Invitro Study Research Articles

Evaluation of anti-acne activity of human whole saliva against acne vulgaris: An invitro study.

Acne vulgaris (AV) is a multifactorial inflammatory skin disorder, affecting 9.45% of the world's population. AV can be painful, discomforting, and disfiguring due to scarring, leading to physiological distress, and economic burden. AV pathogenies can be due to various factors, key ones include follicular plugging, colonization by the microorganism, endocrinological factors, and inflammation. Gram-positive bacteria Cutibacterium acnes and Staphylococcus epidermidis are the most common pathogens isolated from patients with AV. The present study aimed to investigate the anti-acne activity of whole human saliva against bacteria that cause AV. The saliva sample from four different individuals was collected at three different intervals. These samples were used to perform antimicrobial susceptibility test (AST) against the pathogens. Kirby-Bauer disk diffusion susceptibility test, and Broth dilution method using Resazurin were performed. The human saliva was effective in inhibiting Cutibacterium acnes and Staphylococcus epidermidis those causing AV. The Minimum inhibitory activity and disc diffusion assay depicted potency of saliva. The pH of the saliva samples was found to be more acidic in the morning samples when compared with the evening samples. The afternoon sample was found to be more effective when compared with the other two intervals. Saliva shows effective anti-acne activity by inhibiting the growth and proliferation of acne-causing bacteria.

Formation of bone tissue apatite on starch-based nanofiber-capped nanohydroxyapatite and reduced graphene oxide: a preliminary study.

In the present study, blends of polyvinyl alcohol (PVA), starch (SH), nanohydroxyapatite (Nano-HA), and reduced graphene oxide (r-GO) were used to fabricate an electrospun nano scaffold (ENS), via electrospinning for their potential application in oral and maxillofacial bone soft and hard tissue regeneration. The scaffold was characterized for its physicochemical and mechanical properties. An invitro study was carried out using human osteoblast MG-63 bone cells. Surface characterization, particularly the analysis of calcium content, was performed before and after immersion in the simulated body fluid (SBF). Additionally, the impact of surface treatment on antimicrobial activity was investigated. The results demonstrated that the tensile strength (18.12 ± 0.14MPa), elongation at break (19.23 ± 0.11%), and flexing index (20.15 ± 0.13%) of the ENS were outstanding, indicating promising performance. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assays demonstrated the biocompatible nature of the ENS. The bioactivity test result of ENS showed excellent deposition of bone apatite crystals. The ENS exhibited antimicrobial properties against E. coli (3.41 ± 0.03mm) and S. aureus (3.12 ± 0.08mm). The ENS, possessing the desired properties, has the potential to be tested in large animals for oral and maxillofacial bone and soft tissue regeneration after obtaining the necessary approvals. The developed ENS offers a promising solution for bone tissue regeneration in the oral and maxillofacial region.

The Effect of Implantoplasty on Fracture Resistance and Implant Surface Changes: An InVitro and Finite Element Analysis Study.

Implantoplasty can be performed on implants diagnosed with peri-implantitis to facilitate implant decontamination and improve access for oral home care. However, its effect on the mechanical strength of the implant is still uncertain. This study aimed to evaluate the effect of implantoplasty on the fracture resistance of dental implants with various degrees of bone loss, as well as its surface changes. Eighty 4.2 × 13 mm conical connection dental implants were allocated evenly into four groups based on the bone defect morphology: circumferential or semi-circumferential, and 3 or 5 mm vertical height. Half of the implants underwent implantoplasty with tungsten carbide finishing burs. Weight, volume, and surface roughness of the implants were recorded prior to and after instrumentation. All implants were subjected to static loading to failure or fracture and the implant surfaces were then analyzed using optical microscopy. Finite element analysis was carried out to assess the stress pattern on dental implants after implantoplasty. Implantoplasty significantly reduced the fracture resistance of implants with all defect morphologies, aside from those with 3 mm of circumferential bone loss. Implants with 5 mm of peri-implant bone loss also experienced significantly reduced fracture resistance compared to the 3 mm group. Significant decrease in fracture resistance was only observed between the circumferential and semi-circumferential groups with 5 mm of bone loss. Surface roughness was also significantly reduced following implantoplasty. The results from finite element analysis revealed a change in pattern of stress concentration in the implant after implantoplasty. Implantoplasty negatively impacted the fracture resistance of standard diameter dental implants in most scenarios. The increase in exposed implant length resulted in a decrease in fracture resistance. This increase in fracture risk should be considered prior to implantoplasty, especially in implants with more advanced bone loss.

The activation of inflammatory responses in the brain is potentiated by over-expressing acetylcholinesterase in myeloid lineage of transgenic mice.

Acetylcholinesterase (AChE) has functions in neuroinflammation, beyond its classical role in neurotransmission. Understanding the role of AChE in neuroinflammation is of great significance, as it highlights the potential therapeutic targets for the treatment of neurodegenerative diseases. In an invitro study, the expression of AChE was up-regulated in lipopolysaccharide (LPS)-induced microglia/macrophage and contrarily potentiated the inflammatory responses via disturbing the cholinergic anti-inflammatory pathway (CAP). However, the regulation of AChE in neuroinflammation has not been revealed invivo yet. Here, we aim to uncover the inflammatory roles of microglial AChE in LPS-induced neuroinflammation by using the conditional AChE over-expression mouse model. AChE was specifically over-expressed in the myeloid cell linkage of mouse by applying CRISPR/cas9 combined with Cre-LoxP system. LPS was intraperitoneally injected into the mice to induce inflammation. The results showed that the inflammation, induced by LPS, was aggravated in the brain of transgenic mice having over-expression of AChE in microglia. The expressions of pro-inflammatory cytokines were robustly up-regulated in the brains of LPS-treated transgenic mice, as compared to the LPS-treated wildtypes. In parallel, the activations of microglia and astrocytes in hippocampus were enhanced significantly in AChE transgenic mice. Transcriptomics analysis further confirmed the severer inflammation in the transgenic mice than the wildtype after LPS administration. These findings shed light on the regulation of microglial AChE in neuroinflammation invivo for the first time, presenting another angle to understand the role of AChE in neurodegenerative diseases.

Does the Transillumination Technique Using a Diagnostic White Tip Influence the Degree of Conversion of the Infiltrant Resin? A Case Report With InVitro Insights: A New Technique to Improve the Predictability During the Application of Resin Infiltration: Transillumination Using a Diagnostic White Tip.

This study aimed to evaluate, through a case report combined with invitro study, whether a new diagnostic white tip for the transillumination technique during resin infiltration influences the procedure and degree of conversion (DC). A clinical case report demonstrated resin infiltration using the transillumination technique with a new white diagnostic tip (Radii Xpert LED diagnostic tip, SDI, Bayswater, VIC, AU) along with a light-curing device to enhance aesthetic results. An invitro study assessed the DC of two diagnostic white tips (Radii Xpert LED diagnostic tip [SDI, Bayswater, VIC, AU] and Valo Grand White Light Lens [Ultradent Prod., South Jordan, UT, USA]) compared to that achieved using conventional tips of both light-curing devices. Statistical analysis was performed using two-way ANOVA and Tukey's test (α = 0.05). The clinical case achieved complete masking of the white spot lesion. Invitro, the diagnostic white tips exhibited significantly lower DC values compared to conventional tips (p = 0.001), regardless of the light-curing unit. The diagnostic white tip used in the transillumination technique improves resin infiltration visibility without compromising the degree of conversion. The transillumination technique with the diagnostic white tip enables monitoring of resin infiltration while maintaining effective polymerization.

Can ultrasonography be used to detect root perforation? An invitro study.

This study assessed the usability of ultrasonography in detecting root canal perforations. The buccal side of incisor root were perforated. The actual lengths up to the perforation site were measured by visualisation of the tip of a file. Specimens were embedded in gypsum, and it was scraped to simulate bone resorption. Resorption cavities were filled with ultrasound gel. Measurements were taken by advancing a file through the canal until the tip became visible in the ultrasound image. 3D scans were obtained using cone-beam computed tomography (CBCT) and the distance between the coronal point of the resorption cavity and the reference point was measured. The actual distance of the resorption cavity from the reference point varied 6-10.6 mm. The same distances were measured via ultrasound as 5.7-10.9 mm, while measured using CBCT 6-10.5 mm. No significant differences were found. Ultrasonography shows promise for detecting root perforations, however further research is needed.

Evaluation of the Mechanical Properties of Provisional 3DPrinted Resin After Repair with Different Materials: An In-Vitro Study.

To evaluate the mechanical properties of the 3D printed provisional restoration material that was repaired using different materials. The bar specimens have been manufactured using three-dimensional printing technology in accordance with the ISO 10477:2020 standards and divided into 5 groups randomly. For repair material application and replacement on the standardized silicone mold, the test specimens were ground at the center by 1x2x2 mm. No grinding was done on the control group specimens. Flowable composite, bis-acrylic composite resin, polymethyl methacrylate resin, and temporary 3D printing resin are utilized as repair materials (n=16). The specimens underwent a three-point-bending (3PB) test, with a cross-head speed of 1mm/min, in order to assess their flexural strength (FS) and flexural modulus (FM). The data received statistical analysis with one-way ANOVA and Tukey test. A Weibull analysis was performed, and the Weibull modulus of specimens was calculated. Control group specimens were showed the highest FS (142±12.6 MPa) and FM (4497±1205 MPa) values. Among the test groups, the utilization of temporary 3D printing resin as a repair material exhibited the greatest FS (67±33.3 MPa) values and showed statistical significance when compared to all other groups. Repairing 3D-printed provisional resin material weakens its mechanical properties. However, utilizing the own resin made of 3D-printed provisional resin material can be an effective choice for implementing minor modifications and additions.

A comparative invitro study of distinct and novel stent geometries on mechanical performances of poly-L-lactic acid cardiovascular stents.

Poly-L-lactic acid (PLLA) is one of the representative polymeric materials serving as bioresorbable stents (BRS) for cardiovascular disease due to its proper biodegradation, high biocompatibility, and adequate mechanical properties among polymer candidates for BRS. However, PLLA BRS as cardiovascular stents also have limitations because their mechanical properties including low radial strength and high elastic recoil are inferior to those of metallic-based BRS stents. In the study, we developed and manufactured distinct and novel types of stent geometries for investigating mechanical properties of thin-walled PLLA BRS (110 μm) for cardiovascular applications. Five key mechanical tests, including radial strength, crimping profile, flexibility, elastic recoil, and foreshortening were performed through a comprehensive analysis. In addition, we applied the finite element method for further validation and insight of mechanical behaviors of the PLLA BRS. Results revealed that Model 2 had advantages in high flexibility as well as radial strengths, which would be a proper option for complex and acutely curved lesions. Model 3 would be an optimum selection for stent placement in mild target site due to its strength in minimum elastic recoil. Even though Model 4 showed the highest radial strength, finite element simulation showed that the geometry caused higher maximum stress than that of Model 2 and Model 3 during the crimping process. Model 1 showed the most vulnerable geometry among the tested models in both invitro and finite element analysis. Such data may suggest potential guidance in regard to understanding the mechanical behaviors of PLLA BRS as not only applicable cardiovascular but also peripheral and intracranial stents.

Accuracy of Zygomatic Implant Placement Using Task-Autonomous Robotic System or Dynamic Navigation: An InVitro Study.

To evaluate and compare the accuracy of task-autonomous robot-assisted implant surgery (RAIS) and dynamic computer-assisted implant surgery (dCAIS) for zygomatic implant placement. Ten atrophic edentulous maxilla models requiring zygomatic implant (ZI) placement were randomly divided into the RAIS and dCAIS groups. Osteotomies and implant placement were performed under the guidance of a task-autonomous robotic system or dynamic navigation system. A total of 20 ZIs were analyzed. The angular, coronal, lateral coronal, coronal depth, apical, lateral apical, and apical depth deviations were measured and analyzed between the two groups. The primary outcome parameters were the angular deviations between the planned and the placed ZIs. Data was subjected to descriptive and comparative statistical analysis. The significance of inter-group differences for continuous variables was assessed with Student's two-sample t-tests, Welch two-sample t-tests, and Mann-Whitney U tests according to the distribution normality and variance homogeneity. ZI placement deviations were compared between the RAIS and dCAIS groups, showing a mean angular deviation of 0.92 ± 0.40° versus 2.03 ± 0.53° (p < 0.001), a mean (±SD) coronal deviation of 0.48 ± 0.25 mm versus 1.29 ± 0.46 mm (p < 0.001), and a mean apical deviation of 0.88 ± 0.28 mm versus 1.96 ± 0.46 mm (p < 0.001). For computer-guided ZI placement, task-autonomous RAIS was superior to dCAIS in terms of accuracy.

The Effect of Intraoral Thermal Changes on the Mechanical Behavior of Nickel-Titanium Wires: An In-Vitro Study.

Background The continuous light force with a wide range of activation describes the excellent properties of nickel-titanium (NiTi) archwires. Shape memory is mainly affected by intraoral thermal changes. This study evaluated the effect of three different constant temperatures (i.e., 12°C, 37°C, and 50°C) on the unloading value of three different 0.016 × 0.022 NiTi archwires. Methodology Three types of 0.016 × 0.022-inch diameter NiTi archwires (American Orthodontics®, Sheboygan, Wisconsin, USA) were used. These were the superelastic type (NT3-SE®), the heat-activated type at 25°C (Thermal Ti-D®), and the thermally activated type at 35°C (Thermal Ti-Lite®). The unloading forces of the wires were evaluated using a classic three-point bending test (a universal testing machine: Testometric 350M®, Instron, Lincoln Close, Rochdale, England) under three different constant temperatures (12°C, 37°C, and 50°C). Results All types of wires showed thermal sensitivity; at higher temperatures, the unloading forces increased differentially between small and large deflections, while at lower temperatures, the residual strain increased for all wire types. The most affected type by the thermal changes was thermal Ti-Lite®, followed by thermal Ti-D®, and the superelastic type NT3-SE® showed a behavior similar to thermal wires. At the low temperature (12°C), all wire types showed an incomplete load/deflection curve, whereas no value was measured at unloading points 2, 1, and 0.5 mm. At the normal temperature (37°C), NT3-SE® type and thermal Ti-D® were similar in force level, while significant differences were noted between both previous types and Thermal Ti-Lite®. At the high temperature (50°C), all wire types showed a higher force level, while significant differences between the wire types were inconsistent. In contrast, increasing the temperature from 37°C to 50°C increased the force levels between 40% and 84% for NT3-SE®, between 44% and 64% for the thermal Ti-D®, and between 61% and 268% for the Thermal Ti-Lite®. When comparing the force levels between 12°C and 50°C at 3 mm, the force levels increased by 66% for NT3-SE®, 25% for Thermal Ti-D®, and 109% for thermal Ti-Lite®, while on comparing the forces between 12°C to 37°C, the forces increased between 15% and 95% for NT3-SE®, 20% and 88% for thermal Ti-D®, and 26% and 78% for thermal Ti-Lite®. The value of residual strain was greater at low temperatures and smaller at higher temperatures, while no significant differences were detected between 37°C and 50°C. Conclusions The temperature degree deeply affected the mechanical behavior of all test NiTi wires; the superelastic type behaved similarly to thermal wires. Increasing the temperature degree leads to more unloading forces and less residual strain.

Evaluation of the Conventional Acid-Etching System and the Self-Etching Primer in Bonding Metallic Orthodontic Brackets: An In-Vitro and In-Vivo Study.

This study aimed to compare the shear bond strength (SBS) of enamel-bonded orthodontic brackets with the conventional acid etching (CAE) system and the self-etching primer (SEP)in vitro and to compare the clinical performance of both systems when used in the treatment of malocclusion patients. In the first part of the study, 40 extracted human premolars were randomly divided into two groups containing 20 teeth. The first group (the conventional enamel etching group) employed37% phosphoric acid before bonding the metallic brackets (0.022-inch slot, MBT prescription, American Orthodontics, Sheboygan, WI, USA). The etching system wasTetric 5th (Ivoclar Vivadent, Schaan, Liechtenstein). The second group used a SEP (Sep Tetric 7th, Ivoclar Vivadent, Schaan, Liechtenstein) to bond the same brackets. In the first part of the study, SBS was evaluated, followed by the adhesive remnant index (ARI) assessment. The second part of the study (i.e., the clinical part) assessed a cohort of 30 patients during a 6-month observation period. The upper 10 teeth (from the second premolar on the right side to the second on the right side) were bonded using the chosen method for each patient in the clinical assessment. That is, 150 teeth in each group were evaluated regarding the failure rate. The ARI was assessed for those teeth that lost their brackets. The mean SBS was greater in the SEP group compared to the CAE group (17.93 MPa and 16.60 MPa, respectively; P = 0.014). The difference was not statistically significant. Conversely, the failure rate was lower in the CAE group compared to the SEP group, with a failure rate of 6% and 14.7%, respectively. The difference was statistically significant (P = 0.014). However, the ARI showed no statistically significant difference in in-vivo and in-vitro analyses, as most bracket failures were at the adhesive level. Laboratory results showed no statistical difference in the SBS mean values between the two groups. Clinically, the SEP group showed a greater failure rate than the CAE group, but both failure rates in the two groups were within the clinically acceptable range. The ARI did not show any difference between the two groups in terms of the failure site when the evaluation was conducted in vivo and in vitro, as most of the areas of failure were concentrated in the material itself.

Influence of Material, Sterilization, and Disinfection on the Accuracy of Three-Dimensional Printed Surgical Templates: An InVitro Study.

To evaluate the influence of the three-dimensional (3D) printing technology, material, sterilization, and disinfection on the accuracy of guided surgical templates. Fifty printed resin surgical templates were designed and fabricated using a digital light processing 3D printer with a photopolymerizing resin, and 50 printed metal surgical templates were designed and fabricated using a selective laser melting 3D printer with a titanium alloy. Templates from both groups were randomly divided into five subgroups involving different sterilization and disinfection procedures. The group without any sterilization or disinfection procedure served as the control group, whereas the other groups were used as the study groups (hydrogen peroxide gas plasma sterilization, 5% povidone-iodine disinfection, 75% ethyl alcohol disinfection, and steam autoclave sterilization). Implant simulations were performed on the 3D-printed resin models, and postoperative impressions were acquired with scan bodies attached to the implants. All surgical templates were digitally scanned. The root mean square was used to determine and quantify fabrication accuracy and reproducibility, and the definitive and planned implant positions were compared. The printed resin templates exhibited lower fabrication accuracy and reproducibility, as well as higher 3D deviations, after steam autoclave sterilization (p < 0.001); however, the printed metal templates were not affected by the different sterilization or disinfection procedures (p > 0.05). Printed metal surgical templates are viable alternatives for guided implant surgery. Preoperative steam or gas plasma sterilization is recommended, especially for metal templates, as resin templates show deformation and decreased accuracy after steam sterilization. chictr.org.cn number: ChiCTR2400081334.

Evaluation of Reliability and Validity of Different Thicknesses of Occlusal Contact Registration Strips Vs Conventionally used Articulating Papers – An Invitro Study

Aim: To compare and evaluate the reliability and validity of different thicknesses of Occlusal Contact Registration Strips (OCRS) under simulated occlusal load.  Settings and Design: In-Vitro Comparative Study  Materials and Methods: Articulated epoxy resin dental models obtained from completely dentulous patients were interposed with occlusal contact registration strip of various thicknesses and subjected to constant axial compressive load using universal testing machine. The photographs of consistent registration marks were subjectively assessed using a computer software.  Statistical Analysis Used: Analysis of Variance (ANOVA) and post hoc mean multiple comparison using Dunnett T3 test.  Results: The thinnest occlusal registration strip used in this study registered the highest average number of markings with a borderline statistically significant difference (P=0.06). The highest average area of markings was registered by the thickest strip, which had a near marginal significance (P=0.09), whereas the lowest average area was produced by the thinnest strip which was statistically significant (P=0.03).  Conclusion: There exists a relationship between the thickness of an occlusal contact registration strip, the number and the area of the marks registered. The average number of marks registered was inversely proportional to the thickness of the occlusal indicator. Hence, the thinner the occlusal contact registration strip, the more reliable is the occlusal contact registration. The average area of occlusal contact registration mark varies proportionately to its thickness. Hence, the thickest occlusal contact registration strips were more valid for marking occlusal contacts.

Passive fit and time efficiency for prefabricated versus conventionally constructed cobalt chromium CAD\\CAM 3-unit implant supported frameworks in free end saddle models: a pilot invitro study

BackgroundThe passive fit of 3-unit implant supported prefabricated metal screw-retained prosthesis before implant placement might be difficult. Hence, we aim to evaluate the passive fit and time efficiency of CAD/CAM 3-unit implant supported fixed prostheses that were constructed based on virtual versus those based on actual implant positions in Kennedy Class I models.MethodsA sample of 5 Kennedy class I models with thin wiry ridges were restored by 20 frameworks bilaterally, 10 based on actual (group A) and 10 based on virtual (group V) implant positions. The models were imaged using cone beam computed tomography and scanned using an intraoral scanner. The STL (Standard Tessellation Language files) and the DICOM (Digital Imaging and Communications in Medicine) files were registered on a 3D planning software. A CAD/CAM surgical guide was planned, resin printed and used for installing 6 implants bilaterally. In group V, the framework was designed based on the virtual scan bodies and virtual multi-unit abutments, while in group A intra-oral scanning of the model after attaching the scan bodies was necessary. Frameworks of both groups were milled and tested for passive fit using 8 clinical tests. McNemar and Wilcoxon signed rank tests were used to study the effect of the group on passive fit and time efficiency, respectively. The significance level was set at P ≤ 0.05.ResultsNo statistically significant difference was found between group V and group A frameworks regarding passive fit (p-value = 1, OR = 0.5) and time efficiency (P = 0.179, Effect size = 0.948).ConclusionWithin the limitations of this study, it can be concluded that in free end saddle cases, prefabricated CAD\\CAM 3-unit implant-supported cobalt chromium screw retained prostheses can achieve an adequate passive fit. However, their fit might be negatively affected in thin ridges and they might require some adjustments.

The influence of hydrogen sulfide on gingival wound healing: An invitro study.

Flowchart and timeline (in hours) of the invitro experimental procedures.

Comparison of Different Obturation Techniques in the Dentinal Tubule Penetration of EndoSequence® Bioceramic Sealer HiFlow™: An In-Vitro Study.

Background This study aimed to assess the dentinal tubule penetration of bioceramic sealer(DTPB) using EndoSequence® Bioceramic Sealer HiFlow™ (BCHF). Three obturation techniques were compared, namely, single cone (SC), cold lateral condensation (CLC), and warm vertical compaction (WVC) using scanning electron microscopy (SEM). Methodology A total of 45 intact mandibular premolars were decoronated, instrumented, and irrigated with uniform procedures. Subsequently, they were randomly assigned into the following three groups according to the gutta-percha and BCHF obturation technique: Group 1 (n = 15) with the SC technique, Group 2 (n = 15) with the CLC technique, and Group 3 (n = 15) with the WVC technique. Afterward, the roots were sectioned at three levels (coronal, middle, apical) from the apex and examined using SEM to determine the maximum distance of DTPB in µm. The collected data were analyzed using SPSS software (IBM Corp., Armonk, NY, USA) using the one-way analysis of variance test and Bonferroni tests. The level of significance was set at α of 0.05. Results Significant differences were observed in the maximum distances of DTPB values among the three levels across groups (p < 0.05). Both the WVC and CLC groups exhibited higher values compared to the SC group (p < 0.05). Specifically, the DTPB maximum distance at the apical third was significantly higher in the CLC group than in the WVC and SC groups (p < 0.05). Additionally, the coronal third in each group demonstrated significant DTPB maximum distance (p < 0.05) compared to the other groups. Conclusions BCHF with the WVC and CLC obturation technique showed the best performance in DTPB maximum distance values at the three levels.

Exploring the reduction in aquaporin-4 and increased expression of ciliary neurotrophic factor with the frontal-striatal gliosis induced by chronic high-fat dietary stress.

High-fat diet (HFD)-induced obesity induces peripheral inflammation and hypothalamic pathogenesis linking the activation of astrocytes and microglia. Clinical evidence indicates a positive correlation between obesity and psychiatric disorders, such as depression. The connectivity of the frontal-striatal (FS) circuit, involving the caudate putamen (CPu) and anterior cingulate cortex (ACC) within the prefrontal cortex (PFC), is known for its role in stress-induced depression. Thus, there is a need for a thorough investigation into whether chronic obesity-induced gliosis, characterized by the activation of astrocytes and microglia, in these brain regions of individuals with chronic obesity. The results revealed increased S100β+ astrocytes and Iba1+ microglia in the CPu and ACC of male obese mice, along with immune cell accumulation in meningeal lymphatic drainage. Activated GFAP+ astrocytes and Iba1+ microglia were observed in the corpus callosum of obese mice. Gliosis in the CPu and ACC was linked to elevated cleaved caspase-3 levels, indicating potential neural cell death by chronic HFD feeding. There was a loss of myelin and adenomatous polyposis coli (APC)+ oligodendrocytes (OLs) in the corpus callosum, an area known to be linked with injury to the CPu. Additionally, reduced levels of aquaporin-4 (AQP4), a protein associated within the glymphatic systems, were noted in the CPu and ACC, while ciliary neurotrophic factor (CNTF) gene expression was upregulated in these brain regions of obese mice. The invitro study revealed thathigh-dose CNTF causing a trend of reduced astrocytic AQP4 expression, but it significantly impaired OL maturation. This pathological evidence highlights that prolonged HFD consumption induces persistent FS gliosis and demyelination in the corpus callosum. An elevated level of CNTF appears to act as a potential regulator, leading to AQP4 downregulation in the FS areas and demyelination in the corpus callosum. This cascade of events might contribute to neural cell damage within these regions and disrupt the glymphatic flow.

Tri-Lineage Differentiation Potential of Stem Cells from Human Exfoliated Deciduous Teeth - An In-Vitro Study

ABSTRACT Background: The use of SHED might bring advantages for tissue engineering over the use of stem cells from adult human teeth as SHEDs were reported to have higher proliferation rate and increase cell population doublings as compared with stem cells from permanent teeth. Objective: The study’s objective was to assess the growth kinetics and tri-lineage differentiation capacity of stem cells derived from human exfoliated deciduous teeth. Material and Method: After being propagated through multiple sequential subcultures, adherent fibroblastic cells in the pulp tissue culture from human exfoliation teeth were evaluated to assess their tripotent differentiation potential into bone, cartilage, and adipose cell lineages. Result: The stem cells derived from dental pulp had a comparatively high ability for proliferation and successfully differentiated into chondrocytes and osteocytes while less significant for adipocytes. Conclusion: Stem cells from Human Exfoliated Deciduous teeth are mesenchymal stem cells which are multipotent and can serve as a promising incentive for therapeutic and future research purposes.

MicroRNA-145 Inhibitor Induced Proliferation of Human Oral Squamous Cell Carcinoma by Upregulating C-Myc and Downregulating Caspase-3 Genes: An In-Vitro Study

MicroRNA-145 Inhibitor Induced Proliferation of Human Oral Squamous Cell Carcinoma by Upregulating C-Myc and Downregulating Caspase-3 Genes: An In-Vitro Study

Cleaning Ability of Three Different Rotary Files Systems; (An In-Vitro Study)

Cleaning Ability of Three Different Rotary Files Systems; (An In-Vitro Study)