Incision Wound In Rats Research Articles

Mikania micrantha extract enhances cutaneous wound healing activity through the activation of FAK/Akt/mTOR cell signaling pathway

Mikania micrantha (MM) has been traditionally used for various health benefits, including mental health, anti-inflammatory, wound dressing, and healing of sores. However, the molecular mechanisms and dose required for the wound healing activity of MM have yet to be reported. Therefore, a study was conducted to evaluate the wound healing potential of a cold methanolic extract of MM through in vitro and in vivo studies. Human dermal fibroblast adult (HDFa) cells were treated with 0 (control), 75 ng/ml, 125 ng/ml, 250 ng/ml, and 500 ng/ml of MMmethanolic extract (MME) for 24 h. MME at 75 ng/ml has significantly (p˂0.05) promoted HDFa cell proliferation and migration. Further, MME has also been shown to enhance the invasiveness of human umbilical vascular endothelial cells (HUVECs), indicating the neovasculature for wound healing. The tube formation assay demonstrated a significant (p<0.05) increase in the angiogenic effect of the MME starting at a concentration of 75 ng/ml as compared to the control. Treatment of excision wounds in Wistar rats with 5% and 10% MME ointment significantly enhanced wound contraction compared to control animals. Incision wounds in rats treated with 5% and 10% MME showed a significant (p<0.01) increase in tensile strength compared to control. HDFa cells, and granulation tissue collected on day 14 post-wounding, revealed the modulation of the FAK/Akt/mTOR cell signaling pathway during the enhancement of wound healing. The results of gel zymography showed increased activity of MMP-2 and MMP-9 in the HDFa cells after treatment with the extract. It is concluded that MMEcan potentially accelerate cutaneous wound healing.

&lt;strong&gt;Effectiveness of natural lip balm cinnamon &lt;em&gt;(Cinnamomum burmannii)&lt;/em&gt; in accelerating the incision wound healing process in &lt;em&gt; rattus norvegicus &lt;/em&gt; &lt;/strong&gt;

ABSTRACT Introduction: Cinnamomum burmannii is commonly used in the treatment and is thought to be an anti-inflammation medicinal herb that may hasten recovery. Lip wound healing is a critical need for aesthetic performance. The purpose of this study is to evaluate the efficacy of natural lip balm C. Burmannii has been shown to speed up the healing of incision wounds in rats (Rattus norvegicus). Methods: This research was carried out in the laboratory biomedical Faculty of Medicine, Universitas Islam Bandung. In twenty-seven male rats weighing 200-300 g, a lip mucous incision of 10 mm length and 4 mm depth was surgically created. Simple random sampling was used to divide the animals into three treatment groups based on wound treatment after lip incision (The control group applied distillation water, the first group applied a lip balm placebo/base, and the second group applied natural lip balm C. burmannii) and then divided into three observation times (days 3rd, 7th, and14th). A lip biopsy was performed during each observation time to determine the success of the therapy. The macroscopic examination was carried out by counting the number of lip wounds that were closed around, while the microscopic examination was carried out by counting epithelialization with a Leica microscope and image raster software. Result: The length of the lip wound has greatly decreased in the C. Burmannii group with a p-value of 0,048 (p&lt;0.05, and epithelialization was comparable across the board. Conclusion: The use of Cinnamomum burmannii natural lip balm is effective in accelerating lip wound healing by bringing the lip wound closer together. Effectiveness of Natural lip balm Cinnamon (Cinnamomum burmannii) in accelerating the incision wound healing process in Rattus norvegicus.Keywords: natural lip balm cinnamomum burmannii; wound closer; epithelialization

Antibiotic-free combinational hyaluronic acid blend nanofibers for wound healing enhancement

An innovative approach in the functionalization of nanofibers (NFs) for wound healing relies on non-antibiotic combinational therapy to subdue microbial invasion while reducing antimicrobial resistance and enhancing healing. Despite great potentials, wound healing efficacy of NFs embedding antimicrobial metal nanoparticles (NPs)/essential oils has been scarcely documented. We developed combinational NFs using an electrospinnable hyaluronic acid/polyvinyl alcohol/polyethylene oxide blend embedding a new ZnO NPs/cinnamon essential oil (CEO) antimicrobial combination. Fourier transform infrared, X-ray diffraction and transmission electron microscopy confirmed the presence of HA and distribution of ZnO NPs and CEO within NFs. Results for mean diameter, thermal stability, hydrophilicity, tensile strength, in vitro biodegradability, and cytocompatibility of crosslinked combinational NFs were intermediate between those of their singly loaded counterparts. All NFs inhibited the growth of Staphylococcus aureus (S. aureus). Compared with singly loaded NFs, combinational NFs showed the greatest healing efficacy of full thickness S. aureus inoculated incision wounds in rats in terms of bacterial inhibition following a single application, healing speed, and quality of skin structure recovery as verified by morphological, microbiological, and histopathological studies. Results highlighted the potentials of metal NPs/essential oil functionalization of nanofibrous wound dressings as an emerging antibiotic-free combinational approach for more effective and safer wound healing.

Efektivitas Ekstrak Ikan Gabus (Channa striata) dan Ekstrak Kunyit (Curcuma domestica) terhadap Kesembuhan Luka Insisi pada Tikus Putih (Rattus norvegicus)

This study aimed to determine the effect of snakehead fish extracts (Channa striata) and turmeric extract (Curcuma domestica) towards incision wound healing in rats (Rattus norvegicus). Twenty-four male rats, aged 8-week-old, 200-300 grams of body weight divided into 4 groups: control (P0), treatment with povidone iodine as positive control (P1), treatment with snakehead fish extract (P2) and treatment with turmeric extract (P3). The rats were given premedication consisting of acepromazine 0.5 mg kg / BW and atropine 10 mg / kg BW. The rats were then anesthetized using ketamine 0.1 mg / kg BW. An incision was made on each rats’ back area along 2 cm with 0.05 mm depth and observed for 14 days. Observations were carried out every day with data collection at &lt;7 days, 7-14 days, and 14 days. The One-Way ANOVA statistical analysis resulted that the wound fluid parameters and the duration of wound healing showed no significant difference between P0, P1, P2 and P3. This study concluded that the administration of povidone iodine, snakehead fish extract, and turmeric extract influenced the healing of incision wounds in rats.

A Comparative Study of the Effect of Lignocaine and Tramadol on Healing of Infected wound in Wistar Rats

The study was conducted to assess the effect of lignocaine and tramadol on healing of Staphylococcus aureus infected incision wound in rats. Incision wounds were created in three groups consisting of six rats in each group. The rats were infected with Staphylococcus aureus inoculum and the incision was closed. The rats were treated according to their group – Group I (Normal saline), Group II (2% Lignocaine) and Group III (5% Tramadol). All the drugs were applied topically, once a day for 10 days. The parameters measured to evaluate the wound healing was breaking strength, Colony Forming Units/ mL (CFU/ mL) of the tissue and histopathological examination. Data analysis was by one-way (Analysis of Variance) ANOVA followed by post hoc Tukey’s test. A significant difference (p&lt;0.05 vs control) in the breaking strength and CFU/mL of tissue was observed between the control Group I and the test groups, Group II (2%lignocaine) and Group III (5% tramadol) when the drugs were used topically. Histopathological study did not show much difference in the morphology between the control and other groups, it revealed disorganised collagen fibres. This study suggests that lignocaine and tramadol do not impede healing of infected incision wound.

Novel microstructured sildenafil dosage forms as wound healing promoters

Purpose: Study the possible benefit of combining biodegradable polymers with sildenafil citrate (SC) in wound healing.Method: Biodegradable micronized powdered formulations of SC were prepared by spray drying using chitosan (P1) or chitosan/gum Arabic (P2). Powders were characterized by differential scanning calorimetry, Scanning electron microscope, particle size analysis, flow and swelling behavior. The powders were also incorporated into microstructured gels and in vitro SC release from powders and gels was tested. In vivo wound healing acceleration was tested by measuring area contraction of excision wounds and histologically. Post-healing tensile strength (TS) for incision wounds in rats receiving powder formulations was tested.Results: The powders were in the micron-size range showing no SC–polymers interaction. Powders had poor flow with angle of repose (θ) of 41 – 48°, and high moisture uptake reaching 107% for placebo powder Po1. Good excision wound healing was seen with P1 and G1 formulations showing 98.4 and 98.5% reduction in wound area, respectively, compared with 83% for the control. Incision wounds were improved with P1 showing TS value of 6.9 compared with 3.7 kg/cm2 for control. Histological examinations supported.Conclusion: Spray-dried chitosan/SC powder (P1) and its gel form (G1) could be promising wound healing promoters as supported by the histological examinations.

Topically applied Tetrapleura tetraptera stem-bark extract promotes healing of excision and incision wounds in rats.

Objective:The objective of the present study was to evaluate the in vivo wound healing effect of water extract of Tetrapleura tetraptera in stem-bark.Materials and Methods:The healing activity was studied in 40 male rats using excision and incision wounds on normal and dexamethasone-suppressed wound healing. For each model, rats were divided in four groups as follows: control, dexamethasone, T. tetraptera and dexamethasone combined with T. tetraptera. Results: Data recorded exhibited a significant effect by the extract in the epithelialization time within 14 and 18 days of the normal and dexamethasone-induced healing delay rats, respectively (P < 0.05). The extract also significantly increased the wound tensile strength in dexamethasone treated rats. Histological examination of incision wounds of the extract-treated group showed many fibroblasts and the same rats presented significant cutaneous tensile strength, suggesting important collagen crosslinkage.Conclusion:This study illustrated an excellent potential of the bark of T. tetraptera therapy on dermal wound healing with a possible mechanism of action related to epithelialization, contraction, and tensile strength improvement.

Biopolymeric nifedipine powder for acceleration of wound healing

Nine biodegradable polymeric powders of chitosan; with or without gelatin; containing nifedipine (NF), were prepared via spray drying for acceleration of wound healing. The angle of repose of powders ranged from 30° to 40° for F 3 and chitosan/gelatin (C 2), respectively. Upon spray drying, the mean particle size (PS) of chitosan was greatly reduced from 294 to 3.4 μm, while all formulae showed a PS near 3 μm. Specific surface area of the powders ranged from 0.06 × 10 5 to 2.03 × 10 5 cm 2 g. Powders exposure to glutraldehyde (GA) vapors gave smaller particles with higher densities with F 4 showing a mean PS of 0.4 μm. NF dissolution pattern in PBS, was highest from F 2 with 33% released after 30 min while F 3 showed only 4.5% NF released after the same period. Exposure to GA showed a reduction in NF release especially with F 5 showing 30% release after 3 h. Tensile strength of 12 days-post incision wound in rats showed a maximum value of 4.7 kg cm −2 for F 3 compared with 3.3 kg cm −2 for the control. Excision wounds treated with F 3 also showed a fully developed epithelium with normal keratinization, and an average wound contraction of 99% compared with 79.5% for the control.

Synthesis and Evaluation of Long-Acting D-Penicillamine Derivatives

This study extends the use of two lathyrogens, β-aminopropionitrile (BAPN) and D-penicillamine (DPA) from daily systemic or local-topical administration to long-time acting agents. This was achieved by converting the hydrophilic drugs into lipophilic derivatives. The synthesis of functional derivatives of DPA consisted in esterification with methyl-, hexyl-, or benzyl alcohols in the presence of thionylchloride. The esters formed were hydrochlorides, acidic and soluble in water. During neutralization in vitro or in vivo by tissue fluid, an oily substance is formed that elutes from a hydrogel polymer at a much slower rate than hydroplilic DPA itself. The degree of lipophilicity, measured as a partition coefficient between octanol/water, was highest for hexyl ester and lowest for methyl ester DPA. A single injection of either DPA hexyl ester HCl or 3-hexyl(amino) propionitrile into the full thickness skin incision wound in rats significantly lowered the breaking strength of the wound 12 days after injection, indicating the interference with collagen cross-linking. Both agents injected into the breast adenocarcinoma in Fisher rats significantly inhibited tumor growth without any signs of local or systemic toxicity. We conclude that these lipophilic lathyrogens with prolonged effectiveness are suitable in the treatment of pathologies, consisting of excessively cross-linked or deposited collagen (fibrotic adhesions, strictures, stenosis, and scar contractures) and in the treatment of single, solitary tumors, malignant and benign.

Three-dimensional hyaluronic acid grafts promote healing and reduce scar formation in skin incision wounds.

Hyaluronic acid (HA) has been found to play important roles in tissue regeneration and wound-healing processes. Fetal tissue with a high concentration of HA heals rapidly without scarring. The present study employed HA formed into three-dimensional strands with or without keratinocytes to treat full-thickness skin incision wounds in rats. Wound closure rates of HA strand grafts both with and without keratinocytes were substantially enhanced. The closure times of both HA grafts were less than 1 day (average 16 h), about 1/7 that of the contralateral control incisions (114 h, p <.01). Average wound areas after 10 days were HA-only graft: 0.151 mm2 +/- 0.035; HA + cell grafts: 0.143 mm2 +/- 0.036 and controls: 14.434 mm2 +/- 1.175, experimental areas were 1% of the controls (p < 0.01). Transforming growth factor (TGF) beta1 measured by immunostaining was remarkably reduced in HA-treated wounds compared to the controls. In conclusion, HA grafts appeared to produce a fetal-like environment with reduced TGF-beta1, which is known to be elevated in incipient scars. The HA strands with or without cultured cells may potentially improve clinical wound healing as well as reduce scar formation.

Collagen antibodies and their effect on wound healing

The influence of antibodies to collagen on wound healing was tested by the use of a standard incision wound in rats. The antisera--specific for the terminal determinants of the collagen molecule--were obtained from rabbits immunized with rat--or pig--skin collagen and injected intraperitoneally, i.e. systemically. Generalized immune reactions or signs of incompatibility were not observed. The injection of antisera on the 7th postoperative day led to a significant gain of tensile strength. This effect was achieved by the homologous antiserum (p less than 0.01) as well as by the heterologous antiserum (p less than 0.04). The heterologous antiserum further induced a significant fall of free serum hydroxyproline (p less than 0.005). The application of the heterologous antiserum on the 14th postoperative day caused a significant decrease of tensile strength (p less than 0.006). The results are referred to collagen-specific immune reactions and varying conditions in the wounded area during different phases of wound healing.