Improved Detection Limits Research Articles

Spectroscopic performance evaluation and modeling of a low background HPGe detector using GEANT4

Low background gamma spectrometry employing HPGe detectors is a sensitive technique for measuring low-level radioactivity in environmental applications, material screening, and for rare decay searches. This work presents spectroscopic performance evaluation and modeling of a low background measurement setup developed at IIT Ropar in Punjab, India, to measure trace natural radioactive elements, with a particular interest in studying low-level radioactivity in soil and/or rock samples to generate specific inputs for low background experiments. The performance test and characterization of a low background cryocooled HPGe detector with relative efficiency of ∼33% have been carried out. An effective detector model has been developed using GEANT4 Monte Carlo simulation to determine the response of the detector over an energy range of 80.9–1408 keV and compared with the experimental performance of the detector. The response of the detector obtained using Monte Carlo simulations agrees reasonably well within 93% level of confidence, indicating only 7% deviation in the comparison. The present setup offers improved detection limits of primordial radionuclides (U/Th and K) to measure radioactive contamination in environmental matrices, which has been used elsewhere (Thakur, 2023).

In Vivo Profiling and Quantification of Chlorinated Paraffin Homologues in Living Fish.

Herein, we demonstrate the ability of a dual-purpose periodic mesoporous organosilica (PMO) probe to track the complex chlorinated paraffin (CP) composition in living animals by assembling it as an adsorbent-assisted atmospheric pressure chemical ionization Fourier-transform ion cyclotron resonance mass spectrometry (APCI-FT-ICR-MS) platform and synchronously performing it as the in vivo sampling device. First, synchronous solvent-free ionization and in-source thermal desorption of CP homologues were achieved by the introduction of the PMO adsorbent-assisted APCI module, generating exclusive adduct ions ([M - H]-) of individual CP homologues (CnClm) with enhanced ionization efficiency. Improved detection limits of short- and medium-chain CPs (0.10-24 and 0.48-5.0 pg/μL) were achieved versus those of the chloride-anion attachment APCI-MS methods. Second, the dual-purpose PMO probe was applied to extract the complex CP compositions in living animals, following APCI-FT-ICR-MS analysis. A modified pattern-deconvolution algorithm coupled with the sampling-rate calibration method was used for the quantification of CPs in living fish. In vivo quantification of a tilapia exposed to technical CPs for 7 days was successfully achieved, with ∑SCCPs and ∑MCCPs of the sampled fish calculated to be 1108 ± 289 and 831 ± 266 μg/kg, respectively. Meanwhile, 58 potential CP metabolites were identified in living fish for the first time during in vivo sampling of CPs, a capacity that could provide an important tool for future study regarding its expected risks to humans and its environmental fate.

Differential Sensing with Replicated Plasmonic Gratings Interrogated in the Optical Switch Configuration.

A new plasmonic configuration is proposed for application in a sensor and demonstrated for the detection of variations in the bulk refractive index of solutions. The configuration consists of monitoring two diffracted orders resulting from the interaction of a TM-polarized optical beam incident on a grating coupler, operating based on an effect termed the "optical switch". The two monitored diffracted orders enable differential measurements which cancel the drift and perturbations common to both, leading to an improved detection limit, as demonstrated experimentally. The measured switch pattern associated with the grating coupler is in good agreement with theory. Bulk sensing is demonstrated under intensity interrogation via the sequential injection of solutions comprised of glycerol in water into a fluidic cell. A limit of detection of about 10-6 RIU was achieved. The optical switch configuration is easy to implement and is cost-effective, yielding a highly promising approach for the sensing and the real-time detection of biological species.

Pyridoxal derived red-emitting aggregates for the ratiometric sensing of pH and copper(II)

The research on aggregation-induced emission (AIE) continue to gain a bourgeoning interest for their extensive applications in different field including sensing and biosensing. In this study, the red-emitting rod-type aggregates L (λex = 375 nm, λem = 597 nm) was prepared facilely in two steps, i.e., the vitamin B6 cofactor pyridoxal was first condensed with the p-phenylenediamine followed by the DMSO solution of the orange precipitate formed was allowed to self-aggregate by adding poor solvent. Upon self-aggregation of L, the restriction of intramolecular motion and the ESIPT phenomenon play the key role in emitting red fluorescence at 597 nm. The pH dependent emission study of the aggregates L revealed three distinct pH windows: red-emitting between pH 5.4 to 9.1, green emitting above pH 9.9 and non-fluorescent in acidic pH. The aggregates L in HEPES buffer (5% DMSO, 10 mM, pH = 7.4) showed a selective turn-off response towards Cu2+ with a good linearity between 4.8 and 14.7 μM and a detection limit of 2.7 μM. The quenching was occurred due to the non-fluorescent complex formed between L and Cu2+ in the ground state. Addition of strong chelating agent EDTA reversed the quenched fluorescence of L. To improve the practical utility, the turn-off sensor L was converted into a ratiometric sensor for Cu2+ by adding an optimized amount of fluorescein (Fl) dye. The dual emitting L@Fl showed fluorescence quenching at 597 nm whereas concomitant enhancement at 515 nm because of the energy transfer mechanism. The developed ratiometric sensor showed improved detection limit of 0.72 μM. Finally, the practical utility of both L as turn-off sensor and L@Fl as ratiometric sensor was examined by quantifying Cu2+ concentration in different real samples.

Plasmonic Ring Resonator Sensor With High Sensitivity and Enhanced Figure of Merit Using an Ag–Si–Ag Bus Waveguide

A plasmonic miniaturized ring resonator based on Ag-analyte-Ag ring cavity coupled with an Ag-Si-Ag input bus waveguide has been numerically analyzed for bulk refractive index and surface adlayer sensing applications. The opto-geometric parameters of the sensor structure have been thoroughly optimized to maximize field confinement in the sensing zone, which significantly improves the sensitivity. Using an Ag-Si-Ag input bus waveguide results in the reduction of propagation loss which offers an improved quality factor and figure of merit due to narrowing of full width at half maximum (FWHM) of the transmission spectrum. The average bulk refractive index sensitivity over biologically relevant refractive index range (1.33-1.38 RIU) and gas-sensitivity (1-1.01 RIU) values are 1757.71 nm/RIU with an FWHM of 37 nm, and 1787.14 nm/RIU with an FWHM of 24 nm, respectively. We have also investigated the optimized sensor's performance as an adlayer biosensor and the maximum adlayer sensitivity obtained is 2.72 nm/nm. We have also analyzed the sensor's optical tolerance by incorporating a 10% change in Ag's and Si's real part of dielectric constants. Owing to excellent sensitivity and high figure of merit the proposed sensor may find applications in designing highly sensitive on-chip nano-sensors with improved detection limit.

A novel potentiometric sensor based on ZnO decorated polyaniline/coal nanocomposite for diltiazem determination.

Diltiazem (DTZ) is one of the most effective medications for treating cardiovascular diseases. It has been widely used for the treatment of angina pectoris, hypertension and some types of arrhythmia. The development and application of a modified carbon paste sensor with improved detection limits for the potentiometric determination of diltiazem are the main goals of the current study. Sensitivity, long-term stability, reproducibility and improving the electrochemical performance are among the characteristics that have undergone careful examination. A modified carbon paste sensor based on β-cyclodextrin (β-CD) as ionophore, a lipophilic anionic additive (NaTPB) and a ZnO-decorated polyaniline/coal nanocomposite (ZnO@PANI/C) dissolved in dibutyl phthalate plasticizer, exhibited the best performance and Nernstian slope. The ZnO@PANI/C based sensor succeeded in lowering the detection limit to 5.0 × 10-7 through the linear range 1.0 × 10-6 to 1.0 × 10-2 mol L-1 with fast response time ≤ 10.0 s. The prepared nanomaterial was characterized using X-ray diffraction (XRD), Fourier transform infrared (FT-IR) spectroscopy and scanning electron microscopy (SEM). The surface properties of the proposed sensor were characterized by electrochemical impedance spectroscopy (EIS). The selectivity behavior of the investigated sensor was tested against a drug with similar chemical structure and biologically important blood electrolytes (Na+, K+, Mg2+, and Ca2+). The proposed analytical method was applied for DTZ analysis in pure drug, pharmaceutical products and industrial water samples with excellent recovery data.

Implementation of the surface gradiometer receive coils for the improved detection limit and sensitivity in the single-sided MPI scanner

Objective. Magnetic Particle Imaging (MPI) promises to enhance diagnostic capabilities of the existing clinical imaging modalities. Traditional MPI scanners utilize cylindrical bore geometry that prevents scaling up the MPI to accommodate full human subject. Single-sided geometry, on the other hand, has all the hardware located on one side providing an unrestricted imaging volume. Approach. Our single-sided MPI device utilizes a field-free line topology with a single drive coil and a surface receive coil, which is used to detect the nanoparticles. Unlike closed bore systems, single-sided devices cannot adapt well established solenoid gradiometer receive coil, which result in impinging potential sensitivity gain. Main results. In this work we study multiple receive coil configurations with compensation for the purpose of removing feedthrough, whilst preserving the superparamagnetic iron oxide nanoparticle signal. Moreover, we present a compensated surface receive coil design that provides highest sensitivity in the single-sided geometry and demonstrate a new detection limit in a single-sided scanner of 100 ng of iron. In addition, we demonstrate 1D imaging of a sample without use of receive filter recovering signal at fundamental harmonic. Significance. These advancements in the receive chain are crucial for developing a practical MPI scanner with a single-sided geometry.

Relay-type sensing mode: A strategy to push the limit on nanomechanical sensor sensitivity based on the magneto lever.

Supplementary material (experimental section) is available in the online version of this article at 10.1007/s12274-022-5049-0.

Surface modification of poly(styrene) 96-well plates using aptamers via a dendrimer-templated strategy to enhance ELISA performances

Poly(styrene) (PS) 96-well plates were surface modified to improve the detection performances of an otherwise traditional enzyme-linked immunosorbent assay (ELISA). Poly(amidoamine) generation 7 (G7) dendrimers were covalently immobilized on the surface of PS plates and subsequently conjugated with aptamers specific for a model analyte, i.e., human platelet-derived growth factor BB (PDGF-BB). This surface functionalization was followed by Fourier-transform infrared spectroscopy, water contact angle, atomic force microscopy, and X-ray photoelectron spectroscopy (XPS) to confirm the success of the modifications. Moreover, the assay performances of the G7-aptamer modified PS plates were compared to those of traditional ELISA performed on regular PS 96-well plates. The G7-aptamer assay demonstrated a 2.3-time broader linear detection range and a 13-time improved detection limit than the traditional ELISA. More importantly, the new G7-aptamer modified PS plates also showed excellent analytical specificities, detection recoveries, and precisions when the targets were assayed in a cell culture medium. This combined dendrimer templates and aptamers surface modification approach significantly reduces background noises and increases detection signals, and can be readily incorporated into existing ELISA workflows and many other PS microplate based high throughput and automated bioassays.

Electro-plasmonic-assisted biosensing of proteins and cells at the surface of optical fiber

An electro-plasmonic biosensor is used to attract proteins and cells on the surface of a fiber optic probe by controlled biomolecular migration. Concentrating targets on a high performance plasmon-assisted fiber grating sensor leads to a drastic enhancement of the limit of detection. This architecture relies on a biofunctionalized gold coated tilted fiber Bragg grating (TFBG) that operates as a working electrode to enable electrophoresis in the probed medium. The applied electric field triggers the attraction of proteins over a distance of almost 250 μm from the sensor surface, which is more than two orders of magnitude larger than the intrinsic penetration depth of the plasmon wave. Quantitative determination of target analytes was performed by cyclic voltammetry measurements using the gold coated fiber as an electrode, simultaneously with optical transmission measurements of the underlying fiber grating. In our work, these electro-plasmonic optrodes were used against a clinically-relevant biomarker in breast cancer diagnosis, namely HER2 (Human Epidermal Growth Factor Receptor-2). In vitro assays confirm that their limit of detection lies in the subpicomolar range for proteins, which is beyond reach of similar sensors without voltammetry. The improved detection limit is further facilitated by an improvement of the signal-to-noise ratio of the read-out process. Whole cell capture is finally demonstrated by the same micro-system.

Pressed Lateral Flow Assay Strips for Flow Delay-Induced Signal Enhancement in Lateral Flow Assay Strips.

This paper proposes that the signal intensity of a lateral flow assay (LFA) strip can be increased by pressing the top of the strip, effectively reducing its flow rate. The reduced flow rate allows more time for antigen–antibody interactions to occur, resulting in increased signal intensity and an improved detection limit. To assess the potential of the pressed LFA (pLFA) strip, C-reactive protein (CRP) diluted in phosphate-buffered saline (PBS) and serum is detected, affording signal enhancement and a lowered limit of detection. Additionally, to show that the signal enhancement by pressure-induced flow delay applies to existing LFA products, commercially available COVID-19 antigen test strips are pressed, and signal enhancement is observed. Lastly, we show that the signal intensity of COVID-19 LFA kits can be increased by approximately two-fold at maximum by applying pressure on top of the manufactured product. This study suggests that pressed LFA strips can be used to reduce the chances of determining ambiguous signals as false-negative results and can potentially improve the detection sensitivity.Graphical abstract Supplementary InformationThe online version contains supplementary material available at 10.1007/s13206-022-00085-w.

Label-Free and Bioluminescence-Based Nano-Biosensor for ATP Detection.

A bioluminescence-based assay for ATP can measure cell viability. Higher ATP concentration indicates a higher number of living cells. Thus, it is necessary to design an ATP sensor that is low-cost and easy to use. Gold nanoparticles provide excellent biocompatibility for enzyme immobilization. We investigated the effect of luciferase proximity with citrate-coated gold, silver, and gold-silver core-shell nanoparticles, gold nanorods, and BSA-Au nanoclusters. The effect of metal nanoparticles on the activity of luciferases was recorded by the luminescence assay, which was 3-5 times higher than free enzyme. The results showed that the signal stability in presence of nanoparticles improved and was reliable up to 6 h for analytes measurements. It has been suggested that energy is mutually transferred from luciferase bioluminescence spectra to metal nanoparticle surface plasmons. In addition, we herein report the 27-base DNA aptamer for adenosine-5'-triphosphate (ATP) as a suitable probe for the ATP biosensor based on firefly luciferase activity and AuNPs. Due to ATP application in the firefly luciferase reaction, the increase in luciferase activity and improved detection limits may indicate more stability or accessibility of ATP in the presence of nanoparticles. The bioluminescence intensity increased with the ATP concentration up to 600 µM with a detection limit of 5 µM for ATP.

Analysis of As(III) By Anodic Stripping Voltammetry Using Disc and Screen Printed Electrodes

Determination of arsenic is possible by various methods such as spectrophotometry, atomic absorption (as arsine), atomic emission, and neutron activation analysis are common. High levels of interference and the high costs of these methods are major drawbacks. Anodic stripping voltammetry (ASV) has proved to be a sensitive, precise, and cost-effective electroanalytical technique for determining trace metals. It is routinely used for detecting Pb (II) and Cd (II). The general procedure of this technique involves depositing metal(s) at a reductive potential into Hg electrodes for a certain time and stripping them back into the solution by scanning the potential in the oxidative direction. In this work, the analysis of As (III) by ASV has been investigated using solid and Screen Printed Gold electrodes. Improved detection limits up to 10 ppb could be achieved with relative ease. A highly optimized procedure has been presented for the BASi Epsilon users that automatically provides electrochemical pretreatment in automated fashion along with the built-in differential pulse stripping voltammetry in the next step.

Hierarchical Graphene‐Dye Bilayers for Multimodal Optoelectronic Sensing and Decoupling of Complex Stimuli

AbstractSignals obtained by chemical or calorimetric sensors are highly coupled and complex, making it very challenging to precisely detect and discriminate between simultaneous stimuli. The development of sensors that provide multiple sensory outputs is a good way to tackle such a long‐lasting challenge. Herein, a new design of multimodal sensors capable of generating both colorimetric and electrical sensory outputs is introduced. This is achieved by coupling two functional nanolayers, graphene (electrically active) and dyes (colorimetric) in which each layer can work either autonomously or in conjugation with the other sensing layer. It is shown that the interfacial interaction of graphene with the wide variety of dyes creates unique interfacial sensing sites for the detection of chemicals. This endows each sensor or sensor array, in the format of (opto)electronic nose, with a “fingerprint” of varying bonding possibilities, enlarging the spectrum of gas–sensor interactions. Furthermore, it is shown that the hierarchical nanobilayer structure allows the separation and discrimination of volatile organic compounds (VOCs) based on their diffusion kinetics. Taken together, the bilayer design qualifies as a superior sensor compared to unimodal devices by offering improved detection limits, wider dynamic ranges, and higher sensitivity and selectivity in the binary discrimination of a wide range of stimuli (temperature, relative humidity, and VOCs).

Improved detection limits of J-coupled neurometabolites in the human brain at 7T with a J-refocused sLASER sequence.

In a standard spin echo, the time evolution due to homonuclear couplings is not reversed, leading to echo time (TE)-dependent modulation of the signal amplitude and signal loss in the case of overlapping multiplet resonances. This has an adverse effect on quantification of several important metabolites such as glutamate and glutamine. Here, we propose a J-refocused variant of the sLASER sequence (J-sLASER) to improve quantification of J-coupled metabolites at ultrahigh field (UHF). The use of the sLASER sequence is particularly advantageous at UHF as it minimizes chemical shift displacement error and results in relatively homogenous refocusing. We simulated the MRS signal from brain metabolites over a broad range of TE values with sLASER and J-sLASER, and showed that the signal of J-coupled metabolites was increased with J-sLASER with TE values up to ~80 ms. We further simulated "brain-like" spectra with both sequences at the shortest TE available on our scanner. We showed that, despite the slightly longer TE, the J-sLASER sequence results in significantly lower Cramer-Rao lower bounds (CRLBs) for J-coupled metabolites compared with those obtained with sLASER. Following phantom validation, we acquired spectra from two brain regions in 10 healthy volunteers (age 38 ± 15 years) using both sequences. We showed that using J-sLASER results in a decrease of CRLBs for J-coupled metabolites. In particular, we measured a robust ~38% decrease in the mean CRLB (glutamine) in parietal white matter and posterior cingulate cortex (PCC). We further showed, in 10 additional healthy volunteers (age 34 ± 15 years), that metabolite quantification following two separate acquisitions with J-sLASER in the PCC was repeatable. The improvement in quantification of glutamine may in turn improve the independent quantification of glutamate, the main excitatory neurotransmitter in the brain, and will simultaneously help to track possible modulations of glutamine, which is a key player in the glutamatergic cycle in astrocytes.

Enhanced Figure of Merit via Hybridized Guided‐Mode Resonances in 2D‐Metallic Photonic Crystal Slabs

AbstractMetallic nanostructures are highly attractive for refractive index sensing, as the evanescent field from the associated plasmonic resonances resides in close proximity to the adjacent analyte media. However, this benefit is often reduced due to broad plasmonic lineshapes producing poor quality factors. The rational design provides strategies for narrowing the plasmonic modes by incorporating photonic diffraction, which promotes surface lattice resonances . Due to the stringent parametric dependencies, these resonances in metallic lattices are not always feasible, particularly when a straightforward fabrication route with fewer process steps is desired. Herein, hybridized guided‐mode resonance in a 2D‐metallic photonic crystal slab (2D‐mPhCs) is introduced that ensures high‐quality hybrid modes while maintaining a simple fabrication methodology. In direct comparison to its constituent plasmonic and photonic modes, this concept is discussed for sensing applications. The “figure of merit (FOM)” is frequently regarded as a valid metric for measuring sensing performanceensuring high‐quality modes with an improved detection limit. The experimental results confirm enhanced FOM (three to six times) for the hybrid modes, in contrast to the constituent counterparts. For optoelectronic applications, such as photodetection and photocatalysis, these hybrid structures with high‐quality modes offer a promising platform to harvest light at the metal–semiconductor interfaces.

Development of a sensitive sandwich ELISA with broad species specificity for improved fish allergen detection

A sensitive Enzyme-linked Immunosorbent Assay (ELISA) with improved broad species specificity was developed for the detection of southern hemisphere fish residues in processed foods. The polyclonal antibodies were raised against parvalbumins from 13 fish species representing 7 fish orders selected for their molecular diversity and immunoreactivity profile. The optimized ELISA-2 (based on the rabbit capture antibody (RB#4) - sheep detection antibody (S2#4) pair) displayed an improved detection limit of 0.6 μg/L (3.7 μg of /kg). Our immunoreactivity-directed species selection approach in the strategized antibody production significantly improved the detection of no or weakly immunoreactive fish species previously not detected immunochemically. Of 37 commercially important fish species tested, the ELISA-2 could detect 28 fish species (76%). The optimized sample extraction with a buffer additive achieved good protein recoveries of 87.2 – 117.3% (within the AOAC recommended range). The ELISA-2 was able to detect fish residues in five highly processed food products.

Coupling split-lux cassette with a toggle switch in bacteria for ultrasensitive blood markers detection in feces and urine

Cell-based biosensors have powerful abilities to sense a variety of signal chemical molecules. However, compared with commercialized methods, whole-cell biosensors cannot meet the requirements for the lower sensitivity and faster response. Here, we reprogrammed a gene circuit by coupling split-lux cassette with a toggle switch for detecting heme ultra-sensitively. The resultant biosensor (named YES601) exhibited improved detection limit (0.12 ppm) and satisfied maximum induction ratio (more than 4000 folds) for lysed blood detection. Furthermore, we harnessed YES601 to detect the blood signal in the human urine and feces from the mice with DSS-induced colitis, and the results indicated that YES601 showed more satisfied sensitivity and maximum induction ratio compared with chemical method. This ultrasensitive blood biosensor will be applied to detect trace blood in vitro for early-stage diagnosis of serious diseases, and aiding the rapid development for application in diagnosis in vivo in the future.

Abstract 3992: Modular ddPCR assay design for highly sensitive single nucleotide variant detection

Abstract Introduction: Single-nucleotide variants (SNVs) are key drivers of both oncogenesis and emergent resistance to targeted therapies. Although high-throughput Identification of SNVs can be achieved with Next Generation Sequencing (NGS) technologies, the cost is significant, and proportional to the desired sensitivity of variant detection. Conversely, while only small numbers of SNVs can be queried in an individual quantitative PCR (qPCR) or droplet digital PCR (ddPCR) reaction, a fully optimized qPCR or ddPCR assay can detect SNVs at very low frequencies for a fraction of the cost of sequencing. Herein, we present a generalizable approach to PCR assay design, using a combination of mutation-sensitive induced mismatch primers, wild-type suppressors, and TaqMan probes, that permits detection of SNVs below 0.1%. Method: Known oncogenic hotspot regions were selected for development of mutation-detecting PCR assays. For each hotspot, a mutation-specific-mismatch primer, suppressor, and probe (MSMPSP) cassette was designed consisting of 1) a mutation-bearing forward primer with engineered mismatches, 2) a wild-type, extension-locked blocker oligo, 3) a fluorescent TaqMan probe, and 4) a downstream reverse primer. Designed mutation assays were evaluated using plasmid control sequences, remnant clinical specimens, and healthy donor peripheral blood samples spiked with known quantities of mutation-bearing plasmid or cell lines and processed on the BioRad AutoDG/QX200 droplet digital PCR system. Results: Variant detection below 0.1% was achieved for all tested mutations, including those residing in homopolymeric and GC-rich regions, from as low as 100 ng DNA input, in a single day. At higher variant frequencies accessible to multiple platforms, results with MSMPSP-based ddPCR assays exhibited excellent concordance with NGS and qPCR results and yielded linear signal decline down to 0.01% variant allele frequency. Overall assay sensitivity was proportional to DNA input, and improved detection limits were achieved by increased DNA input and combined well analysis. Conclusion: Utilizing the MSMPSP approach, coupled with ddPCR, variant detection for targeted mutations below 0.1% is achievable in diverse genomic contexts. Furthermore, simple design principles and flexible probe-binding sites permit rapid development of new assays, allowing critical mutations to be tracked as they are discovered. While multiplexing capabilities are reduced compared with NGS, short time-to-results, coupled with low cost-per-sample for MSMPSP ddPCR, permits faster treatment decisions at reduced cost to the healthcare system. Citation Format: Sarah Heine, Ashley Beams, Nathan Riccitelli, Shabnam Tangri. Modular ddPCR assay design for highly sensitive single nucleotide variant detection [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2022; 2022 Apr 8-13. Philadelphia (PA): AACR; Cancer Res 2022;82(12_Suppl):Abstract nr 3992.

Specific Ionic Recognition Using Fig's Xylem/Phloem Vessel as a Novel and Applicable Device: Lab-on-a Xylem/Phloem.

A novel and specific detection system using voltage-stimulating ion transport through fig xylem/phloem vessels as a new lab-on-a xylem/phloem substrate was introduced. The voltage drove the ion flux through the vessels by a sinusoidal waveform with very low frequency (2.70 ± 0.05 kHz, n = 10) and voltage amplitude between 0.0 and 1.0 kV (vs total applied potential) with positive and negative polarities depending on cation and anion separation, respectively. The recorded potential induced by the applied potential was considered as a fingerprint electrical potential stimulator during reliable recognition of different ionic species. The system possessed some different characteristics such as (i) prominent figures of merit with linear ranges between 5.0 and 1200.0 (±0.7, n = 10) ng mL–1 (correlation coefficient, R2, >0.99) for each ionic species and (ii) improved detection limits via tracing electrical current and conductance gradient (as the sensitive detection systems), while testing 50.0 ng mL–1 of different salts as cationic and anionic species. The reliability of the system was evidenced via focusing on at least 60 independent cationic and anionic species during introducing a 70-membered distinct array-based bio-substrate device. This process not only showed great method applicability for specific determination with acceptable figures of merit but also resulted in introducing a software database for direct detection and recognition of various ionic analyses. The introduced detection/separation device competed with other spectroscopic/electrochemical systems due to the specific and simultaneous recognition of great ranges of ionic species in different real samples at ultratrace levels.