Incidence Of Rot Research Articles

Impact of Nitrogen Fertilizer Rate and Timing on Short-day Onion Production

Onions (Allium cepa) are typically planted late fall and harvested in spring in the Vidalia, GA, USA, region. Onions grown here are renowned for their for sweetness and are marketed to consumers as Vidalia onions. High rainfall during the relatively long growing season (4 to 5 months) may result in nitrogen (N) leaching during production. Therefore, fertilizer applications are usually aligned with stages of crop development to ensure nutrient availability for the entire season. Although the impacts of N application rate have been previously investigated for Vidalia onion production, the optimal timing for the final N application of the season has not been determined. The objectives of this study were to determine the optimal timing of the last fertilizer N application (at bulb initiation, during bulb growth, or during bulb maturation) in conjunction with the impact of three N application rates (75, 105, and 135 lb/acre N) on yield and quality in Vidalia onion. Soil N levels were affected by N rate, year, and onion growth stage. In 2020, up to 135 lb/acre N was required to maximize onion yields, and in 2021, onion yields were unchanged among N fertilizer treatments. Final N applications at bulb initiation resulted in greater yields than applications made during bulb growth or bulb maturation. In addition, as the N rate increased and the time of final application occurred later in bulb development, pungency values increased. Incidence of sour skin (Burkholderia cepacia) and center rot (Pantoea sp.) diseases were greater in 2020 compared with 2021 and seemed to be affected by environmental conditions more than N fertilization.

Comparative incidence of Ganoderma basal stem rot and upper stem rot disease in oil palm in relation to soil type and geographical distribution

The greatest danger to oil palm sustainability in Indonesia is posed by basal stem rot (BSR) and upper stem rot (USR). However, information on their comparative incidence in relation to soil types and geo-distribution remains scarce. As such, data from annual surveys carried out over 95,000 ha of first-generation oil palms planted in Asian Agri estates was reviewed and analyzed. The highest infection levels occurred in North Sumatra, followed by Riau and Jambi. Likewise, infection levels were highest in peat (24.79 palms/ha), followed by alluvial (13.41 palms/ha), volcanic (7.25 palms/ha), and inland (0.07 palms/ha) soil types. Averaged over three provinces and all four soil types, infection due to USR was more dominant than BSR, comprising 52.3% and 47.7%, respectively. Molecular assays of infected tissue confirmed that both BSR and USR were caused by the genus Ganoderma with the major species being G. boninense. Longitudinal dissections of oil palm trunks infected by USR indicated no infection connectivity with the basal portion of the trunk, unlike BSR, where the infection is through root contact. Transmission through basidiospores is likely to play an essential role in USR epidemiology. Understanding pathogen distribution, species identity, and its transmission epidemiology are prerequisites to control diseases more effectively.

Enhancement of Strawberry Shelf Life via a Multisystem Coating Based on Lippia graveolens Essential Oil Loaded in Polymeric Nanocapsules.

Strawberries (Fragaria xannanasa) are susceptible to mechanical, physical, and physiological damage, which increases their incidence of rot during storage. Therefore, a method of protection is necessary in order to minimize quality losses. One way to achieve this is by applying polymer coatings. In this study, multisystem coatings were created based on polymer nanocapsules loaded with Lippia graveolens essential oil, and it was found to have excellent optical, mechanical, and water vapor barrier properties compared to the control (coating formed with alginate and with nanoparticles without the essential oil). As for the strawberries coated with the multisystem formed from the polymer nanocapsules loaded with the essential oil of Lippia graveolens, these did not present microbial growth and only had a loss of firmness of 17.02% after 10 days of storage compared to their initial value. This study demonstrated that the multisystem coating formed from the polymer nanocapsules loaded with the essential oil of Lippia graveolens could be a viable alternative to preserve horticultural products for longer storage periods.

Insect ear‐feeding impacts Gibberella ear rot and deoxynivalenol accumulation in corn grain

AbstractHigh deoxynivalenol (DON) levels in corn (Zea mays L.) is a grain quality issue for many growers in the US Great Lakes region. High DON levels can be attributed to the interaction of environment, pathogen (Fusarium spp. causing ear rot), and hybrid susceptibility. However, ear‐feeding insects can provide easy access for fungal infection and increase DON accumulation, hence the evaluation of insect protection strategies such as hybrid insecticidal proteins is crucial. Field trials were conducted at four locations in Michigan to study the impact of hybrids insect protection trait on ear injury, ear rot infection, and DON levels. Fungicide application (prothioconazole at silking stage) was conducted at two locations and included non‐treated control. Insect feeding incidence (IFI) and ear rot incidence (ERI) were correlated at three locations but were stronger at locations with environments not conducive to fungal growth soon after silking. Correlation between IFI and DON was observed only at locations with highest insect pressure. Hybrid with Vip3A protein reduced IFI by >70% at all locations but lowered DON concentration at one out of three tested locations compared to non‐Vip3A hybrids. Fungicide application reduced ear rot severity at one of two locations but did not impact ERI or DON levels. Overall, results showed that ear‐feeding by insects can lead to an increase in ear rot and DON levels, and use of hybrids with Vip3A protein can reduce DON levels especially under high insect pressure.

Use of Pseudomonas protegens to Control Root Rot Disease Caused by Boeremia exigua var. exigua in Industrial Chicory (Cichorium intybus var. sativum Bisch.).

Boeremia exigua var. exigua is a recurrent pathogen causing root rot in industrial chicory. Currently, there is no chemical or varietal control for this disease, and thus, management strategies need to be developed. This study determined the biocontrol effect of strains of Pseudomonas protegens bacteria with antimicrobial compounds on the fungus B. exigua var. exigua under in vitro, in vivo, and field conditions. In addition, root colonization by these bacteria was estimated by the phlD-specific PCR-based dilution end point assay. Eighteen isolates of Pseudomonas spp were evaluated, and the strains that showed the greatest in vitro inhibition of fungal mycelial growth (mm), Ca10A and ChB7, were selected. Inoculation with the strain ChB7 showed less severity (necrotic area) under in vivo conditions (root trials) compared with the control inoculated with the pathogen (p ≤ 0.05). The molecular analysis revealed that the root colonization of plants grown in pots was equal to or greater than 70%. Similar levels were observed in the field trials conducted at the Selva Negra and Canteras experimental stations (2015-2016 season), with values ranging from 85.7 to 70.5% and from 75.0 to 79.5%, respectively. Regarding yield (ton ha-1), values were higher in the treatments inoculated with strains Ca10A and ChB7 (p ≤ 0.05) at both experimental sites, while a lower incidence and severity of root rot were observed at Selva Negra. These results suggest that the Chilean strains of P. protegens are a promising tool for the control of root diseases in industrial chicory.

Fungal Microbiome Indicators Are Associated with Genotypic Variation in Pea Root Rot Susceptibility when Intercropped with Barley

Intercropping of legume and cereal crop species shows potential to reduce root disease pressures by changing root-associated microbiomes and improving nitrogen (N) use via soil N-dependent fixation of atmospheric N2 by symbiotic rhizobia. A two-year field study was conducted to evaluate the effect of pea–barley association on crop performance and on the root fungal community. Five pea cultivars (Alvesta, Karpate, Mytic, Respect, and Vitra) were grown either in pure stands or mixed with one variety of barley (Atrika). We measured crop grain yield and root rot incidence and analyzed root fungal communities. In mixed stands, total grain yield was more stable compared with that in each pure stand, but pea root disease incidence was higher except for cultivars Vitra and Karpate. The effect of cropping system on fungal alpha diversity depended on the cultivar, with Vitra showing higher Shannon diversity and Alvesta showing lower richness in mixed compared with pure stands. All four operational taxonomic units (OTUs) belonging to the Didymellaceae family were positively associated with pea root rot, and another disease-associated OTU in pea, Neoascochyta exitialis, was found to be also part of the barley core microbiome. Eleven of twelve OTUs belonging to the Glomeraceae family were associated with healthy roots and abundant in cultivar Vitra. This study shows how the phenotype and fungal microbiome of different pea cultivars respond distinctly to intercropping. Furthermore, the identification of disease- and health-associated taxa in the pea root fungal community refines the characterization of different cultivar candidates for intercropping. [Formula: see text] Copyright © 2024 The Author(s). This is an open access article distributed under the CC BY 4.0 International license .

Avocado (Persea americana cv. ‘Hass’) Fruit Mineral Composition at Canopy Level towards Sustainable Quality

Sustaining avocado fruit quality is crucial to maintain customer satisfaction and confidence. Among fruit qualities, mineral nutrient composition is an important contributor to postharvest robustness. Towards better understanding and addressing variability within the plant canopy, ‘Hass’ fruit from across seven orchard blocks were individually characterised. From five representative trees in each block, five fruit were harvested (one from each of five positions: top (sun-exposed), bottom (shaded), middle (shaded), East (sun-exposed), and West (sun-exposed)). Fruit dry matter was significantly higher (p ≤ 0.001) in fruit from the top, East, and West sun-exposed positions. No significant (p > 0.05) effect of position was discerned for fruit weight at harvest or for either stem end rot (SER) or body rot (BR) incidence at eating soft. Shaded fruit had significantly higher (p ≤ 0.05) [N], [K], [Mg], N:Ca, K:Ca, and K + Mg:Ca in their flesh. Significant negative linear correlations (p ≤ 0.001) were obtained between fruit DM and flesh [N] (r = −0.75), [K] (r = −0.67), and N:Ca (r = −0.57). SER and BR incidence were significantly positively correlated (p ≤ 0.01) with flesh and skin mineral ratios of N:Ca, K:Ca, Mg:Ca, and K + Mg:Ca. Skin and flesh [Ca] were significantly negatively correlated with SER (r = −0.51, p ≤ 0.01) and BR (r = −0.74, p ≤ 0.001) incidences. Soil cation (Ca, Mg, K) availability (%base saturation of cation exchange capacity (CEC)) was not (p > 0.05) correlated with skin or flesh mineral concentrations or ratios. Considered collectively, results suggest that selective harvest of sun-exposed fruit with inherently lower mineral nutrient ratios yields relatively robust fruit. Such fruit lots should better tolerate the rigours of harvest and postharvest treatment and handling. In this context, they should better maintain quality upon passage through long, in terms of accumulated time-temperature increments, export supply chains. In contrast, shaded fruit could be directed into shorter domestic supply chains. As a harvest strategy, segregating fruit lots from harvest could underpin the quality offered to consumers at the end of ‘short’ and ‘long’ supply chains.

Novel Multiresistant Osmotin-like Protein from Sweetpotato as a Promising Biofungicide to Control Ceratocystis fimbriata by Destroying Spores through Accumulation of Reactive Oxygen Species.

Osmotin-like proteins (OLPs) play an important role in host-plant defense. In this study, a novel multiresistant OLP (IbOLP1) was screened from sweetpotato (Ipomoea batatas) with a molecular weight of 26.3 kDa. The expression level of IbOLP1 was significantly higher in resistant cultivars than susceptible ones after inoculation with Ceratocystis fimbriata, which causes black rot disease in sweetpotato. The expression of IbOLP1 in Pichia pastoris led to the lysis of yeast cells themselves. The recombinant IbOLP1 displayed antifungal, antibacterial, and antinematode activity and stability. IbOLP1 could restrain the mycelial growth and lyse spores of C. fimbriata, distinctly reducing the incidence of black rot in sweetpotato. The IbOLP1 can trigger the apoptosis of black rot spores by elevating the intracellular levels of reactive oxygen species. Collectively, these findings suggest that IbOLP1 can be used to develop natural antimicrobial resources instead of chemical agents and generate new, disease-resistant germplasm.

Arbuscular mycorrhizal fungi association and root rot disease in mungbean (<i>Vigna radiata</i>) growing areas of Haryana

The study was carried out during rainy (kharif) seasons of 2021 and 2022 at Chaudhary Charan Singh Haryana Agricultural University, Hisar, Haryana to provide a comprehensive understanding of how the presence and activity of arbuscular mycorrhizal (AM) fungi may influence the development of root rot in mungbean (Vigna radiata L.) crops in different mungbean-growing areas of Haryana. Root colonization by AM fungi in Hisar, Bhiwani, Mahendragarh, and Charkhi Dadri districts of Haryana ranged from 16.45 to 24.07%, 7.71 to 16.73%, 7.02 to 21.43% and 7.59 to 22.28%, respectively. This variability underscores the diverse interactions between mungbean plants and AM fungi in these distinct geographical regions, reflecting the influence of environmental factors and local soil conditions on mycorrhizal associations. Sporocarp numbers in these districts ranged from 76.5 to 131.5, 37 to 102, 54 to 119.5 and 65.5 to 118 per 100 g of soil, respectively. These findings emphasize the dynamic nature of AM fungal populations and their potential impact on the overall soil health and plant-microbe interactions in mungbean cultivation. Similarly, root rot incidence in these districts varied from 6.59 to 11.62%, 7.48 to 11.29%, 7.05 to 11.43% and 5.88 to 11.76%, while root rot intensity ranged from 2.77 to 4.82%, 2.70 to 4.61%, 2.06 to 4.61% and 2.43 to 4.85% in Hisar, Bhiwani, Mahendragarh, and Charkhi Dadri districts, respectively. This variability in root rot incidence and intensity highlights the susceptibility of mungbean crops to this detrimental disease and emphasizes the importance of region-specific strategies for disease management.

Alternative management strategies reduced the incidence and severity of root rot of melon

Alternative management strategies reduced the incidence and severity of root rot of melon

First Report of Root Rot Caused by Fusarium oxysporum on Aralia elata in China.

Aralia elata (Miq.) Seem., is grown for its medicinal and nutritional properties in northeastern China. The tender shoots are used as wild vegetables. The plant saponin components have antioxidant and neuroprotective activities, and are used for the treatment of chronic disease (Xia et al. 2021). In July 2021, root rot disease was observed in five-year-old A. elata plants in Qingyuan County (41°91' N, 124°59' E), Liaoning Province, China. The incidence of roots rot was approximately 50% in old fields, with the leaves of the infected plants appearing chlorotic and wilting. The lesions on the taproots were dark brown and soft, with degraded internal organization. Leading edge of necrotic tissue from symptomatic roots was cut 5×5×3 mm, placed in 75% ethanol for 30 s, and then in 3% sodium hypochlorite for 2 min. After three rinses in sterile distilled water, the samples were dried on sterile filter paper before plating on potato dextrose agar (PDA) and incubation at 25℃. Monosporic cultures were obtained by the collection of single spores from individual isolates. After 7 days on PDA, mycelia in the colonies appeared cottony and pink, white, or purple in color, while their undersides were pink and white. Spore characteristics were evaluated after transfer to carnation leaf agar (CLA) and incubation for 20 days (Zhang et al. 2021). The macroconidia were falciform, slightly curved or straight, two to five septate, and 20.57 to 33.75 × 3.62 to 6.11 μm (n=40). The microconidia were ovoid or oval, zero to one septate, and 5.12 to 13.53 × 3.04 to 4.79 μm (n=40). Chlamydospores were globose to subglobose, intercalary or terminal, with an average diameter of 13.76 μm (n=40).To identify the pathogen, the internal transcribed spacer (ITS) region, large subunit (LSU) ribosomal DNA, and translation elongation factor 1 alpha (TEF-1α) gene were amplified using the respective primer pairs ITS1/ITS4, LR0R/LR7, and EF1-728F/EF1-986R (Cheng et al. 2020; Fu et al. 2019). Comparisons with GenBank, the sequences of ITS, LSU, and TEF-1 had 99 to 100% homology with Fusarium oxysporum (accessions numbers- MH707084, OQ380519, and GU250609, respectively). The sequences were deposited in GenBank: OP482273 (ITS), OP491955 (LSU), and OP503498 (TEF-1α). Maximum likelihood phylogeny of the identified sequences using MEGA-X software indicated that the isolate represented F. oxysporum. The taproots of 30 one-year-old A. elata were washed and inoculated with 1×106/ml of the conidial suspension for two hours, and another 30 used as controls with sterile water. After planting in sterilized forest soil in flowerpots (36×30 cm), the plants were grown in a greenhouse for two weeks at 25℃ with 14 h of light. It was found that 50% of the roots showed typical root rot symptoms, while the controls were asymptomatic. The pathogenicity test was repeated three times, and reisolation of F. oxysporum from the roots fulfilled Koch's postulates. This is the first report of root rot in A. elata caused by F. oxysporum in China and indicates the necessity for suitable management strategies to protect A. elata production.

The Influence of Sprinkler and Drip Irrigation on the Incidence and Severity of Bacterial Disease in Onions Grown in Northeast California

Overhead sprinkler irrigation and its effect on leaf wetness are often referenced as a key factor for bacterial disease spread in onions grown in arid climates. A study was conducted in 2021 and 2022 in Tulelake, California, to compare the incidence and severity of bacterial disease under sprinkler and drip irrigation. The experiment was a split-plot randomized complete block design with drip and solid-set sprinkler irrigation as the main plot treatments and inoculation and no inoculation with plant-pathogenic bacteria as the split-plot treatments. The entire study area was irrigated with solid-set sprinklers from planting until the five-leaf stage to facilitate uniform onion germination and chemigation of herbicides for weed control. After the five-leaf stage, irrigation treatments were imposed for the remainder of the growing season. Total irrigation water amount and irrigation frequency were similar for both irrigation methods. Foliar disease severity and the incidence of bulb rot were 170 and 186% higher, respectively, for solid-set sprinkler compared with drip irrigation. Leaf wetness within the plant canopy during onion bulbing was on average 36% higher in the solid-set sprinkler compared with drip irrigation treatment, whereas relative humidity and temperature were similar between irrigation methods. Drip irrigation maintained lower soil water tension and increased average bulb size by 9% compared with solid-set sprinkler irrigation. This study confirms historical observations that overhead irrigation can extend leaf wetness and increase bacterial disease in onions in arid production areas and highlights the potential benefits of the adoption of drip irrigation.

Effect of cinnamaldehyde on Rhizopus stolonifer and on the conservation of sweet potato

Abstract Objectives Rhizopus stolonifer, which causes soft rot of sweet potato, has resulted in substantial loss of economy postharvest. Materials and Methods The antifungal effect and mechanism of cinnamaldehyde (CIN) against R. stolonifer were explored by biochemical analysis and RNA-sequencing in this research, and the edible quality of CIN-treated sweet potato was evaluated. Results The results showed that CIN inhibited the growth of R. stolonifer and reduced the incidence of soft rot in sweet potato at a minimum inhibitory concentration of 1000 μL/L. The damage of the cell walls of R. stolonifer by CIN was associated with the upregulation of CHT1. Cytosolic leakage and malondialdehyde content increased following CIN-treatment, which was correlated with increased reactive oxygen species levels and decreased catalase activity. Upregulation of ERG genes and oxidative stress-related genes following CIN treatment was associated with impaired cell membrane integrity. The mitochondrial structure of R. stolonifer was damaged by CIN treatment resulting in decreased respiration rates and ATP production, which was correlated with lower expression levels of IDH1, COX4, and QCR7. Conclusions Our research suggests that the inhibition of CIN on R. stolonifer was related to the disruption of its normal gene expression network, and CIN maintained the nutritional and edible quality of sweet potato. This study provides valuable insights into the antifungal mechanism of CIN on R. stolonifer and the maintenance of root quality, which contributes to the efficient use of CIN for controlling soft rot in sweet potato.

Effect of essential oils, medicinal plants, bio agents and chemicals on sheath rot incidence and yield of rice

Effect of essential oils, medicinal plants, bio agents and chemicals on sheath rot incidence and yield of rice

Ascorbic acid combined with chitosan edible coating as alternative treatment for sulfur dioxide to control rot incidence and keeping the quality of ‘Flame Seedless’ grapes during short supply chains

Ascorbic acid combined with chitosan edible coating as alternative treatment for sulfur dioxide to control rot incidence and keeping the quality of ‘Flame Seedless’ grapes during short supply chains

Identification and Pathogenicity of Rhizoctonia solani Isolates Causing Leaf and Stem Rot in Three-Leaf Ladybell

In 2020 and 2021, we surveyed diseases of three-leaf ladybell (<i>Adenophora triphylla</i>) plants grown in fields at two locations in Korea. During the disease surveys, severe leaf rot symptoms were observed on the young plants in Hongseong, and stem rot symptoms on the adult plants in Cheolwon. The incidence of leaf rot was 5‒60%, and that of stem rot 1‒10%. We obtained 6 fungal isolates each from the leaf rot lesions and the stem rot lesions. All the isolates were morphologically identified as <i>Rhizoctonia solani</i>. Anastomosis test and investigation of cultural features of the fungal isolates revealed that the isolates from the leaf rot lesions corresponded to <i>R. solani</i> AG-1(IB), and those from the stem rot lesions to <i>R. solani</i> AG-2-2(IIIB). Two isolates each of <i>R. solani</i> AG-1(IB) and AG-2-2(IIIB) were used for DNA sequence analysis and pathogenicity test to three-leaf ladybell plants through artificial inoculation. The anastomosis groups and cultural types of the <i>R. solani</i> isolates were confirmed by the sequence analysis. The pathogenicity tests revealed that the isolates of <i>R. solani</i> AG- 1(IB) caused only leaf rot symptoms on the inoculated plants, and those of <i>R. solani</i> AG-2-2(IIIB) leaf rot and stem rot symptoms. The induced symptoms were similar to those observed in the fields investigated. Leaf and stem rot of three-leaf ladybell caused by the two anastomosis groups and cultural types of <i>R. solani</i> is first reported in this study.

Developing Biocontrol Strategies for the Management of Set rot Disease of Sugarcane under Wider Row Planting

Sugarcane (Saccharum officinarum L.) is one of the economically valuable agricultural crops grown worldwide in tropical and subtropical areas mainly for their sugar source. Among the 110 sugarcane cultivated countries, India and Brazil contribute half of global production [1]. During the period of last five decades the production, productivity and sugarcane recovery has also shown remarkable resilience in productivity growth rate. One of the reasons for the low productivity and recovery is increasing incidence of insect pests and diseases. An ecologically friendly alternative to these problems is biological control using rhizobacteria and their metabolic products [2]. In order to develop a technology capsule Surveys and surveillance were conducted in sugarcane fields both in plant and ratoon crops distributed in Orthanadu and Vallam block in New Cauvery delta area of Thanjavur dictrict during three consecutive years 2019- 22 with an objective to assess the incidence of sett rot, red rot and smut in New Cauvery delta area of Thanjavur dictrict. Fortnightly fixed plot observation was taken. The Maximium and minimum temperature, RH and quantity of rain and rainy days also recorded daily and consolidated on monthly basis and weather parameters were correlated with the incidence of disease. The results revealed that the maximum incidence sett rot negatively correlated with temperature. Similarly the number of rainy days and rainfall is positively correlated (r = 0.7523) with incidence of sett rot. For managing sett rot disease of sugarcane through biological approach, studies were conducted on biocontrol agents viz., Trichoderma asperellum (TNAU), Bacillus subtilis (TNAU), Chaetomium globosum (Cg6) with fungicide check propiconozole 0.1%. Among the treatment, highest millable cane (91,080/ha) and yield (91.39 tonnes / ha) and sugar yield ( 10.63 tonnes/ha) were recorded in M1- (Single bud treated with Trichoderma asperellum (TNAU)@ 4 g l-1 + soil application @ 2.5 kg ha-1) followed by M2- (Single bud treated with Bacillus subtilis (TNAU)@ 10 g l-1 + soil application @ 2.5 kg ha-1) in which, the cane yield of 88.54 tonnes/ha and sugar yield of 10.68 tonnes/ha were recorded. This study provides a technology capsule for the management of important disease of sugarcane.

Efficacy of Fungicides and Optimization of Application Timing for the Management of Sclerotinia Rot of Mustard Caused by Sclerotinia sclerotiorum

Sclerotinia rot of rapeseed-mustard caused by Sclerotinia sclerotiorum (Lib.) de Bary has gained significant importance in Rajasthan, Punjab, Haryana and other major rapeseed-mustard growing areas of India in recent years. Fungicides are extensively used for the control of this disease since no commercial cultivars have been found resistant to S. sclerotiorum and other management practices have not been found very effective. Therefore, the present investigations were carried out to evaluate the efficacy of fungicides and to optimize application timing for the effective management of Sclerotinia rot of mustard. Among 08 fungicides tested in vitro, Carbendazim 50% WP and Propiconazole 25% EC were found most efficient at completely inhibiting the mycelial growth of S. sclerotiorum at all the concentrations tested. In an in vitro sclerotial (carpogenic) germination test, Carbendazim 50% WP completely inhibited sclerotial germination at 100, 250 and 500 ppm. Under artificial inoculation conditions in the field, Propiconazole 25% EC @0.1% was found most effective in reducing Sclerotinia rot incidence (87.04%) followed by Carbendazim 50% WP (83.33%) and Tebuconazole 25.9% EC (75.93%). Similarly, under natural epiphytotic conditions in field, Propiconazole 25% EC @0.1% was found best in disease incidence reduction (85.34%) with increased yield (40.00%) followed by Carbendazim 50% WP (81.25% & 38.09%) and Tebuconazole 25.9% EC (79.72% & 35.24%) in disease reduction and increased yield respectively. Differential time and frequency of application of Propiconazole @0.1% were tested and found that 02 applications i.e. 1st spray at 4.2 flowering stage i.e. 30% bloom stage (70 DAS) and 2nd spray at 4.3 flowering stage i.e. 50% bloom stage (85 DAS) was equally effective in disease incidence reduction (82.70%) with 03 applications i.e. 1st spray at 4.1 flowering stage i.e. 10-20% bloom stage (55 DAS), 2nd at 4.2 flowering stage (70 DAS) and 3rd at 4.3 flowering stage (85 DAS). Therefore, the optimization of fungicide application timing and the number of sprays with adequate concentrations is advisable to increase the cost-effectiveness of crop production while controlling the disease.

Integrated Management of Charcoal Rot and Its Influence on Seed Yield and Quality in Soybean

Charcoal rot (Macrophomina phaseolina) causes high grain yield loss in major soybean-growing nations worldwide. The present study aimed to reveal the effect of stage-wise charcoal rot incidence and their root and stem severity index on the yield criteria of soybean varieties. Additionally, an attempt was made to derive integrated management practices and their influence on seed quality. All the twelve varieties were affected by charcoal rot, and its incidence and root and stem severity index were high in Shivalik (48.5% and 3.7, respectively). In yield estimation, the percentage of yield loss ranged from 8.7% (JS 20-98) to 53.9% (Shivalik). Percent yield loss had a strong significant positive relation with percent incidence (0.912**) and Root and Stem severity index (0.813**) of charcoal rot. Seed treatment of Penflufen 13.28% + Trifloxystrobin 13.28 % FS @ 1ml/kg followed by spraying of tebuconazole 25% EC @ 0.1 % spraying at 45,60,75 days was significantly superior in reducing charcoal rot (19.7%) in comparison to untreated (37.3%). The highest seed germination (80.0 %) and least association with M. phaseolina (13.33%), Aspergillus flavus (3.33%), Fusarium sp., (13.34%) in comparison to control (50, 36.67, 23.33 and 36.67 %, respectively) were also resulted from the seed obtained from above treatment. In bioagent, seed treatment of T. harzianum @ 10 gm/kg followed by foliar spray of tebuconazole 25% EC @ 0.1% at 45,60,75 days was the second-best combination among all treatments in all respects. The effect of these fungicides and bio agents as seed treatment also improved the number of branches, pods, 100 seed weight and yield. Hence, these combinations could be applied to minimize charcoal rot and yield losses in soybean.

Enhancing sugar beet yield and quality in Northeast China: Investigating the synergistic impact of sugar mill filter mud and biochar on black soil

In this study, different ratios of sugar beet sugar factory filter mud and biochar in different proportions were used to assess the effect of incorporating filter mud into the field on soil quality and its influence on two distinct sugar beet (Beta vulgaris) varieties: high-yielding KWS9147 and high-sugar KWS1176. The experiment was conducted in 2022 at the Hulan Campus of Heilongjiang University in Harbin City, Heilongjiang Province, China. Eight treatments were employed, including CK1 (no filter mud or biochar application), CK2 (20 t/ha biochar), T1 (16.67kg/ha filter mud), T2 (16.67 t/ha filter mud + 20 t/ha biochar), T3 (33.33 t/ha filter mud), T4 (33.33 t/ha filter mud + 20 t/ha biochar), and T5 (50kg/ha filter mud). For sugar beets, parameters such as the SPAD value(Soil and Plant Analyzer Development, represents the relative content of chlorophyll), disease resistance (root rot and brown spot), tuber yield, and sugar content were evaluated. Soil analysis included nitrogen content, available potassium content, available phosphorus content, and soil sucrase, urease, and phosphatase activities. Results indicated that the combined application of 16.67 t/ha filter mud and 20 t/ha biochar had a positive impact on the growth indices, tuber yield, sugar content, effective phosphorus, and quick-acting potassium content in KWS1176. In contrast, for KWS9147, the most favorable effect was achieved with the application of 16.67 t/ha filter mud. This treatment improved plant leaf growth, increased tuber yield and sugar content, reduced root rot incidence, elevated alkaline dissolved nitrogen content in the inter-root soil, and enhanced soil phosphatase activity. Biochar application did not contribute significantly to these improvements. Therefore, returning sugar mill sludge to the field demonstrated its potential to enhance soil quality, promote the yield of sugar beet varieties, and improve resistance to root rot while also providing an eco-friendly solution for managing environmental issues related to sludge accumulation.