Increase In Creatinine Concentration Research Articles

The Impact of Prenatal Alcohol Exposure on the Autonomic Nervous System and Cardiovascular System in Rats in a Sex-Specific Manner.

Fetal Alcohol Spectrum Disorder (FASD) is a consequence of prenatal alcohol exposure (PAE) associated with a range of effects, including dysmorphic features, prenatal and/or postnatal growth problems, and neurodevelopmental difficulties. Despite advances in treatment methods, there are still gaps in knowledge that highlight the need for further research. The study investigates the effect of PAE on the autonomic system, including sex differences that may aid in early FASD diagnosis, which is essential for effective interventions. During gestational days 5 to 20, five pregnant female Wistar rats were orally administered either glucose or ethanol. After 22 days, 26 offspring were born and kept with their mothers for 21 days before being isolated. Electrocardiographic recordings were taken on the 29th and 64th day. Heart rate variability (HRV) parameters were collected, including heart rate (HR), standard deviation (SD), standard deviation of normal-to-normal intervals (SDNN), and the root mean square of successive differences between normal heartbeats (RMSSD). Additionally, a biochemical analysis of basic serum parameters was performed on day 68 of the study. The study found that PAE had a significant impact on HRV. While electrolyte homeostasis remained mostly unaffected, sex differences were observed across various parameters in both control and PAE groups, highlighting the sex-specific effects of PAE. Specifically, the PAE group had lower mean heart rates, particularly among females, and higher SDNN and RMSSD values. Additionally, there was a shift towards parasympathetic activity and a reduction in heart rate entropy in the PAE group. Biochemical changes induced by PAE were also observed, including elevated levels of alanine transaminase (ALT) and aspartate aminotransferase (AST), especially in males, increased creatinine concentration in females, and alterations in lipid metabolism. PAE negatively affects the development of the autonomic nervous system, resulting in decreased heart rate and altered sympathetic activity. PAE also induces cardiovascular abnormalities with sex-specific effects, highlighting a relationship between PAE consequences and sex. Elevated liver enzymes in the PAE group may indicate direct toxic effects, while increased creatinine levels, particularly in females, may suggest an influence on nephrogenesis and vascular function. The reduced potassium content may be linked to hypothalamus-pituitary-adrenal axis overactivity.

Clinical profile of pediatric acute renal failure patients requiring renal replacement therapy age less than 15 years: an ambivalent COHORT study

Background: Acute kidney injury (AKI) is characterized by a reversible increase in the blood concentration of creatinine and nitrogenous waste products and by the inability of the kidney to regulate fluid and electrolyte homeostasis appropriately. There is minimal data on the clinical profile of pediatric AKI patients age less than 15 years who required renal replacement therapy (RRT). Methods: A total of 301 patients (3%) were diagnosed with AKI, amongst them 66 patients who required RRT from January 2021 to December 2022 were enrolled. Their clinical, biochemical, and etiological profile were studied to find out their influence in modifying the outcome of AKI patients requiring RRT. Results: AKI was noted in 301 patients (3%), amongst them 66 patients we had analyzed who underwent RRT. Result were analyzed in three age group (0-2 month, 2 month-8 year, 8 year-15 year). Male preponderance was seen amongst all age groups with male: female ratio being 2:1, 5:1 and 1.8:1 respectively and rural area preponderance seen in all age groups. Birth asphyxia, neonatal sepsis and septicemia were leading etiologies in different groups. Peritoneal dialysis (PD) was only RRT modality in the 0-2 month age group, while hemodialysis and PD modalities were used in rest groups. Multi-organ dysfunction syndrome was commonly seen comorbidity associated with all groups along with pyogenic meningitis, and encephalopathy. Mortality rate was observed at 47% (n=31). Conclusions: From our study, we conclude that to expand the facility of point of care, hemodialysis facility in ICUs with a larger study or multicentric study in the pediatric age group is required.

Investigation of Hepatic and Renal Parameters Content in Blood Samples of Snuff Addicted Individuals in Gusau Metropolis

In Nigeria, the use of dry nasal snuff is spreading considerably throughout all age groups in both rural and urban areas. This is due to various ethno-cultural beliefs that have not been scientifically confirmed. Various people do not realize the negative impact of snuff on one's health, such as liver and kidneys failure, elevation of blood pressure, and a higher chance of oral cancer. The hepatotoxicity and nephrotoxicity of the local nasal snuff on snuff addicted individual was poorly understood. This study was carried out on snuff addicted individuals to assess hepatotoxicity and nephrotoxicity of the local nasal snuff. One hundred and fifty individuals used in the study were group into three of fifty people each. Group one served as a control which include individuals who never use snuff, Groups two and three comprise individuals who are addicted to snuff, with ages ranging from 18 to 30, and above thirty, respectively. The hepatotoxicity study revealed that, there were significant (p<0.05) increase in concentration of ALT, AST, Total Bilirubin and Direct Bilirubin of group two and group three compared with control. The concentration of ALT, AST, Total Bilirubin and Direct Bilirubin of group three were also significantly (p<0.05) higher than that of group two. While there were significant (p<0.05) decrease in concentration of total protein and albumin in group two and three compared with control. The nephrotoxicity study revealed that, there were significant (p<0.05) increase in concentration of creatinine of group two and group three compared with control. The concentration of creatinine in group three was even significantly higher than that of group two. Whereas, there were significant (p<0.05) decrease in concentration of Urea, Sodium, Potassium and Chlorine in group two and three compared with control. This study was concluded that, there was health risks (Hepatotoxicity and Nephrotoxicity) associated with snuff addiction.

Creatinine recovery from bovine urine under the effect of different times and temperatures of storage.

Creatinine is a urinary marker used widely in ruminant's experimental trials. However, despite its great importance no data were found in the literature about the best way to store bovine urine samples. In the sheep urine, was observed an increase in the urinary concentration of creatinine when it was stored acidified (pH 2.5 to 3.5) at a temperature of 28 to 39 °C for 150 days of storage. Nevertheless, urine should be stored acidified (pH below 3) to avoid purine derivative degradation, So, aimed to evaluate creatinine recovery in bovine urine as a function of storage time and temperature. A total of 25 animals' urine (10 Nellore cattle and 15 Holstein cattle) were collected. The urine (40 mL) was diluted in 160 mL of distilled water and its pH was corrected to a value lower than 3 using sulfuric acid drops. A sample of the diluted urine was analyzed to obtain the creatinine concentration reference value on the collection day. The remaining urine was fractionated and preserved at room temperature, cooled (4 °C) or frozen (-20 °C and -40 °C). In the urine of five Holstein cattle was added creatine solutions (20, 40 and 60 mg/dL) to evaluate the creatine to creatinine conservation. These urine samples were analyzed on different days after collection (1, 3, 7, 15, 30 and 45 days). The urine without any added creatine was analyzed on Days 1, 3, 7, 10, 15, 30, 45, 60, 90, 120, and 150 of storage. The addition of creatine in the urine caused an increase in the creatinine concentration (P < 0.05) after 30 days of storage at room temperature and under refrigeration (4 °C). In frozen samples, there was no change in creatinine concentration (P > 0.05). However, creatinine recovery was constant (P > 0.05) until day 15 of storage, regardless of the temperature used, when creatine was not added. After 30 days of storage, an effect of time and/or temperature was observed on creatinine recovery (P < 0.05). Urine samples can be stored at any temperature for up to 15 days after collection to estimate the creatinine concentration. Samples that need storage times longer than 15 days should be frozen (at -20 °C and -40 °C) to avoid creatinine concentration variation.

Protective and Curative Potential of Ethanol Leaf Extract of Corchorus olitorius against Potassium Bromate-Induced Renal Toxicity

Aim: The objective of this study was to assess the protective and curative potential of ethanol leaf extract of Corchorus olitorius against potassium bromate (KBrO3)-induced renal toxicity.&#x0D; Methodology: Corchorus olitorius was extracted using a soxhlet extractor and ethanol as the solvent. After becoming accustomed to the lab, 24 mature male Wistar rats were randomly assigned to groups A, B, C, and D. Group A received oral distilled water as treatment. Animals in groups B, C, and D got 100 mg/kg body weight of potassium bromate while groups C and D also received 100 and 200 mg/kg body weight of Corchorus olitorius respectively. Fresh potassium bromate and groups C and D extract were administered to rats every day by oral gavage. After taking the drug for the recommended 28 days, blood and kidney samples were collected. Renal biomarkers were evaluated using conventional methods.&#x0D; Results: Significant (P0.05) increase in the serum concentrations of creatinine, urea, uric acid, sodium (Na+), potassium (K+), chloride (Cl–), and bicarbonate (HCO3–) were observed following potassium bromate administration in comparison to the control group. KBrO3 poisoning also increased the levels of the inflammatory proteins interleukin-6 (IL-6) and tumour necrosis factor-alpha (TNF-) in the kidneys compared to the control group. Yet when KBrO3 and C. olitorius leaf extract were administered together, levels of all kidney indicators were significantly reduced in a dosage-dependent manner, with 200 mg/kg being the most efficient dose.&#x0D; Conclusion: This study found that C. olitorius leaf extract, particularly at the higher dose of 200 mg/kg, was successful in reducing a number of the parameters examined that had been negatively impacted by KBrO3. It may be advantageous to include C. olitorius leaf in edible products that may contain KBrO3, such as flour, bread, or cakes, as it is a well-known dietary prebiotic with established safety profiles in humans. Further research is required to determine whether C. olitorius leaves can reduce the toxicity of KBrO3 in human organs and other animal strains, as well as perhaps treat it.

Endogenous intoxication, oxidative stress and antioxidant system in acute lower limb ischemia

Aim. To study features of endogenous intoxication syndrome (EI), oxidative stress (LPO) and antioxidant defence (AOS) in patients with acute lower limb ischemia (LLI).Materials and methods. Some parameters of EI, LPO and AOS were studied. EI syndrome was studied in 86 patients with LLI who underwent complex examination and treatment in 2020-2021 in the Department of Vascular Surgery of the RNCfCS. POL and AOS parameters were studied in 14 patients out of 86. There were 51 (59.3%) men and 35 (40.7%) women, mean age was 56.0±5.8 years. Acute occlusion of the iliofemoral segments was noted in 33 (38.4%) patients, in 32 (37.2%) - the femoral segment, and in 21 (24.4%) - the popliteal and femoral vessels. In all cases, ischemia had a unilateral localization - on the right in 59 (68.6%) cases and on the left in 27 (31.4%) cases. In 52 (58.1%) cases, there were clinical signs of grade II b ischemia, and in 36 (41.9%) cases - grade III A (according to V.S. Saveliev, 1987). The average duration of ischemia was 77.9±28.3 hours.Results and discussion. In all cases of LLI before revascularization, there were signs of acute inflammatory reaction and EI syndrome in the form of increased ESR (42.3±7.1 mm/h), CRP concentration (16.1±4.2 mg/L), fibrinogen (8.5±0.9 g/L) and leukocytosis (12.7±1.5×109/L). Also, all patients had a slight elevation of blood glucose against the background of EI (7.5±1.8 mmol/L), a significant increase of creatinine concentration (128.9±6.1 µmol/L), urea (9.2±0.6 mmol/L) and aminotransferases as compared to normal indices. Results of LPO and AOS indices study in 14 patients showed that in LLI, malondialdehyde (MDA) increases 2.6-fold (3.9±0.1 μmol/L), diene conjugate increases 3.7-fold (0.77±0.04 units a/mL). However, after the elimination of ischemia, there was no significant decrease for 12.2±2.4 days, which confirms the presence of reperfusion syndrome. AOS indices - superoxide dismutase (20,8±1,0 u/l) and catalase (128,9±11,2 μl/l) increased 1,2 and 1,1 times in the examined patients cohort, respectively. Statistically significant associations were established between the following indices: Ischemia level and urea (r=0.89), Ischemia level and white blood cell count (r=-0.82), Hb and Er (r=0.99), Hb and creatinine (r=0.79), Hb and urea (r=0.79), Er and urea (r=0.79), creatinine and Er (r=0.90), ACTV and creatinine (r=-0.77), creatinine and urea (r=0.90).Conclusion. Acute lower limb ischemia is accompanied by ischemia-reperfusion damage manifested by endogenous intoxication syndrome, impaired glucose metabolism and activation of lipoperoxidation processes and antioxidant system. After the elimination of acute ischemia, there is not always a significant decrease in lipid peroxidation and some inflammation parameters. Therefore, prolonged use of antioxidants in the reperfusion period is recommended.

Re-treatment with [177Lu]Lu-DOTA-TATE or [177Lu]Lu-DOTA-TATE and [90Y]Y-DOTA-TATE of patients with progressive neuroendocrine neoplasm.

Neuroendocrine neoplasms (NENs) are heterogeneous groups of tumours derived from neuroendocrine cells of the ectoderm or endoderm. They are considered rare, with an estimated incidence and prevalence of 6/100,000 and 35/100,000 respectively, and a noticeable upward trend. Radioligand therapy (RLT) using beta-radiation-emitters combined with somatostatin analogues is an effective and relatively safe treatment method. It is usually used as a second-line therapy in case of progressive disease. In retrospective analysis covering eight years of observation (2015-2023) of patients treated in a single highest-reference NEN centre, a subgroup of 13 who received RLT re-treatment (¹⁷⁷Lu or ¹⁷⁷Lu/⁹⁰Y-mixture) was identified. Epidemiological aspects, renal, hepatic, haematological parameters and chromogranin A serum concentration were analysed. The median PFS after the first cycle of RLT was 53.8 months (IQR = 19.3). Directly after the second cycle of RLT disease stabilization and progression was observed in 11/13 (84.6%) and 2/13 (15.4%) patients respectively. After the second cycle of RLT median observation time for the study group was 16.2 months. Eight out of 13 patients were reachable for long-term observation and stabilization was confirmed in 62.5 % (5/8), progression in 12.5% (1/8) and death in 25% (2/8) patients. Median survival time in patients with confirmed death was 7 months. During observation, an increase in creatinine concentration with a decrease in glomerular filtration rate (GFR) was noticed, however, the values were at a statistical trend level (p = 0.056; p = 0.071). The increase of liver parameters was statistically, but not clinically significant. The decrease in albumin concentration and fasting glucose concentration were not significant. An increase in chromogranin A concentration correlated, although not statistically, with the progression of the disease. A statistically significant decrease in the number of all bone marrow cell lines was observed. The first RLT cycle caused a higher decrease in blood parameters than the second. There were no differences in PFS or laboratory parameters depending on the radioligand ([¹⁷⁷Lu]Lu-DOTA-TATE vs. [¹⁷⁷Lu]Lu-DOTA-TATE/[⁹⁰Y]Y-DOTA-TATE). In follow-up after RLT re-treatment stabilization was observed in 62.5%, progression in 12.5% and death in 25% of patients. Decrease of glomerular filtration, and bone marrow parameters resulted from the cumulative adverse effect of RLT, the natural ageing process, and the progression of the disease. Side effects were mainly caused by the first treatment cycle. There was no significant influence on the measured parameters, depending on the radioisotope used. Re-treatment of RLT seems to be a reliable and relatively safe method, thus should be considered in patients who underwent one cycle of RLT and responded to the treatment.

441 EFFECTS OF TRANSCATHETER AORTIC VALVE IMPLANTATION ON RENAL FUNCTION IN CT-DEFINED SARCOPENIC PATIENTS

Abstract Introduction Sarcopaenia, the age-associated decline in skeletal muscle and function, increases the risk of procedure-related complications and mortality in patients undergoing transcatheter aortic valve implantation (TAVI). We sought to investigate whether CT-determined sarcopenia impacts on in-hospital renal function and on midterm outcomes following TAVI. Methods This was a retrospective cohort study including patients who underwent TAVI. Sarcopaenia was CT-defined as skeletal muscle mass index &amp;lt;55.4 cm2/m2 in males and &amp;lt;38.9 cm2/m2 in females at the level of L3 vertebra. Acute kidney injury (AKI) was defined as an increase in creatinine concentration of at least 0.3 mg/dL within 48 hours of TAVI according to Valve Academic Research Consortium-3 Criteria. Acute kidney recovery (AKR) was determined as an increase of 25% in eGFR within 48 hours following TAVI. The primary endpoint was freedom from major adverse cardiac and cerebrovascular events (MACCE; composite of any cause of death, any coronary revascularization, stroke and heart failure hospitalization) at the 1-year follow up in sarcopaenic and non-sarcopaenic patients. Results A total of 113 patients (mean age 82.2 ± 4.03 years, 52.2% female) were followed for a median of 1.1 year. Sarcopenia was found in the 59% of the TAVI population, more frequently among males (72.2% versus 33.8%, p&amp;lt;0.01). The incidence of AKI was 8.0% and AKR was 6.9% without differences between sarcopaenic and non-sarcopaenic patients (9.4% versus 6.5%, p=0.59 and 11.6% versus 2.2%, p=0.08). The freedom from MACCE at 1 year follow up was 74.5% for sarcopaenic and 70.3% for non-sarcopaenic patients (p= 0.6). In sarcopaenic patients, the incidence of AKI and AKR had no significant impact on the freedom from MACCE at 1-year (p=0.58 and p=0.87). Conclusions Sarcopaenic patients had similar out of hospital incidence of adverse events to non-sarcopaenic patients following TAVI confirming the safety of this procedure in sarcopaenic patients.

Interference on the Abbott i-STAT creatinine assay caused by hydroxyurea

Abstract Serum creatinine is an important biomarker used for estimating glomerular filtration rate (eGFR). The present study was designed after a patient at our institution had a significantly elevated creatinine result taken from an i-STAT point of care test that utilizes electrochemical detection. The patient’s creatinine from the i-STAT was 3.8 mg/dL and was not consistent with their clinical history. The patient’s previous creatinine taken 2 days prior was 1.39 mg/dL and 1.27 mg/dL seven days prior. Both previous readings were measured with the Siemens Dimension Vista 1500 using a colorimetric enzymatic creatinine method. A subsequent creatinine measured on the Siemens Dimension Vista 1500 was 1.30 mg/dL. Upon chart review, the clinical team noted that the patient is taking 500 mg of hydroxyurea daily for treatment of a myeloproliferative disorder. Review of the i-STAT package insert revealed hydroxyurea as a known interfering substance and states for every 100 µmol/L hydroxyurea in the specimen, creatinine will be increased by approximately 1.85 mg/dL. Review of literature described the positive interference caused by hydroxyurea for the i-STAT creatinine and glucose assays but did not explain the mechanism of this positive interference. In order to characterize the mechanism of hydroxyurea interference, we investigated the situation further. A pool of plasma was spiked with hydroxyurea. The spiked pool was serial diluted (x2, x4, x8, x16, x32, x64, x128) to observe the creatinine results in the presence of and absence of hydroxyurea. The dilution series was analyzed using the Vista enzymatic creatinine and the Abbott i-STAT enzymatic creatinine methods. The i-STAT creatinine results show increasing creatinine concentration from interference as the concentration of hydroxyurea increased. The dilution series suggests increasing positive interference with hydroxyurea. Positive creatinine interference with hydroxyurea was not observed when analyzed on the Vista platform. Hydroxyurea is a widely used treatment for myeloproliferative disorders and also for managing sickle cell disease. Hydroxyurea is known to cause positive interference in the i-STAT enzymatic creatine method that uses electrochemical detection, with the degree of interference correlating with the concentration of hydroxyurea. The Vista colorimetric enzymatic creatinine method was not observed to have hydroxyurea interference. While both methods employ enzymatic reagent systems the final detection approach is important. Patients undergoing hydroxyurea treatment should avoid i-STAT measurement systems for creatinine.

Nephroprotective and Antioxidant Effects of Flavonoid-Rich Extract of Thymelaea microphylla Coss. et Dur Aerial Part

Thymelaea microphylla Coss. et Dur (T. microphylla), a medicinal herb that grows in arid and desert pastures, has been traditionally utilized by Moroccans to treat many diseases, including kidney failure. This study aims to evaluate the nephroprotective effect against gentamicin-induced nephrotoxicity (GM), and thus the antioxidant activity of an aqueous extract rich in flavonoids from the aerial part of T. microphylla (APTM). The antioxidant activity of APTM was assessed using the 2-2-diphenyl-1-picrylhydrazyl (DPPH) scavenging test and the β-carotene bleaching assay. The nephroprotective effect of this extract was evaluated in two doses of 200 and 400 mg/kg in GM-exposed male rats. Acute toxicity of the APTM was tested out on Swiss albino mice using 2000 mg/kg as the dose limit. The findings showed that the aqueous extract of APTM is abundant in total polyphenols and flavonoids and has significant antioxidant properties against DPPH radicals and β-carotene oxidation. According to the acute toxicity research findings, the administered doses of the APTM extract do not cause toxicity and death. A significant increase in the serum concentrations of creatinine, urea, uric acid, sodium, chloride, calcium, gamma-glutamyl transpeptidase (gamma-GT), and alkaline phosphatase, as well as an increase in urinary volume, water consumption, and relative kidney weight, were all caused by the administration of GM to rats. In addition, a significant reduction in urinary concentrations of creatinine, uric acid, urea, and albumin, and thus the clearance of creatinine and weight gain were observed in rats injected with GM. Also, the administration of GM dramatically raised the malondialdehyde level in the kidneys. Likewise, rats that had been poisoned with GM had histological kidney abnormalities. However, the daily treatment of APTM aqueous extract to rats given GM injections dramatically improved the biochemical and histological parameters affected by GM administration in rats. Finally, APTM extract enhanced GM’s biochemical and histological indicators of nephrotoxicity, supporting its use as an ethnomedicinal.

Evaluate the propylthiouracil on thyroid, liver and kidney functions in male rabbits

This work was conducted to investigate the effect of Propylthiouracil thyroid kidney and liver functions in male rabbits. Fifteen (15) rabbits were divided into three groups: a control group, which received tap water only, a low-dose group that received 50 mg/kg oral propylthiouracil and a high-dose group, which received 100 mg/kg oral propylthiouracil. Rabbits were dosed daily for three weeks. After the three-weeks period, the rabbits were sacrificed and blood samples were collected for hormonal and biochemical analysis. Showed changes in liver enzymes as a result of liver tissue damage. Propylthiouracil caused an increase in creatinine concentration, alkaline phosphatase levels, and liver enzyme levels. PTU treatment had no significant effect on the mean urea concentration level. In view of the changes summarized, the increase or decrease in this parameter arises from the reduction in the level of thyroid hormones which, in turn, affect most body tissues.

Evaluation of Some Hepato- Renal Biochemical Parameters in Typhoid Fever Patients in Yenagoa, Bayelsa State Nigeria

Introduction: Typhoid fever infection is one of the most prevalent diseases in developing countries. It is caused by Salmonella species and can affect multiple organs in the body if left untreated. The purpose of this study was to determine the biochemical patterns of renal and liver biochemical parameters in patients admitted with typhoid fever.&#x0D; Methodology: A total of 100 subjects were recruited for the study, with 50 typhoid-positive patients and 50 typhoid-negative individuals serving as controls. Blood samples collected from the subjects were analyzed for Salmonella Typhi titre, sodium (Na), potassium (K), chloride (Cl), urea, creatinine, aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) using WHO-approved methods. The data obtained were statistically evaluated in SPSS version 22.&#x0D; Results: The comparison between the positive and negative titres of Salmonella Typhi showed a significant increase in concentrations of creatinine and AST in the positive titres, whereas sodium decreased.&#x0D; Conclusion: This result suggests that patients with high titers of Salmonella Typhi are more susceptible to hepatocellular injury, muscle wasting, and hyponatremia. Therefore, liver and kidney function tests should be essential clinical tests for the management of typhoid fever.

The Clinical Impact of Platelets on Post-Injury Serum Creatinine Concentration in Multiple Trauma Patients: A Retrospective Cohort Study.

Background and objective: Platelets contribute to the immunological response after multiple trauma. To determine the clinical impact, this study analyzes the association between platelets and creatinine concentration as an indicator of kidney function in polytraumatized patients. Methods: We investigated all patients presenting an Injury Severity Score (ISS) ≥16 for a 2-year period at our trauma center. Platelet counts and creatinine concentrations were analyzed, and correlation analysis was performed within 10 days after multiple trauma. Results: 83 patients with a median ISS of 22 were included. Platelet count was decreased on day 3 (p ≤ 0.001) and increased on day 10 (p ≤ 0.001). Platelet count was elevated on day 10 in younger patients and diminished in severely injured patients (ISS ≥35) on day 1 (p = 0.012) and day 3 (p = 0.011). Creatinine concentration was decreased on day 1 (p = 0.003) and day 10 (p ≤ 0.001) in female patients. Age (p = 0.01), male sex (p = 0.004), and injury severity (p = 0.014) were identified as factors for increased creatinine concentration on day 1, whereas platelets (p = 0.046) were associated with decreased creatinine concentrations on day 5 after multiple trauma. Conclusions: Kinetics of platelet count and creatinine concentration are influenced by age, gender, and trauma severity. There was no clear correlation between platelet counts and creatinine concentration. However, platelets seem to have a modulating effect on creatinine concentrations in the vulnerable phase after trauma.

Science, Medicine, and the Anesthesiologist

Science, Medicine, and the Anesthesiologist

In silico drug-like properties prediction and in vivo antifungal potentials of Citrullus lanatus seed oil against Candida albicans

Citrullus lanatus seed is an important but neglected seed rich in essential fatty acids. The study sought to investigate in silico and in vivo antifungal activity of some bioactive compounds of Citrullus lanatus (watermelon) seed oil (CLSO) on oral candidiasis induced by Candida albicans in immunosuppressed female albino rats and to predict the Absorption, Distribution, Metabolism and Excretion (ADME) properties of isolated natural compound. Docking studies was performed using standard procedure, standard microbiological and histopathological techniques was employed for study of in vivo antifungal activity of the oil; as well as renal function tests at days 7 and 14 post-infection treatments. Treated groups were compared with that of the control groups. In vitro studies showed varied zone of inhibitions at different concentrations. Ligand-protein interaction showed better binding activity between Palmitic acid and SAP-5 as well as CYP51 when compared with fluconazole (reference drug). Treatment with CLSO showed that there was a significant reduction in the kidney fungal burden (cfu/ml/g) of rats treated with CLSO after 14 days post-infection treatment, compared to group 2 (untreated control) rats. Histomorphology of group 2 showed multifocal aggregation and widespread distribution of fungal blastospores when compared with CLSO-treated groups, which had minimal fungal blastospores in the renal tissues. Thus, in silico and histological data agreed with the findings in microbiology. Furthermore, within the CLSO treated group, a significant increase in the serum concentrations of creatinine was observed, while no significant difference in blood urea values was recorded after day 14 post-infection study. Linoleic and palmitic acid could be considered as a potential antifungal drug candidate with palmitic acid playing a significant role.

Optimisation of fluconazole therapy for the treatment of invasive candidiasis in preterm infants

IntroductionFluconazole is an important antifungal in the prevention and treatment of invasive Candida infections in neonates, even though its use in preterm infants is still off-label. Here, we performed a...

Morphological indicators of blood in cats with panleucopenia

Ukraine is one of the world leaders in the number of cats among the country's population, and one of the most common diseases is panleukopenia caused by a virus of the Parvoviridae family, which is extremely common among viral diseases and causes significant moral damage to owner animals. Therefore, given the relevance of this issue, the result of our study is to clarify, supplement and summarize the data on hematological and biochemical parameters of blood and erythrocytopoiesis in panleukopenia of cats. The development and improvement of modern methods of immunoprophylaxis and treatment can significantly reduce morbidity and mortality. The study was performed in veterinary clinics of Zhytomyr and Kyiv, blood for the study was taken from the superficial vein of the forearm Anterbrachium, medial subcutaneous vein or subcutaneous tibial vein Saphena. Diagnostic tests to confirm feline parvovirus were performed using rapid tests VetExpert and ELISA and PCR for the presence of parvovirus antigen in private veterinary laboratories “BioSoft” and LLC “Bald” in Kiev. The complex studies carried out indicated that in cats (n = 24) with panleukopenia, changes in the morphological and biochemical composition of the blood are characteristic. A deviation from physiological limits was found, which mainly characterize the functional state of the liver, namely, an increase in the content of total bilirubin (P ≤ 0.01) and total protein (P ≤ 0.01), the development of pancreatitis – taking into account an increase in glucose concentration (P ≤ 0.05) and α-amylase hyperenzymemia (P ≤ 0.001), kidney damage as indicated by an increase in creatinine concentration (P ≤ 0.001) and urea level (P ≤ 0.01). In the course of the research, a slight decrease in indicators of the main erythrocyte indices was established, namely, a decrease in MCHC (P ≤ 0.05), which indicates the development of anemia. As a result of an experimental study of the morphological parameters of blood, a complex pathogenesis of the disease with panleukopenia with multiple organ failure and the development of pathological immunosuppression and hepato-pancreatic-renal syndrome was established.

Abstract 8904: Predictors of Type 1 and Type 2 Myocardial Infarction

Background: Our understanding of the risk factors for myocardial infarction (MI) is limited to type 1 events caused by atherosclerotic plaque rupture and thrombosis. It is unclear whether similar risk factors are related to future type 2 MI events. Hypothesis: Cardiovascular disease risk factors are important in predicting type 2 MI Method: We conducted a secondary analysis of a multi-centre randomised trial population of 48,282 consecutive patients attending hospital with suspected acute coronary syndrome. Cox regression was used to identify predictors of future type 1 and type 2 MI during a one-year follow-up period. Results: During one-year of follow up 1,331 patients had a subsequent MI, with 924 and 407 adjudicated as type 1 and type 2 myocardial infarction, respectively. Risk factors for type 1 and type 2 MI were similar, with age, hyperlipidaemia, diabetes, increasing creatinine concentration, and known coronary disease important predictors for both (P&lt;0.05 for all). Whilst male sex is an established risk factor for type 1 MI, sex did not predict type 2 myocardial infarction (adjusted HR 0.82, 95% CI 0.66 to 1.01). The strongest predictor of type 2 MI was a prior history of type 2 events (aHR 6.18, 95% CI 4.70 to 8.12, Figure). Conclusions: Cardiovascular risk factors associated with type 1 MI are also important predictors of type 2 MI, suggesting common mechanisms including coronary disease are important. Treatment of these risk factors may reduce future susceptibility to both type 1 and type 2 MI.

Evaluation of the systemic alterations triggers by Porthidium lansbergii lansbergii snake venom

The systemic effects generated by Porthidium lansbergii lansbergii envenoming, a species found in the northern region of Colombia, is poorly known. The present study aimed to analyze for the first time the mice's behavior, the histological alterations, and changes in biochemical markers levels resulting from the intraperitoneal injection of an LD50 of P. lansbergii lansbergii snake venom on mice. The envenoming mice displayed hypodynamic condition, clonic head movements, accompanied by bradypnea and thoracoabdominal imbalance. After 7 h of envenoming, the mice showed an ecchymotic region at the injection site, including bleeding in the pleural, liver, and kidney capsules. The effect on the brain revealed a micro-hemorrhage in the sensorimotor cortex with substantial loss of neurons. The venom caused dilated blood vessels in lung tissue, with endothelial necrosis associated with alveolar rupture. The liver showed parenchyma alteration with many extravasated erythrocytes. The kidneys exhibited renal tubules necrosis and a statistically significant increase in creatinine concentration. ALP and ALT's enzymatic activities remained constant at 7 h after envenoming but increased at 12 h. AST and LDH were significantly increased at 7 h but decreased to the near baseline 12 h after venom administration. Massive hemorrhages could trigger a hypovolemic shock, which could lead to death after several h without treatment. Knowledge of P. lansbergii lansbergii snake bites' injuries is essential to make the appropriate diagnostic in human envenoming cases by this snake.

MODERN VIEW ON DIAGNOSTICS AND TREATMENT OF ACUTE KIDNEY INJURY IN FULL-TERM NEWBORNS WITH HYPOXIC-ISCHEMIC ENCEPHALOPATHY

Objective: to analyze the scientific literature sources inorder to study and systematize data on modern aspects ofdiagnosis and treatment of acute kidney injury in full-termnewborns with hypoxic-ischemic encephalopathy.Perinatal asphyxia is a multisystem disorder resultingfrom redistribution of blood flow, with renal dysfunctionbeing one of the most common complications and reaching50% of other multi-organ lesions. Acute kidney injuryis a complicated multifocal syndrome manifested by aclinical decrease of the diuresis rate and characterized bya rapid increase of creatinine concentration in laboratoryconditions. High serum creatinine level is not a specificsign of kidney injury, its level changes much later thanglomerular filtration rate, and depends on many nonrenal factors. Furosemide is the only direct diuretic inchildren, but its use is limited in this pathology. Thereare no recommendations on the frequency and duration offurosemide administration.Conclusions: The study of new diagnostic markersand possibilities of using drugs that increase glomerularfiltration in the kidneys in comparison with furosemide, andpreventing the progression of their damage, determiningthe minimal preventive dose, terms and frequency ofadministration, the study of early and late consequences,side effects of this therapy, is a promising direction ofscientific search.