Nutrition education during childhood is an evidence-based effective way to foster healthy eating habits and improve health outcomes. The current study aimed to assess the effect of nutrition education on nutrition knowledge and healthy eating practices in middle childhood. The research design was Quasi Experimental Pre test-Post test Without Control Group. One school was selected on the basis of permission and 60 participants aged 6-10 years were selected conveniently. A structured questionnaire was developed by the researcher. It included demographic, knowledge and practice based questions. After the pretest interventions were administered in 3 sessions. The interventions included lecture, demonstration, several worksheets, pamphlets, home activities and interactive activities developed through review of literature. Same questionnaire was used for posttest. Complete data was available for 46 participants only, an attrition rate of 23%. Descriptive statistics, frequencies and percentages were calculated for all continuous and categorical variables using SPSS vs 20. Mean age of the participants was 7.260 years (+ 0.953). 54% were boys and 46% were girls. The post test results showed a remarkable improvement and majority of responses were correct. The participants gave 100% correct responses regarding balanced diet, milk products, green vegetables, fruits, and healthy eating. Practice about daily consumption of lentils/beans (43.5%), cereals (100%), homemade lunch (93.5%), 2 cups milk (82.6%), fruits (82.6%), and vegetables (100%). It was concluded that nutrition education can be effectively used to improve knowledge and practice of healthy eating among school going children, which in turn would result in improvement of their nutritional status.