PURPOSE: Verify if athletes undergone Cr supplementation and intense resistance training would show increase of free radical production. METHODS: 29 male elite handball athletes from Sorocaba/SP (17.1 + 1.63 years) were divided into three groups: GC (N=10) they are constituted by individuals supplemented with creatine; GP (N=10) is the placebo group, and COT (N=9) is constituted by control athletes. The Cr supplementation was performed during 32 days. All individuals were undergone a resistance training program, which concomitantly began to Cr supplementation. At the beginning and at the end of supplementation period, blood samples were drawn for the analysis of three important makers of oxidative stress: malondialdehyde (MDA), total antioxidant status (TAS), and uric acid. Fitness tests were also performed involving body composition, maximal repetition (RM), and muscular strength while performing bench press. All subjects agreed in taking part in this research and signed up the Informed Consent. Test “t” of paired Student was performed as Statistical analyses. RESULTS: The main revealed results related to physical fitness were that, it was only noticed significant statistical differences on RM which was increased in CG group (52.67 +8.90 vs., 62.50 + 9.77 kg p <0,05). No differences were seen (noticed) on MDA levels in any of the groups studied, though. However levels of acid uric increased in all groups-GC (4.6 ± 1.0 vs., 7.4 ± 1.6 mg/dl p <0,01); GP (4.4 ± 1.1 vs., 6.7 ±2.3 mg/dl p <0,05); COT (5.1 ±0.9 vs., 6.7 ± 1.2 mg/dl p <0,01) - and such data suggested us ischemic-reperfusion syndrome induced by resistance training. Finally, it was observed that TAS only decrease significantly on CG (1.11 ±0.34 vs., 0.6 ±0.19 mmol/l p <0,01). CONCLUSIONS: We conclude that in this group, the Cr supplementation promoted a significant increase on muscular strength, on the other hand, these subjects (CG) presented a significant decrease in TAS indicating an over consumption of antioxidant reserves in order to defend themselves of free radical.