Imprinting Effects Research Articles

A Bayesian Multivariate Gametic Model in a Reciprocal Cross with Genomic Information: An Example with Two Iberian Varieties.

INGA FOOD, S.A. initiated a crossbreeding program between two Iberian pig varieties, Retinto (R) and Entrepelado (E), with the goal of producing a hybrid sow (F1). Several studies have been conducted to evaluate its productive performance, and these studies have revealed differences in litter size between the two reciprocal crosses, suggesting the presence of genomic imprinting effects. To further investigate these effects, this study introduces a multivariate gametic model designed to estimate gametic correlations between paternal and maternal effects originating from both genetic backgrounds involved in the reciprocal crosses. The dataset consisted of 1258 records (the total number born-TNB and the number born alive-NBA) from 203 crossbred dams for the Entrepelado (sire) × Retinto (dam) cross and 700 records from 125 crossbred dams for the Retinto (sire) × Entrepelado (dam) cross. All animals were genotyped using the GeneSeek® GPP Porcine 70 K HDchip (Illumina Inc., San Diego, CA, USA). The results indicated that the posterior distribution of the gametic correlation between paternal and maternal effects was distinctly different between the two populations. Specifically, in the Retinto population, the gametic correlation showed a positive skew with posterior probabilities of 0.78 for the TNB and 0.80 for the NBA. On the other hand, the Entrepelado population showed a posterior probability of a positive gametic correlation between paternal and maternal effects of approximately 0.50. The differences in the shape of the posterior distribution of the gametic correlations between paternal and maternal effects observed in the two varieties may account for the distinct performance outcomes observed in the reciprocal crosses.

Entrepreneurs' work experiences and the growth of Chinese private firms during the transition towards a market economy

This work sheds some light on the influence that entrepreneurs' previous work experiences exerted on the growth performance of Chinese private firms after the privatization reform in the early 2000s. Focusing on a representative and large sample of private firms, the analysis finds evidence of an inferior performance of the companies run by entrepreneurs with past top management experience in state-owned companies, while the superior performance of the companies run by entrepreneurs with prior technical experience. The study investigates specific theory-driven mechanisms through which different experiences impact firm performance, including time allocation and R&D management strategy. Upper echelon theory and imprinting effects appear relevant, and local institutions, political and economic uncertainty, and entrepreneurs' self-perception act as moderating factors. We deal with potential endogeneity issues with propensity score matching and two-stage least squares regression. These findings provide novel evidence on underexplored aspects of the ongoing privatization process in China and other economies in transition.

Founder Commercial Imprint Interacts with Strategic Orientations in Affecting Social Enterprise Performance

Many social enterprises have founders who worked in commercial organisations before they engaged in social entrepreneurship, but how that sort of individual-level imprinting exerts an enduring effect on social enterprise performance remains under-examined. Drawing from imprinting theory and the strategic orientation literature, this study examines how and under which conditions social entrepreneurs’ commercial imprint plays a significant role in shaping the performance of their social enterprises. Studying 230 social enterprises in China, this research shows that founders’ most recent for-profit work experience prior to starting their social enterprise has an enduring impact on organisational performance. Moreover, when the founder commercial imprint aligns with the strategic choice that the social enterprise leverages, it creates more benefits for social enterprise performance. This study provides insights into how social entrepreneurs’ career imprints transfer and are adapted to the context of social entrepreneurship. In addition, it enriches the literature by identifying boundary conditions of imprinting effects regarding social enterprise performance.

The imprinting effects of parent firms on the evolution of young spinoff alliance networks

ABSTRACT We tested the drivers of spinoffs’ (that is, new firms started by ex-employees of incumbent firms) alliance network size through the lens of imprinting theory, using a large longitudinal sample of 145 newly founded spinoffs and 3,405 strategic alliances from 2001 to 2014 in the alliance-intensive mining industry. Our results revealed that whereas parent firms’ network positions in terms of size and centrality leave an influential early imprinting effect on spinoffs’ alliance network size, initial partners’ network position leaves an effect through path-dependent forces. Further, our analysis revealed that the parent’s network characteristics can influence the choice of initial partner. We discuss implications for alliance network emergence, spinoffs, and imprinting theory.

Genetic analysis of parent-of-origin effects on growth traits and yearling greasy fleece weight in Raeini Cashmere goat

Pedigree on 7253 individuals and data information on growth traits and greasy yearling fleece weight collected from 1979 to 2013 at the Breeding Station of Raeini Cashmere goat, south-east of Iran, were used for genetic analysis of parent of origin effects for these traits using a gametic relationship matrix. The studied traits included birth weight (BW; 4747 records), weaning weight (WW; 3268 records), six months weight (6 MW; 2855 records), nine months weight (9 MW; 1407 records), yearling weight (YW; 1013 records), and yearling greasy fleece weight (YFW; 1272 records). Models including or excluding imprinting (maternal and paternal) effects were compared by applying Akaike Information Criterion (AIC). Paternal imprinting effects did not influence any of the studied traits. Maternal imprinting effects explained 9%, 6%, 9%, 18%, and 6% of phenotypic variances for WW, 6 MW, 9 MW, YW, and YFW, respectively. By including the maternal imprinting effects in the model used for genetic analysis, direct heritability estimates for WW (from 0.04 to 0.01), 9 MW (0.24–0.15), YW (from 0.31 to 0.14), and YFW (0.18–0.09) were decreased, implying a considerable proportion of the maternal imprinting effects for these traits may overlap with direct additive genetic effects. BW did not influence by imprinting effects. Strong maternal imprinting correlations among body weights at different ages (except for BW) showed that WW, 6 MW, 9 MW, and YW share similar genes with maternally imprinted effects. It means that the imprinting effects of genes are not limited to a specific stage of life and can be active in different stages of growth. Maternal imprinting correlation estimates between YFW and other traits were not statistically significant. Therefore, imprinting effects may be crucial for the genetically evaluating of these traits in the Raeini Cashmere goat.

Loss of Monoallelic Expression of IGF2 in the Adult Liver Via Alternative Promoter Usage and Chromatin Reorganization.

In mammals, genomic imprinting operates via gene silencing mechanisms. Although conservation of the imprinting mechanism at the H19/IGF2 locus has been generally described in pigs, tissue-specific imprinting at the transcript level, monoallelic-to-biallelic conversion, and spatio-temporal chromatin reorganization remain largely uninvestigated. Here, we delineate spatially regulated imprinting of IGF2 transcripts, age-dependent hepatic mono- to biallelic conversion, and reorganization of topologically associating domains at the porcine H19/IGF2 locus for better translation to human and animal research. Whole-genome bisulfite sequencing (WGBS) and RNA sequencing (RNA-seq) of normal and parthenogenetic porcine embryos revealed the paternally hypermethylated H19 differentially methylated region and paternal expression of IGF2. Using a polymorphism-based approach and omics datasets from chromatin immunoprecipitation sequencing (ChIP–seq), whole-genome sequencing (WGS), RNA-seq, and Hi-C, regulation of IGF2 during development was analyzed. Regulatory elements in the liver were distinguished from those in the muscle where the porcine IGF2 transcript was monoallelically expressed. The IGF2 transcript from the liver was biallelically expressed at later developmental stages in both pigs and humans. Chromatin interaction was less frequent in the adult liver compared to the fetal liver and skeletal muscle. The duration of genomic imprinting effects within the H19/IGF2 locus might be reduced in the liver with biallelic conversion through alternative promoter usage and chromatin remodeling. Our integrative omics analyses of genome, epigenome, and transcriptome provided a comprehensive view of imprinting status at the H19/IGF2 cluster.

Quantifying parent-of-origin variation in growth and reproductive traits of Kermani sheep

AbstractRecords on growth and reproductive characteristics collected from 1993 to 2013 at the Breeding Station of Kermani sheep, south-east of Iran, were used for estimating genetic parameters related to parent-of-origin effects. The studied growth traits were birth weight (BWT), weaning weight (WWT), six-month weight (6MW), average daily gain from birth to weaning (ADG1), and average daily gain from weaning to six months of age (ADG2). The reproductive traits were litter size at birth per ewe lambing (LSB), litter size at weaning per ewe lambing (LSW), the sum of litter weight at birth per ewe lambing (LWB), and the sum of litter weight at weaning per ewe lambing (LWW). Models with and without imprinting (maternal and paternal) effects were compared by applying Akaike's information criterion (AIC). Maternal imprinting effects explained 14, 7, 24 and 5% of the phenotypic variances for BWT, WWT, 6MW and ADG1, respectively and their inclusion in the model decreased direct heritability estimates for BWT (from 0.07 to 0.05), WWT (from 0.29 to 0.23), 6MW (from 0.26 to 0.12) and ADG1 (from 0.20 to 0.17). Imprinting effects did not affect ADG2 and studied reproductive traits. The results indicated that a considerable proportion of the maternal imprinting effects overlap with direct additive genetic and maternal effects. Therefore, imprinting effects may be crucial for the genetic assessment of BWT, WWT, 6MW and ADG1 in Kermani sheep.

Subsidiary staffing, location choice, and shareholder rights effectiveness

Institutional differences between countries influence strategic choices and performance of international businesses, but the unintended effects of legal institutions on firm legitimacy have received less attention. We argue that, while minority shareholder rights protection in an investment location does not directly protect shareholder interests abroad, the normative and mimetic effects it has on host country managers can mitigate agency problems. Using Japanese FDI established between 1986 and 2013 we find that (a) subsidiaries established in host countries with higher shareholder rights protection employ a smaller proportion of Japanese expatriates, (b) shareholder rights protection enhances a country’s FDI attractiveness, and (c) that the impacts of shareholder rights protection on expatriate ratio and location attractiveness are stronger when firm ownership is concentrated among exchange-listed firms. This research contributes to the literature on institutional difference in international business, in particular by highlighting the value of studying the imprinting effects of regulations.

Relative contribution of Imprinting, X chromosome and Litter effects to phenotypic variation in economic traits of sheep.

Data on Zandi sheep were analysed to quantify maternal and paternal imprinting, X chromosome and litter effects' contribution to phenotypic variation in birth weight (BW), weaning weight (WW), growth rate (GR), Kleiber ratio (KR), efficiency of growth (EF) and relative growth rate (RGR). To this end, a two-step approach was adopted. In the first step, each trait was analysed with a series of 16 animal models, which were identical for fixed and autosomal additive genetic effects but differed for combinations of maternal permanent environmental, maternal genetic, X chromosome and litter effects. For each trait, the best model was selected by the Akaike information criterion (AIC) and likelihood ratio tests (LRTs). In the second step, three additional models were fitted by adding maternal imprinting, paternal imprinting or both (models 17, 18 and 19) to the best model selected in the first step. Estimators of bias, dispersion and accuracy of breeding values estimated within 19 models with whole, and partial data were used to evaluate how well were the 19 models in estimating breeding values for the animals when their records were masked. For all traits studied, fitting the litter effect led to a better data fit. Also, it resulted in noticeable decreases in residual variance and other maternal variances. For growth traits, models containing the X-linked effects fitted the data substantially better than corresponding models without the X-linked effects. For BW, WW and GR, estimates of X-linked heritability ( ) ranged between 0.09 (GR) and 0.14 (BW). Ignoring X-linked effects from the genetic evaluation model resulted in significant inflated autosomal additive genetic variance. For BW, WW, EF and RGR, models containing the imprinting effects provided a better fit of the data than otherwise identical models. Imprinting effects contributed significantly to the phenotypic variation of these traits in a range between 5% (RGR) and 8% (BW, WW). A sharp decline was observed in autosomal additive genetic variance following including imprinting effects in the model (27% to 40% depending on the trait). The least bias and dispersion, as well as greater accuracies for breeding values of focal animals, were for a model which included imprinting, X-linked and litter effects. It was concluded that imprinting, X-linked and litter effects need to be included in the genetic evaluation models for growth and efficiency-related traits of Zandi lambs.

Imprinting effects of exposure to the Indian independence movement on export intensity of firms

Extending the concept of historical imprinting and organizational learning, we propose that the prior exposure to the Indian independence movement negatively influences the export intensity of firms. Firm-specific characteristics such as business group affiliation and entrepreneurial orientation act as dynamics of amplification and encourage to utilize the organizational learning gained from the historical imprinting. Business group affiliation strengthens the negative relationship between prior exposure to the Indian independence movement and export intensity. Entrepreneurial orientation strengthens the moderating effect of business group affiliation and prior exposure to the Indian independence movement on export intensity resulting in a three-way interaction effect. We test the hypotheses using panel data of 1,817 Indian firm-year observations for 309 firms from 2007 to 2016. We also discuss the theoretical and managerial implications of our findings.

Parental imprinting effects on growth traits and Kleiber ratio in sheep

AbstractThe goal was to study imprinting effects on birth weight (BW), weaning weight (WW), six months weight (W6), pre- and post-weaning growth rate (GRa and GRb), and pre- and post-weaning Kleiber ratio (KRa and KRb) in Baluchi and Makuie sheep breeds. Analyses were done in a two steps process. In the first step, each trait was analysed with a series of 12 univariate animal models, including different combinations of direct and maternal effects and the best model was selected by the AIC criterion. In the second step, three new models were fitted by adding either maternal imprinting effect, paternal imprinting effect, or both of them, respectively, to the best model already selected in the first step, and changes in AIC were monitored. (Co)variances between traits were estimated using bivariate analyses. In Baluchi sheep, for BW, WW and W6, estimates of maternal imprinting heritability (${\boldsymbol h}_{{\boldsymbol mi}}^2$) were 0.12 ± 0.02, 0.08 ± 0.01 and 0.09 ± 0.01, respectively. In Makuie sheep, paternal imprinting heritability (${\boldsymbol h}_{{\boldsymbol pi}}^2$) for BW, WW, W6, GRb and KRb were 0.17 ± 0.05, 0.8 ± 0.05, 0.38 ± 0.10, 0.71 ± 0.15 and 0.65 ± 0.13, respectively. In Baluchi sheep, strong maternal imprinting correlations were observed between BW and WW (0.93 ± 0.05), BW and W6 (0.80 ± 0.07) and WW and W6 (0.91 ± 0.02). In Makuie sheep, paternal imprinting correlations ranged from −0.97 ± 0.33 (BW-GRb) to 0.99 ± 0.47 (GRb-KRb). It was concluded that imprinting effects need to be included in the statistical model to increase the accuracy of genetic evaluation. However, to have accurate and reliable estimates of imprinting effects, the availability of large data sets and deep pedigrees are necessary.

Adiponectin Deficiency Alters Placenta Function but Does Not Affect Fetal Growth in Mice.

Adiponectin administration to pregnant mice decreases nutrient transport and fetal growth. An adiponectin deficiency, on the other hand, as seen in obese women during pregnancy, alters fetal growth; however, the mechanism is unclear. To determine the role of adiponectin on placenta function and fetal growth, we used adiponectin knockout, adiponectin heterozygote that displays reduced adiponectin levels, and wild-type mice on a control diet or high fat/high sucrose (HF/HS) diet. Triglycerides (TGs) in the serum, liver, and placenta were measured using colorimetric assays. Gene expression was measured using quantitative RT-PCR. Adiponectin levels did not affect fetal weight, but it reduced adiponectin levels, increased fetal serum and placenta TG content. Wildtype dams on a HF/HS diet protected the fetuses from fatty acid overload as judged by increased liver TGs in dams and normal serum and liver TG levels in fetuses, while low adiponectin was associated with increased fetal liver TGs. Low maternal adiponectin increased the expression of genes involved in fatty acid transport; Lpl and Cd36 in the placenta. Adiponectin deficiency does not affect fetal growth but induces placental dysfunction and increases fetal TG load, which is enhanced with obesity. This could lead to imprinting effects on the fetus and the development of metabolic dysfunction in the offspring.

Legacy of ideology: The enduring effect of CEOs’ socialist ideological imprint on private firms’ employee-related CSR

The question of how the legacy of ideology has an enduring effect on firms’ decisions remains largely unanswered. Drawing from the imprinting theory and corporate social responsibility (CSR) literature, in this study, we examined when and how chief executive officers’ (CEOs) socialist ideological imprints play an economically significant role in shaping firms' employee-related CSR (E-CSR) in China during its remarkable political transitions. We used data from 4,094 private Chinese firms as our sample and found that the socialist ideological imprints of private firms’ CEOs have a positive impact on the firms’ E-CSR decisions. Moreover, this imprinting effect was found to be more salient when CEOs joined the Communist Party of China before the political transition of Deng Xiaoping’s reform, but was found to be more constrained when CEOs perceived that the level of the guanxi requirements was high. This study sheds new light on how business leaders’ ideological imprints may have an enduring influence on their firms’ discretional CSR choices toward multiple stakeholders, particularly the employees. Moreover, it enhances the imprint literature by spotlighting the newly identified boundary conditions of imprinting effects upon firms’ CSR strategies.

Is there any effect on imprinted genes H19, PEG3, and SNRPN during AOA?

Assisted oocyte activation (AOA) has been proposed as an effective technique to overcome the problem of impaired fertilization after intracytoplasmic sperm injection (ICSI) but the safety of AOA remains a concern. We aimed to investigate if AOA induces imprinting effects on embryos. We used 13 cleavage embryos, nine blastocysts, and eight placentas from 15 patients. The subjects were divided into six groups by tissue type and with or without AOA. The methylation levels of imprinted genes (H19, paternally expressed gene [PEG3] and small nuclear ribonucleoprotein polypeptide N [SNRPN]) were tested by pyrosequencing. We observed different methylation levels among cleavage embryos. The variability was much more remarkable between cleavage embryos than blastocysts and placenta tissues. The methylation levels were especially higher in SNRPN and lower in the H19 gene in AOA embryos than those without AOA. No significant difference was found either among blastocysts or among placenta tissues regardless of AOA. The methylation levels of the three genes in blastocysts were very similar to those in the placenta. Compared to conventional ICSI, AOA changed imprinting methylation rates at H19 and SNRPN in cleavage embryos but not in the blastocyst stage and placenta. We recommend that blastocyst transfer should be considered for patients undergoing AOA during in vitro fertilization.

Is open innovation imprinted on new ventures? The cooperation-inhibiting legacy of authoritarian regimes

Extant research explores the role played by individuals and, in particular, founders in defining open innovation strategies at the firm level. We join this discussion by combining insights from imprinting literature that explores the enduring impact of a founder's personal history, with inputs from literature that stresses the impact of past experience on trust formation. We suggest that founders are less likely to engage in open innovation if their experiences engender a generalized lack of trust. We use a unique database that includes East and West German founders to identify regional differences in activities conducted by authoritarian regimes that could inhibit trust. We find that founders who were exposed to high levels of secret police surveillance in the former socialist German Democratic Republic (GDR) are less likely to engage in interfirm R&D cooperation. We contribute to the literature on open innovation by exploring how a founder's social, political, and cultural backgrounds influence strategic decisions related to open innovation, and to recent imprinting literature by showing that variation in oppressive enforcement practices in authoritarian regimes, such as surveillance activities, can leave an enduring imprint. Our findings complement recent insights on ideological imprinting effects on young firms’ decision-making.

Role of the gut microbiota in airway immunity and host defense against respiratory infections.

Colonization of the intestine with commensal bacteria is known to play a major role in the maintenance of human health. An altered gut microbiome is associated with various ensuing diseases including respiratory diseases. Here, we summarize current knowledge on the impact of the gut microbiota on airway immunity with a focus on consequences for the host defense against respiratory infections. Specific gut commensal microbiota compositions and functions are depicted that mediate protection against respiratory infections with bacterial and viral pathogens. Lastly, we highlight factors that have imprinting effects on the establishment of the gut microbiota early in life and are potentially relevant in the context of respiratory infections. Deepening our understanding of these relationships will allow to exploit the knowledge on how gut microbiome maturation needs to be modulated to ensure lifelong enhanced resistance towards respiratory infections.

Optimization and characterization of electrochemical protein Imprinting on hemispherical porous gold patterns for the detection of trypsin

In this study, protein-imprinted sensors with thin bulk films were electrochemically fabricated on gold-coated quartz crystal electrodes with hemispherical porous gold patterns for detecting trypsin (Trp). The gold patterns were electrodeposited on a polystyrene colloidal monolayer and then rinsed using toluene. For Trp imprinting on the gold patterned electrodes, a thin layer with a poly(o-phenylenediamine) and Trp protein was formed using a cyclic voltammetry method under optimized conditions. In addition, a two-dimensional molecularly imprinted polymer (2D-MIP) film was prepared on a planar gold electrode under the same conditions to compare to the dependence of Trp selective recognition on three-dimensional (3D) thin MIP structure, and each corresponding nonimprinted polymer film were constructed by electropolymerization, in the absence of Trp template, to compare molecular imprinting effects. The sensing properties of Trp imprinted sensors were investigated using electrochemical, such as cyclic voltammetry and electrochemical impedance spectroscopy, and microgravimetric methods to confirm the sensitivity and selectivity of MIP films. The 3D-MIP films demonstrated a higher imprinting factor (3.51) in 48-μg/mL of Trp concentration than the 2D-MIP film, and the limit of detection was calculated to be 70.9-ng/mL. In addition, the films exhibited higher electrochemical sensing responses due to increased Trp recognition by the effective molecular imprinting over a larger surface area. Thus, the construction of 3D-MIP films for the protein imprinting could provide excellent specificity, faster kinetics, and higher sensitivity for detecting macromolecular proteins than 2D-MIP films.

Impacts of Nutritional Management During Early Postnatal Life on Long-Term Physiological and Productive Responses of Beef Cattle

Effective early postnatal nutritional management is a crucial component of livestock production systems, and nutrient manipulation during this period has been shown to exert long-term consequences on beef cattle growth and physiology. Metabolic imprinting defines these biological responses to a nutritional intervention early in life that permanently alter physiological outcomes later in life. Early weaning has been used to study metabolic imprinting effects, given that it allows for nutritional manipulation of animals at a young age. This practice has been shown to enhance carcass characteristics in feedlot cattle and accelerate reproductive development of females. Another strategy to study the effects of metabolic imprinting without the need for early weaning is to provide supplements via creep feeding. Providing creep feed to nursing cattle has resulted in transient and long-term alterations in cattle metabolism, contributing to increased reproductive performance of developing heifers and enhanced carcass quality of feeder cattle. Collectively, results described herein demonstrate nutrient manipulation during early postnatal life exerts long-term consequences on beef cattle productivity and may be a strategy to optimize production efficiency in beef cattle systems.

Migrating Family Owners and Strategic Change Performance: Unpacking the Imprinting Effects

The U.S. is an attractive melting pot for immigrants and has some of the highest domestic migration rates. A notable migration group is family business owners whose first-generation wealth makers business headquarters originated from other regions or countries. These businesses have migrated from their home headquarters, exposing their family businesses to the imprinting effects of the family migration history. This history represents an essential aspect of the family business that can influence how the family responds to environmental changes and informs their strategic performance. Using panel data from 3,369 family-firm-year observations to examine how the migration history of the family business influenced the firm’s strategic performance. Our results indicate that the family migration history of family blockholders influences a firm’s strategic performance through family governance characteristics and industry environmental uncertainty. These results illustrate how the family migration history influences the firm’s adaption and imprints onto the organization, influencing future strategic changes.

Historical Government Regulations and Firms’ Innovation Input

I argue that founding and initial public offering (IPO) are sensitive periods when organizations imprinted by their environment. Stronger government interventions during these sensitive periods imprint firms with a more diversified research and development (R&D) structure and a focus that downplays competition and highlights government intervention, which in turn discourage R&D investment. Moreover, the subsequent sensitive period, IPO, modifies imprinting effects formed during founding. Further, founding is a stronger sensitive period than IPO regarding the magnitude of imprinting effect. I test the regulatory imprinting of (1) U.S. firms based on industry and (2) worldwide firms based on location and find supportive evidence. My research contributes to imprinting theory by (1) showing potential mechanisms through which government interventions in history affect firms’ R&D and (2) articulating organizational sensitive periods in terms of imprinting dynamics and heterogeneity.