Antitumor impact of iron oxide nanoparticles in Ehrlich carcinoma-bearing mice

Abstract

ABSTRACT Magnetic nanoparticles are promising approaches for cancer treatment. Iron oxide nanoparticles have been pre-formed by the co-precipitation method to investigate their antitumor activity on Ehrlich carcinoma in mice. The nanoparticles have been studied by UV spectrophotometer, transmission electron microscopy, dynamic light scattering, and zeta potential measurement. To determine the therapeutic impact of Iron oxide nanoparticles in tumor-bearing mice, in vivo experiments were carried out. Fourier Transform Infra-red (FTIR) spectroscopy and DNA comet assay were used to estimate the toxicity of nanoparticles of iron oxide. The tissue was analyzed using FTIR spectral parameters such as the area beneath the peak, peak position, and peak intensity. The results showed that iron oxide nanoparticles significantly led to antitumor activity against Ehrlich carcinoma. There was a low shift in position and intensity at the amide I band (1650 cm−1) in tissues of mice treated with iron oxide nanoparticles compared to the control group. Comet examination showed a decrease across all the parameters of the assay (tail moment, tail DNA percentage, and tail length) in the tumor + iron nanoparticles group, having values lower than the tumor group. According to the presented results, Iron oxide nanoparticles have exhibited therapeutic efficacy against cancer.