Important Disease Research Articles

Investigation of Viral, Bacterial and Parasitic Zoonotic Diseases in Rodents in Turkey.

Rodents are reservoir hosts for zoonotic pathogens that cause tropical diseases, many of which have been overlooked. The aim of this study was to investigate the presence of viral lymphocytic choriomeningitis and hantavirus infections, bacterial tularaemia and leptospirosis, and parasitic leishmaniasis and toxoplasmosis in rodents that are likely to carry and spread zoonotic agents, by using molecular methods. A total of 498 voles collected from 20 counties of Erzurum province. Conventional PCR was used for pathogen search. PCR-positive samples were subjected to sequence analysis. Hantavirus (4.8%, 24/498) and tularaemia (0.8%, 4/498) positivity were detected. However, no positivity was detected for other selected pathogens. Rodents, which are pathogen carriers and potential risk factors, are thought to may act as reservoirs for hantavirus and tularaemia in the study area. A preliminary study has been carried out at the point of detection of these diseases of global importance. The extent of the distribution of the infections, alternative hosts and the consequences of human exposure needs to be clarified through further studies.

Detection of novel pathogenic variants of Fusarium oxysporum f. sp. lactucae in California

AbstractFusarium wilt, caused by the soilborne fungal pathogen Fusarium oxysporum f. sp. lactucae (FOL), is an economically important disease of lettuce (Lactuca sativa) throughout the world. Four pathogenic races of FOL are reported, though only race 1 is known to exist in the United States. Recently, Californian lettuce growers have expressed increasing concern about Fusarium wilt, as some race 1‐resistant cultivars have exhibited susceptibility, and some susceptible cultivars have displayed reduced disease severity. To determine whether such changes in disease patterns are responses to potentially novel variants, we collected infected plants from commercial fields in the Salinas Valley and Santa Maria regions, isolated the fungus, and conducted a series of pathogenicity tests using a set of FOL race differential cultivars over 2 years (2022 and 2023) in controlled conditions. Pathogenicity tests revealed two new FOL variants that elicited novel disease reaction patterns on the set of differential cultivars that have not been previously described. Isolates Fol621 and Fol621s were less aggressive on race 1‐susceptible Banchu Red Fire, designated Variant‐1. Isolate VSP‐0916 incited severe Fusarium wilt on race 1‐resistant Costa Rica No. 4, designated Variant‐2. Moreover, VSP‐0916 exhibited high aggressiveness and the ability to induce disease in other race 1‐resistant cultivars. This study is the first documentation, to our knowledge, of the occurrence of FOL‐inciting Fusarium wilt on race 1‐resistant lettuce in the United States. Our work provides valuable information critical for the development of Fusarium wilt management strategies, including broad‐spectrum resistance breeding efforts against multiple FOL races.

Safety of Hepatitis B Virus Screening in Blood Donors from the Hospital Foundation of Hematology and Hemotherapy of the State of Amazonas (HEMOAM) in the Brazilian Amazon

Background: Hepatitis B is an infectious disease of worldwide importance and of great interest to transfusion medicine. The Amazon region has areas of high endemicity, outlining a worrying scenario for transfusion and epidemiological safety. Objective: To analyze the profiles of serological and molecular markers for HBV of blood donors from HEMOAM. Methods: Blood donors with different patterns of reactivity in serological and molecular screening for HBV were tested for viral load by the qPCR method at the reference center for liver diseases in the state of Amazonas. Results: A total of 230,591 donors were tested, with 3104 (1.34%) found reactive for HBV and 2790 (89.9%) found reactive for isolated anti-HBc. Viral load was not detected in 100% of donors reactive only to HBsAg, while 100% of donors with positive anti-HBc and positive HBsAg or HBV NAT demonstrated a detectable viral load. We also detected one case of occult hepatitis B (0.03%) only with reactive HBV NAT and five donors (0.2%) with positive anti-HBc and HBV NAT. Conclusions: With this result, the great importance of the anti-HBc test for the unsuitability of blood donors was verified, as well as the fundamental introduction of the HBV NAT test in screening for hepatitis B in Brazilian blood banks, as this was the only way to detect the viral infection burden in asymptomatic donors who previously would not be treated, which contributed to the maintenance of the endemicity of hepatitis B in the Brazilian Amazon.

Detection of Legionella spp. in influent wastewater in Kobe City, Japan

ABSTRACT Legionella is an important waterborne pathogen that causes Legionnaires' disease (LD). Several outbreaks associated with wastewater treatment plants (WWTPs) have been reported in recent years; however, the prevalence of Legionella in WWTPs in Japan has rarely been investigated. In this study, we investigated the distribution of Legionella in influent wastewater collected from two WWTPs in Kobe, Japan between April 2023 and March 2024. The concentrations for Legionella in all seasons varied between 104 and 106 copies/100 mL in all seasons. Among the 10 Legionella species detected in the influent wastewater, Legionella pneumophila was the most commonly isolated. Genotyping revealed that pathogenic L. pneumophila strains were widely distributed in the influent wastewater in Japan with genetic diversity. LD is one of the most important infectious diseases during natural disasters. This study highlights the importance of influent wastewater as a potential source of LD in Japan, where natural disasters occur frequently.

Time-dependent simulation of blood flow through an abdominal aorta with iliac arteries.

Atherosclerosis is one of the important diseases of the circulatory system because atherosclerotic plaques cause significant disruption of blood flow. Therefore, it is very important to properly understand these processes and skillfully simulate blood flow. In our work, we consider blood flow through an abdominal aorta with iliac arteries, assuming that the right iliac artery is narrowed by an atherosclerotic lesion. Blood flow is simulated using the laminar, standard and standard models. The obtained results show that despite the use of identical initial conditions, the distribution of velocity flow and wall shear stress depends on the choice of flow simulation model. For the model, we obtain higher values of speed and wall shear stress on atherosclerotic plaque than in the other two models. The laminar and models predict larger areas where reverse blood flow occurs in the area behind the atherosclerotic lesion. This effect is associated with negative wall shear stress. These two models give very similar results. The results obtained by us, and those reported in the literature, indicate that model is the most suitable for blood flow analysis.

Investigating the impact of stochasticity on HIV infection dynamics in CD4+\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$$^{+}$$\\end{document} T cells using a reaction-diffusion model

The disease dynamics affect the human life. When one person is affected with a disease and if it is not treated well, it can weaken the immune system of the body. Human Immunodeficiency Virus (HIV) is a virus that attacks the immune system, of the body which is the defense line against diseases. If it is not treated well then HIV progresses to its advanced stages and it is known as Acquired Immunodeficiency Syndrome (AIDS). HIV is typically a disease that can transferred from one person to another in several ways such as through blood, breastfeeding, sharing needles or syringes, and many others. So, the need of the hour is to consider such important disease dynamics and that will help mankind to save them from such severe disease. For the said purpose the reaction-diffusion HIV CD4+\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$$^{+}$$\\end{document} T cell model with drug therapy under the stochastic environment is considered. The underlying model is numerically investigated with two time-efficient schemes and the effects of various parameters used in the model are analyzed and explained in a real-life scenario. Additionally, the obtained results will help the decision-makers to avoid such diseases. The random version of the HIV model is numerically investigated under the influence of time noise in Ito^\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$${\\hat{o}}$$\\end{document} sense. The proposed stochastic backward Euler (SBE) scheme and proposed stochastic Implicit finite difference (SIFD) scheme are developed for the computational study of the underlying model. The consistency of the schemes is proven in the mean square sense and the given system of equations is compatible with both schemes. The stability analysis proves that both schemes and schemes are unconditionally stable. The given system of equations has two equilibria, one is disease-free equilibrium (DFE) and the other is endemic equilibrium. The simulations are drawn for the different values of the parameters. The proposed SBE scheme showed the convergent behavior towards the equilibria for the given values of the parameters but also showed negative behavior that is not biological. The proposed SIFD scheme showed better results as compared with the stochastic SBE scheme. This scheme has convergent and positive behavior towards the equilibria points for the given values of the parameters. The effect of various parameters is also analyzed. Simulations are drawn to evaluate the efficacy of the schemes.

Evaluation of Bioagents against Stemphylium vesicarium Inducing Stemphylium Blight in Onions (Allium cepa L.)

Stemphylium blight of onion incited by S. vesicarium is one of the economically important diseases, causing severe yield losses in onion crop. The present investigation was carried out to evaluate efficacy of different bioagents against S. vesicarium under in vitro conditions. The experiment was laid out in a Completely Randomized Design (CRD) with three replications per treatment. Five fungal biocontrol agents viz., Trichoderma harzianum, T. viride, T. hamatum, T. virens and a fungal consortium from MPKV, Rahuri along with two bacterial biocontrol agents namely Pseudomonas fluorescens and Bacillus subtilis were evaluated for their effectiveness against S. vesicarium. The results showed significant variation in the inhibitory effects of bioagents on the mycelial growth of the test pathogen with percent growth inhibition ranging between 53.70 to 85.19 per cent. T. harzianum was reported to be most effective, showing a minimum colony diameter of 13.33 mm with the highest growth inhibition (85.19%) thus reported as superior to all other treatments whereas, Pseudomonas fluorescens was reported with a least percent growth inhibition (53.70%).

An Effective Yak Behavior Classification Model with Improved YOLO-Pose Network Using Yak Skeleton Key Points Images

Yak behavior is a valuable indicator of their welfare and health. Information about important statuses, including fattening, reproductive health, and diseases, can be reflected and monitored through several indicative behavior patterns. In this study, an improved YOLOv7-pose model was developed to detect six yak behavior patterns in real time using labeled yak key-point images. The model was trained using labeled key-point image data of six behavior patterns including walking, feeding, standing, lying, mounting, and eliminative behaviors collected from seventeen 18-month-old yaks for two weeks. There were another four YOLOv7-pose series models trained as comparison methods for yak behavior pattern detection. The improved YOLOv7-pose model achieved the best detection performance with precision, recall, mAP0.5, and mAP0.5:0.95 of 89.9%, 87.7%, 90.4%, and 76.7%, respectively. The limitation of this study is that the YOLOv7-pose model detected behaviors under complex conditions, such as scene variation, subtle leg postures, and different light conditions, with relatively lower precision, which impacts its detection performance. Future developments in yak behavior pattern detection will amplify the simple size of the dataset and will utilize data streams like optical and video streams for real-time yak monitoring. Additionally, the model will be deployed on edge computing devices for large-scale agricultural applications.

Molecular identification and biological characterization of Eimeria columbarum from domestic pigeons (Columba livia domestica) in Guangdong, China

Pigeon coccidiosis caused by Eimeria spp. is an important veterinary disease with a significant economic impact on the pigeon industry. Preventive measures for Eimeria columbarum in pigeons have been hampered by the lack of extensive genetic, morphological, and biological data on the oocysts. In this study, we examined the prevalence and identity of Eimeria spp. in domestic pigeons from seven cities in Guangdong Province, China. Data show that coccidiosis was prevalent in domestic pigeons in Guangdong Province, with an overall Eimeria spp. detection rate of 73.4%. Five Eimeria species were identified, including E. columbarum (73.4%), Eimeria kapotei (25.6%), Eimeria labbeana (19.6%), Eimeria duculai (19.6%), and Eimeria tropicalis (6.7%). We obtained single oocyst-derived lines of the dominant E. columbarum from fecal specimens. E. columbarum oocysts measured 20.06 ± 0.69 μm × 18.63 ± 1.03 μm, and sporocysts measured 10.29 ± 0.82 μm × 85.38 ± 0.46 μm. In infection experiment using obtained E. columbarum isolates, 60-day-old coccidia-free pigeons exhibited a prepatent period of 105 h and patent period of 9–10 days followed by severe diarrhea, depression, anorexia, and emaciation. Endogenous development of the parasite was observed mainly in the cytoplasm of epithelial cells in the duodenum, jejunum, ileum, and rectum. Two generations of meronts developed on days 3 and 4 after infection, respectively, while gamont and gamete developed on day 5 after infection. The morphological, genetic, and biological data are expected to be useful in elucidating the biological characterization of pigeon coccidiosis to develop measures against the treatment and containment of this disease.

Fusarium Species Shifts in Maize Grain as a Response to Climatic Changes in Poland

Maize, along with wheat and rice, is the most important crop for food security. Ear rots caused by Fusarium species are among the most important diseases of maize. The distribution of Fusarium species provides essential epidemiological information for disease management. The effect of weather conditions, climate change and geographic localization on the Fusarium population in Poland was evaluated between 2015 and 2018. Grain samples (n = 233) were collected from hybrids at 16 locations (L1–L16). The differences in altitude between locations ranged from 39 to 243 m above sea level, longitude varied between 15°55′ and 23°12′ E, and latitude spanned from 50°12′ to 54°01′ N. Isolates were identified using molecular techniques. The highest Fusarium species frequency was recorded for 2016 (30.70%) and 2017 (28.18%), and the lowest for 2018 (5.36%). F. verticillioides and F. temperatum were the most frequent. Altitude has an effect on F. vericillioides frequency: F. graminearum showed a negative correlation with both latitude and longitude. In Polish conditions, from silking to harvesting, the number of days with higher precipitation and lower temperatures is associated with an increased frequency of F. verticillioides, F. temperatum, F. graminearum and F. avenaceum. This suggests that the Fusarium presence in Poland is significantly influenced not only by climate change but also by extreme weather changes.

Prurigo chronica multiformis: Expert consensus of the Japanese Dermatological Association.

Prurigo chronica multiformis is a commonly used diagnostic designation for a peculiar subtype of prurigo in Japan, although the disease entity might not be well-recognized in other countries. Experts approved by the Japanese Dermatological Association attempted to build a common consensus on prurigo chronica multiformis, agreeing that it is a unique and important disease entity in elderly patients. Skin lesions are characterized by intensely pruritic, edematous, urticarial papules, or small macules, which gradually become solid papules/small nodules. The papules tend to aggregate and occasionally coalesce into polygonal lichenified plaques. The most commonly affected sites are the lower abdomen and lower back, although the chest, thighs, and upper back might also be involved. Common histopathological features of prurigo chronica multiformis include infiltration of lymphocytes and eosinophils in the upper dermis, with minimal epidermal changes. Basophil infiltration is also observed. The epidemiological incidence, differences in clinical manifestations by geographical location, and disease placement among other forms of prurigo and/or related skin diseases need to be further elucidated. Dermatologists should be aware of the clinical characteristics of prurigo chronica multiformis.

Isolation and pathogenicity of a fowl adenovirus 8b (FAdV-8b) strain in Cherry Valley ducks

ABSTRACT Inclusion body hepatitis (IBH) is an economically important viral disease primarily affecting the poultry industry. In this study, we isolated a strain of FAdV-8b (strain SDYT) from naturally infected ducks and the hexon and fiber gene sequences were analysed by polymerase chain reaction (PCR) amplification. In order to study the pathogenicity of FAdV-8b in Cherry Valley ducks, we inoculated 10- and 20-day-old ducks with 0.3 ml of FAdV-4 virus (TCID50 of 105.5/0.1 ml) either orally or intramuscularly. Clinical signs, gross lesions and histopathological changes, cytokines, viral load and antibody levels were observed and recorded within 15 days after infection. Pathomorphological investigations revealed that ducks in the experimental group exhibited hepatitis. Histopathology showed multiple organ damage, including serious liver and kidney lesions. Furthermore, elevated levels of inflammatory cytokines and antibodies were noticed, due to the infection and innate immune response. At a later stage of infection, immunosuppression occurred, resulting in decreased levels of cytokines. Determination of viral load showed that the virus was present in several organs, with the highest viral DNA load found in the liver, followed by the kidney. Compared to birds infected orally, the intramuscular group exhibited the highest viral load. In summary, this study increases our understanding of the pathogenicity of FAdV-8b in ducks and establishes a model that will inform antiviral drug testing and vaccine evaluation for IBH, thereby preventing and reducing the spread of IBH in the poultry industry. RESEARCH HIGHLIGHTS A strain (SDYT) of fowl adenovirus 8b (FAdV-8b) was successfully isolated from ducks. Cherry Valley ducks were successfully infected with FAdV-8b. Different routes of infection can lead to duck mortality, more pronounced when birds are injected intramuscularly. FAdV-8b (SDYT) was distributed in various tissues and organs of ducks, causing different degrees of lesions.

Isolation and characterization of a subtype G2c variant of porcine epidemic diarrhea virus that adapts well to cell culture.

Porcine epidemic diarrhea virus (PEDV) causes the third most important disease in the pig industry, after African swine fever and porcine reproductive and respiratory syndrome, and leads to illness or death of the entire litter, causing significant economic losses. In this study, three PEDV strains (HN-1, HN-2, and SC2023) were isolated from swine farms with suspected PEDV infections in Sichuan and Henan provinces. Phylogenetic analysis based on complete S gene sequences showed that all three strains belonged to the G2c subgroup. HN-1 adapted readily to cell culture, grew to a viral titer as high as 2 × 108 TCID50/mL in Vero cells, and caused the formation of large syncytia. We analyzed the amino acid sequence of the HN-1 isolate and found that its S1 subunit contained a three-amino-acid insertion (355KRL358). A seven-amino-acid-deletion (1377FEKVHVQ1383) in the S2 subunit resulted in the partial deletion of the endocytosis signal YxxΦ and the complete deletion of the endoplasmic reticulum retrieval signal (ERRS) KVHVQ in the cytoplasmic tail of the S protein. Consequently, HN-1 is predicted to be less pathogenic than its parent strain, an attribute that facilitates rapid cell-to-cell spread by enhancing syncytium formation. In addition, strain HN-1 was found to have the mutation 884-885SG→RR, which may favor adaptation to cell culture by providing new trypsin cleavage sites. These results suggest that HN-1 is a G2c subtype variant that adapts well to cell culture and can be used to study the adaptive mechanisms of PEDV and develop attenuated vaccines.

First Report of Dalbulus maidis (DeLong and Wolcott) (Hemiptera: Cicadellidae) in Oklahoma.

The corn leafhopper, Dalbulus maidis (DeLong and Wolcott) (Hemiptera: Cicadellidae), is an invasive insect that can cause damage to maize (Zea mays L.) in two ways: by direct feeding and the transmission of several plant pathogens. Dalbulus maidis is an invasive and serious economic pest of maize that has spread from its center of origin in Mexico to the southernmost parts of the United States. Prior to 2024, corn leafhoppers had not been documented in Oklahoma, and their spread northward toward the United States corn belt is of significant concern. Here, we provide the first reports of the insect in maize in several Oklahoma counties. Insect specimens were collected at various commercial and experimental field sites by Oklahoma State University research and extension personnel. The identity of the insect species was validated through morphological and molecular taxonomy. The presence records for the corn leafhopper presented here provide valuable information for future monitoring and management efforts of this economically important pest and disease.

Eco-friendly Management of Alternaria Leaf Spot of Brinjal (Solanum melongena L.)

Among vegetables, brinjal or eggplant is an economically important vegetable crop and second major vegetable crop next to potato in India. This crop suffers from various diseases, among different fungal diseases of brinjal, Alternaria leaf spot caused by Alternaria alternata has currently become one of the most important diseases for all brinjal varieties. Loss due to the disease has been estimated up to 25-30 per cent. Therefore, present investigation was undertaken at Central Research Field of Sam Higginbottom University of Agriculture, Technology And Sciences, Prayagraj during the Kharif of 2023-2024, for the management of leaf spot using botanicals, bio-agent, cow urine and a fungicide (treated check) for their efficacy in vivo against Alternaria leaf spot of brinjal incited by Alternaria alternata. Effects of different botanicals, bio-agent and cow urine were evaluated on different growth, disease management and yield parameters of brinjal crop under field condition by foliar spray of botanicals, cow urine and a bio-agent, along with seedling treatment with Trichoderma viride at selected concentration. The effects of treatments on growth parameter were recorded i.e. maximum plant height at 30 DAT (22.78 cm), 60 DAT (37.16 cm), 90 DAT (53.87 cm) and maximum number of leaves at 30 DAT (10.83), 60 DAT (31.80), 90 DAT (47.33) and maximum number of branches at 30 DAT (4.23), 60 DAT (11.40), 90 DAT (13.83). Among the treatments, neem leaf extract + Trichoderma viride was recorded significantly reducing disease intensity at 45 DAT (10.32%), 60 DAT (16.11%), 75 DAT (27.93%) of brinjal followed by clove extract + Trichoderma viride and Trichoderma viride + T. viride as compared to mancozeb (treated check) and control. The maximum yield was found to be in neem leaf extract + Trichoderma viride i.e. (16.63 t/ha) followed by clove extract + Trichoderma viride, and Trichoderma viride + T. viride as compared to mancozeb (treated check) and control and the most economical treatment was Neem leaf extract + Trichoderma viride i.e. (1:2.62).

Neonatal tetanus in South Sudan: a case series

Introduction: Neonatal tetanus is a life-threatening disease of public health importance; it is yet to be eliminated and is still occurring in South Sudan. It is caused by a neurotoxin from a bacterium Clostridium tetani whose spores exist in the environment. Compared to high-income countries, most low-income countries lack intensive care units, and magnesium sulfate shown to improve neonatal tetanus outcomes. Objectives: We aimed to determine the outcome of neonatal tetanus in Al Sabah Children’s Hospital, Juba, South Sudan. Methods: We conducted a case series study in Al Sabah Children’s Hospital in Juba, South Sudan. It described three neonates who presented within the first seven days of life with a history of excessive crying, inability to breastfeed, and tetanic muscle spasms when stimulated. They had signs of respiratory distress, fever, and labile heart rates. We made a diagnosis of neonatal tetanus with autonomic dysfunction, and started them on phenobarbitone because diazepam was not available, oxygen, and antibiotics. However, tetanus immune globulin and anti-tetanus serum were not available. Results: Unfortunately, all three neonates died within 24 hours of admission. Conclusion: Our primary focus as a nation is to improve access to quality health services and the prevention of neonatal tetanus through encouraging appropriate antenatal care, facility delivery, clean delivery and healthy umblical cord care practices, and tetanus vaccinations in teenage girls and young adults. When prevention fails, there is a need for neonatal intensive care with recommended medicines to significantly decrease mortality in this preventable tragic illness. Keywords: Neonatal tetanus; low-income countries; neonates; Al sabah children hospital; South Sudan.

Mechanism of collagen type IV regulation by focal adhesion kinase during retained fetal membranes in dairy cows

Retained fetal membranes (RFM) is an important reproductive disease in dairy cows, caused by maternal and fetal placental tissue adhesion. The main collagen in maternal and fetal placenta tissues is collagen type IV (COL-IV) and its breakdown is the key to placental expulsion. Focal adhesion kinase (FAK) has been shown to regulate the hydrolysis of Col-IV by affecting the activity of MMP-2 and MMP-9 activity, but the regulation of the mechanisms involved in placenta expulsion in dairy cows after postpartum are still unclear. The aim of this study was to investigate the pathogenic mechanism of RFM by studying the relationship between the FAK signaling pathway and COL-IV regulation. Maternal placental tissues were collected from six healthy and six cows with RFM of similar age, parity, body condition and milk yield at 12 h postpartum. In vitro experiments were performed on bovine endometrial epithelial cells from three groups including a FAK inhibitor group, a FAK activator group and a control group without FAK inhibitor and activator. The abundance of molecules involved in the FAK signaling pathway and COL-IV was detected by immunohistochemistry, quantitative real-time polymerase chain reaction (qRT-PCR) and western blot. The immunohistochemical results showed that the key molecules of FAK signaling pathway FAK, Src, MMP-2 and MMP-9 and Col-IV were expressed in placental tissues. The expression level of FAK, Src, MMP-2, and MMP-9 were significantly down-regulated (P < 0.05) and the abundances of COL-IV were significantly up-regulated (P < 0.05) in maternal placental tissues of RFM cows compared with healthy cows. In the FAK inhibitor treatment group, the relative expression levels of FAK and other related proteins were significantly down-regulated (P < 0.05) and the relative expression levels of COL-IV were significantly up-regulated (P < 0.05) with the results of the FAK activation group the opposite. These results indicated that FAK in maternal endometrial epithelial cells could regulate the hydrolysis process of Col-IV through the expression of key factors of signaling pathways and promote collagen hydrolysis, which in turn facilitated the process of postpartum placenta expulsion in dairy cows.

Weather Based Forewarning Systems for Important Pests and Diseases in Major Crops

In present - day agriculture, weather based forewarning systems have become a vital tool in combating pests and diseases. These systems forecast the occurrence and severity of important insect pests and diseases using the meteorological data provided. The data regarding important meteorological variables like temperature, rainfall, humidity, wind speed, and wind patterns are integrated in these systems. As forewarning systems use highly developed artificial intelligence technology, historical data analysis and pest-weather correlations to provide precise and timely insights to the farmer community, which enable them to take appropriate control measures when required and help in reducing crop losses and optimising the pesticide usage. These will help to schedule pesticide applications as needed, ultimately reducing the quantity of pesticides sprayed and their detrimental impact on the environment and other beneficial organisms and increasing the overall agricultural output, which is very important in sustainable agriculture. To unlock the full potential of these systems, issues regarding data availability, localizing the systems to particular regions, and the effects of climate change should be addressed.

Predominance of Trichophyton soudanense as Agent of Dermatophytoses in Cape Verdean School-Age Children.

Dermatophytoses are infectious skin diseases of public health importance because of their transmissibility and high prevalence, especially among children. This is the first study aiming to estimate and report the burden of dermatophytoses on school-age children on the island of Santiago in Cape Verde, an African country that is an archipelago. A total of 249 students attending the afternoon shift of three elementary schools in the city of Achada Igreja were examined. Of these, 60 had suspected lesions of dermatophyte infection. However, from the samples collected from these 60 students, including hair, nails, and skin scrapings, only 18 dermatophyte isolates were obtained, corresponding to a point prevalence of 7.2%. Morphological species identification demonstrated three different species: Trichophyton soudanense, Trichophyton rubrum, and Trichophyton violaceum; re-identification by sequencing the internal transcribed spacer (ITS) 1 and 2 regions of ribosomal DNA, and the 5.8S rDNA encoding gene (ITS-5.8S region), revealed T. soudanense as the most prevalent species, with only one case of T. rubrum. This is the first epidemiological data describing dermatophytoses and dermatophytes in Cape Verde among school-age children on one of the archipelago islands. It reinforces the need of using culture and accurate identification methodologies when gathering epidemiological data on dermatophytoses.

Foliar blight, leaf spot, and stem blackening diseases caused by Stemphylium beticola, Alternaria alternata, and Alternaria atra on dragon's head (Lallemantia iberica) in Iran

Dragon's head, Lallemantia iberica, is an oilseed crop adapted to be grown in cold-dry areas. In the growing season of 2022–23 a severe foliar blight, leaf spot, and stem blacking symptoms were observed in commercial fields in northern and northwestern Iran, leading to more than 50 % yield loss. Infected samples were collected and transferred to the laboratory. Following the isolation of fungal agents, five representative isolates were selected for further studies. Morphological studies, based on conidial shape, conidiation, and other mycological characteristics, were carried out on a pure culture established from a single spore. Furthermore, multigene phylogenetic analyses were performed using sequences of translation elongation factor 1-α (TEF1), glyceraldehyde 3-phosphate dehydrogenase (GAPDH), RNA polymerase II second largest subunit (RPB2), and Internal Transcribed Spacer (ITS) rDNA genes for Alternaria isolates, along with calmodulin (cmdA) for the Stemphylium isolate. The morphological and molecular studies identified the fungal isolates as Alternaria atra, Alternaria alternata, and Stemphyliium beticola. Pathogenicity tests on the commercial cultivar ‘Sara’ indicated that all isolates were pathogenic to dragon's head, but they differed significantly in aggressiveness. To the best of our knowledge, this is the first report of the association of these fungal species with an important foliar disease on this crop in Iran and worldwide.