Imprinted Fluorescence Sensor Research Articles

A smart imprinted fluorescence sensor of biomass carbon dots based on magnetic covalent organic frameworks for real-time and sensitive detection of 4-nitrophenol in food

Developing sensitive and portable methods for detecting 4-nitrophenol (4-NP) in food is important for ensuring food safety. Herein, a novel detection system using magnetic covalent organic frameworks (MCOFs) combined with biomass carbon dots (CDs) embedded in molecularly imprinted polymers (CDs-MFMIPs) has been designed specifically for detecting 4-NP in food. The biomass CDs synthesized from green persimmons serve as the fluorescent source, while the MCOFs enhance the response signals. The synergistic effect of the fluorescence response from the biomass CDs and the specificity of imprinting sites enable excellent 4-NP detection. This ultrasensitive fluorescence probe exhibits rapid response, excellent selectivity, magnetic properties, and a wide linear range of 0.05–50 μM, with a low detection limit of 17.44 nM. Furthermore, the smartphone-assisted sensor allows for convenient, visual, and precise 4-NP detection. This approach offers a reliable method and promising applications for onsite detection of 4-NP in food.

Design and controlled synthesis of molecularly imprinted fluorescence sensor supported by multifunctional magnetic covalent organic framework: Efficient detection of clofibric acid in the environment

While some studies have explored magnetic covalent organic frameworks (MCOFs) for fluorescence sensing, substantial research gaps remain, especially in integrating molecular imprinting technology (MIT). In this study, superparamagnetic Fe3O4 cores were modified, and a monomer-mediated in situ growth strategy was used to synthesize uniform, multifunctional MCOFs nanoparticles. Subsequently, molecularly imprinted polymers (MIPs) were synthesized via a sol-gel method and deposited on MCOFs, forming MCOFs-supported molecularly imprinted fluorescent sensors (MCOFs@MIPs) for trace analysis of clofibric acid (CLF). This was the first application of molecularly imprinted fluorescence sensing technology for detecting CLF. MCOFs@MIPs combined the superparamagnetism of Fe3O4, the stability and signal amplification of COFs, and the selectivity of MIPs for CLF. Studies demonstrated that MCOFs@MIPs had a maximum fluorescence response in isopropanol, with an optimal dosage of 0.39 mg/mL, an imprinting factor (IF) of 2.73, a detection limit of 94 nM, and a range of 0 ∼ 300 µM. Characterizations revealed that the fluorescence quenching of MCOFs@MIPs was based on a photoinduced electron transfer (PET) mechanism, with MCOFs@MIPs as donors and CLF as acceptors. MCOFs@MIPs were used to detect CLF in actual samples from food, water, agricultural products, cosmetics, and human metabolites. The detection results were consistent with those from high-performance liquid chromatography (HPLC). Additionally, the recoveries of MCOFs@MIPs for actual samples ranged from 94.4 % to 100.7 %, demonstrating exceptional reliability, reusability, and accuracy.

A highly efficient molecularly imprinted fluorescence sensor for selective and sensitive detection of tetracycline antibiotic residues in pork

Tetracyclines (TCs) residues in animal-based foods may cause consumer poisoning or allergic reactions, as well as resistance of pathogenic microorganisms. Therefore, a method to accurately monitor TCs is urgently needed. Here, a sensitive and greenificated molecularly imprinted fluorescent sensor (CDs@MIP) for detecting TCs residues using non-covalent imprinting binding method has been synthesized in a simple and safe manner. The sensor was demonstrated to contain specific adsorption sites and high adsorption capacity by static adsorption test results and Freundlich and Langmuir model analyses, and the maximum adsorption capacity was 45.8 mg/g. Moreover, the sensor exhibited intense blue-green fluorescence under UV light, and the fluorescence was gradually quenched as TCs was adsorbed to achieve the detection. The fluorescent spectrum experiment demonstrated that the prepared sensor has good recognition and detection performance for TCs with a linear range of 0.02–50 mg/L and a detection limit of 0.18 μg/L. Furthermore, This sensor is recyclable and can be used to determine TCs content in pork with a recovery rate of 98 %–108 %. Therefore, the constructed sensor provides a new convenient strategy for detecting TCs residues.

A Molecularly Imprinted Fluorescence Sensor Based on Upconversion-Nanoparticle-Grafted Covalent Organic Frameworks for Specific Detection of Methimazole

Rapid detection and sensitive analysis of MMZ is of great importance for food safety. Herein, a fluorescent molecularly imprinted sensor based on upconversion nanoparticles (UCNPs) grafted onto covalent organic frameworks (COFs) was designed for the detection of MMZ. COFs with a high specific surface area and excellent affinity serve as substrates for grafting of UCNPs, which can inhibit the aggregation burst of UCNPs and improve the mass transfer rate of the sensor. Through a series of characterizations, it was found that the proposed UCNP-grafted COFs@MIP-based sensor had good optical stability, high adsorption efficiency, strong anti-interference ability, and high sensitivity owing to the integration of the advantages of UCNPs, COFs and MIPs. Under the optimal conditions, a good linear relationship was presented between the fluorescence intensity of UCNP-grafted COFs@MIPs and the methimazole concentration in the range of 0.05–3 mg L−1, and the detection limit was 3 μg L−1. The as-prepared UCNP-grafted COFs@MIPs were successfully applied for the detection of MMZ in actual samples, and the results were relevant with those determined by high-performance liquid chromatography. The sensor has good sensitivity, reusability, and high selectivity, which are highly valuable in the rapid analysis and detection of food safety.

A ratiometric fluorescence imprinted sensor based on N-CDs and metal–organic frameworks for visual smart detection of malathion

Sensitive and rapid detection of pesticide residues in food is essential for human safety. A ratiometric imprinted fluorescence sensor N-CDs@Eu-MOF@MIP (BR@MIP) was constructed to sensitively detect malathion (Mal). Europium-based metal organic frameworks (Eu-MOF) were used as supporters to improve the sensitivity of the BR@MIP. N-doped carbon dots (N-CDs) were used as fluorescent source to produce fluorescent signal. A linear relationship between the concentration of Mal and the fluorescence response of the sensor was found in the Mal concentration range of 1–10 μM with a limit of detection (LOD) of 0.05 μM. Furthermore, the sensor was successfully applied for the detection of Mal in lettuce, tap water, and soil samples, with recoveries in the range of 93.0 % − 99.3 %. Additionally, smartphone-based sensors were used to detect Mal in simulated real samples. Thus, the construction of ratiometric imprinted fluorescence sensor has provided a good strategy for the detection of Mal.

A Smartphone-Integrated Molecularly Imprinted Fluorescence Sensor for Visual Detection of Chlortetracycline Based on N,P-Codoped Carbon Dots Decorated Iron-Based Metal-Organic Frameworks.

The residue of chlortetracycline is potentially hazardous to human health; it is meaningful to exploit a portable, rapid, sensitive, and selective method for detection of chlortetracycline (CTC). In this study, a novel fluorescence bionic sensing probe (NH2-MIL-53&N,P-CDs@MIP) was successfully prepared based on the nitrogen and phosphorus codoped carbon dots decorated iron-based metal-organic frameworks combining with molecular imprinted polymer for the detection of CTC. A fluorescence intensity-responsive "on-off" detection of CTC on account of the inner-filter effect (IFE) was achieved by NH2-MIL-53&N,P-CDs@MIP. Under the optimal conditions, the fluorescence quenching degree of NH2-MIL-53&N,P-CDs@MIP presented a good linear relationship with the CTC concentration in the range 0.06-30 μg mL-1 and the limit of detection (LOD) was 0.019 μg mL-1. The fluorescent probe was applied to detect CTC in milk samples, and experimental results showed a good recovery rate (88.73%-96.28%). Additionally, a smartphone-integrated fluorescence sensing device based on NH2-MIL-53&N,P-CDs@MIP was exploited to replace the expensive and bulky fluorescence spectrophotometer for quantitative determination of CTC with the LOD of 0.033 μg mL-1. The sensing system showed high selectivity, strong stability, high specificity, and portability, which provide a great strategy for the quantitative detection of antibiotic residue.

Surface-molecularly imprinted ratiometric fluorescence sensor for fast, sensitive and selective determination of rhodamine 6G

Rhodamine 6G (Rh6G) is a classic dye that has been banned as a food additive due to its harm to human bodies. The illegal addition of Rh6G in foods should be strictly monitored. In this study, a surface-molecularly imprinted fluorescence sensor (TPP-SMIPs) for Rh6G detection was prepared on SiO2 using an aggregation-induced emission molecule as a fluorescence signal part. TPP-SMIPs exhibited ratiometric response to Rh6G. Favorable linearity between the fluorescence intensity ratios and Rh6G was obtained in the range of 0–10.0 μmol/L. Better site accessibility and higher mass transfer rate were achieved in TPP-SMIPs than in non-surface imprinted polymers. High sensitivity with a low detection limit (2.82 nmol/L) and excellent selectivity were also realized in TPP-SMIPs. Its applications in real sample detection showed satisfactory recoveries and relative standard deviations. This study demonstrated that this sensor can provide fast, sensitive and selective determination to Rh6G in real samples. This work not only reported a new detection method for rhodamine dyes, but also provided a universal strategy for the design of high-performance molecularly imprinted fluorescence sensors.

A smartphone-assisted dual-response imprinted fluorescence sensor based on UiO-66 and quantum dots for detecting bovine hemoglobin

Establishment of precise, visual and on-site fluorescence detection system for proteins is of great importance in the field of biology and medicine. Herein, a novel dual-emission molecularly imprinted fluorescence sensor was designed for detecting bovine hemoglobin (BHb) based on silicon quantum dots (Si QDs), red CdTe QDs (r-CdTe QDs) and zirconium-based metal–organic framework (UiO-66). In this system, the porous UiO-66, served as supporter, endowed imprinted polymer (Si QDs/r-CdTe@UiO-66@MIP) with more specific binding sites and boosted mass transfer rate. Si QDs and r-CdTe QDs were used as optosensing centers and provided two-emission signals at 438 nm and 658 nm. Under the optimal detection conditions, Si QDs/r-CdTe@UiO-66@MIP exhibited good linear relationship to BHb in the range of 0.1–10 µM with a low detection limit (32 nM). Meanwhile, given by outstanding performances of Si QDs/r-CdTe@UiO-66@MIP, a home-made sensing device based on smartphone incorporating nanosensor was designed for the analysis of BHb. The portable optical device exhibited obvious color changes from blue to yellow, and provided visual detection. Significantly, the smartphone sensing system was successfully applied to analysis of BHb in simulated actual samples (urine and human serum), indicating that the smartphone-assisted fluorescence platform had great application potential for on-site monitoring BHb.

A novel smartphone-integrated binary-emission molecularly imprinted fluorescence sensor embedded with MIL-101(Cr) for sensitive and real-time detection of protein

Aiming for precise, real-time, and on-site analysis of proteins, an innovative binary-emission fluorescence imprinted polymer was designed by sol-gel method after mixing MIL-101(Cr), green CdTe (g-CdTe) and red CdTe (r-CdTe) for detection of protein. In this proposal, MIL-101(Cr), as a favorable supporter, provided high surface area and porosity for imprinting sites, which ameliorated the transfer rate and the sensitivity of the nanosensor. And g-CdTe and r-CdTe were served as signal transduction for dual-emission response. Based on strengthened recognition reaction between high-affinity imprinting sites and protein, the fluorescence intensities of g-CdTe and r-CdTe yielded conspicuous two responses at 528 nm and 634 nm for protein under the excitation of 350 nm. The cytochrome c (Cyt c) and trypsin were served as model proteins to verify the generality of strategy. Given prominent merits of MIL-101(Cr), g-CdTe/r-CdTe@MIL-101(Cr)@MIP exhibited good linear range of 1–30 μM for Cyt c and 0.15–4 μM for trypsin, and the limit of detection were 0.13 μM and 0.014 μM, respectively. Significantly, an unsophisticated smartphone-based sensing device was developed by integrating g-CdTe/r-CdTe@MIL-101(Cr)@MIP with a 3D printing portable device to obtain precise on-site results. As expected, this portable platform was successfully applied for monitoring Cyt c and trypsin with a detection limit of 0.71 μM and 0.026 μM, respectively. These results indicated this dual-response molecularly imprinted fluorescence senor based on smartphone provided promising perspectives on futural on-site protein analysis.

A molecularly imprinted fluorescence sensor for sensitive detection of tetracycline using nitrogen-doped carbon dots-embedded zinc-based metal-organic frameworks as signal-amplifying tags

Tetracycline (TC) residues not only endanger human health, but also are detrimental to the sustainable development of aquaculture and animal husbandry. Herein, a novel fluorescence sensor with high sensitivity and selectivity was developed based on nitrogen-doped carbon dots embedded in zinc-based metal-organic frameworks and incorporating molecularly imprinted polymer (ZIF-8&N-CDs@MIP). The physical and chemical properties of the ZIF-8&N-CDs@MIP had been characterized by SEM, TEM, FTIR, XRD, BET, TGA, etc. Under optimal conditions, the limit of detection (LOD) of the novel sensor was 0.045 μg mL−1 with the concentration of TC in the range of 0.1–4.0 μg mL−1. In addition, the prepared imprinted polymers showed superior adsorption selectivity to tetracycline compared with non-imprinted polymers, and the quenching mechanism of ZIF-8&N-CDs@MIP was demonstrated to be attributed to the inner filter effect (IFE). This work provided an effective and reliable method for the specific detection of tetracycline and was successfully applied in milk and egg samples with satisfactory recoveries (80.67–95.22%).

A molecular imprinted fluorescence sensor based on carbon quantum dots for selective detection of 4-nitrophenol in aqueous environments

P-nitrophenol (4-NP) is the most persistent and highly toxic species among nitrophenol. In this work, a novel fluorescent probe for the detection of 4-NP in aqueous environment was constructed by combining the carbon dots (CQDs) with excellent optical properties and the molecularly imprinted polymer (MIP) with favorable selectivity. The CQDs were synthesized by hydrothermal method using citric acid hydrate as carbon source and o-phenylenediamine as surface modifier, then the molecularly imprinted polymers coating on the CQDs (MIP@CQDs) were obtained by sol-gel imprinting process. The fluorescence quenching of MIP@CQDs is the results of internal filtration effect and dynamic quenching when they encounter with 4-NP. The probe is suitable for the quantitative detection of trace 4-NP in actual aqueous samples, such as tap water, wastewater and seawater, with satisfying recoveries from 95.1 % to 107.8 %, wide detection linear ranges between 0 and 144 μmol/L, low detection limit of 0.41 μmol/L and high selectivity. The detection results are consistent with those of the HPLC method. This work provides a simple, rapid and effective fluorescent detection method for trace 4-NP in aqueous environment.

A highly efficient molecularly imprinted fluorescence sensor for assessing whole wheat grains by the rapid and sensitive detection of alkylresorcinols

To differentiate whole wheat foods from refined wheat foods is still challenging grain industry and confusing consumers. Alkylresorcinols (ARs), as biomarkers of whole wheat grains, can serve for assessing the authenticity of whole wheat foods. Herein, a highly efficient fluorescence sensing platform (CDs@MIP) for rapid and sensitive analysis of ARs was explored, using carbon dots (CDs) as fluorophores and 5-heneicosylresorcinol (C21:0 AR) as template molecules embedded in a molecularly imprinted polymer (MIP) coating. Benefiting from the specific cavities in the probe and a photo-induced electron transfer effect, the fluorescence intensity of CDs@MIP was significantly quenched in the presence of C21:0 AR, exhibiting a superior binding efficiency and selectivity. As a result, the fabricated optical sensor delivered a wide linear range of C21:0 AR from 0.015 to 60 μg mL−1 with an ultralow detection limit of 4 ng mL−1. It was noteworthy that the sensor was successfully applied for the rapid detection of C21:0 AR in commercial whole-wheat foods as well as visualization analysis on the test paper, comprehensively validating the practicality and efficacy of CDs@MIP based fluorescence assay. The study provides a rapid and sensitive detection method of C21:0 AR, paving a new way for guiding grain industry to effectively qualify the authenticity and to quantify the content of whole wheat in wheat-based foods.

One-pot synthesis of ternary-emission molecularly imprinted fluorescence sensor based on metal–organic framework for visual detection of chloramphenicol

In this paper, a ternary-emission fluorescence imprinted polymer was one-pot synthesized by sol–gel method after mixing luminescence metal organic framework, green CdTe and near infrared red CdTe for visual detection of chloramphenicol in food. The ternary-emission fluorescence imprinted sensor showed wider linear range within concentration of 10 pM–0.5 nM and 0.5 nM–4.5 nM, with rapid response time of 3 min and the lower detection limit of 3.8 pM toward chloramphenicol. Meanwhile, NH2-UiO-66 improved the fluorescence sensitivity and response speed, the near-infrared CdTe enhanced the anti-interference ability of the imprinted sensor. Compared with the traditional single-emission and dual-emission fluorescence imprinted sensors, the ternary-emission imprinted sensor provided richer color changes from yellowish green to apricot to orange-salmon to amaranth to purple to final blue. It was applied to detect trace chloramphenicol in food with the recoveries of 98.2–101.2 %, which provided a new way for rapid visual detection of chloramphenicol.

Mesoporous silica imprinted carbon dots for the selective fluorescent detection of triclosan

A molecularly imprinted fluorescence sensor built as a mesoporous structured silica imprinted layer on the surface of carbon dots (CDs@m-MIP) was employed for the selective detection of triclosan (TRI). The fluorescence of this CDs@m-MIP was affected sensitively and selectively by TRI via an electron transfer-induced fluorescence quenching mechanism with a detection limit of TRI at 1.08 nM (range 1.72–138 nM) under the optimum setup (e.g., pH, response time, and CDs@m-MIP dose). This approach was used successfully to detect TRI in real water samples (e.g., sewage, river, and tap water). The recoveries of TRI were satisfactory in spiked river and tap water (in 94.7–99.5 %). The outcome of this research is thus expected to help develop highly efficient fluorescent sensing systems towards diverse hazardous compounds including TRI.

Mesoporous silica-based molecularly imprinted fluorescence sensor for the ultrafast and sensitive recognition of oxytetracycline

Molecularly imprinted sensor has been a common chemical sensor for the selective detection of target. Yet, the rapid detection of target was one of the remaining problems, which needs to be diligently improved. Herein, a molecularly imprinted mesoporous silicon material (N-CDs@mMIPs) was successfully prepared for the rapid, sensitive and selective detection of oxytetracycline (OTC). Non-toxic nitrogen-doped carbon quantum dots (N-CDs) with high fluorescence performance was chosen as a detectable fluorescence signal, OTC was the target analysis, 3-Aminopropyltriethoxysilane (APTES) as the functional monomer, tetraethyl silicate (TEOS) as the crossing link agent and cetyl trimethyl ammonium bromide (CTAB) as the pore-foaming agent. N-CDs@mMIPs was synthesized by the removal of CTAB and OTC. Under the optimal test conditions, the resultant N-CDs@mMIPs showed a faster response time within 0.5 min (30 s) and a lower detection limit of 0.035 μmol·L−1 compared with molecularly imprinted sensor without mesoporous structure (N-CDs@MIPs) and other molecularly imprinted sensors. Consequently, N-CDs@mMIPs was applied for the detection of OTC in milk and honey, satisfied recovery rates of 99.80 %–100.60 % and 99.75 %–102.10 % were obtained respectively. In general, the pore structure in mesoporous silicon accelerated the mass transfer rate between target and fluorescent substance, which greatly shortened the detection time of N-CDs@mMIPs to OTC. Furthermore, the pore structure in mesoporous silicon could effectively avoid the self-aggregation of N-CDs, thus improved the sensitivity of N-CDs@mMIPs.

A UCMPs@MIL-100 based thermo-sensitive molecularly imprinted fluorescence sensor for effective detection of \u03b2-lactoglobulin allergen in milk products

In this study, a thermo-sensitive molecularly imprinted fluorescence sensor was developed for the specific detection of β-Lactoglobulin (β-LG) allergen in milk products. The metal–organic frameworks (MIL-100) with a high specific surface area was coated on the surface of upconversion micro-particles (UCMPs). As the core, an imprinted polymer layer allowing for swelling and shrinking with response to temperature was prepared, which exhibited high adsorption and mass transfer capabilities for β-LG allergen. The fluorescence intensity of UCMPs@MIL-100@MIP decreased linearly with the concentration of β-LG in the range of 0.1–0.8 mg mL−1, and the limit of detection was 0.043 mg mL−1. The imprinting factor reached 3.415, which indicated that excellent specificity of the UCMPs@MIL-100@MIP for β-LG allergen. In the analysis of β-LG allergen in actual milk samples, the proposed UCMPs@MIL-100@MIP fluorescence sensor produced reliable and accurate results (recovery: 86.0–98.4%, RSD: 2.8–6.8%), closely related to the results of standard HPLC method (correlation coefficient: 0.9949), indicating that its feasibility in the detection of β-LG allergen.Graphical

Application of novel quantum dot-based molecularly imprinted fluorescence sensor in rapid detection

作为一种新型荧光纳米材料,量子点具有十分优异的光学特性,是分析化学、生物科学、医学等领域研究的热点标记材料。分子印迹聚合物是能够进行特异性识别和选择性吸附的“仿生”材料,它易于制备且具有较好的重现性和稳定性,因而分子印迹技术已成为具有广阔应用前景的识别技术。量子点基分子印迹荧光传感器结合了量子点和分子印迹技术的优势,由于其高选择性和高灵敏度,在环境监测、食品检测、生物分析等领域得到快速发展。但该传感器在应用中也还存在亲水性不足、识别单一、便携性不足等问题。该文引用了近5年来发表在American Chemical Society、Elsevier等数据库约20篇相关文献,对量子点基分子印迹荧光传感器的构建及该传感器在快速检测分析痕量物质中的应用进展进行了综述。首先根据荧光光谱图中发射峰个数的不同分别介绍了3种量子点基分子印迹荧光传感器的类型及相关识别机理,其次根据待测物的不同归纳介绍了近五年来该传感器在离子、有机小分子、生物大分子等检测分析中的最新研究进展,最后对当前该传感器在制备及应用中仍存在的问题进行了总结并对其发展趋势进行了展望。

Construction and Application of Molecularly Imprinted Fluorescence Sensor

Construction and Application of Molecularly Imprinted Fluorescence Sensor

Ultrasensitive molecularly imprinted fluorescence sensor for simultaneous determination of CA125 and CA15-3 in human serum and OVCAR-3 and MCF-7 cells lines using Cd and Ni nanoclusters as new emitters.

In the clinical diagnosis of tumors, a single-marker immunoassay may lead to false results. Thus there is a need for an effective and valid method for the simultaneous measurement of multiple tumor markers. In this work, an efficient fluorescence immunosensor for the simultaneous measurement of CA125 and CA15-3 tumor markers was fabricated by utilizing the high selectivity of magnetic molecularly imprinted polymers (MMIPs) and the high sensitivity of a fluorescence (FL) method. Ni nanoclusters (Ni NCs) and noble Cd nanoclusters (Cd NCs) were introduced as efficient and economic emitters, and magnetic graphene oxide (GO-Fe3O4) was applied as a support material for surface molecularly imprinted polymers. Under the most favorable experimental conditions, the fluorescence intensity of the Cd NCs and Ni NCs gradually increased with increasing concentration of CA125 and CA15-3 antigens at a range of 0.0005-40UmL-1, respectively, with a limit of detection (LOD) of 50 μU mL-1. The developed method had excellent properties including a broad linear range, good reproducibility, and simple operation for the clinical diagnosis of CA 125 and CA 15-3 tumor markers. This molecularly imprinted fluorescence sensor has the potential to be an effective clinical tool for the timely screening of breast cancer in human serum samples and OVCAR-3 and MCF-7 cell lines, and can be applied in clinical diagnostics.

An eco-friendly near infrared fluorescence molecularly imprinted sensor based on zeolite imidazolate framework-8 for rapid determination of trace trypsin

This report depicts an environment-friendly molecularly imprinted fluorescence sensor for rapid determination of trypsin based on new near infrared (NIR) fluorescence carbon dots and metal–organic frameworks. The novel NIR fluorescence carbon dots were facilely synthesized from green plants by one-step solvothermal treatment, and the imprinted sensor was prepared by sol–gel under mild condition. The imprinted NIR fluorescence sensor was characterized by Fourier-transform infrared spectrometry, energy disperse spectroscopy, scanning electron microscopy, X-ray diffraction and transmission electron microscopy. The results showed the introduction of zeolite imidazolate framework-8 can improve the fluorescence response sensitivity and response speed. Under optimized conditions, the fluorescence quenching of the imprinted NIR fluorescence sensor was linear relationship with the increasing trypsin concentration in the range of 0.05–9.0 ng/L with a detection limit of 8.8 pg/L. The developed imprinted fluorescence sensor can selectively recognize trypsin in 1.5 min, which was applied to rapidly and sensitively detect trypsin in serum and urine samples successfully with the recoveries of 95.3–104.1% and 97.7–105.2%, respectively. The as-prepared imprinted fluorescence sensor provides a viable and eco-friendly method for rapidly sensitive detection of trace bio-macromolecule in complex samples.