Pre-exposure of peracetic acid enhances its subsequent combination with ultraviolet for the inactivation of fungal spores: Efficiency, mechanisms, and implications

Abstract

Waterborne fungi pose a potential threat to water supply safety due to their high resistance to disinfectants. Peracetic acid, as a promising alternative disinfectant to chlorine, has attracted increasing attention in water treatment. In this study, the inactivation of two dominant fungal species (Aspergillus niger and Aspergillus flavus) by sequential application of peracetic acid and ultraviolet (PAA-UV/PAA) was reported for the first time. Results revealed that the pre-exposure of PAA could facilitate the subsequent process of UV/PAA combination and shorten the lag phase in fungi inactivation. After 10 min of PAA pre-exposure, PAA-UV/PAA achieved 3.03 and 2.40 log inactivation of Aspergillus niger and Aspergillus flavus, which were 2- and 4.3-fold higher than that of direct UV/PAA under the same UV and PAA doses. PAA-UV/PAA disinfection also exhibited a stronger regrowth inhibition for incompletely inactivated fungal spores than direct UV/PAA. The increase of pH (5.0–9.0) and humic acid concentration (1.0–5.0 mg L − 1) showed an inhibitory effect on PAA-UV/PAA inactivation, but PAA-UV/PAA was more adaptable in a wide pH range and the presence of humic acid compared to direct UV/PAA. The more severe cell membrane damage and higher reactive oxygen species level in PAA-UV/PAA were evidenced for the first time by flow cytometry. The increased hydroxyl radical generation and higher synergism were primarily responsible for inactivation improvement. This study enhances the further understanding of the PAA-UV/PAA process, and the findings are expected to promote the development of PAA as a promising disinfectant for effective fungi control.