Increased Body Weight Research Articles

Occurrence of sleep disorders and the value of body mass index of seniors and the quality of their aging.

The aim of the study was to assess the occurrence of sleep disorders and the value of body mass index in seniors, taking into account their quality of aging and participation in classes at Universities of the Third Age (UTAs). The study involved 631 (100%) seniors from the Silesian Voivodeship, aged 60 to 96 years (𝑥̅ = 70.28 ± 6.09 years). An original survey questionnaire was used, supplemented by the Successful Aging Scale and the Athens Insomnia Scale questionnaires. The median of successful aging index (SAI) points obtained by UTAs "+" seniors was 11.71, while the median of SAI points obtained by UTAs "-" seniors was 11.18. Sleep disorders were more common in women than in men as well as among people aged 71-80 years compared to other groups. There were statistically significant weak negative correlations between the points obtained in Successful Aging Scale questionaire domains and the number of points in the Athens Insomnia Scale as well as body mass index value. The occurrence of sleep disorders and abnormally increased body weight were associated with a deterioration in the quality of aging in the studied seniors. Seniors participating in UTAs activities had a better quality of aging than people who denied participating in the mentioned activity. Actions should be constantly taken to improve the quality of the aging process of seniors, including providing them with access to contacts with other people, treating sleep disorders and educating them on the benefits of a healthy lifestyle and maintaining a proper body weight. Geriatr Gerontol Int 2025; ••: ••-••.

Mizagliflozin ameliorates diabetes induced kidney injury by inhibitor inhibit inflammation and oxidative stress.

Mizagliflozin (MIZ) is a specific inhibitor of sodium-glucose cotransport protein 1 (SGLT1) originally developed as a medication for diabetes. To explore the impact of MIZ on diabetic nephropathy (DN). Diabetic mice were created using db/db mice. They were administered either a low dose (0.5 mg/kg) or a high dose (1.0 mg/kg) of the SGLT1 inhibitor MIZ via stomach gavage for 8 weeks. Subsequently, mesangial cells (MCs) were isolated and subjected to high glucose conditions in culture to assess the effects of MIZ on DN. The results showed that low doses of MIZ significantly reduced albuminuria to a level comparable to that achieved with high doses in db/db mice. High doses of MIZ led to a substantial increase in body weight in mice, along with decreased blood glucose levels and food intake. Moreover, the intervention with high-dose MIZ notably decreased the expression of extracellular matrix genes, such as collagen type 1 alpha 1 mRNA levels. While the expression of SGLT1 increased after exposure to high glucose, it decreased following treatment with MIZ. Furthermore, MIZ intervention was more effective in improving lactate dehydrogenase levels in MCs induced by high glucose compared to canagliflozin. MIZ also significantly elevated levels of antioxidant enzymes superoxide dismutase, catalase, and glutathione, while reducing malondialdehyde levels. These findings indicate that MIZ can ameliorate DN by inhibiting SGLT1, inflammation, and oxidative stress.

EFFICACY AND SAFETY OF DOLUTEGRAVIR/LAMIVUDINE IN ANTIRETROVIRAL THERAPY-NAIVE PEOPLE LIVING WITH HIV-1 AND WITH HIGH-LEVEL VIREMIA.

Dual regimen dolutegravir/lamivudine (DOL/3TC) showed potent efficacy and favourable safety in both antiretroviral therapy-naïve and -experienced patients, but data from real life about naive people with high-level viremia are still lacking. We performed a retrospective cohort study of people living with HIV (PLWH) who were naive to antiretroviral therapy, had baseline HIV-1 RNA ranging from 100000 to 500000 copies/mL, and initated DOL/3TC. Virological efficacy and changes in immunological parameters after 12 months of treatment were evaluated and compared with highly viremic PLWH who started a triple antiretroviral combination. Inclusion criteria were met by 58 patients with median age of 43.4 years. At baseline, mean HIV RNA was 5.4 log10 and mean CD4 T lymphocyte count was 488 cells/mm3. HIV RNA <50 copies/mL was obtained in 45 patients (77.6% in the intention-to-treat analysis) after 6 months and in 53 (91.4%) after 12 months. Reasons for treatment failure were virological failure in two cases and adverse events in three. No significant changes in median value of lipids were reported, while there was a not significant increase in body weight (+1.18 Kg). Virological and immunological response at month 12 in patients on DOL/3TC was comparable to that observed in 50 naive patients with high-level viremia and starting a triple antiretroviral therapy. In this real-life cohort of naive patients with high-level viremia, DOL/3TC was associated with high virological efficacy and good tolerability after 12 months, supporting use of this dual regimen also in persons with high initial viremia.

Targeting EP2 Receptor Improves Muscle and Bone Health in Dystrophin−/−/Utrophin−/− Double-Knockout Mice

Duchenne muscular dystrophy (DMD) is a severe genetic muscle disease occurring due to mutations of the dystrophin gene. There is no cure for DMD. Using a dystrophin−/−utrophin−/− (DKO-Hom) mouse model, we investigated the PGE2/EP2 pathway in the pathogenesis of dystrophic muscle and its potential as a therapeutic target. We found that Ep2, Ep4, Cox-2, 15-Pgdh mRNA, and PGE2 were significantly increased in DKO-Hom mice compared to wild-type (WT) mice. The EP2 and EP4 receptors were mainly expressed in CD68+ macrophages and were significantly increased in the muscle tissues of both dystrophin−/− (mdx) and DKO-Hom mice compared to WT mice. Osteogenic and osteoclastogenic gene expression in skeletal muscle also increased in DKO-Hom mice, which correlates with severe muscle heterotopic ossification (HO). Treatment of DKO-Hom mice with the EP2 antagonist PF04418948 for 2 weeks increased body weight and reduced HO and muscle pathology by decreasing both total macrophages (CD68+) and senescent macrophages (CD68+P21+), while increasing endothelial cells (CD31+). PF04418948 also increased bone volume/total volume (BV/TV), the trabecular thickness (Tb.Th) of the tibia trabecular bone, and the cortical bone thickness of both the femur and tibia without affecting spine trabecular bone microarchitecture. In summary, our results indicate that targeting EP2 improves muscle pathology and improves bone mass in DKO mice.

Aqueous Leaf Extract of Azadirachta indica Protects Against Gentamicin-Induced Kidney Injury via Decreases in Renal Function, Inflammation, and Apoptosis Markers.

The effect of the aqueous extract of Azadirachta indica (AAI) on gentamicin (GEN)-induced kidney injury was investigated. The study involves 20 adult male Wistar rats (housed in four separate plastic cages) such that graded dosages of AAI were administered to the experimental group for 14 days per oral (PO) before exposure to GEN toxicity (100 mg/kg) for 1 week. At the end of the study, comparisons of some markers of renal functions, antioxidant status, and inflammatory and apoptotic markers were made between the control, GEN, and AAI-pretreated groups at P < .05. The result showed that GEN treatment caused a significant increase (P < .05) in body weight, kidney weight, urea, bilirubin, kidney injury molecule 1 (KIM 1), cystatin C, malondialdehyde (MDA), reduced glutathione (GSH), tumor necrotic factor alpha (TNF-α), interleukin-1 (IL-2), caspase-3, and B-cell lymphoma-2 associated X (BAX) as well as a significant decrease (P < .05) in superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (Gpx), and B-cell lymphoma (BCL)-2 level. Pre-treatment with graded doses of AAI caused a significant increase in urea, CAT, and GPx as well as a significant decrease (P < .05) in kidney weight, bilirubin, KIM 1, cystatin C, MDA, GSH, SOD, TNF-α, IL-2, caspase-3, BAX, and BCL-2. There was an appreciable difference in the kidney histology of the AAI pre-treated groups compared with the GEN. Hence, the extract has prophylactic potential in managing GEN-induced nephrotoxicity by decreasing the markers of renal function and inflammation and downregulating the markers of apoptosis.

THE INCREASE IN BODY WEIGHT, SMALL INTESTINE LENGTH, AND FEED CONVERSION RATIO (FCR) OF BROILER CHICKENS GIVEN HEAT STRESS AND BANANA BLOSSOM PEEL EXTRACT (Musa paradisiaca)

This study aims to determine the increased of body weight, small intestine length, and food conversion ratio (FCR) of broiler chickens given heat stress and banana blossom peel extract (Musa paradisiaca). This study was an experimental study using a completely randomized design (CRD) consisting of five treatments. The experimental animals used in this study were female broiler chickens of the Cobb strain, 2 weeks old and weighing between 350-400 g, totaling 25 chickens. Chickens in P0 were not exposed to heat stress and banana blossom peel extract, while the chicken in P1, P2, P3, and P4 were exposed to heat stress and administered with 0.0, 500, 1000, 1500 mg/L banana blossom peel extract, respectively. The temperature in the heated cage accumulated slowly starting at 10:00 WIB and was maintained stable at 33±1° C for five hours. Banana blossom peel extract was given by dissolving it in drinking water for two hours (at 10:00) before the temperature in the cage reached 33±1° C. The data obtained were processed using the Kruskal-Wallis and continued with the Mann-Whitney Test. The results showed that there was a significant difference between treatments P1 and P4 on body weight gain and small intestine length (P0.05). Meanwhile, for the FCR value, only the P4 group met the standard, which was 1.57. Giving banana blossom peel extract at a dose of 1500 mg/L has been able to increase body weight, small intestine length, and decrease the FCR value in broiler chickens experiencing heat stress with a temperatu re of 33±1° C for 5 hours with a duration of 20 days.

Evaluation of the hypoglycemic, hypolipidemic and antioxidant activities of Phoenix dactylifera l. Date seeds extract in high fructose diet-induced diabetic rats

Diabetes is a growing global epidemic, necessitating effective management to prevent long-term complications. Natural substances are increasingly explored as alternatives to synthetic drugs due to their affordability and lower risk of side effects. This study investigates the qualitative composition and the hypoglycemic, hypolipidemic, and antioxidant activities of date seed extract (Phoenix dactylifera L.) in diabetic rats fed a high-fructose diet. Male rats were divided into four groups (n=6): Control (C, standard diet), Fructose Diabetic (FD, 60% fructose diet), and Control and Diabetic groups receiving daily 30 mL of aqueous date seed extract (C-aqDS, FD-aqDS). Weight gain, food and energy intake were monitored, while blood samples were analyzed for biochemical and oxidant/antioxidant markers. High-fructose consumption significantly increased body weight, energy intake, and apparent nutritional efficiency, while food intake remained unchanged. Biochemical markers, including glucose, glycated hemoglobin, uric acid, urea, creatinine, lipids, and liver enzymes, were elevated in FD rats compared to controls. Oxidative stress markers (MDA, PC) were increased, and antioxidant markers (catalase, SOD, GSH) decreased. Treatment with date seed extract significantly mitigated these abnormalities, reducing weight, biochemical parameters, and oxidative stress markers while enhancing antioxidant defenses. This study highlights the richness of date seed aqueous extract in bioactive compounds and its ability to alleviate early diabetic complications and oxidative stress in high-fructose-induced diabetic rats. These findings suggest its potential as a functional food for diabetes management.

Chronic low-dose REV-ERBs agonist SR9009 mitigates constant light-induced weight gain and insulin resistance via adipogenesis modulation.

Obesity and circadian rhythm disruption are significant global health concerns, contributing to an increased risk of metabolic disorders. Both adipose tissue and circadian rhythms play critical roles in maintaining energy homeostasis, and their dysfunction is closely linked to obesity. This study aimed to assess the effects of chronic low-dose SR9009, a REV-ERB ligand, on circadian disruption induced by constant light exposure in mice. Mice were exposed to constant light for eight weeks (LL mice), resulting in increased body weight, insulin resistance, white fat mass, and altered circadian clock gene expression. Low-dose SR9009 (10 mg/kg daily) was administered chronically to assess its impact on these metabolic disruptions. LL mice treated with SR9009 for eight weeks showed reduced weight gain, insulin resistance, and white fat mass but no significant impact on overall energy homeostasis. SR9009 suppressed Bmal1 expression and restored Rev-erbα and Rev-erbβ expression in white and brown adipose tissue (WAT and BAT). In vitro studies using 3T3-L1 cells indicated that SR9009 inhibited adipogenesis, leading to further investigation in vivo. SR9009 restored ChREBP1a and Srebp-1c expression in BAT but did not affect inflammatory cytokine or adipokine gene expression, nor did it restore Fasn, Pparγ, and Prom1 expression in both WAT and BAT. These findings suggest that SR9009 may be a potential therapeutic for preventing weight gain and insulin resistance caused by circadian disruptions, likely through adipogenesis inhibition, though its effects on other metabolic pathways remain limited at low doses.

Journal Review: Effectiveness of Supplementary Feed Ingredients on Egg Productivity and Body Weight Gain of Balibangtan Superior Hens

Feed is a major determining factor in the success of layer and broiler farming. The nutritional content of the feed must meet the requirements to increase livestock productivity. To improve chicken performance, we use additional feed. This review aims to determine the effectiveness of feed ingredients as a feed supplementation for chickens by using production waste, forage, household waste, forage, and enzymes to increase the growth and production of KUB chickens. Ingredients that can be used as feed additives include coconut pulp, pumpkin waste flour, papaya leaf waste, teak leaf extract, moringa leaves, tilapia plants, betel leaf juice, BS4, selenium chitosan, phytase, xylooligosaccharide, kesum leaf flour, sago pulp, spinach waste, and BSF maggot. The results of this review showed that the most effective feed ingredient was the supplementation of teak leaf extract (Tectona grandis), with a protein content of 4.9%, being the most effective feed ingredient in increasing egg productivity up to 15%. The supplementation of kesum leaf flour, with an average body weight gain of 426.97 g/head, is the most effective feed ingredient for increasing body weight growth in KUB chickens.

Alchornea cordifolia leaf-extract ameliorates bisphenol A-induced obesity in male Wistar rats

BackgroundBisphenol-A (BPA), a chemical impregnated into plastic bottles which has been linked to obesity and its associated health problems, was used to induce obesity in this study. The cost and reported limitations and side effects of orthodox medications employed for weight management, have necessitated a shift in attention to medicinal plants, given their comparative advantage and the common belief amongst locals that plants are free from negative side effects. One plant that has found application for weight loss amongst the local population in Nigeria is Alchornea cordifolia which commonly grows in Africa and has been confirmed to possess numerous medicinal properties including anti-inflammatory, anti-diarrheal, hepaprotective, antiviral, and anti-diabetic effects.ObjectiveTo evaluate the obesity attenuation effect of Alchornea cordifolia ethanol extract (ACEE) and other associated macrovascular health risks in BPA-induced obesity rat model.MethodsThirty (30) young male Wistar rats (80–100 g) divided into five groups of six rats each, were administered BPA (50 mg/kg), once a day, p.o. for four weeks in order to induce obesity. Afterwards, obesity was confirmed using anthropometric indicators, and the animals were treated for another four weeks as follows: Group 1 (Normal control) received drug/extract reconstitution solvent, olive oil, Group 2 (obese control) received 50 mg/kg of BPA, Group 3 received a conventional anti-obesity drug, Orlistat (30 mg/kg), Group 4 received ACEE (500 mg/kg), and Group 5 received ACEE (1000 mg/kg). At the end of study, anthropometric indicators and relevant serum biochemical indicators were determined using standard procedures.ResultsThe results obtained showed that oral BPA administration caused obesity i.e. increased body weight, Lee’s Index and waist circumference, compared to the normal control (p < 0.05). Moreover, BPA treatment was found to alter measured serum biochemical indicators, (FBG, lipid profile, liver and kidney function indicators) and the lipid regulating enzymes (pancreatic lipase and HMG-CoA reductase activities), relative to the normal control (p < 0.05). However, upon 4-week ACEE oral treatment at 500 and 1000 mg/kg b.w., there was observed significant attenuation of the anthropometric indices, serum biochemical indices and lipid metabolizing enzymes studied (p < 0.05).ConclusionTogether, the results obtained from this study have scientifically validated the traditional use of Alchornea cordifolia leaves in body weight control, hence suggest that the leaves may possess natural products useful in the management of obesity and its related complications.

Growth performance, carcass traits, meat composition, digestibility coefficients and caecal microbiota of growing rabbits fed diets supplemented with a herbal mixture.

The study investigates the effect of dietary herbal mixture (HM) levels on growing New Zealand White (NZW) rabbits' performance, carcass characteristics, blood biochemicals, and microbiological characteristics from 5 to 13 weeks of age. In this study, 96 New Zealand White rabbits (male and female ratio 1:1) were used, and they were five weeks old. The rabbits were at random allocated into four experimental groups (n = 24 each) comprising 12 replicates, each with two rabbits. The 1st group acted as the control and received feed without additional supplements. The 2nd group received 0.30% HM, the 3rd group received 0.50% HM, and the 4th group received 0.70% HM. As the concentration of the herbal mixture extract was raised, the inhibitory zones grew significantly. The addition of HM to rabbit diets at a 0.5% concentration significantly increased body weight and weight gain compared to the control diet and other treatments. The apparent digestibility coefficient of ether extracts and the rabbits' capacity to absorb lipids significantly increased with increasing HM levels up to 0.7%. In addition, the HM increased high-density lipoprotein (HDL) and decreased triglycerides and low-density lipoprotein (LDL) in the blood. Interestingly, when rabbits were fed varying amounts of HM, all types of bacteria significantly declined, yet helpful lactic acid bacteria increased. In conclusion, HM supplementation improved rabbit growth, blood parameters, and gut microbial balance, with a 0.5% level yielding the best results, indicating that HM has significant potential as a novel feed supplement for growing rabbits.

Astrocyte Loss Augments Body Weight Through Reduction in Adipose Sympathetic Outflows.

Emerging evidence indicates that astrocytes modulate energy metabolism and homeostasis. However, one important but poorly understood element is the necessity of astrocytes in the control of body weight. Here, we apply viral vector-assisted brain-region selective loss of astrocytes to define physiological roles played by astrocytes in the arcuate nucleus of the hypothalamus (ARH) and to elucidate the involved mechanism. We find that astrocyte loss potently augments body weight in adult mice fed chow or high-fat diet. Mechanistically, we find that the loss of astrocytes reduces adipose tissue norepinephrine (NE) contents and chemogenetic stimulation of adipose tissue sympathetic inputs reverses the astrocyte loss-induced increase in body weight. Collectively, our findings in this study suggest a crucial physiological role of astrocytes in preventing diet-induced energy surfeit and obesity by modulating adipose tissue lipid metabolism through central sympathetic outflows to adipose tissues.

Chronic artificial light exposure in daytime and reversed light: Dark cycle inhibit anti-apoptotic cytokines and defect Bcl-2 in peripheral lymphoid tissues during acute systemic inflammatory response to lipopolysaccharide.

The disturbed light: dark (LD) cycle has been associated with critical complications, including obesity, diabetes and cancer. In the present study, we investigated the chronic effects of artificial light at daytime (AL) and light at night (RAL) after intraperitoneal (i.p.) injection of saline and 0.5mg/kg lipopolysaccharide (LPS) in male Wistar rats. Liver and kidney parameters, fasting blood glucose (FBG), melatonin level, immunohistochemical examinations of B-cell lymphoma-2 (Bcl-2) in spleen and mesenteric lymph and serum antiapoptotic cytokines [interleukin (IL-) 2, 7 and 1]. After 16weeks of a daily disturbed LD cycle, RAL increased body weight, upgraded FBG and altered liver and kidney functions with surprisingly increased daytime plasma melatonin. AL+LPS and RAL+LPS rats suffered significantly higher oxidative-nitrosative stress compared to NL+LPS. Oxidative-nitrosative stress was associated with multi-organ inflammation in hepatic, renal, pancreatic, splenic and mesenteric lymph node tissues due to LPS-induced endotoxemia. Anti-apoptotic Bcl-2 activity in peripheral lymphoid organs (spleen and mesenteric lymph node) was lowered due to AL and RAL regimens. At the same pattern, lowering of antiapoptotic serum levels of IL-2, IL-7 and IL-15 indicate alteration of cell cycle and the shifted ability of cells to undergo apoptosis due to abnormal light pollution. Here, the increased lymphocyte apoptosis in lymphoid tissues due to disturbed LD cycle defects the host defense, dysregulates the inflammatory immune response and dysregulates the immune tolerance during acute systemic inflammation due to LPS.

Dietary supplementation with JASMINUM SAMBAC leaf powder: effects on GROWTH, HEMATOLOGY, and IMMUNE PARAMETERS in BROILER CHICKENS

Food production needs to expand by 70% to keep up with the expected considerable increase in the world population by 2050. As a major source of high-quality protein, poultry products are essential to the world's food supply. Nonetheless, problems with industrial mechanization, pollution, animal welfare, and antibiotic resistance affect the poultry industry. The objective of this study was to assess the impact of adding Jasminum sambac leaf powder (JSLP) to the feed of broiler chickens on their growth performance, coliform bacterial levels, and immunological function. During a 42-day period, 100 day-old Ross broiler chicks were randomly allocated to one of four treatment groups, each consisting of 25 replicates: T0 (control) with no additives, T1 supplemented with 3% JSLP, T2 with 6% JSLP, and T3 with 9% JSLP. One-way ANOVA was used to determine statistical inference among groups. Feeding JSLP resulted in a significant increase in body weight (BW), body weight gain (BWG), and feed intake (P<0.05), while faecal coliforms were significantly reduced (P<0.01). Further, haemoglobin concentration and red blood cell counts (RBC) on days 14, 28, and 42, were significantly increased(P<0.01). Additionally, significant differences in differential leucocyte counts (DLC) were observed between the JSLP-treated groups (P<0.001). Overall, JSLP supplementation positively influenced broiler chicken growth indices and haematological profiles, though its impact on immunological parameters warrants further investigation.

Effects of Amino Acid Supplementation on the Growth Performance of Broiler Chicks Challenged With Coccidiosis: A Meta-Analysis.

This meta-analysis aimed to evaluate the effects of amino acid supplementation on the growth performance of broiler chicks challenged with coccidiosis. Data were collected on authorship, publication year, study sample characteristics and outcomes of interest related to growth performance. Effect sizes were calculated for both overall effects and the individual effects of each amino acid. The effects of moderator factors, such as supplementation levels and duration, were also assessed, and bias was calculated. Works were obtained from PubMed, Elsevier, ScienceDirect, Wiley, Springer Link and Scopus databases, with the help of search engines like Google Scholar and ResearchGate, covering the period from 2013 to 2023. A random-effects model was used. Thirteen works were included in the current analysis, examining the effects of arginine (n=4), methionine (n=6), threonine (n=3) and glutamine (n=3). Amino acid supplementation [0.326, CI 95% (0.297-0.355); p=0.000], arginine [0.430, CI 95% (0.293-0.568); p=0.000] and threonine [0.793, CI 95% (0.193-1.38); p=0.009] significantly increased body weight. Amino acid supplementation [0.052, CI 95% (0.022-0.081); p=0.000] and arginine [0.317, CI 95% (0.049-0.585); p=0.000] also significantly increased feed intake. Higher levels of amino acids (≥1.50%) and longer supplementation periods (≥15 days) significantly increased body weight and feed intake, while reducing the feed conversion ratio. Funnel plots, fail-safe N and Egger tests showed no evidence of bias. In conclusion, arginine supplementation has the potential to increase body weight and feed intake in poultry challenged with coccidiosis. Higher doses of amino acids and longer supplementation periods are recommended in such cases.

Protective role of 3-mercaptopyruvate sulfurtransferase (MPST) in the development of metabolic syndrome and vascular inflammation.

Metabolic syndrome (MetS) is a cluster of metabolic abnormalities that occur concurrently and increase the risk of cardiovascular disease. 3-mercaptopyruvate sulfurtransferase (MPST) is a cysteine-catabolizing enzyme that yields pyruvate and hydrogen sulfide (H2S) and plays a central role in the regulation of energy homeostasis. Herein, we seek to investigate the role of MPST/H2S in MetS and its cardiovascular consequences using a mouse model of the disease. Mice were fed a high-fat diet (HFD) for 15 weeks to induce obesity and hyperglycemia and administrated a nitric oxide synthase inhibitor, during the last 5 weeks to induce hypertension and MetS. This model caused a mild left ventricular (LV) diastolic dysfunction and vascular endothelial dysfunction. Free H2S and sulfane-sulfur levels were decreased in the aorta, but unaltered in the heart. Also, downregulation of MPST and thiosulfate sulfuretransferase (TST) were observed in the aorta. Global deletion of Mpst (Mpst-/-) resulted in increased body weight and greater glucose intolerance in mice with MetS, without affecting their blood pressure, and caused an upregulation of genes involved in immune responses in the vasculature suggestive of T-cell infiltration and activation. Pharmacological restoration of H2S levels ameliorated the comorbidities of MetS; GYY4137 administration reduced body weight and blood pressure, attenuated cardiac fibrosis and improved glucose handling and endothelium-dependent relaxation. In conclusion, this study found that reduced MPST/H2S exacerbates the pathological changes associated with MetS and contributes to vascular inflammation. H2S supplementation emerges as a potential therapeutic approach to treat the abnormalities associated with MetS.

Hesperidin enhances broiler growth performance by augmenting gastric acid secretion via the proton pump pathway.

Hesperidin exhibits promising potential as a feed additive for augmenting gastric acid secretion in animals. Gastrointestinal function is essential for animal growth and the efficient digestion of dietary nutrients, with gastric acid secretion serving as one of its critical components. The secretion of gastric acid, together with other digestive fluids and substances, significantly influences the digestion and absorption of animal feed, which in turn affects growth performance. However, there is limited research regarding the application of hesperidin as a feed additive to enhance gastric acid secretion. The present study aims to evaluate the efficacy of hesperidin as a feed additive in enhancing gastric acid secretion and to elucidate its underlying mechanisms. A total of 200 newly hatched (1-day-old) broilers with similar body weight were randomly allocated into four groups as follows: the control group receiving only the basal diet, and the other three groups supplemented with 50, 100, and 150 mg of hesperidin per kg of the basal diet, respectively. Each group consisted of five replicates with ten broilers per replicate, and the feeding trial lasted for a duration of 21 days. The growth performance was evaluated by monitoring feed intake and body weight throughout the trial. A four-day nutrient utilization trial was conducted prior to the conclusion of the feeding experiment. Adoption of the total collection method, the collected droppings were weighed and dried at 65 °C. Fifteen broilers from each group were euthanized and immediately dissected to obtain gizzard, proventriculus, gizzard chyme, and jugular blood samples, The proventriculus and gizzard weight were weighed and the pH of gizzard chyme was measured at the same time. The collected jugular venous blood was used to assess gastrin levels, whereas chicken gizzard chyme was utilized for the analysis of lactate, hydrochloric acid, and pepsin activity. Proventriculus and gizzard tissues were used to evaluate pepsinogen levels, perform hematoxylin-eosin (H&E) staining, conduct enzyme-linked immunosorbent assays (ELISAs) for key proton pump components, and assess proton pump activity. The results demonstrated that, in comparison to the control group, both the 100mg/Kg and 150 mg/Kg groups exhibited a significant increase in final body weight (FBW) and average daily gain (ADG) (P < 0.05). Additionally, the feed to gain ratio (F/G) was significantly reduced in the 150mg/Kg group (P < 0.05). The results of the nutrient utilization trial indicate that all treatment groups had significantly higher levels of dry matter (DM) and ether extract (EE) compared to the control group (P < 0.05). Furthermore, crude protein (CP) and gross energy (ME) were significantly higher in the 100mg/Kg and 150mg/Kg groups than in both the control group and the 50mg/Kg group (P < 0.05). The inclusion of hesperidin in broiler diets leads to significant improvements in stomach development and lactic acid content, while pH and hydrochloric acid content exhibit opposite trends (P < 0.05). Supplementation of broiler diets with hesperidin at doses of 100 mg/Kg and 150 mg/Kg significantly up-regulates pepsin activity and pepsinogen levels (P < 0.05). Incorporation of hesperidin into the broilers' diet significantly enhances parietal cell numbers (P < 0.05). Dietary supplementation of hesperidin in broilers effectively up-regulates key signaling pathways and intracellular signal substances involved in proton pump activation (P < 0.05). The proton pump activity also exhibited a significant increase compared to the control group of 100mg/Kg and 150mg/Kg (P < 0.05) in our findings. In conclusion, hesperidin exhibits promising potential as a feed additive for broilers, and it can improve the growth performance of broilers by increasing gastric acid secretion and promoting nutrient utilization through activation of proton pump. Notably, basal diet supplemented with 150mg/Kg hesperidin demonstrates superior efficacy.

Olanzapine exposure disordered lipid metabolism, gut microbiota and behavior in zebrafish (Danio rerio).

Olanzapine (OLZ) is widely used in the treatment of schizophrenia, and its metabolic side effects have garnered significant attention in recent years. Despite this, the specific side effects of OLZ and the underlying mechanisms remain inadequately understood. To address this gap, zebrafish (Danio rerio) were exposed to OLZ at concentrations of 35.5, 177.5, and 355.5μg/L. The results indicated that exposure to OLZ significantly increased body weight, total cholesterol (TC), low-density lipoprotein (LDL), and triglycerides (TG). Histological analysis revealed notable lipid accumulation in the liver. Furthermore, lipid synthesis genes, including sterol regulatory element binding protein (srebp), acetyl CoA carboxylase (acc), and fatty acid synthesis gene (fas), were up-regulated. In contrast, genes related to lipid decomposition, such as lipoprotein lipase (lpl), hormone-sensitive triglyceride lipase (hsl), and carnitine palmitoyltransferase 1b (cpt1b), were down-regulated. Subsequent analysis of zebrafish behavior showed reduced motor activity, sociability, and anxiety-like behavior in OLZ-exposed zebrafish, consistent with the results of neurotransmitter related gene expression. Following OLZ treatment, the expression of tryptophan hydroxylase (tph), tyrosine hydroxylase (th), dopamine transporter (dat), glutaminase (glsa), and glutamic acid decarboxylase 1b (gad1b) was upregulated. Additionally, the diversity of intestinal flora decreased after OLZ exposure, and the structure of the intestinal microbiota changed significantly compared to the control group. At the genus level, the abundance of Plesiomonas was upregulated, while the abundances of Bacillus and Cetobacterium were downregulated in the OLZ-exposed group. Furthermore, the results of the correlation analysis indicated that lipid metabolism and behavioral changes were closely associated with the microbiota. This study clarified the side effects of OLZ, and also provided a basis for the reasonable discharge concentration of OLZ in water and clinical drug use.

Efficacy of anticoccidial drugs and their impacts on growth performance on caecal coccidiosis in broiler chicken

Coccidiosis is a fatal disease of poultry. In order to combat caecal coccidiosis of broiler, this study was designed to evaluate the comparative efficacy of sulfaclozine, toltrazuril and amprolium. 50 pcs of day old broiler chick were randomized and divided into five groups. The chicks of group T0 kept as non-infected, non-medicated control. The group Ti were kept as infected non treated and other three groups such as T1, T2, T3 were treated with sulfaclozine, toltrazuril and amprolium at a dose rate of 10 mg/kg body weight orally, respectively on day 21 and 24 of age. In chicks of group T1, sulfaclozine was given at rate of 10mg/kg body weight orally. Similarly chicks of group T2 were treated with toltrazuril 10 mg/kg and group T3 were treated with amprolium at the rate of 10 mg/kg body weight orally, respectively. After treatment, the efficacy, body weight, morbidity and mortality were studied. From the present research treatment there were sharp decline of OPG counts in all groups. But the decline was very significant (P&lt;0.05) in Group T3 which was 1.37±0.002 thousand. This study also suggested that the mean initial weight of chicks for all groups were almost similar, which was 37 g and above as recorded on day 1. The pre-infection body weight values at day 10 recorded for all groups were almost similar which was more than 217 g. Significant (P&lt;0.01) increase of body weight was recorded on day 21 following which the highest increase (780.21±1.29 g) was seen in Group T0 chicken (healthy control). After treatment (on day 24), there were significant increase in body weight of all groups used for therapeutic trial with the maximum mean weight gain in Group T3 (790.83±2.10g) which was the nearest value to Group T0 (healthy control). The clinical signs of caecal coccidiosis appeared on day 15 and became severe on day 21. A total of 6 chicks died during the experimental period from Group Ti (untreated affected group) and found 60% mortality. Only 3 birds died in Group T1 treated with sulfaclozine and 2 birds died in group T2 treated with toltrazuril and 2 birds died in group T3 group treated with amprolium.

Finding ways to correct nonspecific vaginitis in menopausal women with obesity

Objective — to increase the effectiveness of treatment of nonspecific vaginitis in menopausal women with excess body weight and obesity through long-term lifestyle modification. Materials and methods. 1178 patients aged 45—55 years were examined, including 317 with nonspecific vaginitis, who were divided into 3 study groups: with a healthy body weight, overweight and obesity. Each group was divided into three subgroups of comparison with standard therapy and three study groups that applied long-term lifestyle modification (actively and systematically used behavioral therapy, physical activity and a low-calorie diet) for 6 months. The control group consisted of 72 volunteers. Results and discussion. In the study subgroups of women who used long-term lifestyle modification, recurrences of nonspecific vaginitis were less frequent than in the corresponding comparison subgroups. Thus, after 6 months, in subgroups of women with a healthy body weight, the difference in the frequency of relapses after treatment was 40 % greater (34.2 vs 20.5 %), with excess body weight — 31.1 % (41.2 vs 28.4 %), with obesity — 29.8 % (55.1 vs 38.7 %). A significant deterioration in the state of the microbiota in menopausal patients with increased body weight and the effectiveness of the use of long-term lifestyle modification on its condition were established. It was proven that excess body weight and obesity in women negatively affected the frequency of nonspecific vaginitis, clinical picture, decreased quality of life, and frequency of relapses. Active and systematic use of behavioral therapy, physical activity, and a low-calorie diet in patients with nonspecific vaginitis in menopause had significant advantages in preventing relapses, compared to standard therapy, and in preserving and improving the quality of life of obese patients. Conclusions. Overweight and obese women are more likely than healthy women to suffer from nonspecific vaginitis, which is more severe and recurrent, accompanied by a decrease in quality of life and a disruption of the vaginal and skin biotope. After 6 months in obese postmenopausal women, the use of long-term lifestyle modification contributes to the restoration of the vaginal and skin microbiome, a decrease in the frequency of recurrences of nonspecific vaginitis and an improvement in quality of life compared with subgroups. with standard therapy. The best effect of treatment was observed in women with a healthy body weight, and the worst in the presence of obesity.