Invitro Study Research Articles

The cytotoxic effect of 4 different wavelengths of Low-level laser therapy on squamous cell carcinoma – An invitro study

Background: Low-level laser therapy is a noninvasive method with the potential ability to change the balance of cell mediators and gene expressions. It affects cellular function resulting in beneficial clinical effects. This study aims to assess the effect of low-level light therapy (LLLT) using four different laser wavelengths on oral carcinoma cell viability in vitro. Methods and Material: HN5 human head and neck squamous cell carcinoma cell lines (HNSCC) were cultured and irradiated using four wavelengths of blue (485 nm), green (532nm), red (660 nm), and near Infra-red (810 nm) in a continuous mode with a dose of 1 J/cm2 (0.1W, 10sec) every 24hours for five consecutive days. Cell viability was assessed by evaluating mitochondrial activity by MTT assay. Results: All the wavelengths resulted in reduced viability of these cells compared to the controls. (P<0.05) There were statistically significant differences in cell viability between different wavelengths (P<0.001). The 810 nm laser irradiation showed the highest percentage of cell survival (55.92%) while 660 nm induced the lowest (36.02%). Conclusion: Different laser wavelengths may result in different effects on irradiated cells and red irradiation showed the lowest cell viability and the infrared laser had the highest cell viability results.

Evalution of shear bond strength of brackets bonded using different hydrophobic and hydrophilic adhesives: An invitro study

A review of the long history of orthodontic bonding adhesives shows that many evolutional developments have occurred from the first chemically-cured composite resins to the most recently introduced light-cured color-change adhesives. An ideal orthodontic adhesive should have adequate bond strength while maintaining unblemished enamel after debonding. Therefore, researchers have been working hard to achieve the best quality and gentlest materials for bonding orthodontic brackets.To evaluate the shear bond strength of brackets bonded using four different adhesives in both dry and in moist environment.A total of 80 orthodontically extracted premolars will be selected. They are divided into 4 equal groups of different adhesives which are further sub-divided into 2 group each (dry and in presence of moisture). All the selected teeth will be cleaned and store in saline until it use. All teeth will be mounted on acrylic block. The shear bond strength will be measured by Lloyd’s Universal testing machine. The obtained data will be statistically evaluated using SPSS22 for Windows using ANOVA test at statistical significance.: The shear bond strength of the four groups were tested with Instron universal testing machine; breaking load at which bond failure occurred was recorded and bond strength was calculated. Data obtained was compiled on MS Office Excel Sheet (2007, Microsoft Redmond Campus, Redmond. Washington, United States.)Data was subjected to statistical package for social sciences (SPSS v 21).Statistically significant difference seen between the hydrophobic and hydrophilic adhesives. In the present study we did Tukey’s post hoc test for pairwise comparison of different hydrophobic and hydrophilic adhesives. It show highly significant values. : The greatest bond strength values were achieved with TransbondXT in both dry and saliva contamination condition. These values were significantly greater than those achieved with Greengloo, RMGIC and Aqualine LC. The overall study conclude that TransbondXT was considered the feasible adhesive material for orthodontic bonding followed by Aqualine LC.

Comparative in-Vitro dissolution study of some sitagliptin generic tablets under biowaiver conditions by UV-Spectroscopy

The objective of this study is to evaluate the bioequivalence of generic sitagliptin tablets from different manufacturers using in-vitro dissolution study under biowaiver conditions through UV Spectroscopy, and compare them with the innovator brand. The dissolution media consisted of three different buffers with varying pH levels, including HCl Buffer pH 2.0, Phosphate Buffer pH 4.0, and Phosphate Buffer pH 7.2. The dissolution process was conducted using a USP type-2 dissolution apparatus with a 900 ml basket. The rotational speed of the paddle was set at 50 RPM, while maintaining a temperature of 37.5°C +/- 2°C. Samples were collected at four different intervals as recommended by the USFDA, which were 15, 30, 45, and 60 minutes. Validation parameters such as Accuracy, Precision, Linearity, LOD, and LOQ were assessed. The dissolution profiles exhibited no significant variability between different brands and within the same brand. Furthermore, the dissolution results of all tablet formulations, including the innovator brand, were analysed using the difference factor (f1) and similarity factor (f2). The findings from this study indicate that both generic brands of sitagliptin tablets meet the USFDA dissolution specifications and can be considered interchangeable.

A scanning electron microscope evaluation of surface roughness of different clear aligners – A comparative in-Vitro study

Clear aligner are thermoplastic polyurethane materials which produces small increments of tooth movements by each aligner tray. Clinical effectiveness of the treatment largely depend on the mechanical properties of the material. The use of scanning electron microscopy (SEM) in invitro environment allows to evaluate the surface morphology of the aligners The aim of the study is to compare the surface roughness of different types of clear aligners after thermoforming and after invitro aging. A sample of 15 aligners from Group A (DURAN), Group B (ERKODUR), Group C (MONOFLEX) were taken for this study. After screening the subjects were scanned by intraoral scanner and STL file were imported to Maestero 3D software. Then resin models were printed and aligners were fabricated in the models by thermoforming machine. The first set of aligners were scanned by Scanning Electron Microscope and another set of aligners were placed in chewing simulator for simulating intraoral environment. Then the second set of aligners were sent for SEM evaluation. Mean comparison of surface roughness and root mean square surface roughness was compared by paired t test and comparison among the groups was done by one way ANOVA test. The surface roughness of both aligners increased after thermoforming. This could be due to thermoforming process which could be due to temperature variation. After the ageing of the aligners in the invitro environment, there is homogenous and smooth layer of the aligner surfaces which could be due to adhesion of biofilm and polishing effect by chewing strokes.

Evaluating the Effect of Adding Calendula officinalis on Anti-microbial Strength of Glass Ionomer Cement: An Invitro Study

Evaluating the Effect of Adding Calendula officinalis on Anti-microbial Strength of Glass Ionomer Cement: An Invitro Study

In-Vitro and In-Silico Studies of Brevifoliol Ester Analogues against Insulin Resistance Condition.

Brevifoliol is a diterpenoid that occurs naturally in the plants of Taxus genus and is widely used as chemotherapy agent for the management of cancer. A series of semisynthetic esters analogues of brevifoliol were prepared by Steglich esterification and attempted for their pharmacological potential against insulin resistance conditions using in-vitro and in-silico assays. The aim of this study is to understand the pharmacological potential of eighteen semisynthetic analogs through Steglich esterification of Brevifoliol against insulin resistance condition Methods: In the in-vitro study, insulin resistance condition was induced in skeletal muscle cells using TNF-α, pro-inflammatory cytokine and these cells were treated with brevifoliol analogues. The most potent analouge was further validated using in-silico docking study against the tumor necrosis factor (TNF-α) (PDB ID: 2AZ5) and Human Insulin Receptor (PDB ID: 1IR3), using the Auto dock Vina v0.8 program. Although, all the analogues of Brevifoliol significantly exhibited the pharmacological potential. Among all, analogue 17 was most potent in reversing the TNF-α induced insulin resistance condition in skeletal muscle cells and also to inhibit the production of TNF-α in LPSinduced inflammation in macrophage cells in a dose-dependent manner. Similarly, in-silico molecular docking studies revealed that analogue 17 possesses a more promising binding affinity than the selected control drug metformin toward the TNF-α and insulin receptor. These findings suggested the suitability of analogue 17 as a drug-like candidate for further investigation toward the management of insulin resistance conditions.

Synthesis and Characterization of Nanosilica-Coated Antibiotics, TAP: A Comprehensive Study Utilizing XRD, EDS, FTIR, SEM, and TEM – Invitro Study

Abstract Aim: To synthesize nanosilica and investigate its properties, focusing on its interaction with antibiotics, particularly triple antibiotic paste (TAP). Various analytical techniques, including X-ray diffraction (XRD), energy-dispersive X-ray spectroscopy (EDS), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and transmission electron microscopy (TEM), were employed. Materials and Methods: The synthesis of nanosilica involved the meticulous combination of ammonia, ethanol, water, and tetraethyl orthosilicate in a sterile conical flask, followed by centrifugation and drying. Nanosilica was further explored by being combined with antibiotics, including doxycycline, Flagyl, and ciprofloxacin, to create novel antibiotic-coated variants. These combinations were subjected to various analytical techniques to assess their properties. Results: The FTIR spectra of silica nanoparticles revealed specific peaks associated with silicon-oxygen bonds and siloxane structures. Nanosilica coated with antibiotics exhibited distinctive peaks indicating the presence of organic components and Si-O-Si bonds, offering insights into their potential applications in drug delivery systems. SEM images displayed uniformly dispersed spherical particles, while TEM images and histograms provided valuable data about the size distribution of nanosilica, essential for applications like drug delivery. XRD analysis confirmed the crystalline nature of silica nanoparticles and offered insights into their crystallographic structure. Conclusion: This comprehensive study showed the synthesis and characterization of nanosilica and its antibiotic-coated variants, with a particular focus on TAP. The findings contribute to our understanding of their structural and compositional properties, providing potential avenues for applications in diverse scientific and technological fields, including drug delivery and nanotechnology.

Comparative Evaluation of Accuracy of Two Apex Locators with Different Irrigants: An In-Vitro Study

Aim: The aim of this in-vitro study will be to evaluate and compare the accuracy of two apex locators in the presence of various irrigants. Methods and Material: The study will be conducted on 80 extracted straight, single-rooted permanent human teeth with mature apices. The teeth will be decoronated. Access cavity preparations will be done and working length will be established with 10 K file or 15 k-file into the canal until its tip emerged through the major apical foramen at ×10 magnification under a stereomicroscope. All the samples selected will be divided into four groups on the basis of irrigants used. Group 1 will be dry without irrigants, used as control, in Group 2 saline will be used as irrigant, in Group 3 sodium hypochlorite will be used as irrigant and in Group 4 chlorehexidine will be used as irrigant. Each group again will be subdivided into two groups on the basis of two different apex locators used (Root ZX Mini J. Morita) and CanalPro (Coltene). All the samples will be embedded in gelatin filled polyurethane bottles to simulate the periodontium. Statistical analysis used: Subsequently, the data will be statistically analysed using one-way analysis of variance (ANOVA) and paired t-test. Results: In this study, group 2 canal pro apex locator is accurate and sodium hypochlorite irrigant shows maximum variation. Conclusions: Canalpro showed accurate values than Root ZX and sodium hypochlorite showed more variations

Assessment of Treatment Outcomes of The Graduate Clinical Program in Suez Canal University Using the American Board of Orthodontics Objective Grading System: An in-Vitro Study

Assessment of Treatment Outcomes of The Graduate Clinical Program in Suez Canal University Using the American Board of Orthodontics Objective Grading System: An in-Vitro Study

Effectiveness of Smear Layer Removal Using Different Activated Irrigating Solutions and Techniques: An in-Vitro Study

effectiveness of Smear Layer Removal Using Different Activated Irrigating Solutions and Techniques: An in-Vitro Study

An In-Vitro Study on the Impact of Light-Emitting Diode (LED) and Laser-Activated Bleaching Techniques on the Color Change of Artificially Stained Teeth at Varying Time Intervals.

Introduction Tooth color is a key factor in the perception of an attractive smile, making bleaching a widely sought-after cosmetic dental procedure. Valued for its minimally invasive approach, this technique effectively lightens teeth to achieve desired aesthetic outcomes. As the desire for immediate results grows among patients, various bleaching agents, and energy sources have been developed to expedite the bleaching process. The study aims to evaluate and compare the efficacy of 35% hydrogen peroxide activated by light-emitting diode (LED) and laser on artificially stained teeth, assessing color changes at different time intervals. Methodology Sixty maxillary central incisors with intact crowns were selected for the study. An artificial staining solution was prepared, and the samples were immersed in it for 15 days to simulate staining. After this period, the teeth were removed from the solution, rinsed with water, and dried. Baseline photographs of each tooth were taken for comparison. The teeth were then divided into two groups, one treated with LED activation and the other with laser activation of 35% hydrogen peroxide as the bleaching agent. The color changes were measured and recorded at different time intervals. Data were presented as mean and standard deviation, with statistical analysis conducted using independent sample t-test and repeated measures ANOVA to compare the efficacy of the two methods. Results Both LED and laser activation of 35% hydrogen peroxide demonstrated significant color changes in the stained teeth. The initial color improvement was noticeable in both groups, with LED and laser treatments producing comparable whitening effects. The mean color change values indicated that both activation sources were effective in enhancing the bleaching process, providing immediate and substantial results. Statistical analysis showed no significant difference between the two methods, suggesting their equivalency in clinical efficacy for tooth bleaching. Conclusion The study concludes that both LED and laser activation of 35% hydrogen peroxide are efficient methods for in-office tooth bleaching, providing immediate and enhanced whitening effects. The comparable results between the two energy sources indicate that can be effectively used to meet the increasing demand for rapid aesthetic improvements in a clinical setting. This finding supports the adoption of both LED and laser bleaching techniques as viable options for dental practitioners aiming to achieve optimal patient satisfaction with minimal invasiveness.

Comparative Analysis of Reverse Torque Values Between Customized CAD-CAM and Prefabricated Abutments and Screws: An In-Vitro Study.

The aim of this study was to examine and compare the mechanical success of customized computer aided design and manufacturing (CAD-CAM) and prefabricated abutments and screws before and after cyclic loading, through measuring removal torque values. The study was performed in accordance with ISO 14801:2016 standarts. Twenty eight dental implants with identical charecteristics were divided into two equal groups. The control group comprised of prefabricated abutments and screws, while the study group included customized CAD-CAM abutments and screws. Removal torque values (RTV) were measured before and after 50.000 and 1 million cyclic loading in both groups. Additionally, to evaluate the screw fatigue performance, they were retightened with 30N torque and RTV measurements were repeated. The data were analyzed using ANOVA, Newman Keuls test and paired t-test. No significant difference was observed in RTVs before cylic loading within each group. However, the RTV's measured before cyclic loading were found to be significantly lower in the study group compared to the control group. Both groups exhibited a significant decrease in RTV after cylic loading. The RTV of the study group was significantly lower than the control group especially after 1 million cycles. No significant difference was found in the screw fatigue performance between two groups. The significantly lower RTVs of customized CAD-CAM abutments and screws before and after cylic loading have raised questions about their effectiveness and widespread use. This study underscores the necessity for further clinical investigations comparing the mechanical success of custom and prefabricated abutments and screws.

Evaluation of Stress Distribution in Implant Retained Fixed Prostheses Fabricated from Polyetheretherketone (PEEK) and Zirconia by CAD/CAM Technology (An in-Vitro Study)

Evaluation of Stress Distribution in Implant Retained Fixed Prostheses Fabricated from Polyetheretherketone (PEEK) and Zirconia by CAD/CAM Technology (An in-Vitro Study)

Effect of Acidulated Phosphate Fluoride Gel on Zirconia Intaglio Surface: An In-Vitro Study

Abstract Aim: To evaluate the micro-shear bond strength (µ-SBS) of resin-modified glass ionomer cement and to assess the chemical and topographical changes in the zirconia fitting surface induced by acidulated phosphate fluoride (APF) gel using scanning electron microscope (SEM) analysis and Fourier transform infrared (FTIR) spectroscopy. Materials and Methods: Thirty-two samples were prepared from two zirconia materials, UPCERA HT White and BruxZir® Solid Zirconia, milled by a computer-aided design/computer-aided manufacturing system. From each zirconia sample, six plates were prepared for FTIR and SEM testing. Following sintering, the samples were divided into control and test groups for each material. The APF gel (1.23%) was applied to the intaglio surface of each test group. To measure the µ-SBS between the zirconia materials and luting cement, 20 rectangular samples of zirconia material were prepared. Ten samples were obtained from Upcera and ten from Bruxzir, with five assigned to the control and five to APF groups. Statistical Analysis: For the µ-SBS test, independent samples t test was conducted to determine the level of significance between the tested groups. Results: FTIR spectroscopy revealed new bands for Upcera and Bruxzir zirconia owing to ion exchange between the formed sodium phosphate and the zirconia surface and the formation of zirconium phosphate by an ester reaction. SEM assessment identified lines, scratches, or surface dissociation that appeared on the intaglio–zirconia surface after conditioning. The µ-SBS test, as indicated by the independent samples t test, showed a significant increase in bond strength of 1.266 and 1.566 MPa for Upcera and Bruxzir zirconia, respectively. Conclusion: This study offers new practical, cost-effective, and accurate tests to enhance the µ-SBS of luting cement to yttria-stabilized tetragonal zirconia polycrystal ceramics.

Pull-Off Forces on Implant-Supported Single Restorations by Sticky Food: An InVitro Study.

To investigate the pull-off forces on implant-supported restorations caused by sticky food, to understand how much retention force cemented restorations must have to withstand chewing forces without unintentional retention loss. The influence of food type, restoration design, and surface treatment were investigated. Monolithic implant-supported CAD/CAM zirconia crowns were fabricated and divided into groups according to their design: no (NC), flat (FC) and high cusps (HC) and subdivided according to surface roughness: rough (r) and smooth (s) (n = 10/group). NC represented the control group. The crowns were fixed in a universal testing machine opposite to each other. Four sticky foods (caramel, fruit jelly, candy strips and licorice) and a resin crown remover were tested. These were heated (36.4°C), placed between the crowns and compressed, then tensile strength tests were performed. The maximum pull-off force was recorded (Newton [N]). Statistical analysis was performed using 3-way ANOVA. The highest mean pull-off force among food samples was with caramel_HC_r (12.09 ± 1.26 N), whereas the lowest was with licorice_FC_s (3.21 ± 0.15 N). For the resin crown remover, a mean pull-off force of 55.41 ± 3.87 N was measured in the HC_r group. Both food type and crown design showed a significant influence on pull-off force (p < 0.001), whereas no significant effect was reported with different surface roughnesses (p = 0.344). This study reported pull-off forces of up to 20 N between all-ceramic restorations caused by sticky food. The clinical implication of these findings is that a cemented implant-restoration must have a minimum retention force of 20 N to withstand unintentional displacement during function.

Time Required for Root Canal Retreatment Using Continuous Rotation, Reciprocation, and Optimum Torque Reverse Motions: An In-Vitro Study.

Objective This study aims to evaluate the time required for canal preparation using three different movement kinematics during retreatment: continuous rotational motion, reciprocating motion, and optimum torque reverse (OTR) motion. Materials and methods The sample comprised 45 single-canal mandibular first premolars. The crowns were sectioned to standardize the root length to 16 mm. The root canals were prepared using the AF Gold mechanical preparation system (25/06). The roots were obturated using the lateral condensation technique and kept at 100% humidity at 37°C for seven days. The sample was randomly divided based on the movement pattern used during retreatment into three groups (N = 15): group 1 is continuous rotational motion using the ProTaper Universal Retreatment system; group 2 is reciprocating motion using the WaveOne Gold system; and group 3 is OTR motion using the ProTaper Universal Retreatment system. The retreatment time was measured in seconds by summing two times: T1 (time to reach the apex) and T2 (time to achieve adequate cleaning). The data were statistically analyzed using a one-way ANOVA with a significance level of α = 0.05. Results The results showed that the time required for canal preparation during retreatment with WaveOne Gold files using reciprocating motion was significantly longer than the time needed with ProTaper Universal Retreatment files using continuous rotary motion or OTR motion (P < 0.05). Conclusions The use of OTR motion did not affect the canal preparation time when used with files designed for continuous rotary motion retreatment. The use of WaveOne Gold files for canal preparation during retreatment was associated with a longer working time than ProTaper Universal Retreatment files.

Impact of recycling on efficiency and efficacy of copper nickel titanium wire and heat activated nickel titanium wire – An invitro study

The aim of the study is to assess the feasibility of reusing copper nickel titanium wire and heat activated nickel titanium wires by evaluating the changes in efficiency and efficacy of wire after different sterilization techniques. 120 total samples were taken. 60 samples of Damon copper nickel titanium (ORMCOTM) and 60 samples of heat activated nickel titanium wires (ORMCOTM). Damon CuNiTi and HANT were divided in 4 different sub groups of 15 samples each. 15 as received wires served as controlled group. The remaining wires were placed intraorally for a period of 4 weeks in patients undergoing orthodontic treatment. After 4 weeks, these wires were taken out. Remaining 45 wires were divided on the basis of sterilization method to be used. (autoclave, 70% isopropyl alcohol, 2% glutaraldehyde solution). Maximum load and tensile strength were tested using universal testing machine. No significant difference in mechanical properties of wires after various sterilization process (p value &amp;#62; 0.05). There no such difference in mechanical properties of controlled group wires and wires those are placed intraorally for 4 weeks. Results suggested that there was no statistically significant difference in the Ultimate Tensile Strength and Maximum Load of Damon Copper Nickel-Titanium wire and Heat Activated Nickel Titanium wire following various sterilization procedures. These wires can be reused after sterilization.

Isothiocyanates attenuate heparin-induced proliferation of colon cancer cells invitro.

Isothiocyanates (ITCs), prevalent in cruciferous vegetables, are known for their anticarcinogenic properties. Prior research has indicated that heparin can stimulate the growth of colon cancer cells. However, the implications of ITCs in the diet of cancer patients receiving heparin-based therapies have yet to be fully understood. This exploratory invitro study examines the proliferative effects of low-molecular-weight heparin (LMWH) on human colon cancer cells and assesses the antiproliferative potential of four ITC compounds, exploring possible epidermal growth factor family of receptor tyrosine kinases (Erb-B) related mechanisms. We evaluated cell viability in HCT-116 and HT-29 cell lines following treatment with ITCs alone or combined with LMWH (20 μg/mL) at various concentrations (1-100 μM). Clonogenic and wound-healing assays were performed after 24 h of treatment with 5 μM ITCs. Additionally, messenger RNA (mRNA) and protein expression of Erb-B family genes was measured using quantitative polymerase chain reaction (qPCR) and Western blotting. Statistical analysis was conducted using analysis of variance (ANOVA) with Dunnett's post hoc test. Results indicated that the half-maximal inhibitory concentration (IC50) values for Phenylethyl isothiocyanate (PEITC), Benzyl isothiocyanate (BITC), and Sulforaphane (SFN) were lower than those of Allyl isothiocyanate (AITC) in LMWH-stimulated HCT-116 (20.77, 19.10, and 44.05 μM, respectively) and HT-29 (74.94, 26.77, and 43.49 μM, respectively). PEITC and SFN significantly reduced ErbB1 (epidermal growth factor receptor (EGFR)) and ErbB4 (receptor tyrosine-protein kinase erbB-4) expression, while BITC decreased ErbB2 (receptor tyrosine-protein kinase erbB-2) and transforming growth factor beta (TGF-β) expression in HCT-116 cells (all, p < .05). PEITC, BITC, and SFN also increased proapoptotic Bax expression and decreased the antiapoptotic B-cell lymphoma 2 (Bcl-2) expression (all, p < .05). These findings suggest that specific ITCs may mitigate cancer cell proliferation induced by LMWH in cancer therapies, highlighting their potential therapeutic efficacy.

The Gloss Retention of Esthetic Restorations Following Simulated Brushing with Charcoal Oral Products: An In-Vitro Study.

To assess the gloss and gloss retention of two esthetic restorative materials after brushing with a charcoal-infused toothbrush and activated charcoal toothpaste compared to their standard counterparts in all possible combinations. In this study,104 disc-shaped specimens were prepared, 52 from each of the two materials: Beautifil II LS composite resin (Giomer) and Fuji II LC resin-modified glass ionomer (RMGI). Specimens of each material were divided into 4 groups (n = 13) according to the tested brushing procedure: group A-standard toothbrush + standard non-whitening toothpaste (S-S), group B-standard toothbrush + activated charcoal toothpaste (S-CH), group C-charcoal-infused toothbrush + standard non-whitening toothpaste (CH-S), and group D-charcoal-infused toothbrush + activated-charcoal toothpaste (CH-CH). Gloss was recorded using a gloss meter initially and after each brushing protocol. The data were statistically analyzed with two-way analysis of variance (ANOVA) and Tukey HSD post hoc tests using SPSS® v.27 software at 5% significance level. Beautifil II LS showed the highest gloss values after brushing in group D (44 ± 3.9), which was not significantly different from its initial value (46.1 ± 1.8), followed by groups A (32.2 ± 4), B (23.2 ± 3.6), and C (22.7 ± 3.9), while Fuji II LC showed its highest gloss values after brushing in groups D (16.6 ± 3.7) and a (15.4 ± 8.7), followed by groups C (10.9 ± 6) and B (4.4 ± 2.5), all were significantly lower than their respective initial gloss values. The gloss of Giomer and RMGI reduced significantly following the brushing with the tested brushing procedures except for the Giomer group brushed with a combination of charcoal-infused toothbrush and activated-charcoal toothpaste. The brushing using a combination of charcoal-infused toothbrush and activated-charcoal toothpaste is recommended for the gloss retention of Beautifil II LS restorations. However, the gloss of the Fuji II LC cannot be retained after any of the brushing procedures. How to cite this article: Farghal NS, Awadalkreem F, Abouelhonoud NA, et al. The Gloss Retention of Esthetic Restorations Following Simulated Brushing with Charcoal Oral Products: An In-Vitro Study. J Contemp Dent Pract 2024;25(5):473-479.

Reliability of sealer penetration measurement methods and assessment of dentine penetrability after endodontic retreatment: A correlation invitro study.

This study evaluated the reliability of sealer penetration measurement methods used with confocal laser scanning microscopy in correlation with the percentage of residual root filling and examined the effect of residual root-filling material on dentine penetrability after retreatment. Extracted teeth were randomly divided into different groups according to the obturation sealer used (n = 6); BioRoot RCS; MTA Fillapex; Bio-C and AH Plus. Root-filling material was removed before the secondary chemo-mechanical preparation and obturation using fluorescein labelled AH Plus. Multiple micro-computed tomography scans were obtained followed by confocal laser scanning microscopy to measure the penetration of the labelled sealer into the dentinal tubules using four different methods. Measuring sealer penetration into radicular dentine using the penetration percentage method was found to be the most reliable. Dentine penetrability during retreatment did not seem to be affected by the type of residual root-filling material.