Forensic entomology employs the biology and ecology of insects in medico-legal cases. For a precise application in this context, it is necessary to make a correct taxonomic identification in any developmental stage of the specimens related to a case. Incomplete or damaged samples of adults are often obtained from the case scene, making taxonomical identification unlikely. Muscidae is one of the families commonly found in the forensic context. The objective of this study is to describe and to illustrate the morphological characteristics of the adult male genitalia of Biopyrellia bipuncta (Wiedemann, 1830), Musca domestica Linnaeus, 1758 and Synthesiomyia nudiseta (Wulp, 1883), species with forensic importance in Colombia. Male genitalia structures like V sternite, the cercal plate, the surstyli and aedeagus are described and illustrated. Additionally complementary Diagnosis of external morphological characteristics are provided.