Imprinting Sites Research Articles

Synthesis and sorption properties of heparin imprinted zeolite beta/polydopamine composite nanoparticles

Heparin-specific molecularly imprinted polymer (MIP) using a nano-layer of polydopamine was synthesized at the surface of zeolite Beta nanoparticles. The Brunauer–Emmett–Teller (BET) surface area of MIP is about 347.23 m2/g with a mean pore diameter of nearly 8 nm, while the corresponding features for the non-imprinted polymers (NIP) are 255.50 m2/g and 10.17 nm, respectively. The transmission electron microscope (TEM) results revealed that the thickness of the polydopamine shell in NIP is less than that of MIP. The static and selective sorption of heparin along with its sorption kinetics were investigated at physiological pH. The results demonstrated that the sorption isotherm of heparin using NIP follows the Langmuir isotherm model, suggesting the monolayer sorption. However, the Freundlich model presented a better description of heparin sorption by MIP, which indicates the presence of high-affinity binding sites and surface heterogeneity. The pseudo-second-order model demonstrated satisfactorily the kinetic data, indicating the secondary reaction of heparin with imprinting sites through chemical sorption. The selectivity of MIP was assessed using sodium alginate as a similar compound, providing imprinting factor and selectivity coefficient of ca. 10.37 and 9.66, respectively. Finally, the reusability of MIP was studied in three sequential sorption-desorption cycles. Interestingly, after 3 cycles, MIP did not exhibit a significant reduction in binding capacity.

Molecularly imprinted poly (4,4′-methylenedianiline) as electrochemical sensor for determination of 1-benzothiophene

Self-cross linked poly (4.4′-methylenedianiline) as a conductive polymer was prepared via oxidative polymerization of 4.4′-methylenedianiline. Synthesis of poly 4.4′-methylenedianiline in acidic condition, leads to formation of phenazine structural units. Molecularly imprinted poly 4.4′-methylenedianiline, synthesized using 1-benzothiophene as template. The π-π stacking effect between 4.4′-methylenedianiline and 1-benzothiophene, made the compact imprinted polymer and more imprinted sites. Field emission scanning electron microscopy, FT-IR spectroscopy, X-ray diffraction and differential scanning calorimetry were used to characterize the morphology and structure of the poly- (4.4′-methylenedianiline). Detection of 1-benzothiophene was performed with molecularly imprinted poly- (4.4′-methylenedianiline) modified electrode. Cyclic voltammetry was used to character the molecular imprinted polymer and nonimprinted polymer.

Nano layered ion imprinted polymer based electrochemical sensor and sorbent for Mn (II) ions from real samples

In this study, a multiwalled carbon nanotube based ion-imprinted polymer was prepared for the sensing and extraction of manganese ion. The binding sites for adsorption of Mn(II) ion were synthesized by free radical polymerization. For the comparison purpose ion imprinted polymer without using MWCNT was also prepared (MWCNT-NIP). To find out importance of MWCNT, ion imprinted polymer without MWCNT was also synthesized (IIP and NIP). The synthesized polymers were characterized by FT-IR, XRD, TEM, EDAX, and TGA. An electrochemical sensor was made by modifying a platinum electrode with MWCNT-IIP. The electrochemical response of the nanostructures modified platinum electrode were investigated and optimized. The limit of detection of the electrochemical sensor was investigated using DPV, it was found to be 0.0138 µM. The applicability of the modified electrochemical sensor was effectively applied for the sensing and extraction of Mn(II) ion from real samples collected from lake water, fertilizers, and mining effluents. The Mn(II) ion imprinted MWCNT-IIP exhibit Langmuir adsorption isotherm and pseudo-second-order kinetics which proposed homogenous monolayer formation of imprinting sites on the polymer sorbent. The selectivity studies were carried out using imprinted and non-imprinted polymers, the imprinted sorbent shows high selectivity and specificity towards Mn(II) ion than other metal ions.

Molecularly Imprinted Phase-Change Microcapsule System for Bifunctional Applications in Waste Heat Recovery and Targeted Pollutant Removal.

An innovative design of a molecularly imprinted phase-change microcapsule (MIM) system for bifunctional applications in waste heat recovery and targeted pollutant removal was reported in this work. This molecularly imprinted system was successfully constructed by encapsulating n-eicosane with a SiO2 base shell through emulsion-templated interfacial polycondensation and then coating a molecularly imprinted polymeric layer with bisphenol A (BPA) as a template molecule through surface free-radical polymerization. The morphology, microstructure, and chemical structure of the resultant molecularly imprinted phase-change microcapsules (MIMs) were characterized, and their phase-change behavior, thermal energy-storage performance, and selective adsorption capability were investigated intensively. The MIMs developed in this study achieved an outstanding latent heat-storage capability with a high capacity more than 165 J/g and also showed an excellent phase-change reliability with a very small fluctuation in phase-change temperatures and enthalpies after 500 thermal cycles. Moreover, the MIMs also presented a high thermal stability over 200 °C and good shape stability up to 120 °C. Most of all, an effective specific recognition capability and high recognition efficiency were achieved for the MIMs due to the formation of BPA-molecular imprinting sites on their surface. As a result, the MIMs exhibited good adsorption selectivity toward the BPA molecules and satisfactory reusability for targeted removal of BPA with a removal efficiency of 61.7% after 10 cycles of the rebinding-elution procedure. In view of a smart combination of thermal energy-storage and selective adsorption functions, the MIMs developed in this study demonstrate a great potential in applications for waste heat recovery and targeted pollutant removal of industrial and domestic wastewaters.

Preparation and Characterization of Hybrid Molecularly Imprinted Polymer Membranes for the Determination of Citrinin in Rice

A new method for the determination of Citrinin (CIT) in rice samples by hybrid molecularly imprinted polymer (MIP) membrane prior to its quantification by high performance liquid chromatography with fluorescence detection (HPLC-FD) is described for the first time. Conventional extraction methods, such as liquid-liquid extraction (LLE) and solid phase extraction (SPE) produce large volumes of environmentally hazardous waste and the common sorbents used in SPE often suffered from low selectivity. Hybrid MIP membranes offer the advantage of combining the mechanical integrity of the support membrane and the selectivity of the imprinted polymer. These membranes offer large specific surfaces, providing relatively high imprinting sites per unit mass, and fine porous structures, resulting in accessibility of imprinting sites. Thus, MIPs for CIT with 1-naphthol as mimic template were prepared using divinylbenzene as crosslinker and naphthol methacrylate was hybridized into the polyethersulfone scaffold by phase inversion process. The prepared hybrid MIP membrane was characterized using Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). Using the resultant hybrid MIP membranes as sample preparation for HPLC-FD of CIT, detection and quantification limits of 0.5 ng g-1 and 1.7 ng g-1, respectively, were obtained. The intra-day and inter-day precision expressed in %RSD ranged from 1.9-2.9% and 2.6-5.9%, respectively. The recoveries of CIT in rice spiked at 5, 25 and 100 ng g-1 ranged from 89.7-94.2%. Thus, the hybrid MIP membranes can be valuable material for the practical determination of CIT in rice extracts.

Oxidized carbon materials cooperative construct ionic imprinted cellulose nanocrystals films for efficient adsorption of Dy(III)

Selectively extracting high-value rare earth elements from scrap rare earth products is a measure that combines economic and environmental benefits. In this paper, TEMPO-mediated oxidation of cellulose nanocrystals (CNCs) acted as film units, oxidized carbon materials were involved in the cooperative construction of high-performance CNC composite films, and applied them for selective adsorption of Dy(III). The carboxyl groups on multi-walled carbon nanotube and graphene oxide provide additional binding sites with Dy(III), thereby achieving improvement on mechanical properties and adsorption performance. Based on the surface ion-imprinted polymers, stable imprinted structure sites were distributed on the surface of films, which could effectively improve adsorption capacity and selectivity. When pH was 4.0, saturated adsorption capacities of CNC films were in the range of 22.57–34.03 mg g−1. Further, in selective experiments, materials exhibited preferential adsorption for Dy(III) with a partition coefficient of 872.266 mL g−1. Reusability tests revealed film materials have a strong regeneration performance. Overall, the green, highly efficient and non-toxic CNC composite films are expected to provide a novel method for recycling REEs.

Fluorometric microplate-based dimethoate assay using CdSe/ZnS quantum dots coated with a molecularly imprinted polymer.

A microplate-based assay is described for the sensitive and selective fluorometric determination of the pesticide dimethoate. Molecularly imprinted polymer (MIP)-coated CdSe/ZnS quantum dots (QDs) are used as the molecular recognition probe. The MIP-coated QDs were synthesized using one-step reversed-phase microemulsion in the presence of hydrophobic CdSe/ZnS QDs. Copolymerization was performed by using 3-aminopropyltriethoxysilane as the functional monomer, tetraethoxysilane as the cross-linker, and dimethoate as thetemplate. The fluorescence of the coated QDs is quenched by dimethoate when dimethoate becomes rebound on the imprinting sites of the MIP. Under the optimal conditions, fluorescence (best measured at excitation/emisison wavelengths of 380/620nm) drops linearly in the 5.0-150μgL-1dimethoate concentration range, and the limit of detection is 2.1μgL-1 (atS/N= 3). The assay was utilized for dimethoate determination in spiked real samples. Satisfactory recoveries (89.8%-98.0%) with relatively standard deviations of <4.9% are obtained. The method is rapid, cost-effective, sensitive, and selective. The use of microplate allows for the quantitation of a large number of samples simultaneously. Graphical abstract Schematic representation of sensitive and selective fluorometric microplate-based assay for the high-throughput determination of dimethoate (DM) based on recognition of molecularly imprinted polymer (MIP)-coated CdSe/ZnS quantum dots (QDs). DM exerts aquenching effect on the fluorescence of theQDs.

Study of solvent effect on the synthesis of magnetic molecularly imprinted polymers based on ultrasound probe: Application for sulfonamide detection.

In this work, a comparative study of the effect of various solvents on the synthesis of magnetic molecularly imprinted polymers (MMIPs) based on the use of high-power ultrasound probe is reported for the first time. Dimethylsulfoxide (DMSO), dimethylformamide (DMF), ethanol, acetonitrile and acetone were studied as solvents for the synthesis of MMIPs. Several crucial experimental conditions such as the time of synthesis and the applied amplitude were investigated. DMSO, DMF and ethanol were successfully used for ultrasound-assisted synthesis of MMIPs. However, for the polymerization performed using acetonitrile and acetone, no significant conversion to product was observed. Under optimal conditions for each solvent tested, the synthesized MMIPs were characterized using several techniques such as Scanning/Transmission Electron Microscopy (SEM and STEM modes), X-Ray Diffraction, Fourier Transform Infra-Red Spectroscopy, Thermal Gravimetric Analysis and Vibrating Sample Magnetometer system. The study of adsorption time of MMIPs showed that fast adsorption occurred due to the presence of specific imprinted sites on the surface. Moreover, isotherm study showed that the experimental equilibrium data fitted well with Freundlich model. The results of selectivity study indicated that MMIPs could selectively recognize the target molecule. Due to its high adsorption properties and easiness of preparation, MMIP-DMSO was used successfully as adsorbent material in solid-phase extraction coupled to a colorimetric method for sulfamethoxazole (SMX). After optimizing analytical conditions, a calibration plot was performed in the concentration range from 0.2 to 5 µg·mL-1 with limits of detection and quantitation of 0.06 and 0.2 µg·mL-1, respectively. The developed procedure was applied successfully for SMX determination in spiked tap and mineral waters showing satisfactory recoveries. Besides, reusability study demonstrated that MMIP could be reused at least 8 times keeping good binding capacity.

Novel three-Dimensional molecularly imprinted polymer-coated carbon nanotubes (3D-CNTs@MIP) for selective detection of profenofos in food

A new and facile method for selective measurement of profenofos (PFF) using a simple flow-injection system with a molecularly-imprinted-polymer-coated carbon nanotube (3D-CNTs@MIP) amperometric sensor is proposed. The 3D-CNTs@MIP was synthesized by successively coating the surface of carboxylated CNTs with SiO2 and vinyl end groups, then terminating with molecularly imprinted polymer (MIP) shells. MIP was grafted to the CNT cores using methacrylic acid (MAA) monomer, ethylene glycol dimethacrylate (EGDMA) as cross linker, and 2,2′-azobisisobutyronitrile (AIBN) as initiator. We constructed the PFF sensor by coating the surface of a glassy carbon electrode (GCE) with 3D-CNTs@MIP and removed the imprinting template by solvent extraction. Morphological and structural characterization reveal that blending of the MIP on the CNT surface significantly increases the selective surface area, leading to greater numbers of imprinting sites for improved sensitivity and electron transfer. The 3D-CNTs@MIP sensor exhibits a fast response with good recognition when applied to PFF detection by cyclic voltammetry and amperometry. The PFF oxidation current signal appears at +0.7 V vs Ag/AgCl using 0.1 M phosphate buffer (pH 7.0) as the carrier solution. The designed 3D-imprinted sensor provides a linear response over the range 0.01–200 μM (r2 = 0.995) with a low detection limit of 0.002 μM (3σ). The sensor was successfully applied to detection of PFF in vegetable samples.

Sensitive lateral flow immunoassay for the detection of hepatitis B virus surface antigen

Bisphenol A molecular imprinted adsorbent (BMIA) was successfully synthesized by a sol-gel process and showed a good specific binding performance in the water. The further studies showed that the mass transfer process was controlled by in-diffusion, and the synthesis conditions would effect on the amount of imprinting sites. Scatchard model analysis evidenced that the high binding affinity sites and the low binding affinity sites were both on BMIA, and the high binding affinity sites played a key role in the specific binding process. Scatchard model analysis of temperature effect experiments and dosage effect experiments proved that the specific binding sites with high binding affinity and the unexpressed specific binding sites with low binding affinity were the two different states of the imprinting binding sites. The conversion between the two different states depended on the reaction driving force, and the increasing reaction driving force would increase the number of specific binding sites. Especially, the temperature showed a linear positive correlation with the amount of specific binding sites. Finally, a possible model was put forward to explain the two different states conversion mechanism of the imprinting sites.

SYNTHESIS AND CHARACTERIZATION OF A PERIODIC MESOPOROUS ORGANOSILICA MOLECULAR IMPRINTED POLYMER FOR REMOVAL OF DDT

A Periodic Mesoporous Organosilica (PMO) is an organic-inorganic hybrid composition with ordered porous structure on a molecular imprinted polymer (MIP). A triblock copolymer Pluronic F127 poly(ethyle ne glycol)- poly(propylene glycol)-poly(ethylene glycol) as structure directing agent (SDA) and 1,4-bis(triethoxysilyl)benzene (BTEB) as a precursor were used to synthesis the PMO-MIP using a non-covalent approach. The non-covalent approachshowed a limited number of selective imprinted sites due to the lack of strong intermolecular attractions between DDT template and the BTEB precursor. However, treatment of PMO-MIP with chlorotrimethylsilane (CTMS) significantly reduced the SiO-H and Si-OH resulting in a more non-polar material. The synthesized PMO-MIP was able to recover 106%, 85% and 99% of DDT, DDD and DDE respectively which shows that it can be used not only for extraction of DDT and its metabolites from environmental samples but also as a clean-up for samples.

Photo-assisted electrochemical detection of bisphenol A in water samples by renewable {001}-exposed TiO2 single crystals

Bisphenol A (BPA) is a semi-persistent environmental endocrine disrupter and widely present in aqueous environments. Electrochemical detection is an effective method to monitor pollutants like BPA in aqueous environments. However, the electrode fouling from anodic polymeric products is one main barrier of electrochemical sensors for their practical applications. In this work, a renewable electrochemical sensor was rationally designed, constructed and tested for efficient BPA detection. The TiO2 anodic material was surface-engineered by inorganic-framework molecular imprinting sites with tailored morphological shape, exposed facet and crystal structure. This electrode could be activated mainly as an electrochemical catalyst and partially as a photochemical catalyst. The developed TiO2-based sensor exhibited a good detection reliability and cyclic stability for determining BPA in water samples, with an electrochemical signal decrease of less than 5.0% in 10-run cyclic tests. By virtue of the bi-functional properties of the tailored TiO2 anodic material, a unique photo-assisted electrochemical sensor was further developed, in which analyte digestion and analytical signal originated mainly from anodic conversion. Such a synergistic digesting mechanism distinguishes it from the reported electro-assisted photochemical TiO2 sensors. Our work provides a robust sensor for monitoring pollutants in aqueous environments and a new opportunity to develop renewable electrode materials with good reusability.

Effect of gel diffusion on the frontal sorption and desorption of erythromycin by molecularly imprinted polymeric monoliths

ABSTRACTThe frontal dynamics of the sorption and desorption of the antibacterial antibiotic erythromycin on molecularly imprinted monoliths on the base of 2-hydroxyethyl methacrylate and ethylene glycol dimethacrylate and on non-imprinted analog was investigated. In contrast to large molecules, the sorption of the antibiotic was determined not only by convective mass transfer, but also by gel diffusion into weakly cross-linked regions of the polymer sorbents and molecular imprinting of monoliths polymer matrix resulted in an increase in gel diffusion. At desorption from molecularly imprinted polymeric monoliths, an individual front of erythromycin desorption from specific imprint sites was observed.

Molecular structure and spectral characteristics of hyperoside and analysis of its molecular imprinting adsorption properties based on density functional theory

The structure of hyperoside was optimized according to the skeletal types of different galactopyranosides in hyperoside at the DFT/B3LYP/6–31++G(d,p) level, and the frequencies were calculated. The accuracy of the theoretical calculations of the 1H and 13C NMR signals was evaluated by linear correlation. The excited state was calculated via time–dependent density functional theory (TD–DFT). The stable conformation, NMR, UV–vis, natural bond orbital (NBO), molecular electrostatic potential (MEP) and thermodynamic information were obtained. In the most stable conformation of hyperoside, seven intramolecular hydrogen bonds are formed, which affect the imprinting efficiency. The theoretical 1H and 13C NMR results are in good agreement with the experimental results. The B3PW91 function is more suitable than B3LYP for TD–DFT calculations. Combining the UV–vis and NBO analyses, the HOMO→LUMO transition mainly results from the n→π∗ transition of the phenolic hydroxyl groups and the π→π∗ transition of the benzene ring on the B ring in ethanol. The HOMO-1→LUMO+1 transition mainly results from the n→π∗ and π→π∗ transition on the A ring. The MEP and NBO calculations indicate that the imprinted active sites are mainly located on the carbonyl oxygen atom and the hydroxyl hydrogen atoms. As the temperature increases, the molecular heat capacity, entropy, enthalpy, and activity of the imprinting sites increase. The obtained results provide strong theoretical guidance for hyperoside in the synthesis of molecularly imprinted polymers and separation techniques.

Two different states conversion mechanism of the imprinting sites

Bisphenol A molecular imprinted adsorbent (BMIA) was successfully synthesized by a sol-gel process and showed a good specific binding performance in the water. The further studies showed that the mass transfer process was controlled by in-diffusion, and the synthesis conditions would effect on the amount of imprinting sites. Scatchard model analysis evidenced that the high binding affinity sites and the low binding affinity sites were both on BMIA, and the high binding affinity sites played a key role in the specific binding process. Scatchard model analysis of temperature effect experiments and dosage effect experiments proved that the specific binding sites with high binding affinity and the unexpressed specific binding sites with low binding affinity were the two different states of the imprinting binding sites. The conversion between the two different states depended on the reaction driving force, and the increasing reaction driving force would increase the number of specific binding sites. Especially, the temperature showed a linear positive correlation with the amount of specific binding sites. Finally, a possible model was put forward to explain the two different states conversion mechanism of the imprinting sites.

Facile Preparation of a Bacteria Imprinted Artificial Receptor for Highly Selective Bacterial Recognition and Label-Free Impedimetric Detection.

The effective identification and quantification of pathogenic bacteria is essential for addressing serious public health issues. Here, we demonstrate a simple and universal impedimetric sensor for highly selective and sensitive detection of pathogenic bacteria based on the recognition by a bacteria-imprinted polypyrrole (BIP) film. The BIP film was facilely prepared via one-step electro-polymerization followed by in situ removal of the bacterial template. The film structure is novel with noncavity-like imprinted sites situated at the surface of the polypyrrole (PPy) matrix, which are more accessible for the target bacteria and should enhance the mass transfer and the binding kinetics. A limit of quantitation low to 103 CFU/mL was achieved within 1 h for the detection of E. coli O157:H7, which is comparable to the antibody-based assays. Moreover, the sensor displayed remarkable selectivity, especially regarding the specific identification of bacterial serotypes. When employed to analyze E. coli O157:H7 in real drinking water, apple juice, and milk samples, the sensor showed recoveries from 96.0% to 107.9% with relative standard derivations (RSDs) less than 4%. The BIP-based sensing strategy provides a universal approach for specific, selective, and rapid detection of pathogenic bacteria. As compared to conventional biosensors based on biomolecular recognition, this sensor shows clear advantages including easy-of-preparation, robustness, and low cost, which may hold great potential in fields of food/public safety monitoring.

A highly sensitive and selective electrochemical sensor based on polydopamine functionalized graphene and molecularly imprinted polymer for the 2,4-dichlorophenol recognition and detection

In this work, a sensitive and selective electrochemical sensor based on polydopamine-reduced graphene oxide (PDA-rGO) and molecular imprinted polymers (MIP) modified glassy carbon electrode for detection of 2,4-DCP was fabricated. The PDA-rGO was obtained through the auto polymerization of dopamine in graphene oxide solution at an alkaline environment. The MIP film was performed on the surface of the PDA-rGO modified electrode by electropolymerization the monomer of o-phenylenediamine (o-PD) with the template of 2,4-dichlorophenol (2,4-DCP). The bare hydroxyl and benzene rings of PDA-rGO could attract positive charged o-PD and provide π-π stacking effect with o-PD and 2,4-dichlorophenol, which made the compact imprinted film and more imprinted sites. The Electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) were used to character the MIP and NIP modified electrode in the presence of [Fe(CN)6]3-/[Fe(CN)6]4- as a probe for signal transduction. The relative current intensity of ferro/ferricyanide decreased linearly with increasing concentration of 2,4-DCP with a detection limit of 0.8 nM (S/N = 3). The MIP sensor had a much higher affinity towards 2,4-DCP than other analogues. The proposed method was successfully applied for the determination of 2,4-DCP in real water samples.

Cholesterol recognition system by molecular imprinting on self-assembled monolayer

In this study, the molecular imprinting technique and self-assembled monolayer technique were combined to increase the recognition ability of cholesterol. A self-assembled monolayer with 4-mercaptophenol and benzenethiol was formed on a gold plate, and the reaction of cholesteryl chloroformate with the phenol group in the 4-mercaptophenol was followed. This layer was then coated with poly(methyl methacrylate) (PMMA). In order to expose the cholesterol buried under the coating, the partial upper part of the coated PMMA was then removed by stroking the coated plate in acetone solvent. The molecular imprinted site was prepared by hydrolysis of the carbonate bond and extraction of the hydrolyzed cholesterol. This gold plate was used as a working electrode to test the recognition ability for cholesterol. The result showed that the plate obtained good recognition ability for cholesterol compared with cholic acid. The ratio of 4-mercaptophenol to benzenethiol was a very important factor in the ability to recognize cholesterol. Regulating the thickness of the coated PMMA was also one of the important factors to increase the ability to recognize cholesterol.

Chitosan as a substrate for simultaneous surface imprinting of salicylic acid and cadmium

The present work demonstrates functionalized chitosan as an ecofriendly substitute to the conventional costly substrates and monomers for simultaneous surface imprinting of salicylic acid (SA) and cadmium (Cd). Dual surface imprinted acrylamide functionalized chitosan based polymer (AGDMIP), with higher numbers of imprinted sites for SA and Cd was synthesized using acrylamide grafted chitosan, epichlorohydrin as crosslinker, Cd as template and 4 hydroxy benzoic acid (4HBA) as mimic template (supported by computational modeling). FTIR, SEM, XRD, BET surface area and TEM analysis confirmed successful preparation, mesoporous nature and surface imprinting of AGDMIP. The adsorption data could be fitted into Langmuir isotherm model with the maximum adsorption capacity of 45.77 mg g−1 (SA) and 53.42 mg g−1 (Cd). Temkin and Intraparticle diffusion models confirmed the chemical nature and presence of imprint sites within AGDMIP respectively. AGDMIP could be reused for six cycles and exhibited good removal efficiency in real samples.

Specific uptake luteolin by boronate affinity-based single-hole hollow imprinted polymers sealed in dialysis bags

Boronate affinity-based single-hole hollow imprinted polymers (BA-H-MIPs) were fabricated and sealed in dialysis bags for highly specific adsorption of cis-diol containing luteolin (LTL). This preparation strategy is implemented through the direct imprinted polymerization reaction occurring on the surface of carboxyl-capping polystyrene colloids (CPS), and the creating mechanism of single hole is the concomitant microphase separation and symmetrical volume shrinkage of imprinted shells. BA-H-MIPs possess uniform size (200 nm), single holes (50 nm) and hollow structure, and high-affinity sites density (0.27 mg m−2). In static adsorption, BA-H-MIPs show specific high-capacity uptake of target LTL at 318 K (46.96 mg g−1), fast capture kinetics (200 min), remarkable selectivity and regeneration performances. By a pH-responsive purification process, a commercially available 85% LTL could be simply concentrated to 95.88% by BA-H-MIPs, and the purified products have the similar antibacterial performance with standard substance. In addition, the special structure of hollow interior surfaces and single holes provide high site accessibility and abundant imprinted sites, which is the key factor for improving selective binding performance.