Objective: The purpose of this study was to investigate the association between premenstrual syndrome (PMS) and daily physical activity. Methods: Three hundred forty-nine women (18-50 years) were analyzed. We investigated body mass index, PMS symptoms, physical activity level, and some factors related to PMS (age, sleep time, caffeine intake, alcohol intake, smoking status). Participants were grouped according to physical activity level into low, normal, and high physical activity groups. Binominal logistic regression analysis was used to examine the association between PMS and daily physical activity level. Results: The average physical activity levels of the low, normal and high physical activity groups were 301.4 ± 233.8 kcal, 975.0 ± 187.3 kcal, and 4558.7 ± 3798.5 kcal, respectively. The incidence of PMS was higher in both the low physical activity group (OR=2.45, 95% CI=1.18-5.11) and high physical activity group (OR=2.13, 95% CI=1.01-4.50) than in the normal physical activity group. Conclusion: PMS rates were higher in women who have either low or high daily physical activity levels than in those with normal physical activity levels. Therefore, women should be advised to avoid inactivity or excessive daily physical activity.