PURPOSE: It has recently been speculated that protein supplementation may further augment the adaptations to prolonged endurance exercise training. We assessed the impact of protein supplementation during prolonged endurance exercise training on whole-body oxidative capacity (VO2max) and endurance exercise performance. METHODS: Sixty recreationally active males (age: 27±6 y; BMI: 23.8±2.6 kg·m-2, VO2max 47±6 mL·min-1·kg-1) were subjected to 12 weeks of triweekly endurance exercise training. After each session and each night prior to sleep, participants ingested either a protein supplement (PRO; 29 g casein protein) or an isocaloric carbohydrate placebo (PLA). Before and after the 12 weeks of training, VO2max and endurance exercise performance (~10-km time-trial) were assessed on a cycle ergometer. Muscular endurance (total workload achieved during 30 reciprocal isokinetic contractions) was assessed by isokinetic dynamometry and body composition by DXA. Dietary intake was assessed at baseline and during the intervention period. Repeated measures ANOVA was applied to assess whether training adaptations were different between groups. RESULTS: Protein intake increased in PRO (1.2±0.4 to 1.6±0.3 g·kg-1), but not in PLA (1.3±0.4 to 1.2±0.3 g·kg-1, time x treatment interaction, P<0.001). Endurance exercise training induced an 11±6% increase in VO2max (time effect, P<0.001), with no differences between groups (PRO: 48±6 to 53±7 mL·min-1·kg-1; PLA: 46±5 to 51±6 mL·min-1·kg-1; time x treatment interaction, P=0.50). Time to complete the time-trial was reduced by 14±7% (time effect, P<0.001), with no differences between groups (time x treatment interaction, P=0.15). Muscular endurance increased by 6±7% (time effect, P<0.001), with no differences between groups (time x treatment interaction, P=0.84). Whole body lean mass was unchanged over time (P=0.097). However, leg lean mass showed an increase following endurance exercise training (P<0.001), which tended to be greater in PRO (PRO: 0.5±0.7 kg; PLA: 0.2±0.6 kg; time x treatment interaction, P=0.073). CONCLUSION: Protein supplementation after exercise and before sleep does not further augment the gains in whole-body oxidative capacity and endurance exercise performance following prolonged endurance exercise training in healthy, young males.
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