The authors studied the effect of the bacterial-humic preparation AFG-b containing the spore-forming bacteria Bacillus subtilis and Bacillus amyloliquefaciens in a production experiment in the northern forest-steppe of the Novosibirsk Priob’ye region. The preparation was used on midearly spring wheat, which forms grain at the level of valuable wheat. The drug was used as an antistressant together with herbicides against dicotyledonous and monocotyledonous plants. The tank mixture used was metsulfuron-methyl, fenoxaprop-P-ethyl with the antidote cloquintoset-mexyl and 2-ethyl hexyl ester of 2,4-D with florasulam. The bacterial-humic preparation AFG-b is known to level out herbicide stress in wheat and improve plant health. It improves the condition of leaf cell membranes by preventing the escape of electrolytes from the cells. On the roots of plants one month after applying the antistressant, the authors observed a 1.5-2 times reduction in the development and prevalence of root rot of fusarium-helminthosporiosis etiology. The authors also marked the most pronounced phytosanitary effect of the preparation’s bacteria, which are antagonists of phytopatho- gens for plants’ primary roots and epicotyl. An increase in plant productivity manifests the anti-stress and growth-stimulating effect of AFG-b. During the growing season of 2020, the bio preparation combined with herbicides provided a reliable increase of 40.2% of the grain of spring wheat and improved its quality by enhancing the protein and gluten content. Under 2019 conditions, AFG-b increased grain yield relative to herbicides by about 8% and did not affect grain quality. Application of AFG-b as an anti-stressant is not accompanied by improvement of seed quality of the new crop. It does not improve its phytosanitary status in infestation by phytopathogens Bipolaris sorokiniana, Fusarium sp., Alternaria, Stagonospora nodorum, Penicillium and Aspergillus relative to herbicides alone.