Some factors are experimentally investigated concerning the method of cavitation inception measurement. They are definition of cavitation inception, effect of air content in tested water, effect of methods of pressure change in the cavitation tunnel and its rate of change. The test body is a circular cylinder in the range of flow of subcritical Reynolds number. In this flow condition, the cavitation bubbles occur in the separated shear layer behind the cylinder with large scatter of incipient cavitation numbers, according to past investigations. One of the most effective definitions is proposed for cavitation inception in the present work, where the main results are obtained using the proposed definition : (1) the inception values can be greatly reduced under water with small air content of less than a critical value, (2) the inception point can be affected by pressure change rate under the condition of variable flow velocity. At the same time, the measurement uncertainty on cavitation inception is also shown through a large number of measurements in the present experiment.