Impact Of Iron Oxide Nanoparticles Research Articles

Role of iron oxide nanoparticles in maize (Zea mays L.) to enhance salinity stress tolerance

Soils have been getting worse over time, which has led to lower crop yields and nutritional value. This is because of too many conventional fertilizers, anthropogenic activities, and climate change. Soil salinity is also a big problem and challenge for agricultural scientists. To address this issue, nanoparticles are gaining a reputation in agriculture that can enhance salinity tolerance in crops, especially at early growth stages. A pot experiment was conducted at Post Agricultural Research Station (PARS), Faisalabad to assess the impact of iron oxide nanoparticles (0, 15, and 30 ppm) and four levels of salinity (0, 50, 100 and 150 mM) on morphological, physiological and yield traits of maize (Zea mays L.) in salinity stress. Salinity stress significantly negatively affected the growth attributes, photosynthetic pigments, ion content (Na+, K+, and Ca2+) of maize plants. Salinity has also increased the levels of MDA, H2O2, and Na+ ions. The application of iron oxide nanoparticles through foliar spray had a notable impact in enhancing the growth and yield of the tested maize variety. It was achieved by promoting the activities of antioxidant enzymes, increasing photosynthetic pigments, and elevating K+ and Ca2+ ion levels under both normal and salinity-stressed conditions. Additionally, iron oxide nanoparticles mitigated the adverse effects of salinity stress by effectively reducing Na+ ion concentration, MDA levels, and H2O2 concentration. Among the different concentrations tested, 30 ppm of iron oxide nanoparticles proved best in alleviating the negative impacts of salinity stress in maize. Thus, field use of 30 ppm iron oxide nanoparticles as foliar spray could effectively mitigate salinity stress in maize.

Impact of Iron Oxide Nanoparticles on Anaerobic Co-digestion of Chicken Manure and Sewage Sludge Substrates

Impact of Iron Oxide Nanoparticles on Anaerobic Co-digestion of Chicken Manure and Sewage Sludge Substrates

Unveiling the effect of gibberellin-induced iron oxide nanoparticles on bud dormancy release in sweet cherry (Prunus avium L.)

Hydrogen cyanide has been extensively used worldwide for bud dormancy break in fruit trees, consequently enhancing fruit production via expedited cultivation, especially in areas with controlled environments or warmer regions. A novel and safety nanotechnology was developed since the hazard of hydrogen cyanide for the operators and environments, there is an urgent need for the development of novel and safety approaches to replace it to break bud dormancy for fruit trees. In current study, we have systematically explored the potential of iron oxide nanoparticles, specifically α-Fe2O3, to modulate bud dormancy in sweet cherry (Prunus avium). The synthesized iron oxide nanoparticles underwent meticulous characterization and assessment using various techniques, including Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), transmission electron microscopy (TEM), and ultraviolet–visible infrared (UV–Vis) spectroscopy. Remarkably, when applied at a concentration of 10 mg L−1 alongside gibberellin (GA4+7), these iron oxide nanoparticles exhibited a substantial 57% enhancement in bud dormancy release compared to control groups, all achieved within a remarkably short time span of 4 days. Our RNA-seq analyses further unveiled that 2757 genes within the sweet cherry buds were significantly up-regulated when treated with 10 mg L−1 α-Fe2O3 nanoparticles in combination with GA, while 4748 genes related to dormancy regulation were downregulated in comparison to the control. Moreover, we discovered an array of 58 transcription factor families among the crucial differentially expressed genes (DEGs). Through hormonal quantification, we established that the increased bud burst was accompanied by a reduced concentration of abscisic acid (ABA) at 761.3 ng/g fresh weight in the iron oxide treatment group, coupled with higher levels of gibberellins (GAs) in comparison to the control. Comprehensive transcriptomic and metabolomic analyses unveiled significant alterations in hormone contents and gene expression during the bud dormancy-breaking process when α-Fe2O3 nanoparticles were combined with GA. In conclusion, our findings provide valuable insights into the intricate molecular mechanisms underlying the impact of iron oxide nanoparticles on achieving uniform bud dormancy break in sweet cherry trees.

Iron oxide nanoparticles induced cytotoxicity, oxidative stress, cell cycle arrest, and DNA damage in human umbilical vein endothelial cells

BackgroundNanotechnology and material science have developed enormously fast in recent years. Due to their excellent magnetic properties, iron oxide nanoparticles (IONPs) have been broadly applied in the field of bioengineering and biomedical. Thus, it is important to evaluate the safety issues and health effects of these nanomaterials. The present investigation was aimed to evaluate the adverse effects of IONPs on human umbilical vein endothelial cells (HUVECs). MethodsThe cytotoxic potential of IONPs was assessed by MTT and neutral red uptake (NRU) assays. The impact of IONPs on oxidative stress markers (glutathione (GSH) and lipid peroxidation (LPO)), reactive oxygen species (ROS) production, and mitochondrial membrane potential (MMP) was also examined. Furthermore, the toxic effect of IONPs was quantified by assessing DNA damage, cell cycle arrest, and apoptosis by quantitative real time PCR. ResultsWe found that IONPs induce a dose-dependent cytotoxicity on HUVECs with IC50 value of 79.13 μg/mL. The results also displayed that IONPs induce oxidative stress, ROS production, and mitochondrial membrane dysfunction. The comet assay results exhibited IONPs induces DNA damage in HUVECs. We found significant cell cycle arrest at SubG1 phase in treated cells and consequent cell death was evidenced by microscopic analysis. Moreover, IONPs display substantial up-regulation of pro-apoptotic genes and down-regulation of anti-apoptotic gene evidenced by real time qPCR. ConclusionOverall, our results clearly demonstrated that IONPs have the potential to induce cytotoxicity, DNA damage, cell cycle arrest, and apoptosis in HUVECs mediated through oxidative stress and ROS production. Thus, IONPs are cytotoxic and it should be handled with proper care.

Impact of Iron Oxide Nanoparticles on Anaerobic Co-Digestion of Cow Manure and Sewage Sludge

Supplementation with iron oxide nanoparticles has been suggested as a potential method for improving energy generation through anaerobic digestion, specifically by enhancing the rate of methane production. This investigation examined the effects of iron oxide (Fe3O4) nanoparticles (NPs) on anaerobic co-digestion of cow manure (CM) and sewage sludge (SS) through batch testing conducted under mesophilic conditions (35 °C) using a RESPIROMETRIC Sensor System 6 Maxi—BMP (RSS-BMP). The use of Fe3O4 nanoparticles at doses of 40, 80, 120, and 160 mg/L (batches M1, M2, M3, and M5) was studied. The use of 160 mg/L Fe3O4 nanoparticles in combination with mixtures of different ratios (M4, M5, and M6) was further investigated. The findings indicate that the addition of Fe3O4 nanoparticles at a concentration of 40 mg/L to anaerobic batches did not significantly impact the hydrolysis process and subsequent methane production. Exposing the samples to Fe3O4 NPs at concentrations of 80, 120, and 160 mg/L resulted in a similar positive effect, as evidenced by hydrolysis percentages of approximately 94%, compared to 60% for the control (C2). Furthermore, methane production also increased. The use of Fe3O4 nanoparticles at a concentration of 160 mg/L resulted in biodegradability of 97.3%, compared to 51.4% for the control incubation (C2). Moreover, the findings demonstrate that supplementing anaerobic batches with 160 mg/L Fe3O4 NPs at varying mixture ratios (M4, M5, and M6) had a significant impact on both hydrolysis and methane production. Specifically, hydrolysis percentages of 94.24, 98.74, and 96.78% were achieved for M4, M5, and M6, respectively, whereas the percentages for the control incubation (C1, C2, and C3) were only 56.78, 60.21, and 58.74%. Additionally, the use of 160 mg/L Fe3O4 NPs in mixtures M4, M5, and M6 resulted in biodegradability percentages of 78.4, 97.3, and 88.3%, respectively. In contrast, for the control incubation (C1, C2, and C3) biodegradability was only 44.24, 51.4, and 49.1%.

Impact of Iron Oxide Nanoparticles on the Growth, Vermicomposting Efficiency and Nutritional Status of Vermicompost through Eisenia fetida

Impact of Iron Oxide Nanoparticles on the Growth, Vermicomposting Efficiency and Nutritional Status of Vermicompost through Eisenia fetida

Impact of iron oxide nanoparticles on a lead-polluted water–soil–plant system under alternating periods of water stress

Iron oxide nanoparticles (IONPs) are promising materials for the remediation of trace elements, which are a significant source of soil pollution.

Impact of Iron oxide nanoparticles on sustainable production of biogas through anaerobic co-digestion of chicken waste and wastewater

As The effect of iron oxide nanoparticles (IONPs) on the anaerobic co-digestion (AD) of olive mill wastewater and chicken manure was investigated. In mesophilic conditions, biogas yield, methane (CH4) content, the removal efficiency of TS, VS., acidification and hydrolysis percentage, and contaminant removal efficiency were investigated. Supplementing AD with IONPs at a concentration of 20 mg/g VS. > IONPs and INOPs >30 mg/g VS. causes an inhibitor impact on biogas, methane generation, and hydrolysis. Furthermore, implantation with 20–30 mg of IONPs/kg VS. has induced an equivalent favorable impact, with hydrolysis percentages reaching roughly 7.2%–15.1% compared to the control test, in addition to a 1.3%–4.2% enhancement in methane generation yield. The maximum acidification concentration after five days of the incubation of 1,084, 9,463, and 760 g/L was attained with IONPs dosages of 25, 30, and 20 mg/g VS., respectively, compared to 713 g/L obtained with the control test. The results have illustrated that supplementing AD with a specific concentration of IONPs (20–30 mg/g VS.) has a significant effect and enhances the inhibitor removal efficiency, most possibly due to the small surface area of IONP particles. The resultant increase in the active surface area enhances the enzyme diffusion within the substrate. This study provides new data specifying the enhancement of iron oxide nanoparticles (IONPs) and identifies the impact of IONP doses at various concentrations on the AD of olive mill wastewater and chicken waste.

Evaluating the impact of Iron Oxide nanoparticles (IO-NPs) and IO-NPs doped granular activated carbon on the anaerobic digestion of food waste at mesophilic and thermophilic temperature

The impact of Iron oxide nanoparticles (IO-NPs) and IO-NPs doped granular activated carbon (GAC) at three concentration levels of 0.16 g/L, 0.32 g/L and 0.48 g/L for IO-NPs and 2 g/L, 4 g/L and 6 g/L for IO-NPs doped GAC on the anaerobic digestion (AD) of food waste (FW) at mesophilic (35 ± 2 oC) and thermophilic (55 ± 2 oC) temperature has been investigated. Results revealed that the % utilization of volatile fatty acids (VFAs) ranged between 83% and 92% in mesophilic reactors (MRs) while it was between 79% and 84% in thermophilic (TRs) indicating elevated temperatures may lead to VFAs accumulation in the long term operation. Results disclosed that the CH4 (%) and yield was 60% and 0.25 L CH4/g VSreduced in MR-Control and 62% and 0.29 L CH4/g VSreduced in TR-Control respectively. Similarly, in the reactors with 0.32 g/L of IO-NPs the maximum CH4 of 72% and 77% with an yield of 0.43 and 0.54 L CH4/g VSreduced was achieved at mesophilic and thermophilic temperature. Likewise using 4 g/L of IO NPs doped GAC, the maximum CH4 of 74% and 78%, with an yield of 0.41 and 0.53 L CH4/g VSreduced was achieved. This could be attributed to the syntrophic balance between the bacterial groups (Firmicutes, Bacteroidetes, Chloroflexi, Thermotogae) and archaeal groups (Methanosarcina, Methanothrix and Methanosaeta) that enhanced the electron transfer between cell to cell resulting in improved performance.

Antitumor impact of iron oxide nanoparticles in Ehrlich carcinoma-bearing mice

ABSTRACT Magnetic nanoparticles are promising approaches for cancer treatment. Iron oxide nanoparticles have been pre-formed by the co-precipitation method to investigate their antitumor activity on Ehrlich carcinoma in mice. The nanoparticles have been studied by UV spectrophotometer, transmission electron microscopy, dynamic light scattering, and zeta potential measurement. To determine the therapeutic impact of Iron oxide nanoparticles in tumor-bearing mice, in vivo experiments were carried out. Fourier Transform Infra-red (FTIR) spectroscopy and DNA comet assay were used to estimate the toxicity of nanoparticles of iron oxide. The tissue was analyzed using FTIR spectral parameters such as the area beneath the peak, peak position, and peak intensity. The results showed that iron oxide nanoparticles significantly led to antitumor activity against Ehrlich carcinoma. There was a low shift in position and intensity at the amide I band (1650 cm−1) in tissues of mice treated with iron oxide nanoparticles compared to the control group. Comet examination showed a decrease across all the parameters of the assay (tail moment, tail DNA percentage, and tail length) in the tumor + iron nanoparticles group, having values lower than the tumor group. According to the presented results, Iron oxide nanoparticles have exhibited therapeutic efficacy against cancer.

Anaerobic digestion of cabbage and cauliflower biowaste: Impact of iron oxide nanoparticles (IONPs) on biomethane and microbial communities alteration

Recently, vegetable wastes (VWs) utilization received much attention in anaerobic digestion for biomethane production due to its high availability. However, still efforts are needed to improve the VWs digestibility and understand the microbial communities involved in VWs degradation. In this study, iron oxide nanoparticles (IONPs) were synthesized and used in different dosages to systematically evaluate its effect on the digestion of cabbage (CB) and cauliflower leaves (CL). The morphology and particle size of synthesized IONPs were characterized by UV-VIS spectroscopy, FTIR, SEM/EDX, TEM, and XRD. The biomethane production was enhanced by 123% and 79% for CB at 40 mg/L and 60 mg/L IONPs while it was 138% and 106% for CL, respectively. Syntrophomonas, Clostridium, and Methanosarcina were significantly enhanced to 143%, 124%, and 81.55% upon the addition of 40 mg/L IONPs, respectively. Adding IONPs to VWs digestion offers a potential approach to promote key microbial communities and boost biomethane.

Elucidating the physiological mechanisms underlying enhanced arsenic hyperaccumulation by glutathione modified superparamagnetic iron oxide nanoparticles in Isatis cappadocica

Widespread arsenic (As) contamination is a severe environmental and public health concern. Isatis cappadocica, an arsenic hyperaccumulator, holds great potential to clean up As-contaminated soil and groundwater. Iron oxide is one of the most common metal oxides in the natural environment and its nanoparticulate form has been previously utilized for the removal of heavy metals/metalloids from wastewater. However, there is a paucity of information on the impact of iron oxide nanoparticles on the growth and physiological properties of I. cappadocica and its effectiveness on As removal. Current study reports for the first time the impact of superparamagnetic iron oxide nanoparticles and glutathione (GSH) modified superparamagnetic iron oxide nanoparticles (nFe3O4 and nFe3O4@GSH) on the physiological characteristic of I. cappadocica and its accumulation of As under hydroponic condition. nFe3O4@GSH alleviated the harmful impact of As and significantly increased the shoot biomass of I. cappadocica by enhancing the plant defense mechanisms. The application of GSH, nFe3O4 and nFe3O4@GSH all lowered the As concentration in plant shoots as a protective mechanism. However, the substantial shoot biomass increase due to nFe3O4@GSH resulted in a 56% higher As accumulation in plant shoots than in plants exposed to As alone, indicating the strong effectiveness of nFe3O4@GSH as a novel enhancer of the As phytoremediation by I. cappadocica. Our data further showed that the beneficial effect of nFe3O4@GSH on As phytoremediation is due to the enhancement of activities of several enzymatic and nonenzymatic antioxidants.

Impact of iron oxide nanoparticles on xenobiotic metabolism in HepaRG cells

Iron oxide nanoparticles are used in various industrial fields, as a tool in biomedicine as well as in food colorants, and can therefore reach human metabolism via oral uptake or injection. However, their effects on the human body, especially the liver as one of the first target organs is still under elucidation. Here, we studied the influence of different representative iron oxide materials on xenobiotic metabolism of HepaRG cells. These included four iron oxide nanoparticles, one commercially available yellow food pigment (E172), and non-particulate ionic control FeSO4. The nanoparticles had different chemical and crystalline structures and differed in size and shape and were used at a concentration of 50µg Fe/mL. We found that various CYP enzymes were downregulated by some but not all iron oxide nanoparticles, with the Fe3O4-particle, both γ-Fe2O3-particles, and FeSO4 exhibiting the strongest effects, the yellow food pigment E172 showing a minor effect and an α-Fe2O3 nanoparticle leading to almost no inhibition of phase I machinery. The downregulation was seen at the mRNA, protein expression, and activity levels. Thereby, no dependency on the size or chemical structure was found. This underlines the difficulty of the grouping of nanomaterials regarding their physiological impact, suggesting that every iron oxide nanoparticle species needs to be evaluated in a case-by-case approach.

Coating iron oxide nanoparticles with mesoporous silica reduces their interaction and impact on S. oneidensis MR-1

Here, we investigate the impact of iron oxide nanoparticles (IONPs) and mesoporous silica-coated iron oxide nanoparticles (msIONPs) on Shewanella oneidensis in an aerobic environment, which is likely the main environment where such nanoparticles will end up after use in consumer products or biomedical applications. Monitoring the viability of S. oneidensis, a model environmental organism, after exposure to the nanoparticles reveals that IONPs promote bacterial survival, while msIONPs do not impact survival. These apparent impacts are correlated with association of the nanoparticles with the bacterial membrane, as revealed by TEM and ICP-MS studies, and upregulation of membrane-associated genes. However, similar survival in bacteria was observed when exposed to equivalent concentrations of released ions from each nanomaterial, indicating that aqueous nanoparticle transformations are responsible for the observed changes in bacterial viability. Therefore, this work demonstrates that a simple mesoporous silica coating can control the dissolution of the IONP core by greatly reducing the amount of released iron ions, making msIONPs a more sustainable option to reduce perturbations to the ecosystem upon release of nanoparticles into the environment.

Effect of Iron Oxide Nanoparticles on the Oxidation and Secondary Structure of Growth Hormone

Oxidation of therapeutic proteins (TPs) can lead to changes in their pharmacokinetics, biological activity and immunogenicity. Metal impurities such as iron are known to increase oxidation of TPs, but nanoparticulate metals have unique physical and chemical properties compared to the bulk material or free metal ions. Iron oxide nanoparticles (IONPs) may originate from equipment used in the manufacturing of TPs or from needles during injection. In this study, the impact of IONPs on oxidation of a model protein, rat growth hormone (rGH), was investigated under chemical stress. Hydrogen peroxide (H2O2)- and 2,2′-azobis (2-methylpropionamidine) dihydrochloride oxidized methionine residues of rGH, but unexpectedly, oxidation was suppressed in the presence of IONPs compared to a phosphate buffer control. Fourier transform infrared spectroscopy indicated splitting of the α-helical absorbance band in the presence of IONPs, whereas circular dichroism spectra showed a reduced α-helical contribution with increasing temperature for both rGH and rGH-IONP mixtures. The results collectively indicate that IONPs can increase the chemical stability of rGH by altering the kinetics and preference of amino acid residues that are oxidized, although the changes in protein secondary structure by IONPs may lead to alterations of physical stability.

Toxicological assessment of silica-coated iron oxide nanoparticles in human astrocytes

Iron oxide nanoparticles (ION) have great potential for an increasing number of medical and biological applications, particularly those focused on nervous system. Although ION seem to be biocompatible and present low toxicity, it is imperative to unveil the potential risk for the nervous system associated to their exposure, especially because current data on ION effects on human nervous cells are scarce. Thus, in the present study potential toxicity associated with silica-coated ION (S-ION) exposure was evaluated on human A172 glioblastoma cells. To this aim, a complete toxicological screening testing several exposure times (3 and 24 h), nanoparticle concentrations (5–100 μg/ml), and culture media (complete and serum-free) was performed to firstly assess S-ION effects at different levels, including cytotoxicity – lactate dehydrogenase assay, analysis of cell cycle and cell death production – and genotoxicity – H2AX phosphorylation assessment, comet assay, micronucleus test and DNA repair competence assay. Results obtained showed that S-ION exhibit certain cytotoxicity, especially in serum-free medium, related to cell cycle disruption and cell death induction. However, scarce genotoxic effects and no alteration of the DNA repair process were observed. Results obtained in this work contribute to increase the knowledge on the impact of ION on the human nervous system cells.

Insights into the toxicity of iron oxides nanoparticles in land snails

The use of manufactured nanoparticles (NPs) is spreading rapidly across technology and medicine fields, posing concerns about their consequence on ecosystems and human health. The present study aims to assess the biological responses triggered by iron oxide NPs (IONPs) and iron oxide NPs incorporated into zeolite (IONPZ) in relation to oxidative stress on the land snail Helix aspersa in order to investigate its use as a biomarker for terrestrial environments. Morphology and structure of both NPs were characterized. Snail food was supplemented with a range of concentrations of IONPs and IONPZ and values of the hemocyte lysosomal membranes' destabilization by 50% were estimated by the neutral red retention (NRRT50) assay. Subsequently, snails were fed with NPs concentrations equal to half of the NRRT50 values, 0.05 mg L−1 for IONPs and 1 mg L−1 for IONPZ, for 1, 5, 10 and 20 days. Both effectors induced oxidative stress in snails' hemocytes compared to untreated animals. The latter was detected by NRRT changes, reactive oxygen species (ROS) production, lipid peroxidation estimation, DNA integrity loss, measurement of protein carbonyl content by an enzyme-linked immunoabsorbent assay (ELISA), determination of ubiquitin conjugates and cleaved caspases conjugates levels. The results showed that the simultaneous use of the parameters tested could constitute possible reliable biomarkers for the evaluation of NPs toxicity. However, more research is required in order to enlighten the disposal and toxic impact of iron oxide NPs on the environment to ensure their safe use in the future.

Application of Iron Oxide Nanoparticles in Contemporary Experimental Physiology and Cell Biology Research

Abstract Recent findings have suggested that iron oxide nanoparticles (IONPs) have some exceptional chemical characteristics which make them useful in both experimental physiology and cell biology research. These nanoparticles might be applied as drug delivery systems for anti-cancer and other medications. Also, IONPs might be a valuable part of many novel bioassays in various fundamental medical fields. In recent years, several studies have indicated that IONPs may have certain cytotoxic and genotoxic potential in living systems. During in vitro conditions, IONPs might induce generation of reactive oxygen species and cause oxidative stress in some cell populations. The toxicity of IONPs is not yet fully understood, and additional research is needed to confirm if IONPs have detrimental effects on human health. This short article focuses on the recent developments and trends in the fields of nanomedicine and nanobiology regarding iron oxide nanomaterials and their application in fundamental medical disciplines such as experimental physiology. We discuss our previously published works on structural effects of IONPs and other metallic nanoparticles on cell nucleus in in vitro conditions. We also describe our recent findings regarding the impact of IONPs on certain biophysical properties of subcellular components.

Microbial stress mediated intercellular nanotubes in an anaerobic microbial consortium digesting cellulose

The anaerobic digestion process is a multi - step reaction dependent on concerted activities such as exchange of metabolites among physiologically different microbial communities. This study investigated the impact of iron oxide nanoparticles on the anaerobic sludge microbiota. It was shown there were three distinct microbial phases following addition of the nanoparticles: microbial stress and cell death of approximately one log order of magnitude, followed by microbial rewiring, and recovery. Furthermore, it was noted that cellular stress led to the establishment of intercellular nanotubes within the microbial biomass. Intercellular nanotube - mediated communication among genetically engineered microorganisms and ad hoc assembled co - cultures have been previously reported. This study presents evidence of intercellular nanotube formation within an environmental sample – i.e., anaerobic sludge microbiota subjected to stress. Our observations suggested a mode of microbial communication in the anaerobic digestion process not previously explored and which may have implications on bioreactor design and microbial functions.

Effects of iron nanoparticles on Mentha piperita L. under salinity stress

The progress of nanotechnology presents many nanoparticles that are important in medicine, agriculture and industry. Quickly and entirely absorbed by plants, nano-compounds and remedy their nutrient deficiency and satisfy this need. Iron oxide nanoparticles with suitable surface chemistry can be used as a rich source of iron for plants due to its gradual release of Fe in a wide pH range (pH 3 to 11). The present study investigated the impact of iron oxide nanoparticles (Fe2O3NPs in 0, 10, 20 and 30 µM concentrations) on physiological parameters of peppermint (Mentha piperita) under salt stress (0, 50, 100 and 150 mM concentrations of NaCl). Fe2O3NPs caused increases in leaf fresh weight and dry weight, phosphorus, potassium, iron, zinc, and calcium contents of the peppermint under salinity stress but did not have an effect on the sodium element. 30 µM concentration of Fe2O3NP was more impressive. Lipid peroxidation and proline contents of the peppermint under salinity decreased significantly by applying Fe2O3NPs. The maximum activities of total antioxidant enzymes (I %), catalase, superoxide dismutase, and guailcol peroxidase were observed in plants treated with 150 mM of NaCl, but application of Fe2O3NPs declined these antioxidant activities. The results suggest that the appropriate concentration of iron nanoparticles could be used for stress resistance of the peppermint.