Increase In Longevity Research Articles

Control of Orthodontic Tooth Movement by Nitric Oxide Releasing Nanoparticles in Sprague-Dawley Rats.

Orthodontic treatment commonly requires the need to prevent movement of some teeth while maximizing movement of other teeth. This study aimed to investigate the influence of locally injected nitric oxide (NO) releasing nanoparticles on orthodontic tooth movement in rats.Materials and Methods:Experimental tooth movement was achieved with nickel-titanium alloy springs ligated between the maxillary first molar and ipsilateral incisor. 2.2 mg/kg of silica nanoparticles containing S-nitrosothiol groups were injected into the mucosa just mesial to 1st molar teeth immediately prior to orthodontic appliance activation. NO release from nanoparticles was measured in vitro by chemiluminescence. Tooth movement was measured using polyvinyl siloxane impressions. Bones were analyzed by microcomputed tomography. Local tissue was assessed by histomorphometry.Results:Nanoparticles released a burst of NO within the first hours at approximately 10 ppb/mg particles that diminished by 10 × to approximately 1 ppb/mg particles over the next 1–4 days, and then diminished again by tenfold from day 4 to day 7, at which point it was no longer measurable. Molar but not incisor tooth movement was inhibited over 50% by injection of the NO releasing nanoparticles. Inhibition of molar tooth movement occurred only during active NO release from nanoparticles, which lasted for approximately 1 week. Molar tooth movement returned to control levels of tooth movement after end of NO release. Alveolar and long bones were not impacted by injection of the NO releasing nanoparticles, and serum cyclic guanosine monophosphate (cGMP) levels were not increased in animals that received the NO releasing nanoparticles. Root resorption was decreased and periodontal blood vessel numbers were increased in animals with appliances that were injected with the NO releasing nanoparticles as compared to animals with appliances that did not receive injections with the nanoparticles.Conclusion:Nitric oxide (NO) release from S-nitrosothiol containing nanoparticles inhibits movement of teeth adjacent to the site of nanoparticle injection for 1 week. Additional studies are needed to establish biologic mechanisms, optimize efficacy and increase longevity of this orthodontic anchorage effect.

Versatile role of sirtuins in metabolic disorders: From modulation of mitochondrial function to therapeutic interventions

Sirtuins (SIRT1-7) are distinct histone deacetylases (HDACs) whose activity is determined by cellular metabolic status andnicotinamide adenine dinucleotide (NAD+ ) levels. HDACs of class III are the members of the SIRT's protein family. SIRTs are the enzymes that modulate mitochondrial activity and energy metabolism. SIRTs have been linked to a number of clinical and physiological operations, such as energy responses to low-calorie availability, aging, stress resistance, inflammation, and apoptosis. Mammalian SIRT2 orthologs have been identified as SIRT1-7 that are found in several subcellular sections, including the cytoplasm (SIRT1, 2), mitochondrial matrix (SIRT3, 4, 5), and the core (SIRT1, 2, 6, 7). For their deacetylase or ADP-ribosyl transferase action, all SIRTs require NAD+ and are linked to cellular energy levels. Evolutionarily, SIRT1 is related to yeast's SIRT2 as well as received primaryattention in the circulatory system. An endogenous protein, SIRT1 is involved in the development of heart failure and plays a key role in cell death and survival. SIRT2 downregulation protects against ischemic-reperfusion damage. Increase in human longevity is caused by an increase in SIRT3 expression. Cardiomyocytes are also protected by SIRT3 from oxidative damage and aging, as well as suppressing cardiac hypertrophy. SIRT4 and SIRT5 perform their roles in the heart. SIRT6 has also been linked to a reduction in heart hypertrophy. SIRT7 is known to be involved in the regulation of stress responses and apoptosis in the heart.

Telemedicine in sleep-related breathing disorders and treatment with positive airway pressure devices. Learnings from SARS-CoV-2 pandemic times.

The healthcare system currently faces new challenges, which are to be addressed by finding efficient alternatives. Such factors as the growth of world population, the increase in longevity, and the fact that some diseases which used to be deadly diseases have turned into chronic pathologies, cause the number of people in need for continuous medical care to rise. This results in a healthcare system crisis, which searches for solutions as telemedicine to address the needs of patients and control excessive medical spending. Telemedicine means remote medical assistance delivered by means of technological resources, which streamline the provision of medical care, thus increasing patient’s access to healthcare and saving time and costs. As regards respiratory diseases, telemedicine is a tool that may provide for proper prevention, diagnosis, therapeutic education, monitoring of observance, and therapeutic efficacy, as well as for the early detection of exacerbations. Patients suffering from sleep-related respiratory disorders in need for positive airway pressure devices may be benefited by telemedicine to enhance positive pressure adherence and follow-up to treat their pathologies, thus providing for the delivery of remote care and follow-up, reducing costs, and increasing the chances of receiving attention from specialists in patients who live a long distance from such medical facilities. However, it is a challenging task to find a balance in the doctor-patient virtual relationship.

Role of miRNAs in Neurodegeneration: From Disease Cause to Tools of Biomarker Discovery and Therapeutics.

Neurodegenerative diseases originate from neuronal loss in the central nervous system (CNS). These debilitating diseases progress with age and have become common due to an increase in longevity. The National Institute of Environmental Health Science’s 2021 annual report suggests around 6.2 million Americans are living with Alzheimer’s disease, and there is a possibility that there will be 1.2 million Parkinson’s disease patients in the USA by 2030. There is no clear-cut universal mechanism for identifying neurodegenerative diseases, and therefore, they pose a challenge for neurobiology scientists. Genetic and environmental factors modulate these diseases leading to familial or sporadic forms. Prior studies have shown that miRNA levels are altered during the course of the disease, thereby suggesting that these noncoding RNAs may be the contributing factor in neurodegeneration. In this review, we highlight the role of miRNAs in the pathogenesis of neurodegenerative diseases. Through this review, we aim to achieve four main objectives: First, we highlight how dysregulation of miRNA biogenesis led to these diseases. Second, we highlight the computational or bioinformatics tools required to identify the putative molecular targets of miRNAs, leading to biological molecular pathways or mechanisms involved in these diseases. Third, we focus on the dysregulation of miRNAs and their target genes leading to several neurodegenerative diseases. In the final section, we highlight the use of miRNAs as potential diagnostic biomarkers in the early asymptomatic preclinical diagnosis of these age-dependent debilitating diseases. Additionally, we discuss the challenges and advances in the development of miRNA therapeutics for brain targeting. We list some of the innovative strategies employed to deliver miRNA into target cells and the relevance of these viral and non-viral carrier systems in RNA therapy for neurodegenerative diseases. In summary, this review highlights the relevance of studying brain-enriched miRNAs, the mechanisms underlying their regulation of target gene expression, their dysregulation leading to progressive neurodegeneration, and their potential for biomarker marker and therapeutic intervention. This review thereby highlights ways for the effective diagnosis and prevention of these neurodegenerative disorders in the near future.

Increased risk of cancer in dogs and humans: a consequence of recent extension of lifespan beyond evolutionarily-determined limitations?

Cancer is among the most common causes of death for dogs (and cats) and humans in the developed world, even though it is uncommon in wildlife and other domestic animals. We provide a rationale for this observation based on recent advances in our understanding of the evolutionary basis of cancer. Over the course of evolutionary time, species have acquired and fine-tuned adaptive cancer protective mechanisms that are intrinsically related to their energy demands, reproductive strategies, and expected lifespan. These cancer protective mechanisms are general across species and/or specific to each species and their niche, and they do not seem to be limited in diversity. The evolutionarily acquired cancer-free longevity that defines a species' life history can explain why the relative cancer risk, rate, and incidence are largely similar across most species in the animal kingdom despite differences in body size and life expectancy. The molecular, cellular, and metabolic events that promote malignant transformation and cancerous growth can overcome these adaptive, species-specific protective mechanisms in a small proportion of individuals, while independently, some individuals in the population might achieve exceptional longevity. In dogs and humans, recent dramatic alterations in healthcare and social structures have allowed increasing numbers of individuals in both species to far exceed their species-adapted longevities (by 2-4 times) without allowing the time necessary for compensatory natural selection. In other words, the cancer protective mechanisms that restrain risk at comparable levels to other species for their adapted lifespan are incapable of providing cancer protection over this recent, drastic and widespread increase in longevity.

Longevity of complete dentures after relines: A 20-year population based retrospective study of 187,227 publicly insured adults

To evaluate the incidence of the first complete denture reline in a population of publicly insured adults and to assess its association with complete denture longevity. The records of 187,227 Australian adults who accessed subsidized complete denture treatment through public clinics were assessed. The number of years from denture issue to denture replacement was modelled using Poisson regression. Over 20 years, 5.9% of participants received at least one reline. The incidence rate (IR) for relines was highest in the first year of denture life, (IR: 0.031, 95%CI 0.030 to 0.032) and decreased as dentures increased in age. Dentures receiving an early reline (within 12 months of denture issue) had a mean longevity of 5.03 years (SD: 3.76) and dentures which received a late reline had a mean longevity of 7.12 years (SD: 3.32). Multivariate Poisson regression found that dentures which received an early reline were associated with a 2% reduction in denture longevity in comparison to those who did not receive a reline (IRR: 0.98, 95%CI: 0.97 to 0.99, p<0.001). Dentures which received a late reline were associated with a 15% increase in longevity in comparison to dentures which received no reline (IRR: 1.15, 95%CI: 1.13 to 1.16, p<0.001). The incidence of relining in this population was low. The results show that the timing of a reline during a denture's life modulates its effect on denture longevity. Denture longevity was prolonged in those receiving a late reline and was reduced in those receiving an early reline. Relines performed after at least 12 months of denture issue can be expected to increase the longevity of complete dentures. This effect was not observed for relines performed within the first 12 months of denture provision.

Safety and Protective Activities of Manufactured Alcohol-Free Beers

Nowadays, a general interest in improving health in order to achieve better conditions of life is increasing. Diet is a complex factor affecting health conditions. We analysed the biological activities of three types of alcohol-free lager beer (a blond, a pale-blond and a stout beer) as well as epicatechin gallate (ECG) as one of their most abundant phenols with the aim of revealing them as nutraceuticals. For that purpose, we carried out safety and protective assays of the tested substances in the well-known Drosophila melanogaster animal model. Moreover, chemoprevention studies on human leukaemia cells (HL-60) in an in vitro model were carried out to evaluate the viability and genomic damage potential of the studied compounds on the tumour cell line. Results suggest the safety properties of all compounds, although pale-blond and stout beer only showed genotoxic activity at the lowest concentrations assayed. Moreover, alcohol-free beers and phenols were able to protect against H2O2 oxidative damage as well as to induce an increase in longevity with an improvement of the quality of life in the in vivo animal model assayed. Promising results were obtained with the alcohol-free beers and ECG in the in vitro assays with human leukaemia cells as they inhibited the tumour cells’ growth, induced DNA damage and modified the methylation status of such a cancer cell line. To sum up, alcohol-free beers should be of interest not only because of their reduced calories and isotonic properties but because they can be recognised as nutraceutical substances.

Normalized lifespan inequality: disentangling the longevity\u2013lifespan variability nexus

Previous studies have documented a historically strong and negative association between countries’ life expectancy (i.e., average longevity) and length-of-life inequality (i.e., variability in ages at death). The relationship between both variables might be partially explained by life expectancy increasing at a faster pace than maximal length of life, a phenomenon that mechanically compresses the age-at-death distribution and has not been taken into consideration in previous studies. In this paper, we propose a new approach to lifespan inequality measurement that accounts for the (uncertainly) bounded nature of length-of-life. Applying the new approach to the countries of the Human Mortality Database, we observe that the decline in overall lifespan variability typically associated with increases in longevity seems to stop and even reverse at higher levels of life expectancy. This suggests the emergence of worrying ethical dilemmas, whereby higher achievements in longevity would only be possible at the expense of higher lifespan variability.

Conserving Floral and Faunal Diversity of Rice Paddies

Rice paddies have complex trophic food webs of flora and fauna. Integrated biodiversity management of flowering forbs can help conserve the beneficial insects of rice ecosystem, in turn enhancing natural biological control of pests. A concept of habitat management through ecological engineering has been developed at the ICAR-Indian Institute of Rice Research, tested, and validated in farmers’ fields over several seasons of crop. The strategies are low cost, easy to adopt, ecological sound and environmentally friendly, while providing farmers with monetary returns and nutritional benefits. One of the techniques advocated, is increasing floral diversity. Floral diversity can be maintained by conserving commonly occurring flowering forbs in rice fields or by growing a crop plant that provides monetary and nutritional benefits. Flowering plants on rice bunds had significant impact on biodiversity of parasitoids such as Anagrus, Oligosita and others. Field studies through baiting and laboratory studies for longevity indicated enhanced parasitization rates and six to ten times increase in longevity of parasitoids. Significant differences were observed in parasitism of hopper eggs in plots with different crop borders in comparison to plots without flower borders (F= 4.91; df 7; p <0.01).

POSB296 Cost Efficiencies for Spinal Cord Stimulation in Denmark: Impact of Battery Longevity for Non-Rechargeable Devices and a 1-Stage Approach

Spinal cord stimulation (SCS) is a treatment for chronic intractable pain, with non-rechargeable (NR) devices a choice for patients who prefer a recharge-free option. Whilst SCS is a 2-stage procedure with a trial phase followed by permanent device implantation, a 1-stage approach can be used with equivalent safety and clinical outcomes. This analysis explores the costs of both approaches, incorporating battery longevity improvements amongst newer devices in Denmark (2021). Since 2019, three leading NR SCS devices have been launched: Device A (2019); Device B (2020); Device C (2021). Battery longevity of Devices A and B were obtained from product manuals. Longevity of the newest Device C was estimated at comparable stimulation settings for Device A (4.5 years vs. 8.31 for Device C) and Device B (5.1 years vs. 9.27 for Device C). A Markov model simulated individual patients across four scenarios: A vs. C and B vs. C with both a 2-stage and 1-stage approach. A cycle length of one month and a 15-year time horizon was used, with a Danish national payer perspective. Registry data informed withdrawal and adverse event probabilities. Unit costs were taken from national DRGs. Using a standard 2-stage SCS approach, undiscounted total per-patient costs for Device A vs. Device C were DKK 1,153,794 and DKK 863,493, respectively; for Devices B vs. Device C were DKK 1,039,492 and DKK 847,696, respectively. Employing a 1-stage approach saved DKK 237,420 in all scenarios. The cost savings driver was fewer expected device replacements afforded by increased battery longevity of the newest device. Increases in SCS battery longevity can translate into cost savings for healthcare payers, which can be extended by employing a 1-stage approach. Understanding the potential cost benefit of device choice and procedural optimisation may inform budgetary decisions for long-term cost management of SCS.

Marfatia Award Abstracts

With improving longevity and exponential increase of age related illnesses, MCI and dementia are projected to be among the major causes of suffering in old age. The containment of these problems rests with their early detection and prevention. While advanced Neuro imaging techniques detect anatomical and structural changes at a later stage, QEEG is considered an inexpensive, reliable method of detecting functional alterations in very early stages. Brain mapping with QEEG locates functional and electrical changes in the brain earlier than other methods and is reported to be useful in many clinical situations. QEEG reports are descriptive and 'pattern based' with an accuracy of 80-90 %. Specific patterns are associated with MCI and AD. The present study is a patternbased study done in a simple clinical setting, with a portable 4 channel analyser and dedicated software. The aim of the study was to know if QEEG can be used to predict / differentiate normal healthy aging from MCI/ dementia. Individuals in the age group of 30-72 with cognitive complaints underwent the test. The study confirms that QEEG is a valuable, inexpensive and reliable MARKER to predict / differentiate MCI, AD from healthy, normal aging.

Prevalence of Metabolic Syndrome and Specific Cardiovascular Risk Factors among Older Persons in the Philippines: Results from the 8th Philippine National Nutrition Survey.

Cardiovascular diseases are the leading causes of deaths among adults in the Philippines, and this is true also among older persons aged 60 years and above. Identification of risk factors and diseases that lead to cardiovascular mortality among the elderly is important to have an impact on longevity. This study aimed to determine the prevalence of metabolic syndrome and specific cardiovascular risk factors among older persons in the Philippines. A cross sectional design was used, with data taken from the results of the 8th Philippine National Nutrition Survey (NNS) Clinical and Health Survey done in 2013. Although there is a more recent survey, only the 2013 data is complete and available for secondary analyses. There were 1,835 older persons who were participants in the 8th Philippine NNS who had complete clinical data. The prevalence of Metabolic Syndrome was 52.6% in this population with the distribution of the components as follows: 33.5% have elevated waist circumference; 59.25 had elevated BP >130/85 mm Hg; 30.1% had fasting blood glucose ≥100 mg/dL (includes prediabetes and diabetes); 63% have low HDL, and 39% have elevated triglycerides ≥150 mg/dL.The results for other cardiovascular diseases and risk factors are as follows: 44% had hypertension using the criterion of the JNC VII report (BP ≥140/90 mm Hg); 85% had LDL cholesterol ≥100 mg/dL; 31% are current alcohol drinkers; 22% are current smokers; 53.7% have low physical activity; and 82% have an unhealthy diet. Fifty-two percent (52%) of older Filipinos have metabolic syndrome and have a high prevalence of cardio-vascular risk factors, foremost of which are elevated LDL-cholesterol at 85%, hypertension at 44% based on JNC VII, approximately 10% with diabetes mellitus, but with a double burden of overweight/obesity and undernutrition. This data can help plan for public health approaches to improve quality of life and increase longevity of Filipinos.

Down syndrome and Alzheimer’s disease: Same infectious cause, same preventive?

Advances in medicine and a corresponding increase in longevity have revealed an unexpected and startling phenomenon: as they age, individuals with Down syndrome are at an increased risk for developing Alzheimer’s disease. It also tends to appear in them earlier than is typically seen in classic cases. There is also rising evidence to suggest that the root cause of Alzheimer’s disease is infection from viral, bacterial, or parasitic microorganisms (e.g. Herpes Simplex Virus, Toxoplasma gondii, and more). The cause of Down syndrome is widely accepted to be “Trisomy 21,” but the root cause of that is unknown. I hypothesize that Down syndrome itself is caused by an Alzheimer’s-capable microbe, which in certain susceptible individuals is followed by Alzheimer’s disease, and the two illnesses proceed together. Understanding the relationship between Down syndrome, Alzheimer’s disease, and infections could be a critical step towards the prevention of both diseases.

PHYSICAL ACTIVITY AND CARDIOVASCULAR AGING: THE MECHANISM AND RECENT EVIDENCES

ABSTRACT&#x0D; Successful aging in population means preserving life quality and reducing the health burden. Many factors influence the successful aging, including physiological, psychological, social, and lifestyle factors. Physical activity is an important lifestyle factor that can prevent the chronic diseases, increase longevity and survival, and also improve cognitive and physical functions in elderly. The aim of this narrative review is to explain the correlation between physical activity and successful aging. Recent systematic review showed the benefit of exercise for overall health and cardiovascular system. Physical activity promotes successful aging among middle-aged and older adults especially in the younger population.&#x0D; &#x0D; Keywords : exercise; healthy life style; cardic aging

MENOPAUSE AS A FACTOR OF ORAL HEALTH

General increase of longevity in Ukraine and around the world along with the increasing number of women over 50 necessitates implementation of comprehensive scientifically based programs aimed to improve health and quality of life of this part of population.&#x0D; The aim of this review of international and Ukrainian studies was to investigate in depth hormone associated mechanisms of pathologic conditions formation in peri- and postmenopausal women.&#x0D; Materials and methods. Studies on association of climacteric symptoms and symptoms of dental pathology were analyzed.&#x0D; Results. Our literature review showed worse condition of dental health in menopausal women. The pres­ence of estrogen receptors in oral mucosa and salivary glands increases the risk of dental pathology when ovarian function diminishes. Gradual decrease of ovarian steroids in female body causes changes in oral microbiota and changes microbial landscape of periodontal pockets and effects local immunity. This causes increase of oral mucosa pathology such as glossodynia or burning mouth syndrome, lichen planus and angular cheilitis. It also causes the increase of periodontal diseases rate.&#x0D; Conclusions. Cross-disciplinary interaction of dentists and gynecologists-endocrinologists will provide possibilities to discover more common causes and pathogenic mechanisms of periodontal diseases and oral mucosa in menopausal women. This will provide possibilities for the development of comprehensive approach to diagnosis, treatment and prophylaxis.

MEF2 is a key regulator of cognitive potential and confers resilience to neurodegeneration.

Recent increases in human longevity have been accompanied by a rise in the incidence of dementia, highlighting the need to preserve cognitive function in an aging population. A small percentage of individuals with pathological hallmarks of neurodegenerative disease are able to maintain normal cognition. Although the molecular mechanisms that govern this neuroprotection remain unknown, individuals that exhibit cognitive resilience (CgR) represent a unique source of therapeutic insight. For both humans and animal models, living in an enriched, cognitively stimulating environment is the most effective known inducer of CgR. To understand potential drivers of this phenomenon, we began by profiling the molecular changes that arise from environmental enrichment in mice, which led to the identification of MEF2 transcription factors (TFs). We next turned to repositories of human clinical and brain transcriptomic data, where we found that the MEF2 transcriptional network was overrepresented among genes that are most predictive of end-stage cognition. Through single-nucleus RNA sequencing of cortical tissue from resilient and nonresilient individuals, we further confirmed up-regulation of MEF2C in resilient individuals to a subpopulation of excitatory neurons. Last, to determine the causal impact of MEF2 on cognition in the context of neurodegeneration, we overexpressed Mef2a/c in the PS19 mouse model of tauopathy and found that this was sufficient to improve cognitive flexibility and reduce hyperexcitability. Overall, our findings reveal a previously unappreciated role for MEF2 TFs in promoting CgR, highlighting their potential as biomarkers or therapeutic targets for neurodegeneration and healthy aging.

Effects of make-up on mood and walking in elderly women

The increase in longevity makes it necessary to take care of the quality of life of senior citizens. Ageing is accompanied by alterations leading to an increased risk of falling. A study conducted at the Charité Hospital in Saint-Etienne(42) showed that a cosmetic procedure with an impact on the appearance could improve the mood and the walking capacity of the senior citizen, but also have an effect on stress and immunity.

Invitro evaluation of silver nanoparticle of asparagus racemosus for it’s anti diabetic activity

Asparagus racemosus is an important medicinal plant that is found in tropical and subtropical zones in India belonging to the family Lilliacea. Its medicinal usage has been reported in the Indian and British Pharmacopoeias and in traditional systems of medicine such as Ayurveda, Unani, and Siddha. The dried root extract is used as a drug. Asparagus racemosus has been described to use as an antioxidant, antidyspepsia, antitussive, immune stimulant. Also used in the treatment of kidney disorder, chronic fever, liver cancer, stomach ulcer, increase milk secretion, and epilepsy. The major active constituents of Asparagus racemosus are steroidal saponins, isoflavones, polysaccharides, racemose, asparagamine, vitamins, mucilage, and folic acid. It is a commonly known Ayurvedic preparation useful to prevent aging, increase longevity, impart immunity and improve mental functions.

Effects of High-Sucrose and High-Saturated Fat Diets on Learning Abilities in Old Sprague-Dawley Rats

With an increase in longevity, many studies have explored the influences of different lifestyle factors on successful aging. This study hypothesizes that older rats fed with a nutritionally balanced standard diet would perform better on learning tasks than rats fed with either a high-sucrose or a high-saturated fat. It also hypothesizes that older rats fed with a high-sucrose diet would perform better than those fed with a high-saturated fat. The learning abilities of the 15-month-old rats (N = 36) were assessed by conducting forward and reverse learning tasks using a T-maze apparatus. The results showed that rats on a nutritionally balanced diet performed significantly better on both learning tasks than those on either the high-sucrose or the high-saturated fat (p’s &lt; .05). This may be due to the fact that the high-sucrose and high-saturated fat diets exacerbated a cognitive decline in geriatric rats. There was no significant difference between the learning abilities of the rats on a high-sucrose or high-saturated fat diet (p’s &gt; .05). This finding suggests that, at an older age, both high-sucrose and high-saturated fat diets have a similarly detrimental influence on cognitive health.

Extension of the vase life of Lilium pumilum cut flowers by pulsing solution containing sucrose, citric acid and silver thiosulfate

Abstract Floriculture is a promising activity and has great economic importance. In this context, the cultivation of Lilium pumilum Redouté stands out. However, one of the main challenges flower farmers face is that a large part of the products is lost before reaching the final consumer, due to postharvest losses. Such losses can be minimized by adopting some techniques, such as the use of conditioning solution (pulsing), which has been effective in preventing early senescence. In this sense, this study aimed to evaluate the effect of pulsing with sucrose, citric acid and silver thiosulphate on postharvest conservation of L. pumilum cut flowers. All treatments promoted a 1.8±0.3 day increase in longevity, uniformity of floral opening, and reduced fresh weight loss compared to the control (flowers that were not pulse-treated). There was no significant difference in chlorophyll content and leaf relative water content. The time of exposure to pulsing caused significant differences in the reduction of anthocyanin contents when compared to the control, demonstrating that pulsing preservative solution for at least 6 h extends the vase life of L. pumilum cut flowers by two days.