Immunotropic Effects Research Articles

A novel immunopharmacological biocompound

The publication is devoted to topical issues of experimental study of new biocompounds, based on the creation of a consortium of biological composite compounds – biological active substances (metabiotics) produced by strains 59T and 60T of saprophytic compounds of the genus Bacillus subtilis. These strains are very promising for the creation of hepatopicts, new medicinal substances, in the creation and development of a new pharmacological class of hepatoprotectors. Previous studies have shown the safety and hepatoprotective effect of biologically active drugs. It is worth noting that the very effective compounds are the produced biologically active substances – metabolites of bacterial origin, on the basis of which it seems appropriate to create a new class of drugs – metabiotics. The absence of vegetative probiotic cells in such compounds will reduce the additional immune load on the body. The combined use of these compounds provides a potentiated pharmacological effect. In this regard, it seemed appropriate, under the conditions of modeling toxic liver damage by carbon tetrachloride, to conduct an experimental assessment of the immunotropic effect of biologically active substances (BAS) on laboratory animals in order to confirm the effect on cellular factors of immunity. The purpose of the study was to study the effect of the combined use of dietary supplements produced by Bacillus subtilis microorganisms on indicators of cellular immunity in laboratory animals with toxic liver damage. Acute toxic hepatitis was reproduced in white laboratory rats with repeated intragastric administration of carbon tetrachloride. The comparison drug was a registered hepatoprotective drug – ursosan. The immunotropic effect was assessed using factors such as: phagocytic activity of macrophages and neutrophils (FA), NBT test, quantitative assessment of antibody-forming cells, T and B lymphocytes. As a result of the studies performed, reliable data were obtained on an increase in the number of T and B lymphocytes, antibody-forming cells, as well as an increase in FA, which confirms the activation of all parts of cellular immunity in conditions of acute toxic liver damage. The data obtained allow us to recommend the studied biocompound for the creation of new drug candidates of microbiological origin, hepatoprotective agents with immunotropic effects.

Immunomodulatory activity of red algal galactans and their partially depolymerized derivatives in RAW264.7 macrophages

Funoran and furcellaran were isolated through a successive cascade extraction process, followed by the depolymerization of extracted polymers via an auto-hydrolysis process. The molecular weight and structural peculiarities of both native and partially depolymerized polysaccharides were investigated using size-exclusion chromatography (SEC), FTIR, and NMR spectroscopy. Immunotropic effects of the native and partially depolymerized polysaccharides were explored through various in vitro assays. Although both higher and lower molecular weight funoran exhibited anti-inflammatory activity on LPS-stimulated RAW264.7 cells by significantly downregulating iNOS and COX-2 gene expression, as well as the secretion of pro-inflammatory cytokines, native funoran performed slightly better. Conversely, higher molecular weight furcellaran remarkably activated RAW264.7 cells compared to the non-treated control by inducing inflammatory mediators and pro-inflammatory cytokines, including the anti-inflammatory cytokine IL-10. Lower molecular weight furcellaran was unable to activate the macrophages, showing a similar behavior pattern to funoran samples in LPS-treated cells. Furthermore, the TLR4/NF-κB signaling pathway appears to be modulated by sulfated polysaccharides, leading to both anti-inflammatory and immunostimulatory responses in RAW264.7 cells through blocking and activating mechanisms. These findings indicate that sulfated polysaccharides could be promising therapeutic agents, and indeed, the molecular weight of polysaccharides plays a crucial role in the immune response of RAW264.7 macrophages.

On the immunotropic effects of a new combined pharmaconutraceutical preparation in osteoarthritis

On the immunotropic effects of a new combined pharmaconutraceutical preparation in osteoarthritis

Multifactorial effects of <i>Lactobacillus paracasei DG</i> in the regulation of the microbial-tissue complex: A review

The human gut microbiome is a complex biological system whose functions and metabolic processes are the result of multiple interactions between microbial groups. Within these groups and between them, specific relationships are formed that allow duplicating and reserving individual functions, systematically managing their implementation and, in general, ensuring the reliable functioning of the entire microbiome, including in the interests of the host macroorganism. The functional and metabolic microbial nucleus plays a crucial role in the functioning of microbiocenosis as a whole system. Due to its natural properties, the modern probiotic Lactobacillus paracasei DG performs the function of a conductor of the microbiocenosis of the gastrointestinal tract, regulating the metabolic activity of the intestinal normoflora, realizing immunotropic effects due to the production of exopolysaccharides, including previously unknown exopolysaccharides b, maintaining the integrity of the intestinal epithelial barrier, exerting antiviral action, exerting distant effects against microbiocenoses of other ecological niches.

In Vitro Study of the Cytotoxic and Immunotropic Effects of Molnupiravir and Favipiravir

In Vitro Study of the Cytotoxic and Immunotropic Effects of Molnupiravir and Favipiravir

Galectin-3 as a modulator of cytokine-mediated lymphocyte cooperation in vitro

Aim of the study. To investigate the effect of recombinant galectin-3 on the cytokines secretion of various subpopulations of helper-lymphocytes (Th1, Th2, Th17, Treg) in culture in vitro. Material and methods. The material for the study was peripheral blood from healthy people (n=17), from which lymphocytes were isolated by gradient centrifugation. Lymphocytes were cultured for 72 hours in a CO2-incubator with recombinant galectin-3 and activating antibodies (antiCD3/antiCD28). The concentration of cytokines (IL-10, IL-13, IL-17A, IL-22, IFN, TNF, TGF1) in the supernatants of lymphocyte cultures was determined by enzyme immunoassay. Results. Recombinant galectin-3 in vitro enhanced the secretion of IL-17A, IL-22 by lymphocytes, acting at a dose of 0.5 g/ml; IL-13, TNF and IFN at doses of 0.5 g/ml and 1 /ml (more pronounced when acting at a dose of 0.5 g/ml) and inhibited the production of IL-17, IL-22 at a dose of 1 g/ml and IL-10, TGF1 when testing both concentrations. Conclusion: Galectin-3 has a dose-dependent modulating effect on the cytokine-producing function of healthy donors lymphocytes in vitro. Functional imbalance in blood lymphocytes under the action of recombinant galectin-3 is manifested by induction of pro-inflammatory (IFN, IL-17, IL-22, TNF) and anti-inflammatory (interleukin-13) cytokines secretion, against the background of oppression of the suppressor cytokines (IL-10 and TGF1) production. A detailed study of the immunotropic effects of galectin-3 in relation to individual lymphocytes subpopulations is relevant from the view point of development new approaches to the treatment of tumor and autoimmune diseases accompanied by excessive production of this lectin.

Pharmacological body-wide and immunotropic effects of galavit in the treatment of patients with purulent-inflammatory diseases of various origins and allergization

Introduction: Patients with purulent-inflammatory diseases (PID), especially those with allergization, manifest chronicity, low treatment efficiency, and the risk of relapses; the above actualizes the search for innovative treatment technologies.
 The aim of the study: The aim of the study was to specify pharmacological (body-wide) and specific (immunotropic) effects of the immunomodulator galavit additionally prescribed in the context of basic treatment for patients with deep pyoderma, chronic pyelonephritis, adnexitis, purulent infection of soft tissues, complicated by atopic dermatitis.
 Materials and Methods: The study included 331 patients with PID and 30 healthy volunteers of the same age and gender who were examined prior and following the basic treatment of each nosoform. The diverse clinical and laboratory examination options were applied to investigate 17 clinical, 7 hematological, 16 immunological, 13 metabolic parameters that were grouped into clinical, hematological, immunological and metabolic syndromes, and divided into links – cellular, humoral, phagocytic, cytokine, metabolic and antioxidant. The results were statistically processed using variational parametric and nonparametric techniques; this allowed calculating the coefficient of diagnostic value, diagnostic formulas of disorders and targets of galavit under various conditions.
 Results and Discussion: Targeted pharmacological therapy with galavit reliably eliminates clinical, hemato-immuno-metabolic disorders in patients with PID, though dependently on the nosoform of the disease.
 Conclusion: It was demonstrated that the correction of clinical and laboratory disorders depend on PID pathogenesis and allergization; the laboratory mechanism of therapeutic effects was specified.

<i>In vitro</i> immunomodulatory effect of siRNA complexes in the influenza infection

About 1.2 billion cases of influenza infection with up to 5 million cases of severe disease and up to 650,000 deaths from influenza and its complications are registered annually worldwide. High rates of morbidity and mortality are attributed to immunomodulatory properties of some proteins produced by the influenza viruses. Among these proteins, NS-1 is the most studied. One of its main functions is to disrupt the functioning of interferon-mediated defense mechanisms of the body thus causing suppressed production of different components of humoral immunity, which leads to an insufficiency of the immune response. It is known that miRNAs directed to cellular genes, which are involved in the process of viral reproduction, showing a pronounced antiviral activity. At the same time, only few studies have been focused on evaluation of their immunotropic effects. Therefore, the aim of our study was to quantify the concentrations of IFNα, IFNγ, TNFα and IL-10 cytokines as a result of complex suppression of the cellular FLT4, Nup98 and Nup205 gene activity, whose expression products play an important role in the reproduction of the influenza virus.
 We have shown that the use of siRNA complexes also leads to an increase in the IFNα, IFNγ, TNFα and IL-10 concentrations. IL-10 production is absent on the first day after infection, but begins to increase on the second and third days. Moreover, in some cases, there is an increase in IFNα and IFNγ concentration on the first day after infection followed by decrease in their concentrations by the third day. This finding indicates that, upon supplement of the siRNA complexes, the cytokine profile is normalized under the influence of IL-10.

Immunopharmacological aspects of studying immunotropic properties of a novel biocompound

The article presents an experimental study of immunotropic activity of a new compound, a metabiotic, based on metabolites (biologically active substances) produced by a of saprophytic Bacillus subtilis B-9909 strain, being safe for health and standardized at Russian National Collection of Industrial Microorganisms (RNCIM). The aim of our study was to experimentally evaluate the immunotropic effects of metabolites produced by the probiotic Bacillus subtilis B-9909 upon the parameters of cellular immunity tested in the animal model of induced toxic liver damage. The metabolites were isolated from the cultural liquid of Bacillus subtilis culture (RNCIM strain B-9909) during its deep cultivation in a medium containing hydrochloric acid hydrolyzate of soy flour, or pancreatic hydrolyzate of casein. At 16-18 hours of cultivation, the cells were in the exponential growth phase. The indices of cellular status were studied in experimental groups of animals, when assessing therapeutic efficacy of an experimental metabiotic sample, as compared to a group of laboratory animals treated with the reference drug (ursosan). We assessed the quantitative indicators of blood as follows: determination of phagocytic activity (FA) of peripheral blood neutrophils; measurement of metabolic activity in peripheral blood neutrophils by means of NBT-test; quantitative determination of T and B lymphocytes; the number of antibody-forming cells (AFC). Liver damage was studied in a model of acute toxic hepatitis in albino rats. Experimental toxic hepatitis was induced by 40% solution of CCl4 in vaseline oil injected intragastrically for 2 weeks at the dose of 0.2 g/kg. The results of experimental studies showed activation of phagocytic activity by peripheral blood neutrophils at the early terms of experiment, being also confirmed by the results of NBT-testing. Moreover, at the early observation terms, a significantly increased representation of T and B lymphocyte populations as well as AFC numbers, were revealed thus suggesting activation of all components of cellular immunity in response to the carbon tetrachloride toxicity. Thus, the reported studies on the cellular status of laboratory animals treated with metabolites produced by probiotic microorganisms of the genus Bacillus subtilis (B-9909) on laboratory animals with toxic liver damage allow us to conclude that the test sample of this metabiotic preparation exerts a significant immunomodulatory effect, when compared with ursosan. This evidence allows us to consider this compound a promising candidate drug for a new hepatoprotector with immunotropic effect.

Immunomodulatory properties of plant polyphenols shown in an <i>in vitro</i> experimental model

Polyphenols exert a wide range of biological effects, including immunomodulatory action. Studying the effects of flavonoids on phagocytic activity of specialized phagocytic cells seems to be a rather promising direction for their further usage as pharmacological (therapeutic) agents. Quercetin and luteolin are the most commonly studied flavonoid substances with pleiotropic action. In-depth study and understanding of immunotropic mechanisms (e.g., regulation of phagocytosis) is a prerequisite for adequate pharmacotherapy in infectious conditions, nonspecific inflammatory diseases, autoimmune and oncological disorders. The aim of our work is to study the effect of flavonoids upon phagocytic activity of professional phagocytes (neutrophils) using an in vitro test system. The biological material (venous blood) from 30 practically healthy people (adults n = 15, children n = 15) was used in the present work. The study was carried out in accordance with established international regulations. 0.5 mg/L of Luteolin (basic substance content ≥ 98%) and Quercetin (basic substance content ≥ 95%) were added to experimental samples and incubated for 20 min at 37 °С. The percentage of phagocytosis, phagocytic number (mean number of formalin-treated sheep erythrocytes engulfed per one neutrophil) was determined in control and experimental samples using light microscopy. The unidirectional nature of phagocytosis inhibition by quercetin and luteolin was noted in the test experiments. A statistically significant (p < 0.05) decrease in the phagocytosis intensity by 10% was shown in experimental blood samples obtained from adult patients compared with control values. When quercetin and luteolin were added to blood samples obtained from children, a statistically significant (p < 0.05) decrease in phagocytosis by 30% was noted against control values. At the same time, the mean percentage of phagocytosis and phagocytic number in blood samples after the addition of flavonoids were found to be in the range of reference values, thus suggesting adequacy and physiological suppression of excessive activities of innate immunity compartments by quercetin and luteolin. At this concentration, the flavonoids were found to exert a more pronounced suppressive effect on phagocytic activity in children. Modeling of immune response using the phagocytosis indices assayed in experimental in vitro models with neutrophils from practically healthy adults and children enables us to expand the knowledge of mechanisms underlying the immunotropic effects of flavonoids (quercetin and luteolin), in order to correct immunopathological conditions.

Immunotropic effects of including a combination of hepatoprotectors in the complex therapy of chronic pancreatitis combined with chronic viral hepatitis C

Among significant etiological factors contributing to chronic pancreatitis (CP), the infectious factor plays a role in 56.88% of patients and the hepatobiliary factor in 44.50%, which has drawn attention to the particular importance of these factors. Hepatitis C virus (HCV) is the most common and important viral agent that causes or worsens CP. HCV is not only a disease of the liver but also a systemic (generalized) infection that involves other organs, in particular the pancreas and the immune system.
 The aim is to study the state of the cellular and humoral immune systems of patients with CP against the background of treated chronic HCV (CHCV) under the influence of the proposed complex treatment with the additional inclusion of hepatotrophic drugs heparizine (HZ) and metadoxine (MD).
 Materials and methods. We examined 80 patients with a combined course of CP and CHCV. According to the methods of treatment, the patients were divided into 3 groups: group 1 (20 patients with CP and CHCV) received the generally accepted complex of protocol treatment (PT) for three months; group 2 (25 patients with CP and CHCV) received PT with the additional inclusion of the hepatotrophic agent Heparizine PLC 1 capsule 3 times a day after meals for 3 months; and group 3 (35 patients with CP and CVHC with CAGE ≥2.0) received PT with the additional inclusion of the hepatotrophic agent Heparizine PLC (one capsule) 3 times a day after meals for 3 months with the addition of MD (Liveria IS) 500 mg (one tablet) 2 times a day 15–30 min before meals for 3 months.
 Results and discussion. With the additional administration of HZ, a higher dynamics of cellular immunity indices was reached in relation to those in the PT group. When using a combination of HZ and MD, even better results were obtained in the dynamics of the following indices: an increase in the total population of T-lymphocytes by 5.2%, 22.9%, and 57.7% in groups 1–3, respectively; NK cells — by 13.1%, 23.0%, and 39.4%; T-helpers — by 10.7%, 22.8%, and 36.3%; T-suppressors — by 26.0%, 38.9%, and 45.3%; a decrease in the number of B-lymphocytes — by 10.4%, 18.9%, and 35.6%. An increase in the immunoregulatory index (CD4/CD8) was also achieved — by 11.5%, 17.7%, and 45.7%. The decrease in the levels of B-lymphocytes under the influence of treatment in all three groups was accompanied by a decrease in the activation of humoral factors in groups 1–3, respectively: a decrease in the content of IgG in blood serum by 8.7%, 15.5%, and 19.2%; IgA — by 21.8%, 70.2%, and 88.5%; and IgM — by 24.0%, 55.5%, and 84.9%; as well as a significant decrease in the levels of circulating immune complexes by 38.6%, 1.9 times and 2.9 times (p<0.001).
 Conclusion. A positive immunocorrective effect of all three programs of complex treatment was proven, with a significantly higher level of those with the additional inclusion of HZ and especially the combination of HZ and MD in the complex therapy of patients with CP against the background of CHCV treated causally. Based on the results of correcting cellular and humoral immunity as an integral part of nutritional trophological status, it seems reasonable to include the proposed programs to eliminate nutritional and other disorders in people with CP and CHCV.

Immunotropic effects of hypoglycemic agents on coronavirus infection: a view from the perspective of pharmacogenetics

Diabetes mellitus is a predictor of the severe course of a new coronavirus infection and high mortality, in this regard, the selection of appropriate hypoglycemic therapy is a vital issue. In this article, we paid attention to dipeptidyl peptidase-4 (DPP-4) inhibitors, since this group of drugs has features like low risk of hyperglycemia and immuno-mediated effects. For the most effective treatment, it is necessary to take into account the individual characteristics of each patient, namely, the achievements of pharmacogenetics. The genetic variability of the response to therapy with DPP-4 inhibitors is due to a variety of polymorphisms, several main variations are considered in the review. The ambiguity of the available studies on the effectiveness of DPP-4 inhibitors in patients with type 2 diabetes with COVID-19 indicates the need to continue pharmacogenetic studies. The combination of knowledge about the subtleties of the mechanisms of pharmacological action of drugs and individual characteristics of pharmacodinamics will ensure the greatest effectiveness and safety of personalized therapy of diabetes mellitus against the background of coronavirus infection.

STUDYING THE OSMOTIC ACTIVITY OF A POLYCOMPONENT REPARATIVE GEL

Abstract. Hydrogel-based wound dressings are currently in demand due to their positive effect on reparative processes. The range of such is quite diverse and is represented by gelling agents of both synthetic and natural origin. The latter are most often biopolymers, among which collagen deserves special attention. The most important properties of collagen are its biocompatibility, low level of immunogenicity, ability to biodegrade, which leads to the formation of degradation products that stimulate cell proliferation and the biosynthesis of native collagen. Collagen has optimal physical characteristics, which is important for ensuring the structural and mechanical properties of the compositions. In addition, collagen has been successfully used as a carrier for other pharmacologically active substances. In this regard, the expediency of developing a multicomponent gel with a predictable reparative effect based on collagen and a thick extract of maize columns with stigmas was shown. This medicinal plant raw material has anti-inflammatory, antioxidant, immunotropic effects, which suggests its positive effect on tissue healing processes. One of the important properties of the reparative gel is its osmotic activity, which determines the ability to absorb exudate and cleanse the surface of the wound from purulent secretions, reduce tissue hyperthermia and inflammatory edema. The purpose of this study is to study the osmotic activity of a multicomponent gel of reparative action based on collagen and a thick extract of maize columns with stigmas. The object of the study was experimental samples of a polycomponent gel. Osmotic activity was studied by the method of equilibrium dialysis according to Kruvchinsky. For the purpose of a comparative assessment of the osmotic activity of a multicomponent gel, a control study was carried out in parallel under the same conditions for a 0.9% sodium chloride solution. The results obtained demonstrated the presence of hyperosmolar characteristics in the experimental samples of the polycomponent gel during the first four hours of dialysis, which allows us to predict its positive effect on the processes of cleansing damaged tissues from pathological secretions and their effective repair.

Role of autonomous and endocrine factors in immunotropic effects of nitrogenous metabolites in patients with chronic pyelonephritis

Background. We have previously shown that nitrogenous metabolites have immunomodulatory effects in healthy rats and humans as well as patients with dysfunction of neuroendocrine-immune complex, encephalopatia and chronic pyelonephritis. The purpose of this study is clarification of the role of neuro-endocrine factors in their immunotropic activity in patients with chronic pyelonephritis. Materials and Methods. The object of observation were 17 men (aged 24-70 years) with chronic pyelonephritis in remission. The plasma levels and urinary excretion of nitrogenous metabolites as well as parameters of immunity and its neuro-endocrine regulation twice (on admission and after balneotherapy at the Truskavets’ Spa) was performed. Results. Judging by the multiple correlation coefficient, uricosuria exhibits maximal neuro-endocrine activity (R=0,780), followed by bilirubinemia (R=0,742), creatinineuria (R=0,692), uricemia (R=0,636), creatinineemia (R=0,632), urea excretion (R=0,536), instead urea plasma correlate with neuro-endocrine parameters insignificantly (R=0,360). Nitrogenous metabolites together determine the state of neuro-endocrine regulation by 94,2%, which, in turn, determine the state of immunity by 99,9%. Conclusion. Nitrogenous metabolites carry out immunomodulation in different ways: directly through aryl hydrocarbon (bilirubin), toll-like and adenosine (uric acid) receptors of immunocytes; through modulation of the activity of neurons of the autonomous nervous system and endocrinocytes with subsequent neuro-endocrine immunomodulation; and also, apparently, due to an off-receptor effect on neurons, endocrinocytes and immunocytes (urea and creatinine).

Role of autonomous and endocrine factors in immunotropic effects of nitrogenous metabolites in patients with chronic pyelonephritis

Role of autonomous and endocrine factors in immunotropic effects of nitrogenous metabolites in patients with chronic pyelonephritis

Study of the prebiotic and immunotropic effects of curcumin in animal models

One of the promising and dynamically developing areas of modern biology and medicine and pharmacy is the use of plant components as a source of biologically active compounds and their use for therapeutic and prophylactic purposes. The use of nutraceuticals and curcumin-based functional foods has gained popularity in the past few decades due to the increased interest in natural foods and their potential health benefits.

Similar and specific immunotropic effects of sulfate-chloride sodium-magnesium mineral waters "Myroslava" and "Khrystyna" of Truskavets' spa in healthy female rats

Similar and specific immunotropic effects of sulfate-chloride sodium-magnesium mineral waters "Myroslava" and "Khrystyna" of Truskavets' spa in healthy female rats

Similar and specific immunotropic effects of sulfate-chloride sodium-magnesium mineral waters "Myroslava" and "Khrystyna" of Truskavets’ spa in healthy female rats

Background. Earlier in an experiment on rats, we showed that newly created sulfate-chloride sodium-magnesium drinking mineral waters of Truskavets’ spa has a significant modulating effect on the parameters of metabolism and the autonomic nervous and endocrine systems. In this study, conducted in line with the concepts of neuroendocrine-immune complex and functional-metabolic continuum, data on the immunomodulatory effects of these waters on the same rats. Materials and Methods. Experiment was performed on 50 healthy female Wistar rats 230-290 g divided into 4 groups. Animals of the first group remained intact, using tap water from drinking ad libitum. Rats of the second (control) group for 6 days administered a single tap water through the tube at a dose of 1,5 mL/100 g of body mass. The rats of the main groups received the water "Myroslava" and "Khrystyna". The object of the study were the immune parameters of the thymus, spleen and blood. Results. The method of discriminant analysis revealed 12 parameters, according to which the animals loaded with mineral waters differed significantly from both control and intact animals. Classification accuracy is 86%. However, the difference between the immunotropic effects of mineral waters of different mineralization concerns only 9 parameters. Conclusion. The newly created sulfate-chloride sodium-magnesium drinking mineral waters of Truskavets’ spa have both similar and specific immunomodulating effects on healthy old female rats with weekly use. This provides a basis for preclinical studies.

Relationships between geomagnetic Ар-indeх and parameters of the immunity in patients with neuroendocrine-immune complex dysfunction in former sportsmen

Background. The effect of geomagnetism on human immunity has so far been studied through long-term observations. Recently, we have been detected the immediate immunotropic effects of the disturbances of the geomagnetic field (Ap-index) at multiple sclerosis patients. The aim of this study was to identify the immunotropic effects of geomagnetism on another contingent of people. Material and methods. The object of observation were 21 men (24-63 y) and 20 women (33-62 y) with neuroendocrine-immune complex dysfunction. Each patient was tested twice with an interval of 4 days. Observations were carried out on 09.06. and 13.06. 2015, 14.09 and 18.09. 2015, 27-28.03. and 04-05.04. 2018, 28.01. and 01.02. 2019. Retrospectively we recorded the geomagnetic Ap-Index on the day of testing and during the previous 7 days, using resource https://www.spaceweatherlive.com/. The content of subpopulations of lymphocytes expressing CD3, CD4, CD25, CD8, CD22 and CD56 receptors as well as the serum concentration of circulating immune complexes, immunoglobulins classes M, G, A, C-reactive protein and IL-1β was determined. The state of phagocytic function of neutrophils estimated by microbial count and phagocytic and killing indices against Staphylococcus aureus and Escherichia coli. Results. During the week, the average level of Ap-index ranged from 7 to 13 nT. Maximum coefficients of multiple correlation with immunity parameters were detected for Ap-index on the eve of blood sampling (R=0,768) and 5 days before it (R=0,758) while the minimum on 3 (R=0,541) and 2 (R=0,479) days before sampling. The canonical correlation between Ap-indices for 7 days before and on the day of testing, on the one hand, and the immunity parameters - on the other hand, was very strong: R=0,921; R2=0,849; χ2(200)=375; p<10-6. Conclusion. Disturbances of the geomagnetic field (Ap-index) has a significant immediate modulating effect on the immune parameters, mostly phagocytosis completeness, Igg A and M serum concentration, T-helper and B lymphocytes as well as eosinophils, rod-shaped neutrophils and monocytes blood level.

ISOLYQUIRITIGENIN AFFECTS PHAGOCYTES FUNCTIONS AND INCREASES MICE SURVIVAL RATE IN STAPHYLOCOCCAL INFECTION

The results of studying the effect of isoliquiritigenin on animal survival in the model of staphylococcal infection and the function of human and animal phagocytes are presented in this article.The aim of the investigation was to study the effect of an isoliquiritigenin preliminary administration on the survival of animals against the background of staphylococcal infection, as well as on the function of phagocytes in mice and humans.Materials and methods. To assess the survival of Balb/C mice, a model of infection caused by Staphylococcus aureus J49 ATCC 25923 with the construction of Kaplan-Meier curves, was used. The effect on the phagocytes functions was studied by assessing the peptone-induced migration of phagocytes into the abdominal cavity of Balb/C mice, the absorption activity of phagocytes (neutrophils and monocytes) of human blood, as well as their production of reactive oxygen intermediates (ROIs) using а flow cytometry.Results. It was found out that a preliminary triple intraperitoneal administration of isoliquiritigenin (30 mg/kg) increases the survival rate of Balb/C mice in staphylococcal infection caused by Staphylococcus aureus J49 ATCC 25923. At the same time, isoliquiritigenin dose-dependently activates the production of reactive oxygen intermediates by human neutrophils and monocytes without statistically significantly suppressing a phagocytic activity of monocytes and neutrophils against fluoresceinisothiocyanate-labeled S. aureus J 49 ATCC 25923, as well as peptone-induced migration of phagocytes into the abdominal cavity of mice.Conclusion. Thus, a preliminary administration of isoliquiritigenin increases the survival rate of mice with staphylococcal infection and increases the production of reactive oxygen intermediates by phagocytes. The data obtained, can become the basis for further research of antibacterial and immunotropic effects of isoliquiritigenin in order to find new drugs for the treatment of staphylococcal infection.