Beta-glucan is currently under consideration as an alternative to in-feed antibiotics. The aim of the study was to investigate Agrobacterium sp. ZX09 beta-glucan on intestinal morphology, cytokine concentration, mucin expression and microbial populations of weaning piglets. Pigs were randomly assigned to one of five dietary treatments supplemented with 0, 25, 50, 100 and 200mg/kg beta-glucan. Data showed an increase in ADG at the 100mg/kg group (p=.03). A significant increase in villus height and reduction in crypt depth were fund in ileal tissue at the 100mg/kg inclusion level (p<.05). Dietary supplementation of 100mg/kg beta-glucan enhanced IL-10 concentration (p=.04) and gene expression of MUC1 and MUC2 (p<.05) in the jejunum. Dietary supplementation of 100mg/kg beta-glucan provoked the up-regulation of Lactobacillus counts and down-regulation of Escherichia coli counts in the caecum (p=.05). Data suggested that improved growth performance in response to beta-glucan supplementation at 100mg/kg in weaned piglets may be explained by the improved intestinal function.