Inactivation Of Fungal Spores Research Articles

Impact of metal ions on PMS/Cl− disinfection efficacy: Enhancing or impeding microbial inactivation?

Peroxymonosulfate (PMS) is an eco-friendly disinfectant gaining attention. This study examined the influence of metal ions (Co(II), Cu(II), Fe(II)) on PMS disinfection with chloride ions (Cl−) against waterborne microorganisms, encompassing both bacteria and fungal spores. The findings elucidated that metal ions augment the inactivation of bacteria in the PMS/Cl− system while concurrently impeding the inactivation of fungal spores. Specifically, the PMS/Co(II)/Cl− process increased E. coli inactivation rates by 2.25 and 2.75 times compared to PMS/Co(II) and PMS/Cl−, respectively. Conversely, PMS/Me(II)/Cl− generally exhibited a diminished inactivation capacity against the three fungal spores compared to PMS/Cl−, albeit surpassing the efficacy of PMS/Me(II). For instance, the inactivation levels of A. niger by PMS/Cl−, PMS/Cu(II)/Cl−, and PMS/Cu(II) are 4.47-log, 1.92-log, and 0.11-log, respectively. Notably, fungal spores demonstrated a substantially higher resistance to disinfectants compared to bacteria. Differences in microbial susceptibility were linked to cell wall structure, composition, antioxidant defenses, and reactive species generation, such as hydroxyl radicals (•OH), sulfate radicals (SO4•−), and reactive chlorine species (RCS). This study demonstrated the novel and unique phenomenon of metal ions' dual role in modulating the PMS/Cl− disinfection process, which has not been reported before and has important implications for the field of water treatment.

Inactivation of fungal spores using household BDD device as a POU purification device: Application evaluation, influencing factors, and mechanisms

Fungal contamination is commonly found in water supply systems, with filamentous fungi posing a particular threat through secondary pollution and potential risks to drinking water safety. In this study, an electrolytic device, with boron-doped diamond (BDD) as the anode material, was proposed for use as a point-of-use (POU) water treatment device to address the fungal contamination in tap water. The BDD device was found to effectively inactivate Aspergillus terrestris spores under different processing current intensities, water hardness, and alkalinity conditions. Moreover, in practical tap water applications, it also exhibited effective inactivation of Aspergillus terrestris spores and good efficiency in removing antibiotic-resistant genes (ARGs). During the electrolysis, various active species are produced, including ozone, reactive chlorine, and extracellular reactive oxygen species (eROS) such as •OH, •O2− and •1O2. Addtionally, spores produce excessive intracellular reactive oxygen species (iROS) under stimulus. These species could damage the cell membrane, resulting in DNA and TN leakage, a decrease in cellular ATP content, and destruction of surface morphology. This study provides a novel solution for eliminating fungal contamination in water endpoints.

Rapid Inactivation of Fungal Spores in Drinking Water by Far-UVC Photolysis of Free Chlorine.

Effective and affordable disinfection technology is one key to achieving Sustainable Development Goal 6. In this work, we develop a process by integrating Far-UVC irradiation at 222 nm with free chlorine (UV222/chlorine) for rapid inactivation of the chlorine-resistant and opportunistic Aspergillus niger spores in drinking water. The UV222/chlorine process achieves a 5.0-log inactivation of the A. niger spores at a chlorine dosage of 3.0 mg L-1 and a UV fluence of 30 mJ cm-2 in deionized water, tap water, and surface water. The inactivation rate constant of the spores by the UV222/chlorine process is 0.55 min-1, which is 4.6-fold, 5.5-fold, and 1.8-fold, respectively, higher than those of the UV222 alone, chlorination alone, and the conventional UV254/chlorine process under comparable conditions. The more efficient inactivation by the UV222/chlorine process is mainly attributed to the enhanced generation of reactive chlorine species (e.g., 6.7 × 10-15 M of Cl•) instead of hydroxyl radicals from UV222 photolysis of chlorine, which is verified through both experiments and a kinetic model. We further demonstrate that UV222 photolysis damages the membrane integrity and benefits the penetration of chlorine and radicals into cells for inactivation. The merits of the UV222/chlorine process over the UV254/chlorine process also include the more effective inhibition of the photoreactivation of the spores after disinfection and the lower formation of chlorinated disinfection byproducts and toxicity.

Inactivation of fungal spores by performic acid in water: Comparisons with peracetic acid

Performic acid (PFA) has received increasing attention in water disinfection due to its high disinfection efficiency and fewer formation of disinfection by-products. However, the inactivation of fungal spores by PFA has not been investigated. In this study, the results showed that the log-linear regression plus tail model adequately described the inactivation kinetic of fungal spores with PFA. The k values of A. niger and A. flavus with PFA were 0.36 min−1 and 0.07 min−1, respectively. Compared to peracetic acid, PFA was more efficient in inactivating fungal spores and caused more serious damage on cell membrane. Compared to neutral and alkaline conditions, acidic environments demonstrated a greater inactivation efficiency for PFA. The increase of PFA dosage and temperature had a promoting effect on the inactivation efficiency of fungal spores. PFA could kill the fungal spores by damaging cell membrane and penetration of cell membranes. In real water, the inactivation efficiency declined as a result of the existence of background substances such as dissolved organic matter. Moreover, the regrowth potential of fungal spores in R2A medium were severely inhibited after inactivation. This study provides some information for PFA to control fungi pollution and explores the mechanism of PFA inactivation.

Pre-exposure of peracetic acid enhances its subsequent combination with ultraviolet for the inactivation of fungal spores: Efficiency, mechanisms, and implications

Waterborne fungi pose a potential threat to water supply safety due to their high resistance to disinfectants. Peracetic acid, as a promising alternative disinfectant to chlorine, has attracted increasing attention in water treatment. In this study, the inactivation of two dominant fungal species (Aspergillus niger and Aspergillus flavus) by sequential application of peracetic acid and ultraviolet (PAA-UV/PAA) was reported for the first time. Results revealed that the pre-exposure of PAA could facilitate the subsequent process of UV/PAA combination and shorten the lag phase in fungi inactivation. After 10 min of PAA pre-exposure, PAA-UV/PAA achieved 3.03 and 2.40 log inactivation of Aspergillus niger and Aspergillus flavus, which were 2- and 4.3-fold higher than that of direct UV/PAA under the same UV and PAA doses. PAA-UV/PAA disinfection also exhibited a stronger regrowth inhibition for incompletely inactivated fungal spores than direct UV/PAA. The increase of pH (5.0–9.0) and humic acid concentration (1.0–5.0 mg L − 1) showed an inhibitory effect on PAA-UV/PAA inactivation, but PAA-UV/PAA was more adaptable in a wide pH range and the presence of humic acid compared to direct UV/PAA. The more severe cell membrane damage and higher reactive oxygen species level in PAA-UV/PAA were evidenced for the first time by flow cytometry. The increased hydroxyl radical generation and higher synergism were primarily responsible for inactivation improvement. This study enhances the further understanding of the PAA-UV/PAA process, and the findings are expected to promote the development of PAA as a promising disinfectant for effective fungi control.

Enhanced solar inactivation of fungal spores by addition of low-dose chlorine: Efficiency and mechanism

This work demonstrated that the solar inactivation of fungal spores was enhanced by addition of low-dose chlorine. Although the effect of low-dose chlorine alone (2.0 mg/L) on culturability of fungal spores was negligible, the solar/chlorine inactivation on fungal spores performed better than solar alone inactivation, with a lower shoulder length and a higher maximum inactivation rate constant. The enhanced inactivation of Aspergillus niger can be ascribed to the membrane oxidation by chlorine, and the enhanced inactivation of Penicillium polonicum can be ascribed to the membrane oxidation by chlorine and ·OH (·OH plays a major role). The oxidization by chlorine and ·OH led to an increase in membrane permeability of fungal spores, which enhanced the solar inactivation, resulting in an increase in intracellular ROS and more serious morphological damage. Due to the presence of background substances such as dissolved organic matter and metal ions (Fe2+, Mn2+, etc.), the inactivation efficiency in real water matrices was decreased. The main disinfection by-products (DBPs) produced in the inactivation of fungal spores in chlorine alone and solar/chlorine treatments were dichloroacetic acid, trichloroacetic acid, trichloroacetone and trichloromethane. Generally, DBPs formation in solar/chlorine treatment was lower than those in chlorine alone treatment. Moreover, the regrowth potential of the two genera of fungal spores in R2A medium could be inhibited by adding low-dose chlorine.

Inactivation of fungal spores in water by CuO-activated peracetic acid: Kinetics, mechanism and regrowth

The disinfection of pathogenic microorganisms in water treatment by peracetic acid (PAA)-based advanced oxidation processes (AOPs) has been gaining increasing concern. In this work, the inactivation mechanism, influencing factors and regrowth of two pathogenic Aspergillus species in the system of CuO-activated PAA were studied for the first time. The k values of A. niger and A. flavus inactivated by PAA/CuO system were 3.9 and 2.1-fold higher than those inactivated by PAA alone. PAA concentration and CuO dose were positively correlated with the inactivation efficiency, while humic acid and pH were negatively correlated. The main active species that contributed to the inactivation of fungal spores in PAA/CuO system were •OH, CH3C(O)OO• and 1O2. PAA/CuO system had more intense oxidative stimulation and more serious damage to fungal spores according to the analysis of cell membrane integrity and intracellular ROS levels. In addition, the PAA/CuO system was less impacted by the water matrix and kept a good inactivation efficiency in real water samples. The regrowth potential of fungal spores after disinfection was also reduced in PAA/CuO system so as to avoid the risk of biological regrowth. This study provides a feasible PAA-based advanced oxidation method for activating PAA and inactivating fungal spores.

Effective inactivation of fungal spores by the combined UV/PAA: Synergistic effect and mechanisms

Peracetic acid (PAA) can effectively inactivate fungi in water, while may pose a potential risk of regrowth after disinfection. The inactivation kinetic and mechanism of fungal spores by combined UV and PAA (UV/PAA) was investigated in this study. The results showed that synergistic factor of the inactivation of A. niger and A. flavus was 1.44 and 1.37, which indicated significant synergistic effect of UV/PAA. The k of A. niger and A. flavus was similar at pH 5.0 and 7.0, while decreased 60.00% and 39.13% at pH 9.0 compared with that at pH 7.0. The effect of HA concentration on the inactivation efficiency of fungal spores by UV/PAA was negative, while the effect of PAA concentration was positive. The membrane permeabilized cell of A. niger and A. flavus caused by UV/PAA was 17.0% and 31.7%, which was higher than that caused by PAA and UV alone. The changes of morphology of fungal spores and the leakage of intracellular material indicated that the damage of cell structure caused by UV/PAA system was more serious than that of UV or PAA alone. In addition, the four parts that contributed in UV/PAA system was in the following order: UV > radical > PAA > synergistic effect. The inactivation efficiency of combined UV and chlorine (UV/Cl2) was higher than that of UV/PAA. Furthermore, the typical order of the inactivation efficiency in different matrix was: phosphate buffer solution > surface water > secondary effluent. The regrowth potential of fungal spores after UV/PAA treatment was significantly lower than that by PAA alone, indicating that UV/PAA could decrease the microbial regrowth potential after PAA disinfection alone.

Sequential use of UV-LEDs irradiation and chlorine to disinfect waterborne fungal spores: Efficiency, mechanism and photoreactivation

In this work, sequential applications of light-emitting diodes (UV-LEDs) with two wavelengths and chlorine (Cl2) were performed for fungal spores disinfection: UV-Cl2, Cl2-UV, UV/Cl2-UV, UV-UV/Cl2, Cl2-UV/Cl2-Cl2. Overall comparisons of the sequential processes with respect to the inhibitory effect on photoreactivation were also evaluated. According to the evaluation of culturability and membrane permeability, inactivation of fungal spores by UV was not enhanced by prior or post exposure to Cl2, but in the UV/Cl2 process with pre or post UV treatment, the inactivation efficiency was greatly enhanced. Take P. polonicum for example, pre-treatments by UV265 and UV280 (40 mJ/cm2) caused the log count reduction (LCR) of 1.05 log and 0.95 log, then the followed UV265/Cl2 and UV280/Cl2 at the same UV fluence caused additional LCR of 1.80 log and 2.00 log. The permeabilization of P. polonicum was also accelerated in the processes of UV/Cl2-UV and UV-UV/Cl2, especially at the wavelength of 280 nm. In the sequential processes, especially those containing UV/Cl2 or at the wavelength of 280 nm, could promote the formation of intracellular reactive oxygen species (ROS), thus leading to more severe damage to the spores as reflected in the culturability reduction, membrane permeability and inhibition of photoreactivation.

Inactivation of fungal spores from clinical environment by silver bio-nanoparticles; optimization, artificial neural network model and mechanism

The present study aimed to assess the efficiency of silver bio-nanoparticles (Ag-NPs) in inactivating of the Aspergillus fumigatus, A. parasiticus and A. flavus var. columnaris and A. aculeatus spores. The AgNPs were synthesized in secondary metabolic products of Penicillium pedernalens 604 EAN. The inactivation process was optimized by response surface methodology (RSM) as a function of Ag NPs volume (1–10 μL/mL); time (10–120 min); pH (5–8); initial fungal concentrations (log10) (3–6). The artificial neural network (ANN) model was used to understand the behavior of spores for the factors affecting inactivation process. The best conditions to achieved SAL 10−6 of the fungal spores were recorded with 3.46 μl/mL of AgNPs, after 120 min at pH 5 and with 6 log of initial fungal spore concentrations, at which 5.99 vs. 6.09 (SAL 10−6) log reduction was recorded in actual and predicted results respectively with coefficient of 87.00%. The ANN revealed that the timehas major contribution in the inactivation process compare to Ag NPs volume. The fungal spores were totally inactivated (SAL 10−6, 6 log reduction with 99.9999%) after 110 min of the inactivation process, 10 min more was required to insure the irreversible inactivation of the fungal spores. The absence of protease and cellulase enzymes production confirm the total inactivation of the fungal spores. FESEM analysis revealed that the AgNPs which penetrated the fungal spores leading to damage and deform the fungal spore morphology. The AFM analysis confirmed the total spore surface damage. The bands in the range of the Raman spectroscopy from 1300 to 1600 cm−1 in the inactivated spores indicate the presence of CH3, CH2 and the deformation of lipids released outside the spore cytoplasm. These finding indicate that the AgNPs has high potential as a green alternative inactivation process for the airborne fungal spores.

Solar disinfection of fungal spores in water: Kinetics, influencing factors, mechanisms and regrowth

Water contamination with fungi has gained attention increasingly, while solar disinfection (SODIS) is a potential method, especially for developing countries. In this work, inactivation kinetics, influencing factors, mechanisms and regrowth of fungal spores in the SODIS process were firstly investigated. The inactivation fitted well with the Geeraerd and Van Impe inactivation model-fitting tool and P. polonicum was more sensitive to simulated sunlight than A. niger. The pH value within the range of 5–9 and low concentration humic acid did not affect the solar inactivation of fungal spores, while HA at the concentration above 5.0 mg/L inhibited the solar inactivation efficiency. Over the tested range, the inactivation efficiency was obtained at the temperature near the optimal growth temperature (30-40 °C for A. niger, 20–30 °C for P. polonicum). According to the results of flow cytometry, ATP concentration and scanning electron microscopy, the solar inactivation of fungal spores was ascribed to endogenous actions. During the SODIS process, the DNA and respiratory chain of fungal spores were attacked. Subsequently, esterase activity was induced. As a result, the spores lost the culturability firstly, accompanied by the exhaustion of ATP, finally the membrane became permeable. Although no fungal spore regrowth was observed in ground water and surface water after SODIS, they remained a certain regrowth potential in nutrient-rich water like R2A. This work uncovered the control of waterborne fungi by SODIS.

Inactivation of fungal spores in water with peracetic acid: Efficiency and mechanism

In recent years, peracetic acid (PAA) has been widely used in sewage disinfection due to its strongly sterilizing capacity and less toxic by-products production. However, there was no studies on the inactivation kinetics and mechanism of fungal spores in water by PAA as well as their regrowth. The inactivation efficiency and mechanism of Aspergillus niger and Aspergillus flavus by PAA was illustrated in this study. The results showed that the inactivation of fungal spores fitted to the delayed Chick–Watson model well. The inactivation rate constants were 0.038 and 0.033 L mg−1 min−1, respectively. The effects of pH value and humic acid concentration on the inactivation efficiency were negative and the effect of temperature was positive, while the effect of HCO3− and Cl− on the disinfection of fungal spores could be negligible. The results of flow cytometric measurements, morphological observation and the leakage of intracellular substances indicated that the inactivation of fungi by PAA was attributed to the penetration of cell membranes rather than the damage of cell membranes. PAA was found to be effective for the inactivation of fungal spores, but was less effective than several traditional disinfectants (Cl2, ClO2) under the same condition. Furthermore, the regrowth of fungal spores after disinfection with PAA was also evaluated. The regrowth of fungal spores that after 1–3 log inactivated in the R2A medium within 5 days was observed, but the regrowth of completely inactivated fungal spores in R2A and in trace element solution was negligible. This study provides the valuable information for the application of PAA in the control of fungal pollution.

Synergistic effect of ozone and chlorine on inactivating fungal spores: Influencing factors and mechanisms

Effective control of fungal contamination in water is vital to provide healthy and safe drinking water for human beings. Although ozone was highly effective in inactivating fungi in water, it was limited by a lack of continuous disinfection ability in water supply system. In present study, the inactivation of fungal spores by combining ozone and chlorine was investigated. The synergistic effects of Aspergillus niger and Trichoderma harzianum spores reached 0.47- and 0.55-log within 10 min, respectively. The inactivation efficiency and the synergistic effect would be affected by disinfectant concentration, pH, and temperature. The combined inactivation caused more violent oxidative stimulation and more severe damage to the fungal spores than the individual inactivation based on the flow cytometry analysis and the scanning electron microscopy observation. The synergistic effect during the combined inactivation process was attributed to the generation of hydroxyl radicals by the reaction between ozone and chlorine and the promotion of chlorine penetration by the destruction of cell wall by ozone. The combined inactivation efficiency in natural water samples was reduced by 26.4–43.8% compared with that in PBS. The results of this study provided an efficient and feasible disinfection method for the control of fungi in drinking water.

Antifungal activity of TiO2/AgBr nanocomposites on some phytopathogenic fungi.

TiO2/AgBr composites were synthesized by a simple ultrasonic strategy. Various instruments such as SEM, EDX, XRD, and FT‐IR were exploited to investigate their characteristics. Antifungal activities of the as‐obtained samples were assessed through the inactivation of Fusarium graminearum in the spore suspension method and mycelial growth inhibition of F. graminearum, Botrytis cinerea, and Sclerotinia sclerotiorum in the microdilution method. The results represented that the TiO2/AgBr samples possess higher antifungal activities on F. graminearum spores than the pure TiO2. The sample with 20 wt% silver bromide represented the highest inhibitory effect on the growth of F. graminearum so that all fungal spores were degraded in the initial times of the treatment process. The inactivation of fungal spores after 60 min was 35.2%, 97.8%, 98.9%, and 98.7%, in respect, for 5, 10, 20, and 30 weight percent of AgBr in the binary nanocomposites, while the inhibition rate was 13.4% for the pure TiO2. With increasing ultrasound irradiation time for more than 30 min, the inactivation rate constant decreased. It was also found that the antifungal activity of the nanocomposites without calcination was higher than those of the calcined materials. Considering the antifungal potential against phytopathogenic fungi and advantages such as simple synthesis and eco‐friendly nature, it seems that TiO2/AgBr nanocomposites can be used instead of synthetic chemicals after additional field investigations and mass production.

Inactivation of waterborne fungal spores by 1-bromo-3-chloro-5,5-dimethylhydantoin: Kinetics, influencing factors and mechanisms

Fungal contamination in drinking water source has become a problem worth studying, as waterborne fungi may cause deterioration of water quality and outbreak of diseases. Various disinfection methods have been explored to control fungal spores in drinking water, such as chlor(am)ination, ozonation, chlorine dioxide treatment, but these methods are not appropriate for remote areas, owing to the difficulties in preparation, carriage and storage. In this study, a powdery disinfectant of 1-bromo-3-chloro-5,5-dimethylhydantoin (BCDMH), which facilitated transportation and preservation, was firstly chosen to inactivate opportunistic pathogens of Aspergillus niger (A. niger) and Penicillium polonicum (P. polonicum). The results revealed that the inactivation kinetics of fungal spores by BCDMH fitted to Chick-Watson model well, with the inactivation rate constant of 0.011 and 0.034 L mg−1 min−1 for A. niger and P. polonicum, respectively. Acidic condition and high temperature promoted the inactivation by BCDMH. Compared with chlorine, BCDMH showed relative weaker ability on inactivation of fungal spores. However, it was demonstrated that the inactivation efficiency of BCDMH was obviously enhanced by adding halide ions, with 11 or 36 folds for A. niger and 4 or 15 folds for P. polonicum by adding 40 μM Br− or I−. The inactivation mechanisms were detected by flow cytometry and scanning electron microscope. Fungal spores lost their culturability firstly, then membrane integrity was damaged. Meanwhile, the esterase activity and intracellular reactive oxygen species level changed, and finally intracellular adenosine triphosphate released.

Evaluation of the vital viability and their application in fungal spores’ disinfection with flow cytometry

More attention was focused on fungi contamination in drinking water. Most researches about the inactivation of fungal spores has been conducted on disinfection efficiency and the leakage of intracellular substances. However, the specific structural damage of fungal spores treated by different disinfectants is poorly studied. In this study, the viability assessment methods of esterase activities and intracellular reactive oxygen species (ROS) were optimized, and the effects of chlorine-based disinfectants on fungal spores were evaluated by flow cytometry (FCM) and plating. The optimal staining conditions for esterase activity detection were as follows: fungal spores (106 cells/mL) were stained with 10 μM carboxyfluorescein diacetate and 50 mM ethylene diamine tetraacetic acid at 33 °C for 10 min (in dark). The optimal staining conditions for intracellular ROS detection were as follows: dihydroethidium (the final concentration of 2 μg/mL) was added into fungal suspensions (106 cells/mL), and then samples were incubated at 35 °C for 20 min (in dark). The cell culturability, membrane integrity, esterase activities, and intracellular ROS were examined to reveal the structural damage of fungal spores and underlying inactivation mechanisms. Disinfectants would cause the loss of the cell viability via five main steps: altered the morphology of fungal spores; increased the intracellular ROS levels; decreased the culturability, esterase activities and membrane integrity, thus leading to the irreversible death. It is appropriate to assess the effects of disinfectants on fungal spores and investigate their inactivation mechanisms using FCM.

Inactivation of fungal spores in water using ozone: Kinetics, influencing factors and mechanisms

Fungal contamination of drinking water sources is increasingly threatening the environment and human health. In this study, the inactivation of three genera of dominant fungi in drinking water sources using ozone was first reported. The inactivation of the fungal spores by ozone could be divided into two distinct stages: first a rapid reduction in survival, and then the inactivation at a slower rate. The secondary stage inactivation fitted the Chick–Watson model well, and there was no significant difference in the second-order inactivation rate constants of the three fungal spores (0.199–0.209 L mg−1 min−1). The inactivation rate constants of fungal spores by molecular ozone were much lower than those of viruses, which were equivalent to that of Cryptosporidium. The increase in pH and temperature showed a positive effect on the inactivation rate. Damage to cell membranes, leakage of intracellular compounds, and changes of reactive oxygen species and esterase activity in the spores were detected after inactivation. The results indicated that ozone inactivated fungal spores by firstly destroying cell walls and membranes and then causing the release of intracellular compounds. The fungicidal efficiency of ozone was superior to those of chlorine and chlorine dioxide. In addition, the inactivation efficiency of ozone on fungal spores in real water matrices was reduced to 50.7–91.2% of the efficiency in phosphate buffer. In conclusion, ozone showed high efficiency in the inactivation of fungal spores and could be used as an alternative disinfectant for fungal contamination in drinking water sources.

Surface Dielectric Barrier Discharge plasma: a suitable measure against fungal plant pathogens

Fungal diseases seriously affect agricultural production and the food industry. Crop protection is usually achieved by synthetic fungicides, therefore more sustainable and innovative technologies are increasingly required. The atmospheric pressure low-temperature plasma is a novel suitable measure. We report on the effect of plasma treatment on phytopathogenic fungi causing quantitative and qualitative losses of products both in the field and postharvest. We focus our attention on the in vitro direct inhibitory effect of non-contact Surface Dielectric Barrier Discharge on conidia germination of Botrytis cinerea, Monilinia fructicola, Aspergillus carbonarius and Alternaria alternata. A few minutes of treatment was required to completely inactivate the fungi on an artificial medium. Morphological analysis of spores by Scanning Electron Microscopy suggests that the main mechanism is plasma etching due to Reactive Oxygen Species or UV radiation. Spectroscopic analysis of plasma generated in humid air gives the hint that the rotational temperature of gas should not play a relevant role being very close to room temperature. In vivo experiments on artificially inoculated cherry fruits demonstrated that inactivation of fungal spores by the direct inhibitory effect of plasma extend their shelf life. Pre-treatment of fruits before inoculation improve the resistance to infections maybe by activating defense responses in plant tissues.

Comparison of UV-LEDs and LPUV on inactivation and subsequent reactivation of waterborne fungal spores

Recently, the contamination of fungi in water supply systems has been an area of increasing concern, such as Aspergillus spp. and Penicillium spp. It can cause some waterborne issues such as odor, taste and formation of mycotoxins. Ultraviolet light emitting diodes (UV-LEDs) are considered as a potential alternative to conventional mercury lamps for water disinfection. This study has compared the performance of LPUV (low pressure ultraviolet) and UV-LEDs with emissions at 265, 280 nm and combination emissions at 265/280 nm to test inactivation efficiency, reactivation, viability and electrical energy consumption in the treatment of three water-borne fungal species (Aspergillus niger, Penicillium polonicum, Trichoderma harzianum) at a batch water disinfection system. The results showed that the performances of UV-LEDs were superior for the inactivation of fungal spores compared to the 254 nm (LP), while no statistically differences were observed among the UV-LEDs (p > 0.05). The average photoreactivation rate (k1) of fungal spores irradiated by UV-LEDs and 254 nm (LP) follows the order: T. harzianum > A. niger > P. polonicum. Compared with LPUV, UV-LEDs irradiation at 280 nm and 265/280 nm more efficiently inhibits photoreactivation, which was attributed to that irradiation of 280 nm and 265/280 nm would cause greater membrane damage and increase intracellular reactive oxygen species level of fungal spores according to the flow cytometric results. The electrical energy consumption of UV-LEDs was higher than that of LPUV, which was due to its lower wall plug efficiency. The results of this study can provide additional and beneficial information for the reasonable exploitation of UV-LEDs in water disinfection.

Inactivation of fungi from four typical genera in groundwater using PMS/Cl− system: Efficacy, kinetics and mechanisms

Fungal contamination of drinking water has become a major water quality concern in recent years and as a result, various disinfection methods have been explored. A promising, but relatively unexplored disinfection approach, is the combination of potassium peroxymonosulfate (PMS) with chloride (Cl−) to inactivate fungal spores. In this study, four dominant fungal species found in groundwater were selected to evaluate the efficacy of PMS/Cl− disinfection combination system. The PMS/Cl− system exhibited a delayed Chick-Watson model to inactivate the four genera of fungi and had an improved sustained inactivation effect than a Cl2 system despite the lag period. Four genera of fungi resist PMS/Cl− inactivation to varying degrees: Trichoderma harzianum > Aspergillus niger > Penicillium polonicum > Cladosporium cladosporioides, which was found to be related to their hydrophobicity and individual size. Inactivation of fungi by the PMS/Cl− system was enhanced with increasing PMS or Cl− dosage and also lower pH, but was inhibited by the presence of humic acid (HA). The main reactive species are HOCl/OCl− but not the reactive radicals responsible for the inactivation of fungal spores. The increase of extracellular proteins, total dissolved nitrogen, extracellular adenosine triphosphate and morphologic changes of the four genera of fungi prior to and after inactivation, revealed that the cell membrane of fungal spores was damaged seriously by exposure to the PMS/Cl− system. Moreover, PMS/Cl− system also exhibited a good inactivation effect on the four genera of fungi in real groundwater. Finally, attention should be paid to the use of phosphate buffer solution containing Cl− when PMS is used for disinfection experiments as this could impact results.