Implications For Human Health Research Articles

Public perceptions of the health impacts of climate change: a survey study in Greece

Abstract Background Climate change is the biggest global health threat of the 21st century. Despite the tremendous challenges, many people remain unaware of the human health implications of climate change. People perceptions about the health harms of climate change can increase public support for the action needed to reduce climate change. The aim of this study was to examine the public perception of climate change as a human health issue in Greece. Methods A population survey study was conducted from October to December 2023 in the municipalities of Messini and Alexandroupoli, Greece, following proportional quota sampling. A questionnaire was developed and included measures about the socio-demographic background of participants, their understanding of key facts about climate change and their views about climate change thread to human health. Results 406 individuals with a mean age 37.7 (sd = 14.8) participated in the study. Participants understood that climate change is happening (93.6%) and 54% of them think climate change is mostly or entirely caused by human activities, whereas 38% think it is caused equally by human activities and natural causes, and about 8% think climate change is mostly or entirely caused by natural causes. A majority of participants reported that climate change has adversely affected the health of people in various way, either a moderate amount or a great deal, including physical or mental harm from forest fires (90%), physical or mental harm from storms and floods (87%), heat-related illnesses (82%), illness due to reduced outdoor air quality (70%) and loss of housing for residents displaced by extreme weather events (70%). Conclusions Participants viewed climate change as an important cause of health harm, however they partially understood that climate change is caused by humans. Public health authorities should educate the public and policymakers about the climate change and advocate for policies to address the human health challenges of climate change. Key messages • Public health authorities should educate the public and policymakers about the climate change problem and its impact to human health. • Providing people with information about the health harms of climate change can increase public support for the actions needed to reduce emissions.

Host–microbe interaction-mediated resistance to DSS-induced inflammatory enteritis in sheep

BackgroundThe disease resistance phenotype is closely related to immunomodulatory function and immune tolerance and has far-reaching implications in animal husbandry and human health. Microbes play an important role in the initiation, prevention, and treatment of diseases, but the mechanisms of host–microbiota interactions in disease-resistant phenotypes are poorly understood. In this study, we hope to uncover and explain the role of microbes in intestinal diseases and their mechanisms of action to identify new potential treatments.MethodsFirst, we established the colitis model of DSS in two breeds of sheep and then collected the samples for multi-omics testing including metagenes, metabolome, and transcriptome. Next, we made the fecal bacteria liquid from the four groups of sheep feces collected from H-CON, H-DSS, E-CON, and E-DSS to transplant the fecal bacteria into mice. H-CON feces were transplanted into mice named HH group and H-DSS feces were transplanted into mice named HD group and Roseburia bacteria treatment named HDR groups. E-CON feces were transplanted into mice named EH group and E-DSS feces were transplanted into mice in the ED group and Roseburia bacteria treatment named EDR groups. After successful modeling, samples were taken for multi-omics testing. Finally, colitis mice in HD group and ED group were administrated with Roseburia bacteria, and the treatment effect was evaluated by H&E, PAS, immunohistochemistry, and other experimental methods.ResultsThe difference in disease resistance of sheep to DSS-induced colitis disease is mainly due to the increase in the abundance of Roseburia bacteria and the increase of bile acid secretion in the intestinal tract of Hu sheep in addition to the accumulation of potentially harmful bacteria in the intestine when the disease occurs, which makes the disease resistance of Hu sheep stronger under the same disease conditions. However, the enrichment of harmful microorganisms in East Friesian sheep activated the TNFα signalling pathway, which aggravated the intestinal injury, and then the treatment of FMT mice by culturing Roseburia bacteria found that Roseburia bacteria had a good curative effect on colitis.ConclusionOur study showed that in H-DSS-treated sheep, the intestinal barrier is stabilized with an increase in the abundance of beneficial microorganisms. Our data also suggest that Roseburia bacteria have a protective effect on the intestinal barrier of Hu sheep. Accumulating evidence suggests that host–microbiota interactions are associated with IBD disease progression.2GpPdtDdh9Lk52sebv8JARVideo

Effect of Electrode Positioning on Electrokinetic Remediation of Contaminated Soft Clay with Surface Electrolyte.

Soft clay contamination is an increasingly global issue with significant implications for land development and human health. Electrokinetic remediation (EKR) has demonstrated significant potential for cleaning contaminated soils. It is crucial to develop efficient processes that minimize environmental impact and reduce costs. A series of citric acid (CA)-enhanced EKR tests were conducted using a novel experimental setup, with the electrolyte positioned above the soil surface, to examine the impact of four different electrode arrangements on the effectiveness of EKR. The position of the electrode end had a significant impact on the migration of ions in the anolyte and catholyte, which in turn affected the volume reduction in the anolyte, the magnitude of the current, and the migration of heavy metals. The electrode arrangement mode c (electrodes suspended in the electrolytes) can enhance the migration of the anolyte and reduce the drainage of the soil, making it an effective measure for improving the removal rate of heavy metals. After the heavy metal remediation is complete, the bearing capacity of the soil should be increased. Changing the electrode arrangement to mode d (anode suspended in the anolyte, a very small part of the cathode inserted into the soil) is an effective measure for reducing the soil water content and improving soil strength.

Spatial and Bioaccumulation of Heavy Metals in a Sheep-Based Food System: Implications for Human Health.

Heavy metal contamination in agricultural soils presents serious environmental and health risks. This study assessed the bioaccumulation and spatial distribution of nickel, cadmium, zinc, lead, and copper within a sheep-based food chain in the Baia Mare region, Romania, which includes soil, green grass, sheep serum, and dairy products. Using inductively coupled plasma mass spectrometry (ICP-MS), we analyzed the concentrations of these metals and calculated bioconcentration factors (BCFs) to evaluate their transfer through trophic levels. Spatial analysis revealed that copper (up to 2528.20 mg/kg) and zinc (up to 1821.40 mg/kg) exceeded permissible limits, particularly near former mining sites. Elevated lead (807.59 mg/kg) and cadmium (2.94 mg/kg) were observed in industrial areas, while nickel and cobalt showed lower concentrations, but with localized peaks. Zinc was the most abundant metal in grass, while cadmium transferred efficiently to milk and cheese, raising potential health concerns. The results underscore the complex interplay between soil properties, contamination sources, and biological processes in heavy metal accumulation. These findings highlight the importance of continuous monitoring, risk assessment, and mitigation strategies to protect public health from potential exposure through contaminated dairy products.

Chlorpyrifos and dimethoate exposure impairs female fertility by deregulating WNT signaling pathway & uterine receptivity

The study assessed histological, biochemical, oxidative stress, and molecular parameters to evaluate the consequences of Chlorpyrifos and Dimethoate exposure on uterine health in female rats. Despite showing no obvious signs of toxicity apart from minor clinical symptoms in DM-exposed rats, both pesticides caused degenerative changes in uterine tissue. This study demonstrates that pesticides induce inflammatory responses and oxidative stress in rats, by NF-κB activation and altering antioxidant enzyme levels. Besides, CPF and DM exposure disrupted gene expression of HOXA10, HOXA11, and WNT and reduced activation of β-catenin in the uterus, which is crucial for implantation and reproductive function. These findings suggest that pesticide exposure may impair reproductive health and fertility in females, highlighting potential implications for human health.

Assessment of Heavy Metal Contamination in Combretum micranthum Leaves and Decoctions: Implications for Human Health

Combretum micranthum is a well-known medicinal plant in Africa and the decoction of its leaves is used as a drink or to treat various diseases such as malaria, high blood pressure and liver disorders, among others. Due to its high consumption by all sections of the Senegalese population, we felt that its heavy metal intake should be examined to see its impact on health. Leaves of two species of Combretum micranthum produced in the central (I) and southern (II) regions of Senegal and their decoctions in ultrapure water (Ii) and (IIi) were analyzed for 12 elements (Cr, Mn, Fe, Co, Ni, Cu, Zn, As, Ag, Cd, Pb, Bi) by inductively coupled plasma optical emission spectrometer (ICP-OES). The concentrations of heavy metals (μg/g) are in the ranges 2.55 – 84.19 for Cr, 5.21 – 379.72 for Mn, 0.00 – 317.80 for Fe, 0.08 – 1.08 for Co, 1.54 – 8.88 for Ni, 1.75 – 8.08 for Cu, 0.91 – 9.70 for Zn, 0.00 – 0.16 for Cd, 0.46 – 0.84 for Pb. As, Ag and Bi are below detectable limits in any of the samples by ICP-OES analysis. Among all samples, manganese content was highest and represented 46.85% for I , 64.18% for II , 86.88% for Ii and 46.27% for IIi , of the total heavy metal concentrations. The results showed that the leaves of both varieties of Combretum micranthum and decoctions made from these leaves contain heavy metal levels lower than the values recommended by the WHO for consumed medicinal plants. Overall, Variety I harvested from peri-urban areas contains more metals than Variety II harvested from forest.

Association Between Dairy Production System and Milk Functionality Based on Analysis of miRNAs in Exosomes from Milk.

Dairy farming practices significantly affect the nutritional and functional properties of milk. This study compares miRNAs in milk exosomes from extensive and intensive dairy systems and explores their potential implications for human consumers. Extensive systems are believed to produce milk of higher quality with better animal welfare compared to intensive systems. Milk samples from eight extensive and nine intensive dairy farms were analysed. Milk-derived extracellular vesicles were isolated through sequential ultracentrifugation and characterised through Dynamic Light Scattering and Nanosight to determine the size and the concentration of the extracellular vesicles, in addition to immunoblotting to ensure the presence of exosome-specific proteins in their membrane. miRNA levels were quantified using RT-qPCR, and metabolic pathways associated with miRNAs showing significant differences between farm groups were analysed. EVs from extensive farms had higher concentrations. Notably, bta-miR-451 levels were significantly higher in milk from extensive farms (p = 0.021). Like human miRNA hsa-miR-451, it is linked to pathways related to Parkinson's disease and cancer. Our research suggests that milk production in extensive systems not only provides socioeconomic and environmental benefits but may also have positive effects on human health. Further research is warranted to explore the bioactive potential of these miRNAs and their implications for human health.

Magnesium Matters: A Comprehensive Review of Its Vital Role in Health and Diseases.

Magnesium (Mg), an essential mineral abundantly present within the human body, is intricately involved in a multitude of biochemical processes vital for maintaining health and overall well-being. This review aims to delve into the multifaceted impact of Mg on human health, exploring its physiological functions, dietary sources, and potential health implications of deficiency or insufficiency. Mg plays a pivotal role in various physiological processes, including energy metabolism, muscle contraction, protein synthesis, and DNA synthesis. It acts as a cofactor for more than 300 enzymatic reactions, facilitating the conversion of adenosine triphosphate (ATP) to adenosine diphosphate (ADP) for energy production. Moreover, Mg is essential for the proper functioning of ion channels, particularly calcium channels, influencing nerve transmission and muscle relaxation. Mg is naturally found in a wide array of foods, with green leafy vegetables, whole grains, nuts, seeds, and legumes being particularly rich sources. Additionally, certain fortified foods and dietary supplements provide supplemental Mg intake. Deficiency or insufficiency of mg can have profound implications for health. Inadequate mg levels have been associated with increased risks of various chronic diseases, including hypertension, type 2 diabetes, osteoporosis, and cardiovascular diseases. Furthermore, mg deficiency may manifest as symptoms such as muscle weakness, fatigue, tremors, and irregular heartbeat. Numerous studies have elucidated the relationship between mg intake and the risk of developing chronic diseases. For instance, epidemiological evidence suggests that higher mg intake is associated with a reduced risk of hypertension, possibly due to its vasodilatory effects and influence on blood pressure regulation mechanisms. Similarly, mg has been implicated in the pathophysiology of type 2 diabetes, with mg deficiency contributing to insulin resistance and impaired glucose metabolism. Furthermore, adequate mg intake is crucial for maintaining bone density and reducing the risk of osteoporosis, as mg plays a vital role in bone mineralization and bone health. Understanding the importance of mg in human physiology underscores the significance of ensuring adequate mg intake through diet or supplementation. Healthcare professionals play a critical role in educating individuals about the importance of incorporating mg-rich foods into their diets and considering mg supplementation when necessary, particularly for individuals at risk of deficiency or those with chronic diseases. Mg is an indispensable mineral with far-reaching implications for human health. Its involvement in various physiological processes underscores its importance in maintaining overall health and well-being. Ensuring adequate mg intake is essential for preventing deficiency-related health complications and reducing the risk of chronic diseases. Further research is warranted to elucidate the optimal strategies for mg supplementation and its potential therapeutic applications in disease prevention and management.

Assessing Heavy Metal Contamination in Commonly Used Fertilizers for Polyculture Fish Ponds and Its Implications for Human Health: A Comprehensive Investigation.

Over-fertilizing fish ponds can cause pollution, introducing heavy metals into the food chain and posing health risks. The present study investigated the incidence of heavy metals (Pb, Cu, Cd, and Cr) in commonly applied fertilizers, including nitrogen, phosphorus and potassium (NPK), triple superphosphate (TSP), and di-ammonium phosphate (DAP), and their association with heavy metals in water, sediment, and cultured fish species (Catla catla, Labeo rohita, and Cyprinus carpio) in polyculture fish ponds. The study was conducted over 4months, with four groups in triplicates: control (no fertilizer), group 1 (NPK), group 2 (TSP), and group 3 (DAP). Heavy metal analysis was carried out using atomic absorption spectrophotometry before and after fertilizer application. Significantly (p < 0.05) higher levels of heavy metals were observed in water and sediment after applying fertilizers, with the most pronounced results in group 3 (DAP) followed by group 2 (TSP). The concentration of heavy metals was significantly (p < 0.05) higher in group 3 (DAP) fertilizers compared to other groups. Compared to the control, the concentration and bioaccumulation of heavy metals were significantly (p < 0.05) higher in the fertilizer-applied groups, with notably higher levels in group 3 (DAP). Cluster analysis and the correlation matrix did not show any significant association between the heavy metals and the fertilizers, indicating a complex interplay between the biotic and abiotic factors of the system. The health index (HI) value was < 1 in fish muscles of all studied groups, indicating the fish are safe for consumption. The study recommends monitoring and regulating fertilizer use, especially DAP, to prevent heavy metal contamination, and exploring sustainable alternatives to minimize environmental and health risks.

Extensive longevity and DNA virus-driven adaptation in nearctic Myotis bats.

The rich species diversity of bats encompasses extraordinary adaptations, including extreme longevity and tolerance to infectious disease. While traditional approaches using genetic screens in model organisms have uncovered some fundamental processes underlying these traits, model organisms do not possess the variation required to understand the evolution of traits with complex genetic architectures. In contrast, the advent of genomics at tree-of-life scales enables us to study the genetic interactions underlying these processes by leveraging millions of years of evolutionary trial-and-error. Here, we use the rich species diversity of the genus Myotis - one of the longest-living clades of mammals - to study the evolution of longevity-associated traits and infectious disease using functional evolutionary genomics. We generated reference genome assemblies and cell lines for 8 closely-related (∼11 MYA) species of Myotis rich in phenotypic and life history diversity. Using genome-wide screens of positive selection, analysis of structural variation and copy number variation, and functional experiments in primary cell lines, we identify new patterns of adaptation in longevity, cancer resistance, and viral interactions both within Myotis and across bats. We find that the rapid evolution of lifespan in Myotis has some of the most significant variations in cancer risk across mammals, and demonstrate a unique DNA damage response in the long-lived M. lucifugus using primary cell culture models. Furthermore, we find evidence of abundant adaptation in response to DNA viruses, but not RNA viruses, in Myotis and other bats. This is in contrast to these patterns of adaptation in humans, which might contribute to the importance of bats as a reservoir of zoonotic viruses. Together, our results demonstrate the utility of leveraging natural variation to understand the genomics of traits with implications for human health and suggest important pleiotropic relationships between infectious disease tolerance and cancer resistance.

Using ELISA to detect pathogen antibodies in wild mammal carcasses: a systematic literature review

Abstract Carcasses of wild mammals provide convenient samples for wildlife disease investigators. Specifically, antibodies can persist in the blood and tissues of animals up to a few days after death and can provide information on exposure to specific pathogens, including microorganisms with zoonotic implications, such as Toxoplasma gondii, Trichinella spp., Brucella spp., and Mycobacterium spp. Although screening samples from decaying wildlife for antibodies is not a new method, an overview of the literature with clear recommendations on best practices is lacking. Here, we present results from a systematic review of 115 studies using enzyme‐linked immunosorbent assay (ELISA) to detect pathogen antibodies in wild mammals across 298 individual research projects. We discuss the range of mammalian hosts and the pathogens most represented in the literature and suggest improvements to consider when using samples from decaying animals for seroinvestigation. Our results indicate that the most examined mammals were artiodactyl ungulates and that antibodies against the bacteria Brucella spp. are those most examined to date, followed by the protozoan Toxoplasma gondii, malignant catarrhal fever virus, Schmallenberg orthobunyavirus, and the coccidian Neospora caninum. The effect of haemolysis in serum samples on ELISA results was considered in only 3.7% of the research projects we reviewed, sample size decisions were not outlined in 83% of research projects, and half of the projects did not examine seroprevalence in different age classes. Nevertheless, these details are important for replicability and accurate interpretation of results. We highlight the benefits and limitations of detecting antibodies against pathogens in carcasses from wild mammalian groups. Wild mammals are reservoirs for a wide range of pathogens including those with zoonotic potential and investigating their carcasses can help wildlife investigators to better understand the epidemiology of many diseases with implications for wildlife, livestock, and human health.

Interactions of Polychlorinated Biphenyls and Their Metabolites with the Brain and Liver Transcriptome of Female Mice.

Exposure to polychlorinated biphenyls (PCBs) is linked to neurotoxic effects. This study aims to close knowledge gaps regarding the specific modes of action of PCBs in female C57BL/6J mice (>6 weeks) orally exposed for 7 weeks to a human-relevant PCB mixture (MARBLES mix) at 0, 0.1, 1, and 6 mg/kg body weight/day. PCB and hydroxylated PCB (OH-PCBs) levels were quantified in the brain, liver, and serum; RNA sequencing was performed in the striatum, prefrontal cortex, and liver, and metabolomic analyses were performed in the striatum. Profiles of PCBs but not their hydroxylated metabolites were similar in all tissues. In the prefrontal cortex, PCB exposure activated the oxidative phosphorylation respiration pathways, while suppressing the axon guidance pathway. PCB exposure significantly changed the expression of genes associated with neurodevelopmental and neurodegenerative diseases in the striatum, impacting pathways like growth hormone synthesis and dendrite development. PCBs did not affect the striatal metabolome. In contrast to the liver, which showed activation of metabolic processes following PCB exposure and the induction of cytochrome P450 enzymes, the expression of xenobiotic processing genes was not altered by PCB exposure in either brain region. Network analysis revealed complex interactions between individual PCBs (e.g., PCB28 [2,4,4'-trichlorobiphenyl]) and their hydroxylated metabolites and specific differentially expressed genes (DEGs), underscoring the need to characterize the association between specific PCBs and DEGs. These findings enhance the understanding of PCB neurotoxic mechanisms and their potential implications for human health.

The distinct roles of NEIL1 and XPA in limiting aflatoxin B₁-induced mutagenesis in mice.

Dietary exposure to aflatoxin B₁ (AFB₁) is a risk factor for the development of hepatocellular carcinomas (HCCs). Following metabolic activation, AFB₁ reacts with guanines to form covalent DNA adducts, which induce high-frequency G > T transversions. The molecular signature associated with these mutational events aligns with the single base substitution signature 24 (SBS24) in the Catalog of Somatic Mutations in Cancer (COSMIC) database. Deficiencies in either base excision repair (BER) due to the absence of Nei-like DNA glycosylase 1 (NEIL1) or nucleotide excision repair (NER) due to the absence of xeroderma complementation group A protein (XPA) contribute to HCCs in murine models. In the current study, ultra-low error duplex sequencing was used to characterize mutational profiles in liver DNAs of NEIL1-deficient, XPA-deficient, and DNA repair-proficient mice following neonatal injection of 1 mg/kg AFB₁. Analyses of AFB₁-induced mutations showed high cosine similarity to SBS24, regardless of repair proficiency status. The absence of NEIL1 resulted in an approximately 30% increase in the frequency of mutations, with distribution suggesting preferential NEIL1-dependent repair of AFB₁ lesions in open chromatin regions. A trend of increased mutagenesis was also observed in the absence of XPA. Consistent with the role of XPA in transcription-coupled repair, mutational profiles in XPA-deficient mice showed disruption of the transcriptional bias in mutations associated with SBS24. Implications: Our findings define the roles of DNA repair pathways in AFB₁-induced mutagenesis and carcinogenesis in murine models, with these findings having implications in human health for those with BER and NER deficiencies.

Ecological Implications of Seasonal Variations in Physicochemical Parameters and Phytoplankton Community in an Effluent‐Receiving Wetland in the Gulf of Guinea, Cameroon

ABSTRACTPhysicochemical parameters, chlorophyll a (Chl a) concentration, and phytoplankton community structure were assessed in the Wouri estuarine mangrove sector of the Douala Bassa Industrial Zone of Cameroon over two seasons. Three sets of water samples were collected from 10 sites for these analyses. Some physicochemical parameters were measured in situ using multiparameter equipment (HANNA 8289). The data were subjected to analyses of significant difference by an independent sample t‐test. Correlation and principal component analysis conducted between variables Temperature, salinity, total dissolved solids (TDS), resistivity, and turbidity showed a strong seasonal variation among sites. Mean Chl a concentration (3.7–48.29 µg/L for dry and 0.1–20.1 µg/L for rainy season) revealed that most sites were eutrophic, with the Euglenophycean index less than 1 in all the sites. Phytoplankton species richness exhibited seasonal and spatial variation, with a total of 74 taxa and 51 taxa for the dry and rainy seasons, respectively. The division Bacillariophyta was the most abundant, with 19 families and 24 species during the dry season and 17 families and 16 species during the rainy season. Other phytoplankton divisions present included Chlorophyta &gt; Cyanophyta &gt; Charophyta &gt; &gt; Myzozoa &gt; Euglenophyta in the dry season; the less diverse divisions in the rainy season were Rhodophyta &gt; Cryptophyta &gt; Xanthophyta &gt; Euglenophyta. High concentrations of nutrients and the presence of eutrophic species such as Microcystis aeruginosa suggest pollution and a need to monitor activities carried out along the water course with respect to the quantity and quality of industrial effluents discharged into the stream. The observed eutrophication explains the noxious odor across the city of Douala, especially during the dry season. The human health implications could be severe, with communities dependent on this contaminated stream for fish and other necessities a scenario that repeats itself in communities dependent on urban wetlands in industrial zones globally.

Exploring expert perceptions towards emerging pollutants and their impacts in reused wastewater and agriculture

Water sustainability involves several natural and human spheres conditioning the availability and quality of water resources and life conservation. Regarding water quality, currently emerging pollutants (EPs) are a key topic at global scale since they are difficult to remove by traditional water treatment systems. In this context, this work aims to evaluate experts’ perception of several EPs issues with a special focus on semi-arid Mediterranean areas where EPs are negatively impacting water, environmental, and agricultural systems as, in these areas, effluents from water treatment plants are widely reused in the irrigation of crops, urban gardens, and golf courses or directly discharged on natural streams. Particularly, a detailed survey composed of questions about EPs regulation, risk insight, equipment, social and economic impacts, was performed collecting 437 responses. Main results suggested that EPs existence may pose a significant risk and a destabilizing factor in wastewater reuse, with negative impacts to crop irrigation, being managers the class with more concern followed by scientifics and administratives. New EPs regulations raises uncertainty amongst experts since 29 % considered positive its creation, while 20 % estimated this fact as regular and 14 % as negative. As well, although the combination of technologies to improve EPs removal generated agreement, aspects like the treatment charges at water treatment plants or the price/bill of EPs-free water were features of ambiguity. Within EPs elimination, the three expert groups highlighted that technologies impacts in the sustainability spheres will be positive on public health (87 % of responses), social trust (75 %), and environmental sustainability (76 %). Likewise, 88 % of experts concurred that the future of wastewater reuse relies on a combination of technologies. These findings offer valuable information to water legislators and policymakers to manage water resources, especially in semi-arid areas due to the final use of treated effluents and, therefore, the great implications for agriculture, environment and human health.

Investigating Exposure and Hazards of Micro- and Nanoplastics During Pregnancy and Early Life (AURORA Project): Protocol for an Interdisciplinary Study.

Micro- and nanoplastics (MNPs) are emerging pollutants of concern with ubiquitous presence in global ecosystems. MNPs pose potential implications for human health; however, the health impacts of MNP exposures are not yet understood. Recent evidence suggests that MNPs can cross the placental barrier, underlying the urgent need to understand their impact on reproductive health and development. The Actionable eUropean ROadmap for early-life health Risk Assessment of micro- and nanoplastics (AURORA) project will investigate MNP exposures and their biological and health effects during pregnancy and early life, which are critical periods due to heightened vulnerability to environmental stressors. The AURORA project will enhance exposure assessment capabilities for measuring MNPs, MNP-associated chemicals, and plastic additives in human tissues, including placenta and blood. In this interdisciplinary project, we will advance methods for in-depth characterization and scalable chemical analytical strategies, enabling high-resolution and large-scale toxicological, exposure assessment, and epidemiological studies. The AURORA project performs observational studies to investigate determinants and health impacts of MNPs by including 800 mother-child pairs from 2 existing birth cohorts and 110 women of reproductive age from a newly established cohort. This will be complemented by toxicological studies using a tiered-testing approach and epidemiological investigations to evaluate associations between maternal and prenatal MNP exposures and health perturbations, such as placental function, immune-inflammatory responses, oxidative stress, accelerated aging, endocrine disruption, and child growth and development. The ultimate goal of the AURORA project is to create an MNP risk assessment framework and identify the remaining knowledge gaps and priorities needed to comprehensively assess the impact of MNPs on early-life health. In the first 3 years of this 5-year project (2021-2026), progress was made toward all objectives. This includes completion of recruitment and data collection for new and existing cohorts, development of analytical methodological protocols, and initiation of the toxicological tiered assessments. As of September 2024, data analysis is ongoing and results are expected to be published starting in 2025. As plastic pollution increases globally, it is imperative to understand the impact of MNPs on human health, particularly during vulnerable developmental stages such as early life. The contributions of the AURORA project will inform future risk assessment. DERR1-10.2196/63176.

Assessment of Some Heavy Elements in Selected Fruits from the Markets of Anbar Governorate: Implications for Human Health

Eating fruit can be a useful way to enhance health and reduce the risk of many diseases. At the same time, if this fruit exceeds the permissible limit, it can be a source of diseases dangerous to human health. Therefore, this issue presents a complex and multifaceted challenge. The current study aims to assess the concentration of certain heavy metals (chromium, iron, nickel, copper, manganese) in the pulp and peels of selected fruits from the markets of Anbar Governorate, Iraq. Ten types of fruits were selected (quince, fig, orange, apple, banana, mango, watermelon, carrot, pomegranate, kiwi) and compared with internationally permissible limits. The concentrations were measured using an atomic absorption spectrometer. The results indicated the concentration of some metals exceeding limits of the international standard. For instance, it was noted that the concentration of Ni in the peels very high and the samples that exceeded the international limit were; A2 – B2 – C2 – K2 – M2 – P2 – S2 where samples had 6.548, 3, 3.645, 3.323, 17.516, 4.290 and 5.258 mg/L of Ni. In the pulp, nickel was found at high concentrations exceeding the international limits in samples (A1 - B1 - C1 - K1 - M1 - O1 - P1 - S1), with concentrations of (23.645 - 3.968 - 6.226 - 13.645 - 2.355 - 8.806 - 19.129) mg/L. The highest Ni was found in apple pulp at 23 mg/L while the lowest concentration of Ni was found in orange peel and fig pulp at 0.097 mg/L.

Impact of Indoor Air Pollution on Women’s Health: A Study on Sylhet City

Indoor air pollution is a significant and often overlooked environmental concern that has far-reaching implications for human health. In Bangladesh, indoor air pollution is one of the greatest environmental health risks. The study aims to provide insights into the specific ways in which indoor air pollution affects women’s health and well-being, as well as to identify potential strategies for mitigating these effects. With a focus on the diverse sources of indoor pollutants and their potential health effects. This qualitative research paper describes the impact of indoor air pollution on women’s health, focusing on the experiences, perceptions, and challenges faced by women in relation to their indoor living environments. This research used a qualitative approach, descriptive type research, purposive sampling strategy, and utilizing semi-structured interviews as the primary data collection method. A sample size of 50 women is targeted, with data saturation as the criterion for stopping data collection. Thematic analysis is used to analyze the interview transcripts. A critical analysis of the health effects of indoor air pollution on women is presented, encompassing consequences. The effects include exacerbation of respiratory conditions, allergies, eye and skin irritations. In conclusion, this research paper emphasizes the urgent need for a comprehensive understanding of the intricate relationship between indoor air pollution and women’s health. It underscores the importance of interdisciplinary collaboration among public health experts, environmental scientists, policymakers, and advocates to develop effective strategies that safeguard women’s well-being.

The Nexus between Polyphenols and Gut Microbiota and Their Interplay in Human Health: A Brief Review

Polyphenols are a broad class of naturally occurring substances in plants and have drawn extensive attention as they may possess promising health-promoting benefits. Recently, gut microbiota and polyphenol interactions have been directly linked to the well-being of humans. The classification, sources, and interactions of polyphenols with the gut microbiota are presented in this review, highlighting their key health benefits in humans. Polyphenols undergo complex transformations within the gastrointestinal tract and interact with the gut microbiota, a varied collection of bacteria living in the digestive system. The interactions substantially influence the composition, functioning, metabolic activity, and gut microbiota diversity. Research indicates that polyphenols may possess prebiotic-like properties, favouring Lactobacilli and Bifidobacteria growth, among other beneficial bacteria. The fermentation of polyphenols is aided by these bacteria, which produce bioactive metabolites that may improve human health and well-being in various ways. Moreover, the alteration of gut microbiology caused by polyphenols has been linked to improvements in several health outcomes, including enhanced metabolic health, fortified immunological function, and a decreased susceptibility to chronic conditions like heart disease and certain forms of cancer. In summary, the intriguing relationship between polyphenols and gut microbiota has significant health implications for humans. Understanding these relationships can open the door to tailored dietary treatments and the development of functional foods to support a balanced gut microbiota and general well-being.

Comparative analysis of CNN and TNN models in environmental noise source identification

This paper presents a focused examination of environmental noise, an issue of relevance due to its implications for human health and well-being, compliant with ISO 1996-2:2017 standards. Moving beyond the traditional methodologies that primarily rely on level exceedance, our study integrates advanced machine learning techniques to address the challenges in noise source identification. With the advent of IoT Noise Monitoring Terminals, the conventional manual auditory analysis methods have become less feasible. Our research explores the application of convolutional neural networks (CNNs), a standard in environmental noise analysis, and assesses the emerging utility of Transformer Neural Network (TNN) models in this domain. The aim is to conduct an objective comparison of these models, applying them to identical datasets to determine their effectiveness in identifying noise sources. Through this analysis, the study seeks to contribute to the field of environmental acoustics by offering insights into the comparative strengths and limitations of CNN and TNN models.