Wastewater reuse is a non-conventional source of water containing high amounts of nutrients but also other undesirable components. However, little is known about the impact of wastewater irrigation on farmland soil properties, particularly in Afghanistan. Therefore, this paper reports for the first time a comprehensive understanding of the presence and effect of soil properties under wastewater irrigation and freshwater irrigation in Kandahar, Afghanistan. Accordingly, the properties of soil were evaluated by collecting soil samples from the sites of Haji-Arab (HA), Bala-Karz (BKZ), Mahal-e-Nejat (MN), and Char-Bagh (CB) in Kandahar province. The agricultural fields at the HA, BKZ, and MN sites are irrigated with wastewater, while at the CB site farms are entirely irrigated by freshwater. The results of this study revealed that the lowest pH and maximum EC levels were recorded in the BKZ soil samples, followed by the HA, MN, and CB soil samples. The soil texture class was silt loam and sandy loam at the wastewater-irrigated and freshwater-irrigated sites, respectively. Discoveries from this study have identified implications for informing policymakers on the need for appropriate wastewater treatment systems and regulations to ensure the safe and sustainable use of wastewater for irrigation purposes.