Improve Crop Productivity Research Articles

Overview of transcriptome changes and phenomic profile of sanitized artichoke vis-à-vis non-sanitized plants.

Plant tissue in vitro culture is increasingly used in agriculture to improve crop production, nutritional quality, and commercial value. In plant virology, the technique is used as sanitation protocol to produce virus-free plants. Sanitized (S) artichokes show increased vigour compared to their non-sanitized (NS) counterparts, because viral infections lead to a decline of growth and development. To investigate mechanisms that control the complex traits related to morphology, growth, and yield in S artichokes compared to NS plants, RNAseq analysis and phenotyping by imaging were used. The role of peroxidases (POD) was also investigated to understand their involvement in sanitized plant development. Results showed that virus infection affected regulation of cell cycle, gene expression and signal transduction modulating cellular response to stimulus/stress. Moreover, primary metabolism and photosynthesis were also influenced, contributing to explain the main morphological differences observed between S and NS artichokes. Sanitized artichokes are also characterized by higher POD activity, probably associated with increased plant growth, rather than strengthening of cell walls. Overall, results show that the differences in development of S artichokes may be derived from the in vitro culture stressor, as well as through pathogen elimination, which, in turn, improve qualitative and quantitative artichoke production.

Potential Improvements in Crop Production in Egypt and Implications for Future Water and Land Demand

Similar to numerous water- and data-scarce regions, Egypt confronts a critical challenge in sustaining food production for its rapidly growing population. Consequently, the country’s water and land resources are under considerable stress and require careful management. About half of Egypt’s both annually harvested areas and renewable freshwater are allocated for cultivating rice, maize, wheat, and berseem clover. However, the extent to which crop production might be improved and how this would impact future water and land requirements remains poorly understood. We analyzed potential improvements in the production of these crops and quantified their future water and land requirements under different scenarios. Potential improvements were detected through percentile analysis in three remote sensing-derived performance indicators for each crop in the Nile Delta’s Zankalon region: (i) crop yield, (ii) crop water productivity, and (iii) transpiration fraction (transpiration to actual evapotranspiration, T/AET). We applied detected improvementsto construct plausible scenarios for Egypt’s water and land requirements to sustain domestic crop production until 2050. Our findings indicate limited potential to improve T/AET (< 4%). However, improvements of up to 27% for crop yields and up to 14% for water productivity are possible. To meet the production targets by 2050, national production must increase by 128, 78, 69, and 71% above the 2016–2020’s average for rice, maize, wheat, and berseem, respectively. Depending on the improvement levels in the developed scenarios, a total harvested land area between 5.3 and 6.4 million ha will be required by 2050, with 18% allocated to rice, 28% to maize, 36% to wheat, and 18% to berseem. Associated freshwater requirements will amount to 59–68 billion cubic meters, divided into 23% for rice, 34% for maize, 28% for wheat, and 15% for berseem. Interventions increasing yields and water productivity will benefit more the summer (rice and maize) than the winter crops (wheat and berseem). We discuss likely interventions for meeting these requirements and for sustaining the supply of these crops in Egypt.

Species interactions and bacterial inoculation enhance plant growth and shape rhizosphere bacterial community structure in faba bean – wheat intercropping under water and P limitations

Grain legumes / cereals intercropping systems with microbial inoculants, hold promise for improving crop productivity under stressful conditions. However, the tripartite interaction involving intercropping system, associated microbiota and stress combining water deficit and low phosphorus (P) availability remains understudied. This study evaluated the impact of three bacterial consortia (C4, C6, and Cref containing Rhizobium and PSB strains) including single Rhizobium (Rhizobium laguerreae) on the agro-physiological performance of wheat (Triticum durum) and faba bean (Vicia faba) grown as intercrops or sole-crops under P and water deficient conditions. Inoculation, especially with C6, significantly improved shoot and root biomasses of both wheat (up to 66 and 81 %) and faba bean (up to 54 and 266 %) intercrops compared to single Rhizobium inoculation and control treatments. Intercropping generally outperformed sole-cropping in above-ground physiology, root morphological traits, shoot and root P content, with a notable effect in response to C6 exhibiting low microbial biomass P. Changes in bacterial community structure were primarily driven by cropping pattern and water regime rather than bacterial inoculation. Intercropping maintained bacterial diversity but shifted community structure, favoring Proteobacteria. Overall, inoculating intercropped wheat and faba bean with Rhizobium-containing consortia induced beneficial below-ground interspecies interactions under water and P-limiting conditions.

Proximal sensor characterization of Haitian agricultural soils: A case study in the Central Plateau

Optimized agronomic production is vital to life and livelihood in Haiti. As such, rapid, low-cost soil characterization may inform specific decision making for improved crop production. Ahead of a larger soil characterization campaign (120,000 points) in Eastern Haiti, this study evaluated the use of two proximal sensors (portable X-ray fluorescence – pXRF; NixPro color sensor) at farms of Association Zanmi Agrikol (AZA) for soil characterization and evaluation of spatial variability of soil properties. Analysis of 166 surface soil samples (0–5 cm) collected in three different areas revealed that similar inter-elemental associations reported in other countries remain applicable in Haiti; weak, yet significant relationships between Al, K, and Fe contents and color parameters (L*, A*, B*) were observed. Considerable variability in soil morphology and chemical properties exist even across short distances, underscoring the need for high density sampling schemes. Soil pH (observed up to 8.4) likely imposes restrictions on the bioavailability of certain micronutrients; soil salinity was low (not agronomically limiting) across the study areas, and reflective of excessive drainage. Soil profile characterization identified one Eutrudept and one Quartzipsamment; both used for agronomic production. The proliferation of skeletal coarse fragments (e.g., cobbles, stones) and sandy matrix with depth emphasize the need for periodic supplemental irrigation and fertilization via either soil amendments (e.g., compost) or commercial fertilizers as water holding capacity and nutrient storage (e.g., clays, organic matter) are likely to be limited. Use of the NixPro color sensor showed differences in topsoil color likely reflective of differential soil organic carbon levels. The proximal sensors applied herein represent their first such use for agronomic management in Haiti and have shown considerable promise for additional study and application.

Vermicompost Supply Enhances Fragrant-Rice Yield by Improving Soil Fertility and Eukaryotic Microbial Community Composition under Environmental Stress Conditions.

Heavy-metal contamination in agricultural soil, particularly of cadmium (Cd), poses serious threats to soil biodiversity, rice production, and food safety. Soil microbes improve soil fertility by regulating soil organic matter production, plant nutrient accumulation, and pollutant transformation. Addressing the impact of Cd toxicity on soil fungal community composition, soil health, and rice yield is urgently required for sustainable rice production. Vermicompost (VC) is an organic fertilizer that alleviates the toxic effects of Cd on soil microbial biodiversity and functionality and improves crop productivity sustainably. In the present study, we examined the effects of different doses of VC (i.e., 0, 3, and 6 tons ha-1) and levels of Cd stress (i.e., 0 and 25 mg Cd kg-1) on soil biochemical attributes, soil fungal community composition, and fragrant-rice grain yield. The results showed that the Cd toxicity significantly reduced soil fertility, eukaryotic microbial community composition and rice grain yield. However, the VC addition alleviated the Cd toxicity and significantly improved the soil fungal community; additionally, it enhanced the relative abundance of Ascomycota, Chlorophyta, Ciliophora, Basidiomycota, and Glomeromycta in Cd-contaminated soils. Moreover, the VC addition enhanced the soil's chemical attributes, including soil pH, soil organic carbon (SOC), available nitrogen (AN), total nitrogen (TN), and microbial biomass C and N, compared to non-VC treated soil under Cd toxicity conditions. Similarly, the VC application significantly increased rice grain yield and decreased the Cd uptake in rice. One possible explanation for the reduced Cd uptake in plants is that VC amendments influence the soil's biological properties, which ultimately reduces soil Cd bioavailability and subsequently influences the Cd uptake and accumulation in rice plants. RDA analysis determined that the leading fungal species were highly related to soil environmental attributes and microbial biomass C and N production. However, the relative abundance levels of Ascomycota, Basidiomycota, and Glomeromycta were strongly associated with soil environmental variables. Thus, the outcomes of this study reveal that the use of VC in Cd-contaminated soils could be useful for sustainable rice production and safe utilization of Cd-polluted soil.

Cu-nanoparticles enhance the sustainable growth and yield of drought-subjected wheat through physiological progress

Drought stress (DS) is a significant abiotic stress that limits agricultural productivity worldwide. In semi-arid climates, one potential solution to alleviate the deleterious effects of drought is the use of soil amendments such as nanoparticles. The current research was conducted out to probe the sway of drought at critical growth stages (CGS) of wheat crop (D0: Control, D1: Drought at tillering stage, and D2: Drought at anthesis stage) and the application of Cu-nanoparticles (T0: 0 mg L−1, T1: 300 mg L−1, T2: 700 mg L−1, and T3: 950 mg L−1) in order to improve drought resilience. Results of the study revealed that DS considerably decreased the wheat growth and yield during CGS. However, Cu-nanoparticles application alleviated the detrimental backlash of DS and led to improvements in various aspects of wheat growth and yield, including plant height, spike length, 1000 grain weight, stomatal conductance, leaf chlorophyll content, water use efficiency, leaf turgor potential, relative water content, and ultimately the grain yield. The use of principal component analysis allowed us to integrate and interpret the diverse findings of our study, elucidating the impact of Cu-nanoparticle treatment on wheat growth and yield under drought. Overall, the study concluded that DS during the anthesis stage had the most significant negative impact on crop yield. However, applying Cu-nanoparticles at the rate of 300 mg L−1 proved to be an effective strategy for improving crop productivity by reducing the harmful effects of drought.

Application of Zinc Oxide Nanoparticles to Mitigate Cadmium Toxicity: Mechanisms and Future Prospects.

Cadmium (Cd), as the most prevalent heavy metal contaminant poses serious risks to plants, humans, and the environment. The ubiquity of this toxic metal is continuously increasing due to the rapid discharge of industrial and mining effluents and the excessive use of chemical fertilizers. Nanoparticles (NPs) have emerged as a novel strategy to alleviate Cd toxicity. Zinc oxide nanoparticles (ZnO-NPs) have become the most important NPs used to mitigate the toxicity of abiotic stresses and improve crop productivity. The plants quickly absorb Cd, which subsequently disrupts plant physiological and biochemical processes and increases the production of reactive oxygen species (ROS), which causes the oxidation of cellular structures and significant growth losses. Besides this, Cd toxicity also disrupts leaf osmotic pressure, nutrient uptake, membrane stability, chlorophyll synthesis, and enzyme activities, leading to a serious reduction in growth and biomass productivity. Though plants possess an excellent defense mechanism to counteract Cd toxicity, this is not enough to counter higher concentrations of Cd toxicity. Applying Zn-NPs has proven to have significant potential in mitigating the toxic effects of Cd. ZnO-NPs improve chlorophyll synthesis, photosynthetic efficiency, membrane stability, nutrient uptake, and gene expression, which can help to counter toxic effects of Cd stress. Additionally, ZnO-NPs also help to reduce Cd absorption and accumulation in plants, and the complex relationship between ZnO-NPs, osmolytes, hormones, and secondary metabolites plays an important role in Cd tolerance. Thus, this review concentrates on exploring the diverse mechanisms by which ZnO nanoparticles can alleviate Cd toxicity in plants. In the end, this review has identified various research gaps that need addressing to ensure the promising future of ZnO-NPs in mitigating Cd toxicity. The findings of this review contribute to gaining a deeper understanding of the role of ZnO-NPs in combating Cd toxicity to promote safer and sustainable crop production by remediating Cd-polluted soils. This also allows for the development of eco-friendly approaches to remediate Cd-polluted soils to improve soil fertility and environmental quality.

Green Microbe Profile: Rhizophagus intraradices—A Review of Benevolent Fungi Promoting Plant Health and Sustainability

Arbuscular mycorrhizal fungi (AMF) such as Rhizophagus intraradices (formerly known as Glomus intraradices) are of great importance to maintaining the soil ecosystem while supporting sustainable agriculture and practices. This review explores the taxonomy of Rhizophagus intraradices, their attributes, mycorrhizal symbiosis, plant growth improvement, nutrient recycling in the soil, soil health and environmental rehabilitation, and challenges that impede the effective use of AMF in agriculture. AMF impacts soil structure by releasing organic compounds like glomalin, improving total organic carbon and water-holding capacity, and reducing water scarcity. AMF, in sustainable agriculture, not only improves crop productivity through nutrient uptake but also enhances soil fertility and plants’ resistance to so-called stress from abiotic factors as well. The integration of AMF with other beneficial microorganisms in organic farming will be powerful both to ensure long-term soil output and to protect food from bacteria. Nevertheless, chemical inputs and spatial biases of the researchers remain matters to be solved in connection with the broad feasibility of AMF use.

WHIRLY proteins, multi-layer regulators linking the nucleus and organelles in developmental and stress-induced senescence of plants.

Plant senescence is an integrated programme of plant development that aims to remobilize nutrients and energy from senescing tissues to developing organs under developmental and stress-induced conditions. Upstream in the regulatory network, a small family of single-stranded DNA/RNA-binding proteins known as WHIRLYs occupy a central node, acting at multiple regulatory levels and via trans-localization between the nucleus and organelles. In this review, we summarize the current progress on the role of WHIRLY members in plant development and stress-induced senescence. WHIRLY proteins can be traced back in evolution to green algae. WHIRLY proteins trade off the balance of plant developmental senescence and stress-induced senescence through maintaining organelle genome stability via R-loop homeostasis, repressing the transcription at a configuration condition, and recruiting RNA to impact organelle RNA editing and splicing, as evidenced in several species. WHIRLY proteins also act as retrograde signal transducers between organelles and the nucleus through protein modification and stromule or vesicle trafficking. In addition, WHIRLY proteins interact with hormones, reactive oxygen species and environmental signals to orchestrate cell fate in an age-dependent manner. Finally, prospects for further research and promotion to improve crop production under environmental constraints are highlighted.

Exploring tolerance mechanisms and root morphological development of New Zealand spinach and quinoa across salinity levels

Soil salinity, root sodium concentration, and transpiration rate are important environmental factors impacting plant growth and productivity. This study investigates the salt-coping strategies of two plant species under varying salinity levels: New Zealand spinach species grown at three salinity levels (i.e., 0, 100, and 200 mM NaCl) and three varieties of quinoa, i.e., “Vikinga”, “Dave 407” and “Red Head”, grown with or without salt (i.e., 0 and 100 mM NaCl). The plants were grown on glass fiber sheets (rhizoslides) under controlled laboratory conditions. The relationship between transpiration rate, salinity, and root architecture was analyzed for each species. The root apparatus of New Zealand spinach was significantly more developed in plants grown at 100 mM NaCl compared to both 200 mM NaCl and control. The quinoa varieties responded differently to the increasing salt concentrations, with salt-treated “Red Head” plants significantly reducing their total root length and salt-treated “Vikinga” significantly reducing its lateral roots compared to the controls. Salt concentration near New Zealand spinach roots was lower compared to areas without roots, suggesting active salt uptake. In contrast, quinoa rhizoslides exhibited higher salt concentration in proximity to the roots, indicative of salt exclusion. The study provides insights into the adaptation and tolerance mechanisms of these two species to salinity, elucidating the mechanisms by which plants regulate their sodium uptake and root growth. The obtained results suggest that not only the selection of appropriate plant species (halophytes), but also varieties, plays a crucial role in improving crop productivity in salt-affected areas. Elucidating the salt-coping mechanisms of halophytes in relation to soil and climatic conditions is important to better predict their behavior in various saline environments.

The Use of Indigenous Knowledge Systems Practices to Enhance Food Security in Vhembe District, South Africa

This paper seeks to examine how subsistence farmers employ indigenous knowledge methods to enhance food security within rural communities in the Vhembe district. It analyses indigenous knowledge practices used in managing climate change impacts on crop production and determines indigenous knowledge processes used to preserve food to fill the gap during food shortages. The paper also considers the indigenous weather prediction approaches used in the area. A participatory rural appraisal is complemented by a qualitative and quantitative approach. This study used semi-structured interviews, questionnaires and focus group discussions for data collection. A survey of 200 randomly selected indigenous farmers was sampled. The finding revealed that local farmers depend on the use of indigenous knowledge practices to improve household food security. The majority of farmers apply manure in their farms using livestock dumps. Also, it shows that 87.8% of households used sun-drying processes for food preservation and used local mortars to pound and grind foodstuffs such as peanuts and maize. Practices such as crop rotations, mixed cropping and intercropping were employed to improve soil fertility and climate change and reduce insect pest outbursts on crops, hence, improving crop production. Indigenous knowledge of rainfall prediction is helpful in preparation for the planting season. The study recommended that agriculture policies must acknowledge indigenous knowledge practices in development programmes and specific policy interventions to promote the indigenous knowledge systems must focus on enhancing socio-economic factors assisting farmers in improving post-harvest storage facilities. The findings could facilitate interaction between indigenous knowledge stakeholders and the Limpopo provincial agriculture training institution. The fulfilment of the United Nations Sustainable Development Goal 2 on zero hunger and food security could benefit from the indigenous knowledge systems.

Assessment of Integrated Poultry Manure and Synthetic Fertilizer Effects on Maize (Zea mays) Growth and Soil Properties: A Study from Bayero University, Kano

The study investigated the effects of organic and inorganic nutrients on crop productivity and soil health, with a focus on maize growth and soil properties. It was conducted at Bayero University, Kano and comprised six treatments laid out in a Completely Randomized Design (CRD) with three replications. The treatments included poultry manure, synthetic fertilizers, and their combination. The research aimed to assess the impact of these treatments on maize growth and their residual effects on soil physical and chemical properties. Various plant growth parameters and soil properties were evaluated, and the data obtained were analyzed using Analysis of Variance (ANOVA) and Student-Newman-Keuls test (SNK). The results indicated that all treatments significantly influenced morphological parameters, including plant height, number of leaves, stem girth, and leaf area, compared to the control. Maize growth was notably higher with the application of integrated poultry and inorganic fertilizer or solely inorganic fertilizers. Additionally, soil organic carbon, total nitrogen, phosphorus, and potassium contents increased when poultry manure was applied alone or in combination with inorganic fertilizer. The application of poultry manure significantly raised soil pH, organic carbon content, total nitrogen, available phosphorus, exchangeable potassium, calcium, magnesium, and effective cation exchange capacity (ECEC) of the soil. Furthermore, the exchangeable acidity of the soil was reduced by the application of poultry manure alone. Soil texture was improved, soil bulk density decreased, and porosity increased due to the application of poultry manure. The study concluded that the combined application of poultry manure and inorganic fertilizer enhanced the growth of maize and could serve as a good soil management practice for tropical soils. It also suggested that integrating inorganic fertilizers with organic manures at optimum rates could improve crop productivity on a sustainable basis. The findings of this study may contribute to the development of sustainable nutrient management programs aimed at enhancing crop productivity with high efficiency and minimal nutrient loss in the future.

Liquid Nano Urea: Step Forward to Smart Agriculture- A Review

Chemical fertilizer is necessary for the growth and development of crops. However, the efficiency of conventional chemical fertilizers is very low. Nowadays, fertilizer use efficiency (FUE) is the focus of agriculture cultivation practices to meet economic and environmental challenges. Around half of the total agricultural production relies on urea fertilizer, and the reduction in soil fertility has led to 3.56%. Liquid Nano Urea (LNU) can play a vital role in ensuring food security in Bangladesh. It can replace 50 % of conventional urea and can save subsidies to the govt. In addition, it is environmentally safe and reduces production costs, ensuring an increase in the total yield of 8-20%. Ensuring 90% work efficiency, the utilization of cutting-edge technologies and modern innovation in agricultural practices to enhance sustainability and efficiency in farming. Only two sprays of LNU can change the whole scenario of food safety. Recommended dose of optimize the use of nano urea for improved crop productivity and sustainability. The proper application of nano urea holds immense potential for enhancing crop production efficiency and sustainability.

Plant pathology identification using local-global feature level based on transformer

Deep learning plays a crucial role in addressing the challenge of plant disease identification in the field of agriculture. Detecting diseases in plants requires extensive effort, along with a comprehensive understanding of various plant diseases and increased processing time. Balancing both speed and accuracy in predicting leaf diseases in plants can significantly improve crop production and reduce environmental damage. In this paper, we examined deseases on popular plants in agriculture. We proposed a novel model to predict crop pathology on a feature space of global-local based on transformer aggregation. Paticular, we use refined feature of different layer to correlate semantics from high-level feature and low-level feature. Besides, to capture the extended temporal scale across the entire image, we employ a transformer to discern long-range dependencies among frames. Subsequently, the enhanced features incorporating these dependencies are inputted into a classifier for preliminary crop pathology prediction. The plant village dataset and VietNam strawberry disease (VNStr) dataset were utilized for training and disease classification in the experiments. Extensive experiments show that the proposed method outperforms by 99.18% and 94.05% accuracy in plant village and VNStr, respectivly. The model after being judged was applied on Android devices and therefore is easy to use.

ZIF-8@Hydroxyapatite Composite as a High Potential Material for Prolonged Delivery of Agrochemicals.

Although agrochemical practices can enhance agricultural productivity, their intensive application has resulted in the deterioration of ecosystems. Therefore, it is necessary to develop more efficient and less toxic methods against pests and infections while improving crop productivity. Moving toward sustainable development, in this work, we originally described the preparation of a composite (ZIF-8@HA) consisting of the coating of zeolitic-like metal-organic framework (MOF) ZIF-8 (based on Zn, an essential micronutrient in plants with antibacterial, antifungal, and antifouling properties) with hydroxyapatite (HA) nanoparticles (i.e., nanofertilizer). The interaction between the HA and ZIF-8 has been characterized through a combination of techniques, such as microscopic techniques, where the presence of a HA coating is demonstrated; or by analysis of the surface charge with a dramatic change in the Z-potential (from +18.7 ± 0.8 to -27.6 ± 0.7 mV for ZIF-8 and ZIF-8@HA, respectively). Interestingly, the interaction of HA with ZIF-8 delays the MOF degradation (from 4 h for pristine ZIF-8 to 168 h for HA-coated material), providing a slower and gradual release of zinc. After a comprehensive characterization, the potential combined fertilizer and bactericidal effect of ZIF-8@HA was investigated in wheat (Triticum aestivum) seeds and Pseudomonas syringae (Ps). ZIF-8@HA (7.3 ppm) demonstrated a great fertilizer effect, increasing shoot (9.4 %) and root length (27.1 %) of wheat seeds after 11 days at 25 °C under dark conditions, improving the results obtained with HA, ZIF-8, or ZnSO4 or even physically mixed constituents (HA + ZIF-8). It was also effective in the growth inhibition (>80 % of growth inhibition) of Ps, a vegetal pathogen causing considerable crop decline. Therefore, this work demonstrates the potential of MOF@HA composites and paves the way as a promising agrochemical with improved fertilizer and antibacterial properties.

Multifaceted Ability of Organic Fertilizers to Improve Crop Productivity and Abiotic Stress Tolerance: Review and Perspectives

The long-term use of chemical fertilizers poses a serious threat to crop productivity and soil quality. Organic fertilizers are used to improve the soil fertility and crop productivity. The application of organic fertilizers improves soil health and plant growth by improving the soil organic matter (SOM), soil structure, aggregate stability, nutrient uptake, water-holding capacity, cation exchange capacity, nutrient use efficiency and microbial activities of soil. The intensity of abiotic stress is continuously increasing, which is a serious threat to crop productivity and global food security. However, organic fertilizers have been reported to improve tolerance against drought, salinity, heat and heavy metal (HM) stresses. The application of organic fertilizer improves the leaf water status, nutrient uptake, nutrient homeostasis, synthesis of chlorophyll, osmolytes, hormones, secondary metabolites, antioxidant activities and gene expression, resulting in improved tolerance against drought, salinity, heat, and heavy metals. In the present review, we have discussed the ability of organic fertilizers to improve soil fertility, crop yield, and the nutrient use efficiency. We have also presented the various mechanisms through which organic fertilizers improve tolerance against drought, salinity, heat, and heavy metals. Therefore, this review will put forth new directions for researchers working on the use of organic materials to improve soil fertility, crop productivity and tolerance against abiotic stresses.

Gender and endogenous knowledge inclusion for agroforestry systems improvement in Benin, West Africa

In West Africa, and Benin particularly, local forest resources can potentially contribute to both increasing and stabilizing soil productivity. However, these resources continue to be neglected with efforts instead concentrated on promoting exotic species. This study aimed to prioritize local agroforestry species on agricultural landscape by investigating the gendered, socio-demographic and agro-pedological factors of local knowledge and use of agroforestry species amongst small-holder farmers in Benin. An agroforestry inventory combined with an ethno-agroforestry survey was conducted on 364 farms with 364 farmers. A cluster analysis based on farmers' socio-demographic and agroecological factors was used to cluster farmers into two homogeneous agroforestry systems. Median score, species diversity and ecological networks were established for these two systems. Results illustrate that gendered difference exist between the priority that farmers give to multi-purpose species and this prioritization depends on priority ecosystem services for farmers and gender. Therefore, it would be useful first to consider gender and specific needs of each category of farmer to optimize the choice of agroforestry species to be promoted in such systems. The successful introduction of identified species through extension programs requires more advanced research related to the real contribution of these species to farmland fertilization, as well as the nutrient transmission pathways to associated crops in an agroforestry system to address simultaneously specific ecological, economic and socio-cultural sustainability criteria, as well as improved crop production.

The Role of Plant Ubiquitin-like Modifiers in the Formation of Salt Stress Tolerance.

The climate-driven challenges facing Earth necessitate a comprehensive understanding of the mechanisms facilitating plant resilience to environmental stressors. This review delves into the crucial role of ubiquitin-like modifiers, particularly focusing on ATG8-mediated autophagy, in bolstering plant tolerance to salt stress. Synthesising recent research, we unveil the multifaceted contributions of ATG8 to plant adaptation mechanisms amidst salt stress conditions, including stomatal regulation, photosynthetic efficiency, osmotic adjustment, and antioxidant defence. Furthermore, we elucidate the interconnectedness of autophagy with key phytohormone signalling pathways, advocating for further exploration into their molecular mechanisms. Our findings underscore the significance of understanding molecular mechanisms underlying ubiquitin-based protein degradation systems and autophagy in salt stress tolerance, offering valuable insights for designing innovative strategies to improve crop productivity and ensure global food security amidst increasing soil salinisation. By harnessing the potential of autophagy and other molecular mechanisms, we can foster sustainable agricultural practices and develop stress-tolerant crops resilient to salt stress.

Augmentation of physiology and productivity, and reduction of lead accumulation in lettuce grown in lead contaminated soil by rhizobacteria-assisted rhizoengineeing

Microbial-assisted rhizoengineering is a promising biotechnology for improving crop productivity. In this study, lettuce roots were bacterized with two lead (Pb) tolerant rhizobacteria including Pseudomonas azotoformans ESR4 and P. poae ESR6, and a consortium consisted of ESR4 and ESR6 to increase productivity, physiology and antioxidants, and reduce Pb accumulation grown in Pb-contaminated soil i.e., 80 (Pb in native soil), 400 and 800 mg kg−1 Pb. In vitro studies showed that these strains and the consortium produced biofilms, synthesized indole-3-acetic acid and NH3, and solubilized phosphate challenging to 0, 100, 200 and 400 mg L−1 of Pb. In static conditions and 400 mg L−1 Pb, ESR4, ESR6 and the consortium adsorbed 317.0, 339.5 and 357.4 mg L−1 Pb, respectively, while 384.7, 380.7 and 373.2 mg L−1 Pb, respectively, in shaking conditions. Fourier transform infrared spectroscopy results revealed that several functional groups [Pb–S, M − O, O–M–O (M = metal ions), S–S, PO, CO, –NH, –NH2, C–C–O, and C–H] were involved in Pb adsorption. ESR4, ESR6 and the consortium-assisted rhizoengineering (i) increased leaf numbers and biomass production, (ii) reduced H2O2 production, malondialdehyde, electrolyte leakages, and transpiration rate, (iii) augmented photosynthetic pigments, photosynthetic rate, water use efficiency, total antioxidant capacity, total flavonoid content, total phenolic content, and minerals like Ca2+ and Mg2+ in comparison to non-rhizoengineering plants grown in Pb-contaminated soil. Principal component analysis revealed that higher pigment production and photosynthetic rate, improved water use efficiency and increased uptake of Ca2+ were interlinked to increased productivity by bacterial rhizoengineering of lettuce grown in different levels of Pb exposures. Surprisingly, Pb accumulation in lettuce roots and shoots was remarkably decreased by rhizoengineering than in non-rhizoengineering. Thus, these bacterial strains and this consortium could be utilized to improve productivity and reduce Pb accumulation in lettuce.

Assessment of the impact of irrigating with treated wastewater on soil and crop trace metal accumulation

ABSTRACT Water scarcity is growing quickly, especially in the arid and semi-arid regions along with the growing population. So, using treated wastewater in agricultural irrigation presents a sustainable approach, enhancing soil quality, increasing organic carbon levels, and improving crop productivity are achieved through this approach, concurrently reducing the need for chemical fertilizers. Yet, escalating industrial effluent discharge raises concerns regarding trace metals in wastewater and associated health risks. Our study in the Tenth of Ramadan City focused on employing secondary treated mixed industrial and municipal wastewater for olives, mandarins, and guavas. Sampling wastewater, soil, and plant parts, we analyzed their properties and trace element concentrations, employing Visual MINTEQ for geochemical speciation. Adhering to the international guidelines, the wastewater exhibited acceptable pH and alkalinity but showed concerning trace element levels for irrigation. Differences in soil composition and trace element concentrations suggest potential contamination in soils irrigated with wastewater, raising concerns about health risks associated with elevated metal levels in plants. Variations in trace element concentrations in guavas, olives, and mandarins’ leaves and fruits were observed, with efficient translocation in olives, while mandarin and guava displayed reduced efficiency. Bioaccumulation Factor trends indicated species-specific metal uptake differences. Waste “Minimization Prioritization Tool categorized” trace element levels as of low concern. Daily Intake of Trace Metals and Health Risk Index highlighted the need for health risk assessment, especially for elevated Ni in mandarins and guavas. Regression findings emphasized soil factors influencing metal uptake in plants, highlighting the need for vigilant soil management to curb trace element contamination in agriculture, ensuring both food safety and environmental health. Therefore, water reuse adds contributes to sustainable development, which is three dimensions; Economic, Social and Environmental. It improves economics to utilize water that would be otherwise wasted, and by making water more productive. It improves the social dimension by addressing water scarcity and satisfying the increasing water demand for sustainable livelihoods. It also improves the environment by increasing wastewater treatment levels and reducing pollution.