Impatiens Balsamina Research Articles

First report of Ludwigia yellow vein Vietnam virus causing leaf curling on tobacco plants in Hainan province, China.

Cigar tobacco (Nicotiana tabacum L.) has been recently introduced into China for various industrial applications. From March 2022, certain symptoms of begomovirus infection, including leaf curling and thickening of veins, were sporadically (disease incidence was approximately 0.2%) observed in several cigar tobacco plantations in numerous counties in Hainan Province, China (Figure 1A). These typical symptoms of begomovirus infection were similar to those caused by the sida leaf curl virus-Hainan (SiLCV-HN) begomovirus and its associated betasatellite, as reported in our previous study on cigar tobacco plants in the same region (Wang et al. 2022). In order to determine whether these symptoms were caused by SiLCV-HN or other begomoviruses, samples of leaves were collected from the diseased tobacco plants for DNA extraction, and the total DNA was extracted for viral metagenomics using an Illumina Sequencing platform at Tiangen Biotech, Beijing. A total of 65711396 filtered reads were obtained, of which 65362322 (99.47%) reads matched to the genome of tobacco. The remaining unmapped 349074 (0.53%) reads were analyzed by BLASTn against the virus Refseq Database of GenBank and subsequently assembled. A total of 8 (5+2+1) enriched contigs of the complete sequence of ludwigia yellow vein Vietnam virus (LuYVVNV) and 9 (8+1) contigs of ludwigia yellow vein virus-associated DNA beta (LuYVB) were finally obtained (Table 1). LuYVVNV belongs to the Begomovirus genus that infects various weeds, including Ludwigia octovalvis and Impatiens balsamina. As far as we know, it was reported earliest on weed in Vietnam (Ha et al. 2008). GenBank contains data pertaining to previously identified isolates of LuYVVNV, and the data revealed that the virus was discovered in Vietnam and the Yunnan province of China currently. However, there are no reports on the infection of crops by LuYVVNV to date. The findings of the present study indicated that LuYVVNV and LuYVB could be responsible for the aforementioned symptoms observed on cigar tobacco. The complete genomes of LuYVVNV and LuYVB were amplified using primer pairs designed based on sequence assembly for viral metagenomics (Table 2). Indeed, two DNA bands with length 2763 bp of LuYVVNV genome and 1348 bp of LuYVB were amplified from leaf samples of diseased tobacco (Figure 1B). The products of polymerase chain reaction (PCR) amplification were analyzed by Sanger sequencing, and the complete nucleotide sequences of LuYVVNV and its associated betasatellite were obtained. Analysis with the BLASTn tool of NCBI revealed that the genome sequence of LuYVVNV isolated from the Hainan province of China had the highest identity of 96.9% to a different isolate of LuYVVNV (GenBank accession number: MN210347.1). These two isolates belong to the same strain, according to the latest revision of Begomovirus taxonomy (Brown et al. 2015). The isolate of LuYVVNV identified in this study was designated as LuYVVNV, Hainan isolate (LuYVVNV-HN, GenBank accession number: OP948731). BLASTn analysis further revealed that the associated betasatellite had the highest sequence identity of 96.9% with an LuYVB (GenBank accession number: AJ965541.1) of a different viral isolate, according to the classification and nomenclature of DNA betasatellites of begomoviruses (Briddon et al. 2008). The sequence of LuYVB obtained herein was therefore designated as LuYVB, Hainan isolate (LuYVB-HN, GenBank accession number: OP948732). The pathogenicity of LuYVVNV-HN and LuYVB-HN was determined using infectious clones that were constructed by ligating two fragments of LuYVVNV-HN or LuYVB-HN to a binary pCAMBIA1300 expression vector, as previously described (Wang et al. 2019). Infectious clones of LuYVVNV-HN, LuYVB-HN, and LuYVVNV-HN plus LuYVB-HN were separately agroinfiltrated into N. benthamiana for determining viral pathogenicity. The typical symptoms of begomovirus infection were observed in N. benthamiana plants inoculated with LuYVVNV-HN alone or LuYVVNV-HN plus LuYVB-HN, and the emerging leaves were mildly or severely down-curled, respectively, at 7 days post inoculation (dpi), with 100% disease incidence (6/6) (Figure 1C). Positive PCR products of the AV1 gene of LuYVVNV-HN were obtained from N. benthamiana plants inoculated with LuYVVNV-HN alone or LuYVVNV-HN plus LuYVB-HN. The βC1 gene of LuYVB-HN was only obtained from N. benthamiana plants co-infected with LuYVVNV-HN and LuYVB-HN (Figure 1D). No symptoms of viral infection were observed in plants individually inoculated with LuYVB-HN, and the results of PCR were negative (Figure 1C and 1D). These findings indicated that the N. benthamiana plants had been successfully inoculated with LuYVVNV-HN, and that LuYVB-HN was incapable of causing infections on its own, but functioned as a helper and enhanced viral pathogenicity. This report is the first to identify isolates of LuYVVNV and LuYVB from cigar tobacco, which is an economically important crop plant. The findings provide insights into the epidemic threat of begomovirus reservoirs in weeds to crop plants, and emphasize the need for monitoring and controlling whitefly-transmitted viral diseases in tobacco plantations worldwide (Ye et al. 2021).

Impatient Balsamina as Agribusiness Commodity in Bali

Impatient balsamina is one of the agricultural commodities that is mass-cultivated on the island of Bali, and the use of inpatient balsamina flowers as religious and cultural facilities in the community in Bali. So that the impatient balsamina agribusiness community is able to provide a decent income for farmers and marketing institutions, the purpose of the research is to find out the efficiency of impregnated balsamina marketing in Bali and to know the impatient balsamina marketing channel. Agribusiness impatiens balsamina becomes one of the interesting phenomena to be studied because marketing is mostly marketed in traditional markets in big cities in Bali such as in the traditional markets of Denpasar. The main consumers of impatient balsamina are urban people living in cities on the island of Bali. The results showed that the elasticity of marketing transmission of impatient balsamina commodities amounted to 1,143, this shows if Et> 1, then the rate of change in prices at the producer level. The market faced by all market participants is imperfect actors, that is, there is monopoly and oligopoly power in the marketing system and the marketing system is not yet efficient. Impatient balsamina production give marketing channels are farmers, traders, wholesalers, retailers, consumers

Plant Antimicrobial Peptides: Insights into Structure-Function Relationships for Practical Applications

Antimicrobial peptides (AMPs) are short polypeptide molecules produced by multicellular organisms that are involved in host defense and microbiome preservation. In recent years, AMPs have attracted attention as novel drug candidates. However, their successful use requires detailed knowledge of the mode of action and identification of the determinants of biological activity. In this review, we focused on structure-function relationships in the thionins, α-hairpinins, hevein-like peptides, and the unique Ib-AMP peptides isolated from Impatiens balsamina. We summarized the available data on the amino acid sequences and 3D structure of peptides, their biosynthesis, and their biological activity. Special attention was paid to the determination of residues that play a key role in the activity and the identification of the minimal active cores. We have shown that even subtle changes in amino acid sequences can affect the biological activity of AMPs, which opens up the possibility of creating molecules with improved properties, better therapeutic efficacy, and cheaper large-scale production.

Evidence on temperature and concentration of reducing agents to control the nanoparticles growth and their microbial inhibitory efficacy

The present study deals with the nanoparticles synthesis from Impatiens balsamina L. plant flower extract. The concentration of reducing agent (flower extract) and different temperature is involved in the reaction. Sixteen different silver nanoparticles were synthesized with using different ratios of the reaction mixture and different temperature. The different silver nanoparticles are different color based on the temperature and concentration of reaction mixture. The synthesized silver nanoparticles are characterized by UV/vis Spectrophotometer. The microorganisms Bacillus subtilis are highly inhibited by (90:10/60 °C; 99:1/70 °C; 92:2/70 °C; and 90:10/70 °C) silver nanoparticles. Pseudomonas aeruginosa are highly inhibited by 98:2/50 °C and 90:10/70 °C based silver nanoparticles. The bacterial species Staphylococcus aureus are highly inhibited by 98:2/50 °C and 98:2/70 °C. The E. coli was inhibited by 99:1/70 °C; 98:2/70 °C and 90:10/70 °C based silver nanoparticles. The temperature and concentration of reducing agents can play a significant role in controlling the growth of nanoparticles. This study is one of the evidence on temperature and concentration of reducing agents to control the nanoparticles growth and their microbial inhibitory efficacy.

Protective effects of MNQ against Lipopolysaccharide-induced inflammatory damage in bovine ovarian follicular granulosa cells in Vitro

Inflammation of the reproductive tract in dairy cows lead to functional disorders of follicular granulosa cells (GCs) in mammalian ovaries resulting in infertility and serious losses to the livestock industry. Lipopolysaccharide (LPS) can induce an inflammatory response in follicular granulosa cells in vitro. The aim of this study was to investigate the cellular regulatory mechanism of MNQ (2-methoxy-1,4-naphthoquinone) on eliminating the inflammatory response and restoring normal functions for bovine ovarian follicular GCs cultured in vitro exposed to LPS. The cytotoxicity of MNQ and LPS on GCs were detected by MTT method to determine the safe concentration. The relative expression of inflammatory factors and steroid synthesis-related genes were detected by qRT-PCR. The concentration of steroid hormones in the culture broth were detected by ELISA. Differential gene expressions were analyzed by RNA-seq. There were no toxic effects on GCs at MNQ and LPS concentrations of less than 3 µM and 10 µg/mL, respectively and treated in 12 h. The relative expressions of IL-6, IL-1β and TNF-α were significantly higher in the LPS group compared with the CK group when GCs cultured in vitro were treated with the above concentrations and times (P < 0.05), but significantly lower in the MNQ+LPS group compared with the LPS group (P < 0.05). The levels of E2 and P4 in the culture solution were significantly reduced in the LPS group compared to the CK group (P < 0.05), and restored in the MNQ+LPS group. The relative expressions of CYP19A1, CYP11A1, 3β-HSD, and STAR were significantly decreased in the LPS group compared with the CK group (P < 0.05), while the MNQ+LPS group also recovered to some extent. There were 407 differential genes shared by LPS vs CK and MNQ+LPS vs LPS by RNA-seq analysis, which were mainly enriched in steroid biosynthesis and TNF signaling pathway. We screened 10 genes for analysis and found consistent results for RNA-seq and qRT-PCR. In this study, we confirmed the protective effect of MNQ, an extract from Impatiens balsamina L, on LPS-induced inflammatory responses in bovine follicular granulosa cells in vitro as well as functional damage, and acted through steroid biosynthesis and TNF signaling pathways.

Characterization of Plant Growth Promoting Rhizobacteria From Seasonal Flower Beds

The present investigations were carried out to screen the PGPR isolates from five separate seasonal flower beds (Catharanthus roseus, Portulaca grandiflora, Celosia argentea, Gomphrena globosa and Impatiens balsamina) of Curzon Hall, University of Dhaka. The bacterial load of the collected soil samples ranged in between 7.60 ± 6.01 × 106 and 9.49 ± 5.44× 108 cfu/g, 5.34 ± 4.06×106 and 13.4 ± 4.8×106 cfu/g and 1.01 ± 0.59×106 and 1.19 ± 0.95×108 cfu/g on Nutrient Agar (NA), Luria-Bertani (LB) and Yeast Extract Mannitol Agar (YEMA), respectively. All the selected eight isolates were Gram positive and rod shaped. All isolates were found to be IAA producer and 7 showed varying levels of phosphate solubilizing activity. IAA production and phosphate (P) solubilization of the selected isolates ranged in between 9.76 ± 5.98 to 17.99 ± 1.865 U/ml, and 1.17 ± 0.59 to 1.84 ± 0.499 mg/l. Maximum production of both IAA and phosphate solubilization was achieved in 24h of incubation period at 37°C and pH 7.0. Germination test against Mung Bean (Vigna radiata) revealed positive plant growth promoting activities and could be used as PGPR inoculant. Dhaka Univ. J. Biol. Sci. 31(2): 321-328, 2022 (July)

EFEKTIVITAS EKSTRAK METANOL DAUN KETEPENG CINA (Cassia alata L.) DALAM MENGHAMBAT PERTUMBUHAN BAKTERI Staphylococcus epidermidis SECARA TUNGGAL DAN KOMBINASI

The goal of this study was to determine the antibacterial activity and combined effect of a 10% w/v methanol extract of ketepeng cina leaves (Cassia alata) with six selected plant extracts against Staphylococcus epidermidis using the diffusion and paper strip methods. The results revealed that the average antibacterial activity of each extract inhibited the growth of S. epidermidis. The inhibition zones of single extract of C. alata, Excocaria cochinchinensis, Ocinum tenuiflorum, Persea americana, Oroxylum indicum, Impatiens balsamina, and Peronema canescens were 6.28±0.20 mm, 7.90±0.31 mm, 2.30±0.37 mm, 3,53±0.50 mm, 4.35±0.52 mm, and 4.69±0.17 mm, respectively. Furthermore, the combination of ketepeng cina leaves (C. alata) methanol extract with the six selected plants extract in an average diameter of inhibition zone greater than the average diameter of inhibition zone of each extract. However, the combined extract's average diameter of the inhibition zone was not significantly larger than the sum of the diameters of the inhibition zones of each extract. According to the findings of this study, combining the methanol extract of C. alata with six selected plant extracts has an additive/indifferent effect on the growth of S. epidermidis.

Phytochemical and pharmacological potential of Impatiens balsamina

Impatiens balsamina is an annual herb, belongs to the family Balsaminaceae, commonly seen in India, China, and Korea. It is an erect plant commonly known as rose balsam and garden balsam. Studies shows that the plant contain many valuable phytoconstituents such as flavonoids, saponins, phenolics, naphthoquinones, and glycosides. The plant is used in traditional medicine for their antimicrobial, anti-inflammatory, anti-allergic and antidermatitic properties. The plant can also be used to treat burns, scalds, and lumbago. Flowers of the plant is used as dye in pastry. Seeds of this plant is edible. Leaves and stem are also edible when boiled. Leaves, stem, flowers are also having medicinal properties. Microscopical features of pollen grains shows that the pollen grains contain a vegetative and generative cell. Many studies have been done to identify the pharmacological properties of this plant. Some of the pharmacological actions of the plant include antipruritic, antidermatitic, antimicrobial, antitumor, wound healing, antidiabetic and antinociceptive. The present review summarizes information about the morphology, chemical constituents, and pharmacological actions of Impatiens balsamina for future works.

Phytochemical and pharmacological potential of Impatiens balsamina

Phytochemical and pharmacological potential of Impatiens balsamina

Karakteristik Komunitas Serangga Pengunjung Bunga Pada Beberapa Tanaman Refugia

This study was conducted to determine the characteristics of the insect community of flower visitors on several refugia plants. The research was carried out in the experimental field of the Faculty of Agriculture, Jambi University and the Laboratory of Plant Pests, Faculty of Agriculture, Jambi University. Samples of flower-visiting insects were taken using the yellow pan trap, pitfall trap, and sweep trap. The insects obtained were identified in the Laboratory of the Faculty of Agriculture, Jambi University. Characteristics of insect communities in refugia plants were calculated using the formulas of diversity index, similarity index, dominance index, and similarity index. The results showed that the abundance of species and populations of insects was most commonly found in Zinnia elegans flowers. Most predatory insects were found in Z. elegans flowers, pollinator insects were most abundant in Helianthus annuus flowers, and pollinators were most abundant in Z. elegans flowers. The characteristics of the insect community in the most stable refugian plant were found in Tagetes erecta flowers. The highest similarity of insect communities was found in Cosmos caudatus flowers and Impatiens balsamina flowers.

CHEMICAL COMPOSITION AND BIOLOGICAL ACTIVITY OF SECONDARY METABOLITES FROM IMPATIENS BALSAMINA

The review summarizes the literature data concerning the chemical composition of secondary metabolites and the types of biological activity of extracts and separate groups of secondary metabolites of Impatiens balsamina. First, data are given concerning the different types of biological activity of the extracts. Further, individual groups of secondary metabolites are considered, the corresponding structural formulas and types of biological activity established for this group of secondary metabolites are given. An attempt has been made to present the material about chemical composition and types of biological activity in chronological order. Extracts of I. balsamina have been shown to exhibit antiallergic, antihypotensive, antitumor, antinociceptive, antioxidant, antirheumatoid, antimicrobial, and antifungal activities. Among the secondary metabolites, peptides, naphthoquinones, polysaccharides, saponins, flavanoids, polyphenols, and tetrahydronaphthalene derivatives were identified. Research on peptides with a broad spectrum of antimicrobial activity is perspective. One of the most important groups of secondary metabolites are naphthoquinones, among which 2-methoxy-1,4-naphthoquinone is a significant metabolite, with which the antitumor effect of I. balsamina is associated. Also, this substance has shown in a number of tests an antifungal and antimicrobial activity exceeding the reference drug. Neuroprotective activity is simultaneously associated with a number of representatives of saponins, flavanoids, phenylpropanoids and tetrahydronaphthalene derivatives. This review shows that I. balsamina contains many groups of secondary metabolites, for which different types of biological activity have been identified. Due to the fact that the discussed plant is widely cultivated and is available, I. balsamina is a perspective object for the creation of new effective drugs.

In Vitro Antimicrobial Potential of Impatiens Balsamina Flower Against Staphylococcus Aureus, Escherichia Coli and Candida Albicans

The search for new drugs to treat cases of infection continues. This is because the microorganisms that cause infection continue to mutate as a form of self-defense which causes resistance to antibiotics. One strategy for finding new drugs is through the exploration of active ingredients derived from plants that have been used empirically by the community. Impatiens balsamina is a plant that has been shown to have antibacterial, antifungal, antipruritic, and anti-allergic characteristics. The ethanol extract of the Impatiens balsamina flower contains secondary metabolites such as naphthoquinones, coumarin derivatives, flavonoids, steroids, quinones, and saponins. This study aimed to obtain the active ingredient of the Impatiens balsamina flower which is used as an herbal medicine in the treatment of infection. A multilevel extraction process was carried out using solvents of different polarity, so that fractions containing nonpolar compounds, semipolar compounds, and polar compounds were obtained. Then, each fraction was tested for antimicrobial potency. Antimicrobial testing in vitro was carried out using the disc diffusion method. The results showed that the secondary metabolites contained in the hexane fraction were terpenoids, flavonoids, polyphenols, and anthraquinone compounds. Secondary metabolites contained in the ethyl acetate fraction were alkaloids, terpenoids, flavonoids, polyphenols, and anthraquinone compounds. Secondary metabolites contained in the ethanol fraction were terpenoids, flavonoids, polyphenols, and anthraquinone compounds. The hexane fraction had the best percentage of inhibition and percentage of effectiveness against Staphylococcus aureus, Escherichia coli, and Candida albicans.&#x0D; Keywords: antimicrobial, fractionation, Impatiens balsamina L flower, disc diffusion

Wide diversity of pollens in honey samples from Indian Himalayan neighbourhoods: a melissopalynological study

Melissopalynological analysis of 34 honey samples (17 summer and 17 autumn samples) collected from feral colonies of Indian hive bee (Apis cerana), European bee (Apis mellifera) and rock bee (Apis dorsata) during 2011 – 2015 from the Shimla Hills of Himachal Pradesh (India) was performed. Pollen from plant taxa belonging to 43 families was found in the honey samples. Of 34 honey samples from the Shimla Hills, 18 were unifloral with 12 pollen types as predominant and the remaining 16 samples were multifloral. The predominant pollen plant taxa of summer were Pyrus sp., Malus domestica, Prunus amygdalus, Prunus sp., Eucalyptus camaldulensis, Citrus sp. and Rhododendron arboreum. In autumn, the predominant pollen plant taxa were Leucaena leucocephala, Salvia officinalis, Eriobotrya japonica, Impatiens balsamina and Prinsepia utilis. Melissopalynological analysis of summer honey collected from Summer Hill and Sarahan tagged these as “mad honey”, since Rhododendron pollen was predominant. The pollen spectrum indicated a diverse bee plant flora, which is promising indicator to augment the beekeeping industry in this region.

Molecular characterization of a putative alphapartitivirus from Impatiens balsamina L.

Two double stranded RNAs (dsRNAs) that likely represent the genome of an alphapartitivirus, tentatively named "impatiens cryptic virus 1" (ICV1), were recovered from Impatiens balsamina L. RNA1 (2008 bp) codes for the RNA-dependent RNA polymerase (RdRp) of ICV1, which shares <83% amino acid sequence identity with the RdRps of other alphapartitiviruses. RNA2 (1906 bp) codes for the coat protein (CP) of ICV1, which shares <60% amino acid sequence identity with the CPs of other alphapartitiviruses. Phylogenetic analysis suggested that ICV1 is closely related to plant alphapartitiviruses, including vicia cryptic virus, beet cryptic virus 1, carrot cryptic virus, and white clover cryptic virus 1. Using primers specific for RNA1 or RNA2, ICV1 could be detected in I. balsamina from various parts of China.

Multi-Objective Optimization Using Cooperative Garden Balsam Optimization with Multiple Populations

Traditional multi-objective evolutionary algorithms (MOEAs) consider multiple objectives as a whole when solving multi-objective optimization problems (MOPs). In this paper, the hybridization of garden balsam optimization (GBO) is presented to solve multi-objective optimization, applying multiple populations for multiple objectives individually. Moreover, in order to improve the diversity of the solutions, both crowding distance computations and epsilon dominance relations are adopted when updating the archive. Furthermore, an efficient selection procedure called co-evolutionary multi-swarm garden balsam optimization (CMGBO) is proposed to ensure the convergence of well-diversified Pareto regions. The performance of the used algorithm is validated on 12 test functions. The algorithm is employed to solve four real-world problems in engineering. The achieved consequences corroborate the advantage of the proposed algorithm with regard to convergence and diversity.

Theoretical analysis of garden balsam optimization algorithm

Garden balsam optimization (GBO) is a new proposed evolutionary algorithm based on swarm intelligence. Convergence and time complexity analyses are very important in evolutionary computation, but the research on GBO is still blank. Same as other evolutionary algorithms, the optimization process of the GBO algorithm can be regarded as a Markov process. In this paper, a Markov stochastic model of the GBO algorithm is defined and used to prove the convergence of GBO algorithm. Finally, the approximation region of the estimated convergence time of GBO algorithm is calculated, which characterizes the evolution of the evolutionary process of the proposed algorithm.

ANTIBACTERIAL AND ANTIFUNGAL ACTIVITIES OF Impatiens balsamina (L.): LITERATURE REVIEW ARTICLE

The human body contains a diverse range of microorganisms, including bacteria and fungi. The most common bacteria and fungi caused skin pathogens are Staphylococcus aureus, Propionibacterium acnes, Staphylococcus epidermis and Candida albicans. Many studies have found that Impatiens balsamina (L.) contains antibacterial and antifungal activities. Acne and Candidiasis are diseases that often arise and can interfere with comfort for sufferers. Usually, acne is caused by the bacteria Propionibacterium acnes, Staphylococcus epidermis and Staphylococcus aureus, and Candidiasis caused by the Candida albicans. One of the plants that is widely used as an antiacne and antifungal treatment is the Impatiens balsamina (L.). Impatiens balsamina (L.) has antiacne and antifungal activities. The purpose of the review is to find out if the Impatiens balsamina (L.) has activity as an antiacne, antifungal and can identify secondary metabolites that can inhibit the emergence of bacteria in acne and candidiasis in various journals. The method used is the Literature Review Article (LRA) method. A search through Google Scholar and Pubmed found 12 articles that Impatiens balsamina (L.) contains secondary metabolites, namely flavonoids and saponins. The mechanism of action of flavonoids is by inhibiting the function of cell membranes and energy metabolism of bacteria, while the mechanism of action of saponins as antifungals is by damaging cell membranes, causing cell leakage which ultimately leads to cell death. From the two mechanisms, these secondary metabolites can have antiacne and antifungal activities.

Complete Chloroplast Genomes and Comparative Analyses of Three Ornamental Impatiens Species

Impatiens L., the largest genus in the family Balsaminaceae with approximately 1,000 species, is a controversial genus. Due to the conflict of morphological features and insufficient genomic resources, the studies of systematic evolution and understanding of taxonomic identification are considered to be very limited. Hence, we have sequenced the complete chloroplast genomes of three ornamental species (Impatiens balsamina, I. hawkeri, and I. walleriana), and compared them with previously published wild species data. We performed a detailed comparison of a highly similar basic structure, size, GC content, gene number, order, and functional array among them. Similarly, most divergent genes were detected from previous work in the literature. The mutational regions containing highly variable nucleotide hotspots were identified and may be used as potential markers for species identification and taxonomy. Furthermore, using whole chloroplast genome data to analysis the phylogenetic relationship of the Balsaminaceae species, we found that they were all part of a single clade. The three phenotypically different ornamental species were clustered together, suggesting that they were very likely to be closely related. We achieved and characterized the plastid genome structure, identified the divergence hotspots, and determined the phylogenetic and taxonomic positions of the three cultivated species in the Impatiens genus. The results may show that the chloroplast genome can be used to solve phylogenetic problems in or between the Impatiens genus and also provide genomic resources for the study of the Balsaminaceae family’s systematics and evolution.

Distinct kin strategies of the legume soybean and the non-legume balsam by accomplishing different nitrogen acquisition and rhizosphere microbiome composition.

SUMMARYKin selection has been proposed vvto be an important mechanism for plant relatives growing together. To reveal kin recognition, we used15N labeling to assess the short‐term nitrogen (N) acquisition (uptake of nitrate and ammonium), long‐term N utilization (recovery of added urea), N‐use efficiency (NUE) and rhizosphere microbiome in leguminousGlycine maxand non‐leguminousImpatiens balsamina. Individuals of each species were planted pairwise with either a sibling or a stranger. Enzyme activity and soil microbial composition were compared between kinship groups. Compared with strangers,G. maxsiblings increased aboveground biomass, NUE, and nitrogenase activity, whereasI. balsaminasiblings decreased root biomass and increased uptake rate of nitrate and potential nitrification rate. Plant kinship affected soil bacterial communities by enriching specific groups possessing explicit eco‐functions (Rhizobiales forG. maxandNitrospiraforI. balsamina). Kinship‐sensitive operational taxonomic units formed independent modules in the bacterial co‐occurrence network and were positively correlated with plant growth performance, N acquisition and enzymatic activity. Plant kin recognition may depend on the growth strategies of the plant species. Kin selection was dominant inG. maxby enhancing biological N fixation through the enrichment of symbiotic rhizobia (demonstrated by aboveground growth and NUE superiority among siblings). Kin selection and niche partitioning occurred simultaneously inI. balsamina, expressed through reduced root allocation but increased nitrate uptake, and enhanced soil N nitrification, by enriching functional microbial groups. Kin recognition responses are the consequence of complex interactions among the host plant, the microbiome, and soil nutrient cycling and utilization processes.

Quick-identification of total phenolic and flavonoid content of Mangifera foetida, Lagerstroemia speciosa and Impatiens balsamina gathered from Riau, Sumatera

Introduction: Variance in a species and climate conditions often impact the phytochemical content and pharmacological properties of many medicinal plants. In this study, we used simple and quick methods to determine the total phenolic and flavonoid contents in ethanol extracts from three species of medicinal plants from Riau, Sumatera. Results obtained were then compared to existing literature. Materials and Methods: Several parts of plant were used, namely Mangifera foetida bark (MB), pericarp (MP), leaves (ML), Lagerstreomia speciosa bark (LB), flower (LFlo), leaves (LL), fruit (LFru), and Impatiens balsamina leaves (IL), root (IR), and flower (IFlo). Total phenolic content was estimated using Folin-Ciocalteau method, while flavonoid content was measured using common colorimetric method. Results and Discussion: Results obtained in this study indicate that LL has the highest flavonoid content (22.116 ± 0.409 mg QE/g) while MB contains the highest phenolic content (24.642 ± 11.087 mg GAE/g) amongst the different samples used in this study. Conclusion: When compared to existing literatures, results presented here indicate that plants from Riau, Sumatera are potential sources of pharmacologic ingredients as indicated by the presence of high flavonoid and phenolic content. Further pharmacological study on specific mechanisms of action from active substances isolated from LL, MB, and IL is required.