Implicit Sequence Learning Research Articles

Exploring brain plasticity in developmental dyslexia through implicit sequence learning.

Developmental dyslexia (DD) is defined as difficulties in learning to read even with normal intelligence and adequate educational guidance. Deficits in implicit sequence learning (ISL) abilities have been reported in children with DD. We investigated brain plasticity in a group of 17 children with DD, compared with 18 typically developing (TD) children, after two sessions of training on a serial reaction time (SRT) task with a 24-h interval. Our outcome measures for the task were: a sequence-specific implicit learning measure (ISL), entailing implicit recognition and learning of sequential associations; and a general visuomotor skill learning measure (GSL). Gray matter volume (GMV) increased, and white matter volume (WMV) decreased from day 1 to day 2 in cerebellar areas regardless of group. A moderating effect of group was found on the correlation between WMV underlying the left precentral gyrus at day 2 and the change in ISL performance, suggesting the use of different underlying learning mechanisms in DD and TD children during the ISL task. Moreover, DD had larger WMV in the posterior thalamic radiation compared with TD, supporting previous reports of atypical development of this structure in DD. Further studies with larger sample sizes are warranted to validate these results.

Training-related changes in neural beta oscillations associated with implicit and explicit motor sequence learning

Many motor actions we perform have a sequential nature while learning a motor sequence involves both implicit and explicit processes. In this work, we developed a task design where participants concurrently learn an implicit and an explicit motor sequence across five training sessions, with EEG recordings at sessions 1 and 5. This intra-subject approach allowed us to study training-induced behavioral and neural changes specific to the explicit and implicit components. Based on previous reports of beta power modulations in sensorimotor networks related to sequence learning, we focused our analysis on beta oscillations at motor-cortical sites. On a behavioral level, substantial performance gains were evident early in learning in the explicit condition, plus slower performance gains across training sessions in both explicit and implicit sequence learning. Consistent with the behavioral trends, we observed a training-related increase in beta power in both sequence learning conditions, while the explicit condition displayed stronger beta power suppression during early learning. The initially stronger beta suppression and subsequent increase in beta power specific to the explicit component, correlated with enhanced behavioral performance, possibly reflecting higher cortical excitability. Our study suggests an involvement of motor-cortical beta oscillations in the explicit component of motor sequence learning.

Differential effects of bilateral hippocampal CA3 damage on the implicit learning and recognition of complex event sequences

ABSTRACT Learning regularities in the environment is a fundament of human cognition, which is supported by a network of brain regions that include the hippocampus. In two experiments, we assessed the effects of selective bilateral damage to human hippocampal subregion CA3, which was associated with autobiographical episodic amnesia extending ~50 years prior to the damage, on the ability to recognize complex, deterministic event sequences presented either in a spatial or a non-spatial configuration. In contrast to findings from related paradigms, modalities, and homologue species, hippocampal damage did not preclude recognition memory for an event sequence studied and tested at four spatial locations, whereas recognition memory for an event sequence presented at a single location was at chance. In two additional experiments, recognition memory for novel single-items was intact, whereas the ability to recognize novel single-items in a different location from that presented at study was at chance. The results are at variance with a general role of the hippocampus in the learning and recognition of complex event sequences based on non-adjacent spatial and temporal dependencies. We discuss the impact of the results on established theoretical accounts of the hippocampal contributions to implicit sequence learning and episodic memory.

Kinesthetic vs. visual focus: No evidence for effects of practice modality in representation types after action imagery practice and action execution practice

Action-imagery practice (AIP) is assumed to result in partly different action representations than action-execution practice (AEP). The present study investigated whether focusing on either kinesthetic or visual aspects of a task during practice amplifies or diminishes such differences between AIP and AEP. In ten sessions, four groups, using either AIP or AEP with either kinesthetic or visual focus, practiced a twelve-element sequence in a unimanual serial reaction time task. Tests involved the practice sequence, a mirror sequence, and a different sequence, each performed with the practice and transfer hand. In AIP and AEP, in both hands, reaction times (RTs) were shorter in the practice sequence than in the different sequence, indicating effector-independent visual-spatial sequence representations. Further, RTs were shorter in the practice hand than in the transfer hand in the practice sequence (but not in the different sequence), indicating effector-dependent representations in AEP and AIP. Although the representation types did not differ, learning effects were stronger in AEP than in AIP. Thus, although to a lower extent than in AEP, effector-dependent representations can be acquired using AIP. Contrary to the expectations, the focus manipulation did not have an impact on the acquired representation types. Hence, modality instructions in AIP may not have such a strong impact as commonly assumed, at least in implicit sequence learning.

Resting state electroencephalography power correlates with individual differences in implicit sequence learning.

Neuroimaging resting state paradigms have revealed synchronised oscillatory activity is present even in the absence of completing a task or mental operation. One function of this neural activity is likely to optimise the brain's sensitivity to forthcoming information that, in turn, likely promotes subsequent learning and memory outcomes. The current study investigated whether this extends to implicit forms of learning. A total of 85 healthy adults participated in the study. Resting state electroencephalography was first acquired from participants before they completed a serial reaction time task. On this task, participants implicitly learnt a visuospatial-motor sequence. Permutation testing revealed a negative correlation between implicit sequence learning and resting state power in the upper theta band (6-7Hz). That is, lower levels of resting state power in this frequency range were associated with superior levels of implicit sequence learning. This association was observed at midline-frontal, right-frontal and left-posterior electrodes. Oscillatory activity in the upper theta band supports a range of top-down processes including attention, inhibitory control and working memory, perhaps just for visuospatial information. Our results may be indicating that disengaging theta-supported top-down attentional processes improves implicit learning of visuospatial-motor information that is embedded in sensory input. This may occur because the brain's sensitivity to this type of information is optimally achieved when learning is driven by bottom-up processes. Moreover, the results of this study further demonstrate that resting state synchronised brain activity influences subsequent learning and memory.

Concurrent visual sequence learning

Many researchers in the field of implicit statistical learning agree that there does not exist one general implicit learning mechanism, but rather, that implicit learning takes place in highly specialized encapsulated modules. However, the exact representational content of these modules is still under debate. While there is ample evidence for a distinction between modalities (e.g., visual, auditory perception), the representational content of the modules might even be distinguished by features within the same modalities (e.g., location, color, and shape within the visual modality). In implicit sequence learning, there is evidence for the latter hypothesis, as a stimulus-color sequence can be learned concurrently with a stimulus-location sequence. Our aim was to test whether this also holds true for non-spatial features within the visual modality. This has been shown in artificial grammar learning, but not yet in implicit sequence learning. Hence, in Experiment 1, we replicated an artificial grammar learning experiment of Conway and Christiansen (2006) in which participants were supposed to learn color and shape grammars concurrently. In Experiment 2, we investigated concurrent learning of sequences with an implicit sequence learning paradigm: the serial reaction time task. Here, we found evidence for concurrent learning of two sequences, a color and shape sequence. Overall, the findings converge to the assumption that implicit learning might be based on features.

The effect of response-to-stimulus interval on children’s implicit sequence learning

The current study examined, for the first time in a developmental perspective, the effect of response-to-stimulus interval (RSI) in incidental sequence learning (SL). Children aged 4, 7, and 10 years performed a serial reaction time (SRT) task in which the RSI was systematically manipulated (0, 250, 500, or 750 ms). SL (difference in reaction times between fixed and random blocks) was not observed for the youngest children whatever the RSI condition, whereas the 7-year-olds learned the sequence only in the 250-ms RSI condition and the 10-year-olds exhibited SL in all temporal conditions except the 500-ms RSI condition. Finally, the results suggest that conscious awareness of the sequence emerges only in older children faced with the 500- and 750-ms RSI conditions. The discussion questions the robustness of implicit learning processes in the light of individual and contextual factors.

Imagine to automatize: automatization of stimulus-response coupling after action imagery practice in implicit sequence learning.

Action imagery practice (AIP) describes the repetitive imagination of an action to improve subsequent action execution. Because AIP and action execution practice (AEP) draw on partly similar motor mechanisms, it was assumed that AIP may lead to motor automatization, which is observable in a reduction of dual-task costs after AEP. To investigate automatization in AIP, we compared dual-task and single-task performance in practice and random sequences in pretests and posttests. All participants practiced serial reactions to visual stimuli in ten single-task practice sessions. An AIP group imagined the reactions. An AEP group and a control practice group executed the reactions. Practice followed a sequential sequence in AIP and AEP but was random in control practice. In dual-task test conditions, tones were counted that appeared in addition to the visual stimuli. RTs decreased from pretest to posttest in both practice and random sequences in all groups indicating general sequence-unspecific learning. Further, RTs decreased to a greater extent in the practice sequence than in the random sequence after AIP and AEP, indicating sequence-specific learning. Dual-task costs-the difference between RTs after tone and no tone events-were reduced independent from the performed sequence in all groups indicating sequence-unspecific automatization. It is concluded that the stimulus-response coupling can be automatized by both, AEP and AIP.

Time course of learning sequence representations in action imagery practice

Action imagery practice (AIP) is effective to improve motor performance in a variety of tasks, though it is often less effective than action execution practice (AEP). In sequence learning, AIP and AEP result in the acquisition of effector-independent representations. However, it is unresolved whether effector-dependent representations can be acquired in AIP. In the present study, we investigated the acquisition of effector-independent representations and effector-dependent representations in AEP and AIP in an implicit sequence learning task (a visual serial-reaction-time task, involving a twelve-element sequence). Participants performed six sessions, each starting with tests. A practice sequence, a mirror sequence, and a different sequence were tested with the practice and transfer hand. In the first four sessions, after the tests, two groups performed either AIP (N = 50) or AEP (N = 54). Improvement in the different sequence indicated sequence-unspecific learning in both AEP and AIP. Importantly, reaction times of the practice hand became shorter in the practice sequence than in the other sequences, indicating implicit sequence learning in both, AEP and AIP. This effect was stronger in the practice hand than in the transfer hand, indicating effector-dependent sequence representations in both AEP and AIP. However, effector-dependent sequence representations were stronger in AEP than in AIP. No significant differences between groups were observed in the transfer hand, although effector-independent sequence representations were observed in AEP only. In conclusion, AIP promotes not only sequence-unspecific stimulus-response coupling and anticipations of the subsequent stimuli, but also anticipations of the subsequent responses.

Exploring an online method of measuring implicit sequence-learning consciousness.

Existing methods for measuring implicit sequence-learning consciousness are conducted offline. Based on the traditional measurement of cued-generation task, this study implemented an online measurement method by converting a generation task into a forced-choice task to observe the dynamic changes of consciousness in the implicit sequence-learning process. In this study, we compared the performance of online measurement task and traditional sequence-learning tasks in 31 university students. The results revealed that the online indicators were significantly correlated with classic consciousness indicators and typical ERP components of consciousness. Without affecting the development of consciousness, the online measurement indicators were found to promptly and effectively reflect the gradually changing progression of consciousness in implicit sequence learning.

Long-term memory predictors of adult language learning at the interface between syntactic form and meaning.

Recent neurocognitive models of second language learning have posited specific roles for declarative and procedural memory in the processing of novel linguistic stimuli. Pursuing this line of investigation, the present exploratory study examined the role of declarative and procedural memory abilities in the early stages of adult comprehension of sentences in a miniature language with natural language characteristics (BrocantoJ). Thirty-six native Italian young adults were aurally exposed to BrocantoJ in the context of a computer game over three sessions on consecutive days. Following vocabulary training and passive exposure, participants were asked to perform game moves described by aural sentences in the language. Game trials differed with respect to the information the visual context offered. In part of the trials processing of relationships between grammatical properties of the language (word order and morphological case marking) and noun semantics (thematic role) was necessary in order reach an accurate outcome, whereas in others nongrammatical contextual cues were sufficient. Declarative and procedural learning abilities were respectively indexed by visual and verbal declarative memory measures and by a measure of visual implicit sequence learning. Overall, the results indicated a substantial role of declarative learning ability in the early stages of sentence comprehension, thus confirming theoretical predictions and the findings of previous similar studies in miniature artificial language paradigms. However, for trials that specifically probed the learning of relationships between morphosyntax and semantics, a positive interaction between declarative and procedural learning ability also emerged, indicating the cooperative engagement of both types of learning abilities in the processing of relationships between ruled-based grammar and interpretation in the early stages of exposure to a new language in adults.

Response Time Modulates the Relationship Between Implicit Learning and Motor Ability in Children With and Without Autism Spectrum Disorders: A Preliminary Study.

Difficulty with implicit learning plays an important role in the symptomology of autism spectrum disorder (ASD). However, findings in motor learning are inconsistent. This study evaluated implicit sequence learning and its relationship with motor ability in children with and without ASD. We adopted a classic serial reaction time task with a retention task and three awareness tests. The Movement Assessment Battery for Children was administered to assess children's motor ability. Significant learning differences between children with and without ASD were only found in retention but not immediately after the serial reaction time task. These findings suggest that the impaired implicit learning in ASD is characterized as impaired consolidation where the relatively permanent changes are missing. Exploratory moderation analyses revealed a significant relationship between implicit learning and motor ability for individuals with faster response time. We argue the importance of response speed for optimal learning and should be weighted more for future intervention in children with ASD.

Neural basis of implicit motor sequence learning: Modulation of cortical power.

Implicit sequence learning describes the acquisition of serially ordered movements and sequentially structured cognitive information, that occurs without awareness. Theta, alpha and beta cortical oscillations are present during implicit motor sequence learning, but their role in this process is unclear. The current study addressed this gap in the literature. A total of 50 healthy adults aged between 19 and 37 years participated in the study. Implicit motor sequence learning was examined using the Serial Reaction Time task where participants unknowingly repeat a sequence of finger movements in response to a visual stimulus. Sequence learning was examined by comparing reaction times and oscillatory power between sequence trials and a set of control trials comprising random stimulus presentations. Electroencephalography was recorded as participants completed the task. Analyses of the behavioral data revealed participants learnt the sequence. Analyses of oscillatory activity, using permutation testing, revealed sequence learning was associated with a decrease in theta band (4-7Hz) power recorded over frontal and central electrode sites. Sequence learning effects were not observed in the alpha (7-12 Hz) or beta bands (12-20 Hz). Even though alpha and beta power modulations have long been associated with executing a motor response, it seems theta power is a correlate of sequence learning in the manual domain. Theta power modulations on the serial reaction time task may reflect disengagement of attentional resources, either promoting or occurring as a consequence of implicit motor sequence learning.

Cognitive control modulates the expression of implicit sequence learning: Congruency sequence and oddball-dependent sequence effects.

Implicit sequence learning represents an established paradigm to investigate incidental skill acquisition in a laboratory environment. During a covert task, participants respond to the location of a target appearing over a series of locations according to a complex sequence, which gets violated in a reduced set of control trials. Even though participants are not fully aware of the sequence, they respond faster and more accurately to trials following it, thus expressing sequence knowledge. Recent evidence has challenged the view that such knowledge is applied rigidly and affects performance independently from control influences. Jiménez et al. (2009) highlighted that its expression gets reduced immediately after trials not conforming with the learned sequence-an effect that resembles the congruency sequence effect (CSE) commonly observed in interference tasks. However, such effects can also be alternatively explained in associative terms. In this experimental series we took advantage of the well-known attentional properties of oddball sounds and introduced them as an orthogonal variable with respect to the learning process. We found that oddball sounds also hindered the automatic expression of sequence learning, highlighting an oddball-dependent sequence effect similar to the CSE, but most clearly triggered by cognitive control. Moreover, as illustrated through a simulation with a simple recurrent network (SRN), we showed that the CSE reported in this article under noisier conditions is harder to expect from associative processes. (PsycInfo Database Record (c) 2022 APA, all rights reserved).

Exploring the representational mechanism of implicit sequence learning: Evidence from eye movements

Exploring the representational mechanism of implicit sequence learning: Evidence from eye movements

Effects of visuospatial implicit sequence learning on visual stimulus processing: Evidence from event-related potentials and neural synchrony

A previous study reported that reaction times (RTs) and the amplitude of the P1 component of event-related potentials (ERPs) elicited by visual stimuli decreased during visuospatial implicit sequence learning in the serial reaction time task, suggesting that sequence learning reduces attentional demands on visual stimulus processing. In the present study, to evaluate the replicability of the previous finding and to obtain a better understanding of how visual stimulus processing is affected by visuospatial implicit sequence learning, we measured ERPs and neural synchrony from 44 participants during a modified serial reaction time task which controlled for a possible confounding factor in the previous study (i.e., arousal). The results indicated that RTs and neural synchrony of the lower frequency band (22–34 Hz) decreased for a learned sequence, whereas no significant effects on the amplitudes of P1, N1, and P3 components of ERPs were observed. These results suggest that attentional demands on visual stimulus processing can be reduced by visuospatial implicit sequence learning, as suggested by the previous study, but stimulus-locked ERPs may not be sensitive enough to reflect such learning effects.

Muscle activity in explicit and implicit sequence learning: Exploring additional measures of learning and certainty via tensor decomposition

Sequence learning in serial reaction time tasks (SRTTs) is usually inferred through the reaction time measured by a keyboard. However, this chronometric parameter offers no information beyond the time point of the button-press. We therefore examined whether sequence learning can be measured by muscle activations via electromyography (EMG) in a dual-task paradigm. The primary task was a SRTT, in which the stimuli followed a fixed sequence in some blocks, whereas the sequence was random in the control condition. The secondary task stimulus was always random. One group was informed about the fixed sequence, and the other not. We assessed three dependent variables. The chronometric parameter premotor time represents the duration between stimulus onset and the onset of EMG activity, which indicates the start of the response. The other variables describe the response itself considering the EMG activity after response start. The EMG integral was analyzed, and additionally, tensor decomposition was implemented to assess sequence dependent changes in the contribution of the obtained subcomponents. The results show explicit sequence learning in this dual-task setting. Specifically, the informed group show shorter premotor times in fixed than random sequences as well as larger EMG integral and tensor contributions. Further, increased activity seems to represent response certainty, since a decrease is found for both groups in trials following erroneous responses. Interestingly, the sensitivity to sequence and post-error effects varies between the subcomponents. The results indicate that muscle activity can be a useful indicator of response behavior in addition to chronometric parameters.

Maybe causal, but still cautious: Reply to “Cautious or causal? Key implicit sequence learning paradigms should not be overlooked when assessing the role of DLPFC (Commentary on Prutean et al.)”

Maybe causal, but still cautious: Reply to “Cautious or causal? Key implicit sequence learning paradigms should not be overlooked when assessing the role of DLPFC (Commentary on Prutean et al.)”

Supplemental Material for Cognitive Control Modulates the Expression of Implicit Sequence Learning: Congruency Sequence and Oddball-Dependent Sequence Effects

Supplemental Material for Cognitive Control Modulates the Expression of Implicit Sequence Learning: Congruency Sequence and Oddball-Dependent Sequence Effects

The interplay between unexpected events and behavior in the development of explicit knowledge in implicit sequence learning

Some studies in implicit learning investigate the mechanisms by which implicitly acquired knowledge (e.g., learning a sequence of responses) becomes consciously aware. It has been suggested that unexpected changes in the own behavior can trigger search processes, of which the outcome then becomes aware. A consistent empirical finding is that participants who develop explicit knowledge show a sudden decrease in reaction times, when responding to sequential events. This so called RT-drop might indicate the point of time when explicit knowledge occurs. We investigated whether an RT-drop is a precursor for the development of explicit knowledge or the consequence of explicit knowledge. To answer this question, we manipulated in a serial reaction time task the timing of long and short stimulus-onset asynchronies (SOA). For some participants, the different SOAs were presented in blocks of either long or short SOAs, while for others, the SOAs changed randomly. We expected the participants who were given a blocked presentation to express an RT-drop because of the predictable timing. In contrast, randomly changing SOAs should hamper the expression of an RT-drop. We found that more participants in the blocked-SOA condition than in the random-SOA condition showed an RT-drop. Furthermore, the amount of explicit knowledge did not differ between the two conditions. The findings suggest that the RT-drop does not seem to be a presupposition to develop explicit knowledge. Rather, it seems that the RT-drop indicates a behavioral strategy shift as a consequence of explicit knowledge.