Increase In Lean Mass Research Articles

A probiotic mix partially protects against castration-induced bone loss in male mice.

Studies in postmenopausal women and ovariectomized mice show that the probiotic mix Lacticaseibacillus paracasei DSM13434, Lactiplantibacillus plantarum DSM 15312 and DSM 15313 (L. Mix) can protect from bone loss caused by sex steroid deficiency. Whether probiotic bacteria can protect bone also in sex steroid-deficient males is less studied. We used the orchiectomized mouse as a model for age-dependent bone loss caused by decreasing sex hormone levels in males. We treated 10-week-old male mice with either vehicle (veh) or L. Mix for 6 weeks, starting 2 weeks before orchiectomy (orx) or sham surgery. Importantly, mice treated with L. Mix had a general increase in total body bone mineral density (BMD) and lean mass (P ≤ 0.05) compared with veh-treated mice. Detailed computer tomography analysis of dissected bones showed increased trabecular BMD of the distal metaphyseal region of the femur in L. Mix compared to veh-treated orx mice (+8.0%, P ≤ 0.05). In the vertebra, L. Mix treatment increased trabecular bone volume fraction BV/TV (+11.5%, P ≤ 0.05) compared to veh in orx mice. Also, L. Mix increased the levels of short-chain fatty acids (SCFAs) such as propionate and acetate and important intermediates in SCFA synthesis such as succinate and lactate in the cecal content of male mice. In conclusion, L. Mix treatment resulted in a general increase in BMD in adult male mice and prevented trabecular bone loss in femur and vertebra of orx mice. These bone protective effects of L. Mix were associated with increased levels of SCFAs in the cecal content of male mice.

Gut microbiome: Linking together obesity, bariatric surgery and associated clinical outcomes under a single focus.

Obesity is increasingly prevalent in the post-industrial era, with increased mortality rates. The gut microbiota has a central role in immunological, nutritional and metabolism mediated functions, and due to its multiplexity, it is considered an independent organ. Modern high-throughput sequencing techniques have allowed phylogenetic exploration and quantitative analyses of gut microbiome and improved our current understanding of the gut microbiota in health and disease. Its role in obesity and its changes following bariatric surgery have been highlighted in several studies. According to current literature, obesity is linked to a particular microbiota profile that grants the host an augmented potential for calorie release, while limited diversity of gut microbiome has also been observed. Moreover, bariatric surgery procedures represent effective interventions for sustained weight loss and restore a healthier microbiota, contributing to the observed fat mass reduction and lean mass increase. However, newer evidence has shown that gut microbiota is only partially recovered following bariatric surgery. Moreover, several targets including FGF15/19 (a gut-derived peptide), could be responsible for the favorable metabolic changes of bariatric surgery. More randomized controlled trials and larger prospective studies that include well-defined cohorts are required to better identify associations between gut microbiota, obesity, and bariatric surgery.

High‐Fat Diet Exacerbates Androgen‐Mediated Obesity and White Adipose Tissue Hypertrophy in a Mouse Model of Polycystic Ovary Syndrome

BackgroundPolycystic Ovary Syndrome (PCOS) is the most common endocrine disorder in reproductive age women. PCOS is characterized by hyperandrogenemia, oligo‐ or anovulation and polycystic ovaries and is associated with obesity and white adipose tissue (WAT) dysregulation. The deleterious metabolic profile of women with PCOS is exacerbated by obesity. However, the molecular mechanisms that mediate WAT dysfunction in PCOS, as well as its modulation by the diet, are poorly understood. We aim to investigate the effect of an obesogenic diet on androgen‐mediated WAT hypertrophy by assessing the adipocyte size of the different WAT depots [mesenteric fat (MF), subcutaneous fat (SCF) and retroperitoneal fat (RPF)] using a well‐established model of PCOS in mice.MethodsThree‐week old female mice (C57BL/6J) were implanted with Silastic tubes filled with the androgen dihydrotestosterone (DHT, 8.0 mg, S.C.) or vehicle (empty tubes). Animals were fed high‐fat diet (HFD; 60% kcal fat) or low‐fat diet (LFD; 10% kcal fat, sucrose‐matched) for 90 days. Weekly body weights and body composition (EchoMRI) were assessed. Animals were sacrificed and multiple WAT depots (MF, SCF and RPF) were harvested for histological analysis. Formalin‐fixed paraffin‐embedded tissues from the three WAT depots were sectioned (5 µm thick) and stained with hematoxylin and eosin. The stained sections were imaged at 40X magnification. Adipocyte size (cross‐sectional area) was determined using ImageJ software with the Adiposoft plugin [~2,000 adipocytes/section, 3 sections/animal, n = 6/group]. GraphPad Prism 8.4.2 was used for statistical analysis, and P‐values of 0.05 were considered statistically significant.ResultsLFD‐fed DHT‐treated mice showed significant increases in body weight (28.00 ± 0.60 vs. 23.59 ± 0.41 g, p<0.05), lean mass (21.70 ± 0.30 vs. 20.04 ± 0.23 g, p<0.05), and fat mass (6.20 ± 0.50 vs. 3.42 ± 0.33 g, p<0.05) compared to their vehicle‐treated controls. Adipocyte size analysis showed that LFD‐fed DHT‐ treated mice have significantly larger adipocytes when assessed in MF (1512.0 ± 15.55 vs. 833.3 ± 6.50 µm2, p<0.05), SCF (1907.0 ± 22.04 vs. 1395.0 ± 12.43 µm2, p<0.05) and RPF (2284.0 ± 26.20 vs. 1396.0 ± 11.61 µm2, p<0.05), compared to their vehicle‐treated controls. Notably, HFD‐fed DHT‐treated mice showed greater MF and RPF adipocyte size compared to HFD‐fed vehicle‐treated mice, which was not observed in SCF. Interestingly, HFD exacerbated DHT‐mediated increases in body weight (1.54‐fold), lean mass (1.17‐fold), and fat mass (3.03‐fold) compared to LFD‐fed DHT‐treated mice. Additionally, the increase in adipocyte size in all fat depots in response to DHT was greater in mice fed HFD by 1.50‐fold compared to those fed LFD.Conclusion and significanceThe hypertrophic effect of androgens on the WAT is modulated by the diet. Increased adipocyte size is associated with insulin resistance in women with PCOS. Our results may help explain why obesity is associated with a worsened metabolic profile in women with PCOS at the cellular level. Weight loss with a low‐fat diet may be an effective approach to ameliorate WAT dysfunction and the subsequent metabolic dysregulation in PCOS.

Improving skeletal muscle insulin sensitivity via beta2 -agonist administration: a promising strategy to counteract metabolic disease and muscle loss.

Improving skeletal muscle insulin sensitivity via beta2 -agonist administration: a promising strategy to counteract metabolic disease and muscle loss.

49. LOSS OF HYPOXIA SIGNALING LIMITS PHYSIOLOGIC AND MUSCLE ADAPTATIONS TO AEROBIC EXERCISE IN AGING

PURPOSE: This study investigates signaling pathways which contribute to aging associated loss of muscle adaptation to exercise. METHODS: Young (Y, 12 weeks) and Old (O, 25 months) mice were subjected to regimented treadmill running or no exercise for 8 weeks. Mice were then subjected to muscle physiology testing. The transcriptional response to exercise was evaluated using RNA Seq. A genetically modified mouse model, with a deletion of aryl hydrocarbon receptor nuclear translocator (ARNT) was then evaluated for response to exercise training as it mimics old mice with regards to transcription profile and exercise response. RESULTS: Y, trained mice experienced a significant increase in distance running, speed, and lean muscle mass in comparison to untrained controls. O mice did not improve significantly in these measures. Transcriptome analysis in muscle from Y mice demonstrated differential regulation of 96 genes with exercise. None of these genes were similarly regulated in the O group. Genes most upregulated in Y mice are targets of hypoxia signaling. ARNT, a regulator of hypoxia signaling, increased 3-fold with exercise in Y mice, but not in O mice following exercise. To assess whether loss of ARNT impairs the exercise response, we generated a mouse with muscle-specific knockout of ARNT (ARNT muscle (mKO). Following regimented exercise, ARNT mKO mice did not improve maximal distance running, maximal running speed, or lean muscle mass. Administration of an ARNT agonist restored the exercise response. CONCLUSION: Restoration of ARNT and hypoxia signaling may restore the physiologic response to exercise in aging.

One Year of Recombinant Human Growth Hormone Treatment in Adults with Prader-Willi Syndrome Improves Body Composition, Motor Skills and Brain Functional Activity in the Cerebellum.

We compared body composition, biochemical parameters, motor function, and brain neural activation in 27 adults with Prader–Willi syndrome and growth-hormone deficiency versus age-and sex-matched controls and baseline versus posttreatment values of these parameters after one year of recombinant human growth hormone (rhGH) treatment. To study body composition, we analyzed percentage of fat mass, percentage of lean mass, and muscle-mass surrogate variables from dual X-ray absorptiometry. Biochemical parameters analyzed included IGF-I, glucose metabolism, and myokines (myostatin, irisin, and IL6). To explore muscle function, we used dynamometer-measured handgrip strength, the Timed Up and Go (TUG) test, and the Berg Balance Scale (BBS). To study brain activation, we acquired functional magnetic resonance images during three motor tasks of varying complexity. After one year of treatment, we observed an increase in lean mass and its surrogates, a decrease in fat mass, improvements in TUG test and BBS scores, and increased neural activation in certain cerebellar areas. The treatment did not significantly worsen glucose metabolism, and no side-effects were reported. Our findings support the benefits of rhGH treatment in adults with Prader–Willi syndrome and growth-hormone deficiency on body composition and suggest that it may also improve balance and brain neural activation.

Abstract EP46: Can Middle-Aged Recreational Cyclists Remain In Energy Balance And Weight Stable When Involved In An Extreme Exercise And Energy Expenditure Intervention (4E Study)? Effect On Visceral Adiposity And Features Of Cardiometabolic Risk

Introduction: Weight loss is often considered a key target in lifestyle interventions aimed at reducing cardiometabolic risk in people with overweight/obesity. However, evidence suggests that regular endurance exercise can induce a mobilization of visceral fat in the absence of weight loss. Therefore, the objective of the present study was to test the effect of an extreme exercise prescription not aimed at weight loss on visceral adiposity and on indices of cardiometabolic health. Hypothesis: Despite our attempt to keep the cyclists weight stable during the 1-week exercise intervention, a loss of visceral fat would be observed. Methods: Eleven male recreational cyclists, aged 50 to 66 years and asymptomatic for cardiovascular disease, cycled 1144 km (715 miles) over a period of seven consecutive days. During the intervention, body weight and composition were assessed daily by bioelectrical impedance and cyclists’ caloric intake was increased to keep them in energy balance and weight stable. Their cardiometabolic health profile was documented before and after the intervention and included a fasting blood test, a resting hemodynamic profile, anthropometric, body composition and body fat distribution measurements including visceral adiposity assessed by magnetic resonance. A maximal cardiopulmonary exercise test was performed at baseline. During the intervention, energy expenditure was calculated from the caloric equivalents of oxygen consumption for each heart rate value acquired with a heart rate monitor. Prepared foods were provided to participants and their energy intake was closely monitored. Results: Small decreases in body weight (-0.8 kg, P <0.05), BMI (-0.3 kg/m 2 , P <0.05) and fat mass (-1.5 kg, P =0.0005) as well as a trend towards an increase in lean mass (+0.8 kg, P =0.07) were observed. Visceral adiposity (-14.1 mL, P <0.01) and waist circumference (-3.2 cm, P <0.0001) decreased while subcutaneous adiposity remained unchanged (-2.7 mL, NS). Decreases in total cholesterol (-1.17 mmol/L, P <0.001), LDL cholesterol (-0.97 mmol/L, P <0.0001), non-HDL cholesterol (-1.32 mmol/L, P <0.0001), triglycerides (-0.77 mmol/L, P <0.005), glycated hemoglobin (-0.1%, P <0.005), and resting systolic (-9 mmHg, P <0.01) and diastolic (-7 mmHg, P <0.01) blood pressure were noted. Conclusion: This study showed that recreational master cyclists were not able to remain in energy balance when exposed to a very large exercise prescription performed over one week. However, despite trivial changes in body weight and no change in subcutaneous adiposity, a significant reduction in visceral adiposity was observed. This study shows the differential response of subcutaneous vs visceral adiposity to a very large prescription of endurance exercise, and the inability of body weight changes to fully appreciate the effects of endurance exercise on features of cardiometabolic risk.

Bicycle Touring 480 km in Seven Days: Effects on Body Composition and Physical Fitness-A Case Study.

Bicycle touring as a modality of physical activity that involves whole-body cardiorespiratory and metabolic functions could be a potential strategy to improve body composition and cardiorespiratory fitness. Therefore, the aim of the current study was to investigate the effects of 7-days bicycle touring activity on body composition and physical fitness. A total of 13 individuals (three women) participated in this quasi-experimental study. The participants were evaluated at baseline and were tracked for up to 12 days after the intervention. The intervention consisted of a 480 km bicycle touring route performed over 8 days. Body weight and body composition (i.e., fat mass and lean mass) were assessed using a bioelectrical impedance analyser. Physical fitness was measured using the International Fitness Scale questionnaire. We conducted a repeated-measures analysis of variance to determine changes in body weight and body composition and paired sample t-tests to analyse changes in physical fitness. Significant differences in fat mass were observed between the baseline and both post-intervention (p = 0.003) and re-test values (p = 0.031). Significant differences were also noted in lean mass between the baseline and post-intervention values (p = 0.003), whereas no significant changes were observed when compared the baseline and re-test values (p = 0.178). Significant differences were obtained in cardiorespiratory fitness when comparing the baseline with the post-intervention values (p = 0.016), whereas no significant differences were noted in general fitness, muscular strength, speed/agility, and flexibility (all p > 0.05). In conclusion, a 7-day bicycle touring intervention can significantly reduce fat mass and increase lean mass and cardiorespiratory fitness in healthy individuals.

The Effect of Different Periodization and Modes of Concurrent Strength and Endurance Training on Double Poling Performance and Body Composition in Adolescent Cross-Country Skiers.

The aim of the study was to compare the effects of different types and periodization of strength training on body composition and maximal aerobic performance in 10-week training period in adolescent XC skiers. Twenty-eight adolescent competitive cross-country skiers, including 10 females (age 17.9 ± 1.8 years; body mass 69.6 ± 9.7 kg; height 1.77 ± 0.1 m; training experience 8.6 ± 3.2 years) took part in this study. Pre-and post-intervention performance was measured with the incremental exercise test (Pmax) on a double poling ski ergometer. Changes in body composition were measured with DXA. In addition to regular endurance training, experimental group one (EXP1) performed maximal and explosive strength training two times per week, experimental group two (EXP2) performed maximal and explosive strength training 1–3 times per week, and the traditional (TRAD) group performed low intensity–high volume strength training 2 times per week. Increases in arm, trunk, and overall lean mass were found in TRAD (p < 0.05). Increases in arm lean-mass was found in EXP1 (p < 0.05), while no changes in body composition occurred in EXP2 (p ≥ 0.05). Pmax improved significantly in all groups (p < 0.05). Changes in body mass, overall and arm lean mass was related to changes in absolute performance (W; p < 0.05), while no relationships were found between changes in body composition parameters and relative performance (W/kg; p ≥ 0.05). In conclusion, different periodization of strength training led to similar improvements in double poling ergometer performance, but resulted in different changes in body composition (lean mass) in adolescent cross-country skiers.

Energy balance in hypothalamic obesity in response to treatment with a once-weekly GLP-1 receptor agonist.

Background/Objectives:Hypothalamic obesity (HO) frequently occurs following suprasellar tumors from a combination of decreased energy expenditure and increased energy intake. Glucagon-like peptide-1 receptor agonist (GLP1RA) therapy is associated with increased satiety and energy expenditure. We hypothesized GLP1RA therapy in patients with HO would cause both lower energy intake and increased energy expenditure.Subjects/Methods:Forty-two patients aged 10–26 years (median 16 years) with HO with suprasellar tumors were randomized to GLP1RA (exenatide extended release once-weekly, ExQW, n=23) or placebo (n=19). Thirty seven (81%) patients completed the 36-week double-blind placebo-controlled trial. Total energy expenditure (TEE) was measured with doubly labeled water, physical activity was assessed with actigraphy, and intake was estimated with ad libitum buffet meal. Results are presented as adjusted mean between-group difference.Results:As compared with treatment with placebo, treatment with ExQW was associated with decreased energy intake during a buffet meal (−1 800 kJ (−430 kcal), 95% CI −3 184 to −418 kJ, p= 0.02). There were no significant differences in physical activity between groups. ExQW (vs. placebo) treatment was associated with a decrease in TEE (−695 kJ/day (−166 kcal/day), 95% CI −1 130 to −264 kJ/day, p< 0.01, adjusted for baseline TEE). The treatment effect was still significant after further adjustment for change in body composition (−372 kJ/day (−89 kcal/day), 95% CI −699 to −42 kJ/day, p= 0.04) or change in leptin (−695 kJ/day (−166 kcal/day), 95% CI −1 130 to −264 kJ/day, p< 0.01). This decrease in TEE occurred despite an increase in lean mass and fat mass (1.7 vs. 1.3 kg lean mass, p= 0.88 and 1.5 vs. 4.6 kg fat mass, p= 0.04, ExQW vs. placebo).Conclusions:Treatment with a GLP1RA was associated with a decrease in food intake but also a decrease in TEE that was disproportionate to change in body composition.

Effect of milk protein concentrate supplementation on body composition and biochemical markers during a resistance training program

This study aimed to formulate a milk protein concentrate (MPC) and evaluate the effect of this MPC supplementation on body composition, lipid profile, and product acceptability in resistance training (RT) practitioners. The physical, chemical and sensory properties were then analyzed. To evaluate the effect of the MPC supplementation on resistance training practitioners, 30 males were divided into two groups: a control group and an intervention group. Then the participants in the intervention group were supplemented with 500 mL of the MPC (250 mL pre-RT and 250 mL post-RT). Body composition (weight, height, and skinfolds) and biochemical markers (cholesterol and triglycerides) were measured at baseline and at 30, 60, and 90 days after treatment. Body composition results showed that the MPC supplementation during a RT program significantly (p < 0.05) reduced body fat percentage and fat mass, and increased lean mass in RT practitioners. Characterization of the MPC revealed low fat (3.32 ± 0.08%) and carbohydrates (4.58 ± 0.59%), and high protein (18.51 ± 0.51%) concentrations. Additionally, the product showed satisfactory acceptability index (> 71%). Thus, the MPC supplementation contributed to reduction of fat percentage, fat mass, and increase of lean mass in RT practitioners, without lipid profile alterations.

Recomposição Corporal: seria possível induzir emagrecimento e hipertrofia muscular ao mesmo tempo?

abstract Adipose tissue reduction and lean mass increase are frequent goals in exercise programs aimed at health and aesthetics. In this context, when postulating the need for an energy deficit for weight loss and a caloric surplus for muscle hypertrophy, was developed the idea that it would not be possible for both phenomena to exist simultaneously. Contrarily, the term “Body Recomposition” (BR) emerges in the literature, a phenomenon in which weight loss and muscle hypertrophy occur at the same time. BR has already been observed using different techniques for analyzing body composition, from doubly indirect methods to magnetic resonance imaging, and in different population groups, namely: adolescents, sedentary or physically active adults, the elderly and people with excess weight, as well as practitioners of sports, including bodybuilding. BR occurs with precise nutritional adjustment, with protein consumption above the recommended daily intake (0.8 g/kg), in ranges between 2.4 and 3.4 g/kg of body mass/day. Different types of exercises can lead to BR, from strength training, through high-intensity circuit training, high-intensity interval training, and even concurrent training – most often with a high weekly frequency.

A hipertrofia muscular e o consumo de álcool

Muscle hypertrophy provides the improvement of physical aptitudes, health conditions, physical conditioning and longevity. Furthermore, alcohol consumption is a common practice in society, becoming an obstacle when individuals seek weight loss, improvement of physical or aesthetic condition. This is because alcohol consumption impairs muscle gain, as its intake breaks the balance, thus affecting homeostasis. In this context, the work aimed at alcohol consumption and vulnerabilities to hypertrophy in non-athletes. The study is a theoretical reflection of a qualitative approach, on the effects of the consumption of alcoholic beverages in the process of hypertrophy of the skeletal muscles of the human body. However, changes in habits favor the increase of lean mass, and many people who have their goals restrained by the use of alcohol seek in anabolic steroids the income that was lost in diet, metabolism or in the indisposition to practice caused by excess alcohol. Finally, moderate alcohol consumption is not an impediment to hypertrophy in non-athletes, and its use is highly prevalent in physically active individuals.

Lean Mass Improvement from Nutrition Education and Protein Supplementation among Rural Indian Women Living with HIV/AIDS: Results from Cluster Randomized Factorial Trial at 18-Month Follow-Up.

Loss of lean muscle mass impairs immunity and increases mortality risk among individuals with HIV/AIDS. We evaluated the relative contributions of protein supplementation and nutrition education on body composition among 600 women living with HIV/AIDS in rural Andhra Pradesh, India. We conducted a cluster randomized controlled 2 × 2 factorial trial lasting six months with follow up at twelve and eighteen months. Interventions occurred in the Nellore and Prakasam regions of Andhra Pradesh by trained village women, ASHA (Accredited Social Health Activists), and included: (1) the usual supportive care from ASHA (UC); (2) UC plus nutrition education (NE); (3) UC plus nutritional protein supplementation (NS); (4) combined UC plus NE plus NS. A Bioimpedance Analyzer Model 310e measured body composition. SAS 9.4 analyzed all data. Mixed models using repeated measures evaluated lean mass change from baseline as primary and fat weight and total weight as secondary outcomes. Lean mass change was significantly associated with NS (p = 0.0001), NE (p = 0.0001), and combined NS plus NE (p = 0.0001), with similar associations for secondary outcomes. Stronger associations for total weight were observed with greater ART adherence. Nutritional interventions may improve physiologic response to HIV. Significant increases in lean mass resulted from independent and combined protein supplementation and nutrition education.

Whey protein and vitamin D supplementation in institutionalized older adults: A randomized trial.

Background: The increase in life expectancy and in the number of individuals over 60 years old brings new demands to health professionals and services based on the physiological changes that occur in this population. The aging process results in changes in body composition, increasing body fat and reducing muscle mass, in addition to a reduction in bone mass. Aim: The aim of this study was to examine the effect of whey protein and vitamin D supplementation on body composition and skeletal muscle in older adults living in long-term care facilities. Methods: This study is a double-blind randomized controlled trial. Thirty older adults (>60 years old) were randomized and allocated in three groups: group receiving resistance training and supplementation receiving resistance training, whey protein and vitamin D; group received resistance and placebo training receiving resistance training and placebo, and control group without any intervention. Body composition was measured by dual-energy X-ray absorptiometry at baseline, 12 weeks, and 24 weeks. Results: The mean age was 74.87 (± 8.14) years. A significant difference (p = 0.042) was observed between the group receiving resistance training and supplementation and control groups in relation to lean mass increase (kg) at 24 weeks. After 24 weeks of intervention, there was a significant increase in Relative index of muscle mass for the two groups that underwent resistance training, group received resistance and placebo training (p = 0.042) and group receiving resistance training and supplementation (p = 0.045), in relation to the control. Conclusion: Combined supplementation of whey protein and vitamin D with resistance training can significantly improve lean mass, total mass, and relative index of muscle mass in institutionalized older adults.

Children’s Changes in Perceptions and Body Composition Following a Family-Based Fitness Intervention

Physical fitness is associated with decreased weight in children, which helps improve youth obesity rates. Family programs can provide practical approaches to improving physical fitness for children. The purpose of this study was to assess the influence of a family-based fitness intervention on changes in body composition, fitness status, and perceptions of obese children. Families attended exercise and education sessions for 60–90 min once per week for 10 weeks. Participants consisted of 10 children who participated in semi-structured interviews, body composition, and fitness assessments at pre- and posttest. Following the intervention, results showed that children had a significant increase in sit-ups (p = .03, effect size = 0.79), lean mass (p &lt; .001, Cohen’sd = 1.95), and bone mineral content (p &lt; .001, Cohen’sd = 0.46). Using thematic analysis, four main themes emerged from the pre- and postintervention interviews: an increase in after-school and weekend intentional physical fitness, rules regarding sedentary behavior and screen time, more prevalent physical fitness reinforcements, and a shift in perception of exercise. Results from this study indicate a family-based intervention had an influence on children’s appreciation for and engagement in physical fitness, as well as healthy body composition and fitness outcomes.

Evaluating the Effects of Increased Protein Intake on Muscle Strength, Hypertrophy and Power Adaptations with Concurrent Training: A Narrative Review.

Concurrent training incorporates dual exercise modalities, typically resistance and aerobic-based exercise, either in a single session or as part of a periodized training program, that can promote muscle strength, mass, power/force and aerobic capacity adaptations for the purposes of sports performance or general health/wellbeing. Despite multiple health and exercise performance-related benefits, diminished muscle hypertrophy, strength and power have been reported with concurrent training compared to resistance training in isolation. Dietary protein is well-established to facilitate skeletal muscle growth, repair and regeneration during recovery from exercise. The degree to which increased protein intake can amplify adaptation responses with resistance exercise, and to a lesser extent aerobic exercise, has been highly studied. In contrast, much less focus has been directed toward the capacity for protein to enhance anabolic and metabolic responses with divergent contractile stimuli inherent to concurrent training and potentially negate interference in muscle strength, power and hypertrophy. This review consolidates available literature investigating increased protein intake on rates of muscle protein synthesis, hypertrophy, strength and force/power adaptations following acute and chronic concurrent training. Acute concurrent exercise studies provide evidence for the significant stimulation of myofibrillar protein synthesis with protein compared to placebo ingestion. High protein intake can also augment increases in lean mass with chronic concurrent training, although these increases do not appear to translate into further improvements in strength adaptations. Similarly, the available evidence indicates protein intake twice the recommended intake and beyond does not rescue decrements in selective aspects of muscle force and power production with concurrent training.

Abstract 11483: A 7-Year Arterial Stiffness and Carotid Intima-Media Thickness Progression Predict Obesity, High Lean Mass, and Incident Hypertension from Age 17.7 to 24.5 Years: the ALSPAC Study

Introduction: An American Heart Association’s statement recommended investigating the natural history of arterial stiffness and blood pressure (BP) vis-à-vis the rate at which arterial stiffness and BP increase with age. Hypothesis: To test whether carotid-femoral pulse wave velocity (cfPWV), a measure of arterial stiffness, and carotid intima-media thickness (cIMT) progression independently predict increased fat mass and BP. Methods: We studied 3862 participants from the Avon Longitudinal Study of Parents and Children birth cohort. cfPWV and cIMT were measured by ultrasound scan, total and trunk fat mass, and lean mass by dual-energy Xray absorptiometry scan, body mass index (BMI), BP, heart rate, fasting insulin, glucose, high sensitivity C-reactive protein, and lipid profile were measured at ages 17.7 and 24.5 years. Participants at &gt;75th percentile of total and trunk fat mass or having &gt;24.9 kg/m 2 BMI were classified as overweight and obese, &gt;75th percentile as high lean mass, and &gt;120/80 mm Hg as elevated BP and hypertension. Data were analysed with logistic regression and linear mixed models, adjusting for cardiometabolic and lifestyle factors. Results: Among 1719 [44.5%] male and 2143 [55.5%] female participants, cfPWV at 17.7 years independently predicted systolic hypertension [Odds ratio (OR) 1.19 (1.01 - 1.40); p=0.043], diastolic hypertension [OR 1.77 (1.32 - 2.38); p&lt;0.0001], BMI-obesity [OR 1.19 (1.01 - 1.41); p=0.041], and trunk fat mass-obesity [OR 1.24 (1.03 - 1.49); p=0.023] at age 24.5 years. cIMT at 17.7 years predicted high lean mass [OR 54.29 (0.73 - 4053.52); p=0.069] at 24.5 years with borderline significance. A 7-year increase in cfPWV was directly associated with the 7-year increase in lean mass [Effect estimate 0.09 kg (0.05 - 0.13); p&lt;0.0001], systolic BP [21.58 mmHg (14.32 - 28.84); p&lt;0.0001] and diastolic BP [31.74 mmHg (24.90 - 38.57); p&lt;0.0001], but inversely associated with BMI [-0.03 kg/m 2 (-0.06 - -0.01); p=0.039]. cIMT progression from age 17.7 - 24.5 years was directly associated with the 7-year increase in total fat mass, trunk fat mass, lean mass, BMI, and diastolic BP. Conclusions: Arterial stiffness and cIMT progression independently predicted obesity, high lean mass, and incident hypertension among young adults.

Dairy intake and long-term body weight status in German children and adolescents: results from the DONALD study

PurposeTo analyse the association between intake of total dairy (TD) and types of dairy [liquid dairy (LD), solid dairy (SD), low-fat dairy (LFD), high-fat dairy (HFD), high sugar dairy (HSD), low-sugar dairy (LSD), not fermented dairy (NFD), as well as fermented dairy (FD)] and long-term changes in body weight status and composition among children and adolescents in Germany.MethodsIn total, 9999 3-day dietary records collected between 1985 and 2019 by 1126 participants (3.5–18.5 years; boys: 50.8%) of the Dortmund Nutritional and Anthropometric Longitudinally Designed (DONALD) study were analysed. Polynomial mixed-effects regression models were used to examine whether changes (median follow-up: 9 years) in the intake of TD and dairy types (in 100 g/1000 kcal total energy intake) were associated with changes in body-mass-index-standard-deviation-score (BMI-SDS); fat mass index (FMI); fat-free mass index (FFMI) over time.ResultsAn individual increase in TD intake was slightly but significantly associated with an increase in BMI-SDS (β = 0.0092; p = 0.0371), FMI (β = 0.022; p = 0.0162), and FFMI (β = 0.0156; p = 0.0417) after adjustment for potential confounder. Analyses for LD (BMI-SDS: β = 0.0139; p = 0.0052; FMI: β = 0.0258; p = 0.0125; FFMI: β = 0.0239; p = 0.0052) and LSD intake (BMI-SDS: β = 0.0132; p = 0.0041, FMI: β = 0.02; p = 0.0316, FFMI: β = 0.0183; p = 0.0189) showed similar results to TD. Both processing method and fat content showed no association with body composition in our analyses.ConclusionIncreases in TD, LD, and LSD intake showed small but significant increases in BMI and concomitant increases in fat mass and lean mass. However, the observed changes were too small to expect biological or physiological meaningful effects. Overall, our results showed that policies to promote dairy intake in childhood are to be welcomed, as no negative effects on body composition are expected, while the intake of important nutrients for growth is ensured. The type of dairy does not seem to matter.

PSV-B-20 Effects of Saccharomyces Cerevisiae Fermentation Product (SCFP) postbiotic in labrador retrievers during exercise and transport stress

Abstract Saccharomyces cerevisiae fermentation product (SCFP) is a dietary postbiotic compound shown to support digestive health and immune function in several species. Two studies were performed to evaluate the effects of SCFP (Diamond V Mills, Inc.; Cedar Rapids, IA) in canines exposed to exercise and transport stress. In both studies, 36 Labrador Retrievers (18 males/18 females) &amp;gt;5y were used. Study 1 consisted of a 3-wk adaptation period followed by a 14-wk series of distance-defined running exercise regimens (DDER) with subjects randomly allocated to three groups of 0 mg/d SCFP, 250 mg/d SCFP, or 500 mg/d SCFP. Study 2 consisted of a 3-wk adaptation period followed by an 8 wk phase of DDER and a final, single transport stress event. Subjects were randomly allocated to two treatments of 0 mg/d SCFP and 250 mg/d SCFP. Parameters examined over the two studies included body composition, body weight, feed intake, activity, running speed, stool quality. The following serum biomarkers were also measured: saliva cortisol (CORT), total antioxidant capacity (TAC), thiobarbituric acid reactive substances (TBARS), c-reactive protein (CRP), creatine kinase (CK), haptoglobin (HAP), serum amyloid A (SAA), TNF-α, immunoglobulin E (IgE), and immunoglobulin G (IgG). Subjects fed SCFP exhibited numeric net increases in lean mass (Table 1), numeric increases in stool quality, and significantly higher activity during exercise (P &amp;lt; 0.05) vs CON (Table 2). The 500 mg/d SCFP group exhibited lower CK (P &amp;lt; 0.03), increased HAP (P &amp;lt; 0.001), increased SAA (P &amp;lt; 0.05), increased TNF-α (P &amp;lt; 0.007), increased CORT, improved TAC (P &amp;lt; 0.001), reduced TBARS (P &amp;lt; 0.01), IgE (P &amp;lt; 0.001) and IgG (P &amp;lt; 0.001), and increased CRP during transport (P &amp;lt; 0.001) (Table 3). These results demonstrate that Labrador Retrievers supplemented with SCFP may exhibit improved vitality, utilization of nutrients, immune function, and reduced cellular damage when subjected to exercise and transport stress.