Increase In Iron Load Research Articles

Kinetics, Isotherm, and Thermodynamic Study for Ultrafast Adsorption of Azo Dye by an Efficient Sorbent: Ternary Mg/(Al + Fe) Layered Double Hydroxides.

The extremely high adsorption efficiency of malachite green (MG) was examined through a series of batch experiments by using Fe3+-doped Mg/Al layered double hydroxides (LDHs). The incorporation of iron into Mg/Al LDH with varying Al + Fe molar ratio of 4 + 1, 3 + 2, 2 + 3, and 1 + 4 increased the adsorption capacity with respect to time. The spectral analysis and N2 sorption studies showed that there was retention of surface morphology in all of the iron-modified LDH samples. The experimental evidences showed that the adsorbent Mg/(Al + Fe) with a molar ratio of 10:2 + 3 had a significant removal, i.e., 99.94% for MG with the initial concentration of 1000 mg L–1 at pH ∼ 9 and at room temperature in 5 min. With further increase in iron loading (at ratio 10:1 + 4), there was a decrease in the removal of MG due to the agglomeration of Fe2O3 on the surface. The adsorption process was best fitted to the Freundlich isotherm followed by the pseudo-second-order model. The standard thermodynamic parameters (ΔH°, ΔS°, and ΔG°) were obtained over the temperature range of 20–50 °C. It was observed that the adsorption of MG onto Mg/(Al + Fe) LDH was spontaneous, exothermic, and enthalpy driven in the physisorption mode. A worthy desorption efficiency was achieved by using ethanol and water, which was more than 90% in the three cycles. Maintaining almost the same removal efficiency of MG even after three cycles indicated Mg/(Al + Fe) LDH as a promising material for wastewater treatment. This work was anticipated to open up new possibilities in dealing with anionic dye pollutants.

Biomonitorization of iron accumulation in the substantia nigra from Lewy body disease patients.

Iron levels in the healthy human brain are known to be high in certain areas such as the substantia nigra (SN), and increase further with age. In addition, there is some evidence for a further increase in iron load in the SN of Parkinson's disease (PD) patients as compared to controls, which correlates with motor disability. Here, we have analyzed total iron levels in cells as well as mouse and human brain samples by atomic absorption spectroscopy (AAS). Our data indicate that iron load is more pronounced in cells with dopaminergic features. Moreover, region-specific differences in iron load reflecting those in the human brain were detected in rodent brains as well. Whilst altered iron load was not observed in other regions also affected in PD patients, we report a significant increase in iron load in the SN of Lewy body disease patients as compared to Alzheimer's disease (AD) patients or controls, which correlates with neurodegeneration in this brain area.

Preclinical toxicity of AZD7969: Effects of GSK3β inhibition in adult stem cells.

AZD7969 is a potent inhibitor of glycogen synthase kinase 3 (GSK3β), which is a multifunctional serine/threonine kinase that negatively regulates the Wnt/β-catenin signaling pathway. Treatment of rats and dogs with AZD7969 for periods of up to 4 weeks resulted in a number of changes, the most significant of which was a dose-dependent, and treatment-related, increase in proliferation in a number of tissues that was thought to arise from derepression of Wnt/β-catenin signaling in the stem cell compartment. Phenotypically, this resulted in hyperplasia that either maintained normal tissue architecture in the gastrointestinal tract, liver, kidney, and adrenals or effaced normal tissue architecture within the bones, incisor teeth, and femorotibial joint. In addition to these changes, we noted a treatment-related increase in iron loading in the liver and proximal small intestines. This off-target effect was robust, potent, and occurred in both dogs and rats suggesting that AZD7969 might be a useful tool compound to study iron storage disorders in the laboratory.

Brain iron overload, insulin resistance, and cognitive performance in obese subjects: a preliminary MRI case-control study.

The linkage among the tissue iron stores, insulin resistance (IR), and cognition remains unclear in the obese population. We aimed to identify the factors that contribute to increased hepatic iron concentration (HIC) and brain iron overload (BIO), as evaluated by MRI, and to evaluate their impact on cognitive performance in obese and nonobese subjects. We prospectively recruited 23 middle-aged obese subjects without diabetes (13 women; age 50.4 ± 7.7 years; BMI 43.7 ± 4.48 kg/m2) and 20 healthy nonobese volunteers (10 women; age 48.8 ± 9.5 years; BMI 24.3 ± 3.54 kg/m2) in whom iron load was assessed in white and gray matter and the liver by MRI. IR was measured from HOMA-IR and an oral glucose tolerance test. A battery of neuropsychological tests was used to evaluate the cognitive performance. Multivariate regression analysis was used to identify the independent associations of BIO and cognitive performance. A significant increase in iron load was detected at the caudate nucleus (P < 0.001), lenticular nucleus (P = 0.004), hypothalamus (P = 0.002), hippocampus (P < 0.001), and liver (P < 0.001) in obese subjects. There was a positive correlation between HIC and BIO at caudate (r = 0.517, P < 0.001), hypothalamus (r = 0.396, P = 0.009), and hippocampus (r = 0.347, P < 0.023). The area under the curve of insulin was independently associated with BIO at the caudate (P = 0.001), hippocampus (P = 0.028), and HIC (P = 0.025). BIOs at the caudate (P = 0.028), hypothalamus (P = 0.006), and lenticular nucleus (P = 0.012) were independently associated with worse cognitive performance. Obesity and IR may contribute to increased HIC and BIO being associated with worse cognitive performance. BIO could be a potentially useful MRI biomarker for IR and obesity-associated cognitive dysfunction.

Investigation of in Vivo Diferric Tyrosyl Radical Formation in Saccharomyces cerevisiae Rnr2 Protein

The β(2) subunit of class Ia ribonucleotide reductase (RNR) contains a diferric tyrosyl radical cofactor (Fe(2)(III)-Tyr(•)) that is essential for nucleotide reduction. The β(2) subunit of Saccharomyces cerevisiae is a heterodimer of Rnr2 (β) and Rnr4 (β'). Although only β is capable of iron binding and Tyr(•) formation, cells lacking β' are either dead or exhibit extremely low Tyr(•) levels and RNR activity depending on genetic backgrounds. Here, we present evidence supporting the model that β' is required for iron loading and Tyr(•) formation in β in vivo via a pathway that is likely dependent on the cytosolic monothiol glutaredoxins Grx3/Grx4 and the Fe-S cluster protein Dre2. rnr4 mutants are defective in iron loading into nascent β and are hypersensitive to iron depletion and the Tyr(•)-reducing agent hydroxyurea. Transient induction of β' in a GalRNR4 strain leads to a concomitant increase in iron loading and Tyr(•) levels in β. Tyr(•) can also be rapidly generated using endogenous iron when permeabilized Δrnr4 spheroplasts are supplemented with recombinant β' and is inhibited by adding an iron chelator prior to, but not after, β' supplementation. The growth defects of rnr4 mutants are enhanced by deficiencies in grx3/grx4 and dre2. Moreover, depletion of Dre2 in GalDRE2 cells leads to a decrease in both Tyr(•) levels and ββ' activity. This result, in combination with previous findings that a low level of Grx3/4 impairs RNR function, strongly suggests that Grx3/4 and Dre2 serve in the assembly of the deferric Tyr(•) cofactor in RNR.

Removal of hexavalent chromium in soil and groundwater by supported nano zero-valent iron on silica fume

Silica fume supported-Fe(0) nanoparticles (SF-Fe(0)) were prepared using commercial silica fume as a support. The feasibility of using this SF-Fe(0) for reductive immobilization of Cr(VI) was investigated through batch tests. Compared with unsupported Fe(0), SF-Fe(0) was significantly more active in Cr(VI) removal especially in 84 wt% silica fume loading. Silica fume had also been found to inhibit the formation of Fe(III)/Cr(III) precipitation on Fe nanoparticles' surface, which was increasing the deactivation resistance of iron. Cr(VI) was removed through physical adsorption of Cr(VI) onto the SF-Fe(0) surface and subsequent reduction of Cr(VI) to Cr(III). The rate of reduction of Cr(VI) could be expressed by pseudo first-order reaction kinetics. The rate constant increased with the increase in iron loading but decreased with the increase in initial Cr(VI) concentration. Furthermore, column tests showed that the SF-Fe(0) could be readily transported in model soil.

Reduction of Hexavalent Chromium in Aqueous Medium with Zerovalent Iron

Hexavalent chromium, emanating primarily from the tannery and electroplating industries, can be reduced to the less toxic trivalent variety by several methods, including reduction with metallic iron. In the present work, electrolytic-grade iron dust was used to reduce chromium(VI) in the form of potassium dichromate. Loading of iron dust was varied from 0.5 to 1.0 g in 50 mL of solution; the pH of the medium was varied from 1.5 to 3.5; and the initial concentration of the dichromate solution was varied from 50 to 125 mg/L. Under the specified experimental conditions, maximum removal of the hexavalent chromium achieved was approximately 90% of its original value. The time-concentration data followed a pseudo-first-order kinetic model. The conversion and rate of reduction increased with an increase in iron loading and acidity of the medium, whereas an increase in the initial concentration of chromium(VI) caused a decrease in the reduction.

Hexavalent chromium removal from water using chitosan-Fe0 nanoparticles

Chitosan-Fe0 nanoparticles (chitosan-Fe0) were prepared using nontoxic and biodegradable chitosan as a stabilizer. Laboratory batch and column experiments were conducted to investigate the feasibility of using chitosan-Fe0 for in situ reductive removal of Cr(VI) from water. Batch kinetic tests indicated that the overall disappearance of Cr(VI) may include both physical adsorption of Cr(VI) onto the chitosan-Fe0 surface and subsequent reduction of Cr(VI) to Cr(III). The rate of reduction of Cr(VI) to Cr(III) can be expressed by a pseudo-first-order reaction kinetics and the rate constants increase with the increase in iron loading. Column experiments indicated that the chitosan-Fe0 were highly deliverable in the column. When the column was treated with Cr(VI) -contaminated deionized water, the removal capacity of chitosan-Fe0 was found to be about 32 mg Cr/g Fe(0). A 25% drop in the Cr(VI) removal capacity was found when treated with Cr(VI)-contaminated surface water.

Full phenol peroxide oxidation over Fe-MMM-2 catalysts with enhanced hydrothermal stability

Iron-containing mesoporous mesophase materials Fe-MMM-2 have been synthesized by a sol–mesophase route under mild acidic conditions and characterized by DRS-UV–vis, XRD, and N2 adsorption measurements. It was found that pH of the synthesis solution and iron content in the samples affect both the textural characteristics and the state of iron atoms. Isolated iron species predominate in silica framework under Fe<2wt% and pH∼1.0 or Fe∼1wt% and pH<2.0. These species are stable to leaching and highly active in full H2O2-based phenol oxidation. The increase in iron loading and pH of the synthesis solution lead to the agglomeration and formation of oligomeric iron species, which, in turn, results in the reduction of the catalytic activity of Fe-MMM-2 and the increase of iron leaching.

Intensive Transfusion Therapy in Thalassemia Major

A hemoglobin level of over 10 gm per 100 ml has been maintained by transfusion in six children with thalassemia major. Chelating agents have been used to minimize the increase in iron loading. Striking clinical improvement with regression of abnormal cardiac symptoms and signs was seen in all these patients. It is concluded that, in thalassemia major, this therapy provides at least short-term clinical benefits to severely affected children. The rationale for long-term, intensive transfusion therapy is based on the assumption that it is the degree of anemia rather than the degree of iron loading which leads to the eventual cardiac failure and death in these patients. Proof of this hypothesis will depend to some extent on the subsequent clinical course of these children.