Autism Spectrum Disorder (ASD) is a growing social health issue. Primary healthcare institutions are where children most commonly seek help, and family physicians in primary care play a crucial role in early ASD detection. This study aims to assess the knowledge level, behaviours, and attitudes of family physicians and family medicine residents across Turkey, identify factors affecting their knowledge, and raise awareness among physicians. This descriptive cross-sectional study was conducted between March and June 2022, with the participation of family medicine specialists, residents, and practicing physicians in Turkey. A 50-question survey was administered face-to-face and digitally. The survey comprised 31 questions on sociodemographic information, factors influencing knowledge, attitudes and behaviours about ASD. The second part included a 19-question "Knowledge about childhood autism among health workers" (KCAHW) questionnaire. The study included 392 physicians with a mean age of 32.8 ± 7.9; 60.7% were women. Among the participants, 50.3% were family medicine residents, 21.7% were family medicine specialists, 17.0% were family physicians and 11.0% were contracted family medicine residents. The physicians' mean KCAHW score was 14.1 ± 2.6. Screening for ASD and following ASD were significantly associated with the perception of knowledge level (p = 0.004; p = 0.028, respectively). A statistically significant relationship was found between paediatric rotation, education about ASD, patient follow-ups and the KCAHW scores. Moreover, 98.2% of physicians believed children with ASD should receive special education, while 79.6% thought ASD carried a social stigma, and 52% believed an ASD diagnosis could lead to discrimination. The study found that participants lacked knowledge about comorbidities with ASD, atypical presentations of ASD, and guiding individuals with ASD. However, having a paediatric rotation, receiving ASD training and following up on children with ASD were associated with increased knowledge levels. Screening for and following up on children with ASD also boosted physicians' self-confidence.
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