patterns of water and nutrient transport, which cannot only increase nutrient inputs to the coastal ecosystem but can also result in nutrient imbalance, affecting phytoplankton production and composition.