Continuous exposure to ozone (O 3 , 1.2 ppm) in adult CD-1 male mice for 20 consecutive days markedly influenced a number of items of aggressive behavior induced by 59 days of individual housing. The behavior of mice was videotaped on days 1, 3, and 5 of five consecutive daily encounters (10 min each ; isolation days 55, 57, and 59). Ozone exposure caused an abatement of aggressive behavior and enhanced fear-associated displays shown by a significant decrease in the frequency of attacking and digging and an increase in freezing. Moreover, 03 induced a decrease of time spent in attacking and exploration/activity. Nonexposed animals exhibited a day-dependent increase in self-grooming frequency which was not observed in 03 mice ; by contrast, freezing was higher in O 3 mice on day 5 of the test. Data suggest that 03 exposure produces a remarkable reduction of mouse aggression, indirectly confirming previous findings on physical and neurobehavioral effects of 03