Immunohistochemical Expression Of E-cadherin Research Articles

Expression of E-Cadherin and N-Cadherin in the Endocervix as a Predictive Factor in Patients with Endometrial Cancer.

Endometrial cancer (EC) is the most common gynecological malignancy. This study aimed to evaluate the expression of E-cadherin and N-cadherin in primary endometrial lesions and the endocervix in patients with EC to identify noninvasive predictive factors. In this single-center retrospective study, data on 101 patients who underwent surgery for EC were collected. The immunohistochemical expression of E-cadherin and N-cadherin was assessed depending on the tumor grade, location, and cell differentiation. Correlations between E-cadherin and N-cadherin levels in the endocervix and the primary tumor were determined. The degree of histological tumor differentiation significantly affected E-cadherin expression (p = 0.04) but had no impact on N-cadherin levels. In type II EC, the expression of both cadherins in the tumor tissue differed from their endocervical levels. The expression of E-cadherin differed significantly between the endocervix (p < 0.001) and the tumor (p = 0.001), depending on the type of EC. The expression of E-cadherin was related to the N-cadherin level only in the endocervix in patients with type II EC (p = 0.02). E-cadherin and N-cadherin were expressed in the endocervix in patients with EC. The expression of cadherins, determined during cervical cytology, may be a valuable clinical marker of EC.

Exploring the Relationship between E-Cadherin and β-Catenin Cell Adhesion Proteins and Periacinar Retraction Clefting in Prostatic Adenocarcinoma.

Periacinar retraction clefts represent a histopathological criterion supporting the diagnosis of prostatic adenocarcinoma. The origin of these clefts in prostatic adenocarcinoma remains unclear. Exploring the established functions of E-cadherin and β-catenin as intercellular adhesion proteins, and aiming to elucidate the origin of periacinar retraction clefting, we conducted a correlation study between the immunohistochemical expression of E-cadherin and β-catenin and the presence of periacinar retraction clefts in prostatic adenocarcinoma. We examined 53 cases of morphologically diagnosed prostatic adenocarcinoma, assessing both the neoplastic and adjacent nonneoplastic prostatic tissues for the existence and degree of periacinar retraction clefts. Additionally, we analyzed the immunohistochemical expression of E-cadherin and β-catenin proteins in prostatic tissue and explored their correlation with periacinar retraction clefts, and Gleason score, Grade Group, preoperative serum prostate specific-antigen (sPSA) levels, surgical margin status, and Tumor, Node, Metastasis (TNM) stage in prostatic adenocarcinoma. Our study confirms that periacinar retraction clefting is significantly more extensive in prostatic adenocarcinoma than in nonneoplastic prostatic tissue (p < 0.001). We report a decreased expression of E-cadherin and β-catenin immunostaining in prostatic adenocarcinoma and a negative correlation with Gleason score and Grade Group. Periacinar retraction clefting positively correlated with E-cadherin and β-catenin ((rho = 0.350; p = 0.010) and (rho = 0.340; p = 0.012)) immunostaining in prostatic adenocarcinoma. Periacinar retraction clefts stand out as a dependable criterion in the diagnosis of prostatic adenocarcinoma. E-cadherin and β-catenin proteins are potential markers indicative of tumor progression and invasiveness in prostatic adenocarcinoma. Our discovery of a positive correlation between immunostaining of E-cadherin and β-catenin proteins and periacinar retraction clefts in prostatic adenocarcinoma aligns with the notion that periacinar retraction clefting is more characteristic of Gleason Grade3 pattern in prostatic adenocarcinomas, whereas the immunohistochemical expression of E-cadherin and β-catenin shows a decrease with increasing histopathological tumor grade.

Immunohistochemical assessment of the potential behavior of glandular odontogenic cyst and inflammatory periodontal cyst using E-cadherin and N-cadherin

BackgroundThis study is done to evaluate the immunohistochemical expression of E-cadherin and N-cadherin in glandular odontogenic cysts (GOC), inflammatory periodontal (IPDL) cysts, and dental follicles (DF), and if there is a correlation with their biological behavior GOC, IPDL cysts, and DF cases were collected and investigated by immunohistochemistry. The area percent of immunoexpressed markers was calculated by SPSS version 25.ResultsThe statistical analysis revealed a clear, statistically significant difference between the studied groups regarding the area percentage of the two immune-expression markers (p value = 0.000). The highest area percentage of the mean value of E-cadherin was recorded in IPDL cysts group with the most negligible value recorded in the group of GOC. On the other hand, the highest area percentage of the mean value of N-cadherin was documented in the group of GOC with the least recorded value was noted in the group of DF.ConclusionWe noted that the cadherin switch mechanism in the epithelial lining of odontogenic cysts is a critical step in the epithelial mesenchymal transition process which may associates with clinical behavior and may also impact the mode of treatment.

E-Cadherin Is Expressed in Epithelial Cells of the Choroid Plexus in Human and Mouse Brains.

Evidence showing the functional significance of the choroid plexus is accumulating. Epithelial cells with tight and adherens junctions of the choroid plexus play important roles in cerebrospinal fluid production and circadian rhythm formation. Although specific types of cadherin expressed in adherens junctions of choroid plexus epithelium (CPE) have been examined, they remained uncertain. Recent mass spectrometry and immunolocalization analysis revealed that non-epithelial cadherins, P- and N-cadherins, are expressed in the lateral membrane of CPE, whereas E-cadherin expression has not been confirmed in CPE of humans or mice. In this study, we examined E-cadherin expression in CPE of mice and humans by RT-PCR, immunohistochemical-, and Western blotting analyses. We confirmed, by using RT-PCR analysis, the mRNA expression of E-cadherin in the choroid plexus of mice. The immunohistochemical expression of E-cadherin was noted in the lateral membrane of CPE of mice and humans. We further confirmed, in Western blotting, the specific immunoreactivity for E-cadherin. Immunohistochemically, the expression of E- and N-cadherins or vimentin was unevenly distributed in some CPE, whereas that of E- and P-cadherins or β-catenin frequently co-existed in other CPE. These findings indicate that E-cadherin is expressed in the lateral membrane of CPE, possibly correlated with the expression of other cadherins and cytoplasmic proteins.

Immunohistochemical expression of E-cadherin and N-cadherin in endometrioid endometrial carcinoma and its precursor lesions

ObjectivesEndometrial carcinoma (EC), majority being endometroid endometrial carcinoma (EEC), is the most common invasive gynecological cancer world-wide. Epithelial mesenchymal transition (EMT) is the process that cells undergo to switch from a polarized epithelial phenotype to a motile mesenchymal phenotype. Loss of E-cadherin and expression of N-cadherin are a critical step for development and progression of malignant tumors. In present study we evaluated the immunohistochemical (IHC) expression of E-cadherin and N-cadherin with clinicopathological parameters of EEC and its precursors lesions. MethodsIt was a prospective nested case-control study involving women aged 35–70 years, whose endometrial biopsy were obtained for histological diagnosis. Signed informed consent was obtained before enrolling the women in the study, and the approval to conduct the study was obtained from the institutional ethics committee. The IHC was done to measure the E-cadherin and N-cadherin. ResultsReduced expression of E-cadherin was observed in 69% of cancer and 65% of precancer, however only 32% of normal proliferative endometrium showed reduced E-cadherin expression. N-cadherin expression was observed in 82% of EEC and 81% of precancer cases, while 11% of normal proliferative endometrium showed N-cadherin expression. Statistically significant downregulation of E-cadherin and upregulation of N-cadherin were observed in EEC and precancerous lesions as compared to normal control. ConclusionSince downregulation of E-cadherin was also associated with upregulation of N-cadherin, the integration of immuno-expression of both E-cadherin and N-cadherin might offer a potential therapeutic target for EEC.

Immunohistochemical expression of E-Cadherin and Cyclin D1 in different grades of oral squamous cell carcinoma.

Oral squamous cell carcinoma (OSCC) is the most common oral malignancy, representing up to 80-90% of all malignant neoplasms of the oral cavity. It results from the multistep accumulation of heterogeneous genetic changes. Important risk factors for OSCC include the use of tobacco or betel quid chewing, alcohol consumption, human papillomavirus and poor nutrition. E-Cadherin as a tumour suppressor gene sets a threshold for Wnt/β-catenin signalling. When expression of E-Cadherin is lost, potentiation of Wnt signalling pathway occurs leading to loss of cell-cell adhesion. The cyclin D1 gene (CCND1) located on chromosome 11q13 encodes a nuclear protein that is the regulatory subunit of Cdk-4 and Cdk-6. Cyclin D1 plays a major role in cell cycle transition from G1 to S phase by contributing to inactivation of the retinoblastoma gene product, and overexpression of CCND1 has been reported in 35-40% cases of OSCC. Considering this, we decided to evaluate and compare the expression of CE-Cadherin and Cyclin D1 in different grades of OSCC. A retrospective study was carried out on 60 formalin-fixed paraffin embedded tissue blocks comprising of 20 cases of well-differentiated OSCC, 20 cases of moderately differentiated OSCC and 20 cases of poorly differentiated OSCC. Diagnosed (using H and E), with oral mucosa taken as control. There was downregulation of E-Cadherin and overexpression of Cyclin D1 in increasing grades of OSCC and the difference was statistically significant. E-Cadherin was localised to membranous and shifted to cytoplasm as the grade worsened. Cyclin D1 was localised to nuclei of cells and the expression was seen more at the peripheral portions of tumour islands depicting the proliferative activity of tumour front. The study revealed a good prognostic role of both E-Cadherin and Cyclin D1 in OSCC. The markers can be used for prognostic as well as therapeutic purposes.

Immunohistochemical Expression of E-cadherin in Premalignant and Malignant Squamous Lesions of the Oral Cavity: A Cross-sectional Study

Introduction: Squamous Cell Carcinoma (SCC) constitutes 90% of the malignancies of the oral cavity, making it the 6th most common cancer worldwide. Premalignant lesions of the oral cavity carry an increased risk for the development of oral SCC, which is a multistage process. Numerous molecules are involved in this process, and E-cadherin is one of them. E-cadherin is a 120 kDa glycoprotein and a calcium-dependent molecule involved in cell adhesion. Altered expression of E-cadherin has been described in preinvasive lesions and malignancies of the cervix, oesophagus, and head and neck. Aim: The aim of this study was to observe the immunohistochemical (IHC) expression pattern of E-cadherin in premalignant and malignant lesions of the oral cavity and to correlate E-cadherin immunoexpression between different grades of dysplasia and malignancy. Materials and Methods: This cross-sectional, ambispective study was conducted at Sri Dharmasthala Manjunatheshwara College of Medical Sciences and Hospital, Dharwad, Karnataka, India. The study duration was two and a half years, with one year being retrospective from July 2014 to June 2015, and the remaining period being prospective from July 2015 to December 2016. Systematic random sampling was done to select 60 cases. Premalignant and malignant lesions of the oral squamous epithelium were classified according to the 2017 World Health Organization (WHO) classification and subjected to E-cadherin immunohistochemistry. Grading of expression was based on the percentage of cells showing continuous homogenous membranous staining: 0 (negative)=0 to 10%, 1+ (loss)=11-25%, 2+ (weak)=26% to 50%, 3+ (strong)=51% to 75%, 4+ (intense)=&gt;75% of cells. Results were analysed using the Statistical Package for Social Sciences version 20.0. Results: The expression of E-cadherin between the different grades of premalignant lesions varied significantly (p-value=0.003), ranging from intense expression in mild dysplasia to weak expression in high grades of dysplasia. Immunoexpression was intense (4+) in 50% of cases, strong (3+) in 39.28%, and weak (2+) in 7.14% of cases. The expression of E-cadherin between the different grades of malignant lesions also varied significantly (p-value=0.002), ranging from negative expression in poorly differentiated SCC to strong expression in well-differentiated SCC. Immunoexpression was negative (0) in 31.25% of cases, strong (3+) in 31.25%, weak (2+) in 15.62%, and (1+) in 21.87% of cases. The pattern of expression was also significant between premalignant and malignant lesions (p-value&lt;0.0001). Conclusion: As the grades of dysplasia and malignancy increase, the expression of E-cadherin reduces. Hence, there is a negative correlation between E-cadherin expression and the grades of dysplasia and malignancy.

Immunohistochemical Expression of E-Cadherin and β-Catenin in Oral Squamous Cell Carcinoma.

The E-cadherin/β-catenin protein complexes are actively involved in the epithelial-to-mesenchymal transition. Alterations in cadherin or catenin expression or function, play important roles in the development of invasive or metastatic phenotypes of cancers. The aim of this study was to assess the expression of E-cadherin and β-catenin in oral squamous cell carcinoma (OSCC) patients and to compare this with their clinico-pathological parameters. This was a cross-sectional study to assess the immunohistochemical expression of E-cadherin and β-catenin in 41 cases of OSCC. Data were analyzed using version 26 of SPSS software. Qualitative data were compared using chi-square statistics. Quantitative data were summarized using mean, standard deviation, and confidence interval and compared using a one-way analysis of variance test. The level of significance was set at P < 0.05. Overall, 95.1% of the cases had positive membrane expression for E-cadherin, while cytoplasmic staining was seen in 90.2% cases. Positive nuclear staining was seen in 46.3% cases. There was a decrease in the percentage of cytoplasmic and nuclear expression of E-cadherin as the OSCC became more poorly differentiated (χ2 = 13.96, P = 0.016). Also, a decrease in the percentage of nuclear expression of β-catenin in poorly differentiated cases was seen. However, no statistically significant difference was seen in the expression of β-catenin between the different histologic grades (χ2 = 4.8, P = 0.4). This study shows a reduction in the expression of E-cadherin and β-catenin as OSCC becomes less differentiated.

EMT and Tumor Turning Point Analysis in 3D Spheroid Culture of HNSCC and Mesenchymal Stem Cells.

The prognosis, metastasis, and behavior of head and neck squamous cancer cells are influenced by numerous factors concerning the tumor microenvironment, intercellular communication, and epithelial-to-mesenchymal transition (EMT). The aim of this study was to examine the codependent interaction of the mesenchymal stroma with head and neck squamous cell carcinoma (HNSCC) in a 3D spheroid structure. To simulate stroma-rich and -poor 3D tumor microenvironments, cells of the established cell SCC-040 were cultured with human mesenchymal stromal cells (MSCs), forming 3D stroma-tumor spheroids (STSs). STSs were compared to uniform spheroids of SCC-040 and MSC, respectively. The expressions of CD24, β-catenin, SNAI2, and ZEB2 were analyzed via RT-qPCR. The immunohistochemical expressions of E-cadherin, connexin 43, vimentin, and emmprin were analyzed, and protein expression pathways as well as Akt signaling were assessed via protein analysis. A promotive effect on the expressions of EMT markers ZEB2 (p = 0.0099), SNAI2 (p = 0.0352), and β-catenin (p = 0.0031) was demonstrated in STSs, as was the expression of Akt pathway proteins mTOR (p = 0.007), Erk1/2 (p = 0.0045), and p70 S6 Kinase (p = 0.0016). Our study demonstrated a change in genetic expression patterns early on in tumor development, indicating a tumor turning point.

Immunohistochemical expression of E-Cadherin in gastric carcinoma in Erbil city, Kurdistan Region

Background and objectives: Gastric carcinoma is at fourth position incidence-wise and holds a second place for deaths due to cancer. The Cadherins represent an important class of the adhesiveness molecules, which play an essential role in the homotypical cell-cell adhesion and in the complex process of invasion and metastasis. The point of this study is to identify the rate of the E-Cadherin immunoexpression in gastric carcinoma in Erbil locality and investigate its association with the clinico pathological parameters. Methods: A retrospective study was carried out in Rizgary teaching hospital and private laboratories in Erbil city for the period from Jan.2018 - Jan.2020. A total of 75 formalin fixed, paraffin embedded archival tissue blocks of total or partial gastrectomy samples for gastric carcinoma cases were collected, then E-cadherin immunohistochemistry was tested. Results: Twenty-one cases (28%) were categorized as high expression for E-Cadherin immunoexpression. At the same time 38 (50.7%) of the cases were classified as low expression and 16 (21.3%) of cases were labelled with no expression. E-Cadherin status was significantly associated with the tumor type and tumor grade, in which high E-Cadherin expression about (61.9%) was demonstrated in intestinal type tumors, (38.1%) in diffuse type tumors and about (57.1%) was performed in well-moderately differentiated tumors and (42.9%) in poorly differentiated tumors. While no significant association was found between E-Cadherin expression and other clinico. Conclusions: Our result support the hypothesis that the E-Cadherin immunostain is commonly expressed in Gastric cancer, and it was significantly related with type and grade of the tumor.

Immunohistochemical expression of E-cadherin, N-cadherin and Snail/slug in ameloblastoma

ContextAmeloblastoma is the most common odontogenic tumour and its histomorphological distinction into growth patterns and variants, does not accurately convey information about its biologic aggressiveness. Expression of epithelial mesenchymal transition (EMT) markers, which have been implicated in its etiogenesis, might assist in delineating aggressiveness across variants. This may help in formulating appropriate treatment modalities for its management AimsTo determine expression of SNAIL/SLUG and ECAD/NCAD in tumour cells in clinical and histological subtypes of ameloblastoma and to establish any association between the immunostaining profile and the biologic behaviour of histologic types of ameloblastoma. Settings/DesignThis is a retrospective study conducted to evaluate the immunoprofile of selected clinical subtypes and histological variants to EMT factors via immunostaining to SLUG and ECAD/NCAD antibodies. Mean aggregate scores for each antibody per variant was analyzed using ANOVA or Kruskal-Wallis test when appropriate. Agreement between AR and SR regions was correlated using Spearman's correlation co-efficient. ResultsA higher staining SLUG intensity in the stellate reticulum (SR) like areas relative to the ameloblast like areas (AR) was observed, without concomitant E-cadherin repression or elimination. However, a direct relationship between SLUG and N-cadherin was observed. ConclusionExpression of SLUG in the SR like areas can be utilized to predict the biologic behavior of specific clinico-histological variants, however its mechanism via alterations in cadherin switching is equivocal

Loss of E-Cadherin Expression Correlates With Ki-67 in Head and Neck Squamous Cell Carcinoma.

The aim of the study was to evaluate the correlation between the rate of proliferation and immunohistochemical expression of E-cadherin, and their predictive role in patients with head and neck squamous cell carcinoma (HNSCC). Samples were collected from 50 patients with HNSCC, and the expression of Ki-67 and E-cadherin was evaluated by immunohisto-chemistry (IHC). Previously, samples were conventionally stained with haematoxylin and eosin for histological diagnosis and grade. High E-cadherin expression was predominantly associated with less differentiated tumours (p<0.5; p=0.0305). Also, we observed a significant correlation between Ki-67 expression in tumour cells and tumour grade (p=0.0245). A strong correlation was noticed between low E-cadherin expression, increased Ki-67-proliferation rate and advanced T2-T3 tumour stage (p=0.0242). In this study we showed that Ki-67 proliferation rate and E-cadherin expression are important features in patients with HNSCC. Therefore, higher Ki-67 index values correlate with loss of E-cadherin expression, which indicates a poorer prognosis. These aspects support the use of both Ki-67 and E-cadherin as prognostic markers in specimens from patients with HNSCC.

The absence of early malignant changes in women subjected to Aquafilling breast augmentation on the basis of E-cadherin and N-cadherin immunohistochemical expression

The use of Aquafilling can be associated with a variety of health complications. The filler is an inflammatory process trigger at the site of tissue contact. The aim of this study was to semiquantitatively compare the immunohistochemical expression of E-cadherin and N-cadherin in tissue material from two groups of patients. The first group underwent surgical removal of Aquafilling from the breast, while the second was subjected to breast augmentation with implants or breast lifts (control group). The study group consisted of tissue samples from 16 patients who had Aquafilling removed, while the control group comprised samples from 16 patients who underwent breast augmentation with implants or breast lifts. Histopathology, immunohistochemistry and morphometric analyses were performed, taking into account the number of immunopositive cells and also the immunohistochemical reaction area for E-cadherin and N-cadherin. There were significantly more immunopositive N-cadherin cells in both groups. The immunohistochemical reaction area for N-cadherin did not differ between the two groups. However, the immunohistochemical reaction area for E-cadherin was significantly larger in the test group than in the control group. Moreover, the reaction area for N-cadherin was significantly smaller than that for E-cadherin. In the control group, no significant differences were detected between the immunohistochemical reaction area for N-cadherin and E-cadherin. Immunohistochemical evaluation of N-cadherin and E-cadherin tissue expression may be useful in assessing early cell junction changes. Furthermore, semiquantitative morphometric analysis allows these alterations to be more precisely determined.

Desmogleins 1, 3, and E-cadherin immunohistochemical expression within mucocutaneous pemphigus vulgaris.

Introductionpemphigus vulgaris (PV) is an autoimmune condition characterized by the loss of adhesion between the epithelial cells and blister formation. The production of autoantibodies against desmosomal proteins, namely, desmoglein (DSG) 1 and DSG3, is considered a main event of PV. A full understanding of the role of adhesion molecules in the pathogenesis of PV is not fully elucidated yet. This study aimed to evaluate and correlate the immunohistochemical expression of E-cadherin (E-cad), DSG1, and DSG3 proteins in oral and skin PV.Methodsthis study was a retrospective analysis study. Positive PV cases were stained with anti-E-cad, anti-DSG1, and anti-DSG3 antibodies. The expression of each marker was determined by two pathologists according to an established scoring system: (E-cad: negative, weak, moderate, and strong), (DSG1: negative, weak, and strong), and (DSG3: negative and positive). The Chi-square and Pearson´s correlation tests were used to statistically analyze the data.Resultsforty-three biopsies (26 skin and 17 oral tissue samples) from 22 males and 21 female PV patients were included. The median age was 40.50 years. In total, the immunohistochemical expression was negative for DSG3, E-cad, and DSG1 in 81.4%, 18.5%, and 16.4%, respectively. DSG1 expression was significantly higher in males than females. A statistically significant correlation was found between E-cad and DSG3 expressions.Conclusiona significant difference in the expression of markers of both oral and skin PV was absent. Downregulation of DSG3 expression was the hallmark feature that also showed a positive correlation with E-cad expression.

Evaluation of immunohistochemical expression of E-cadherin in pleomorphic adenoma and adenoid cystic carcinoma

Background:Pleomorphic adenoma (PA) and adenoid cystic carcinoma (ADCC) are benign and malignant salivary gland tumors, respectively, with distinct behavior. They have similar origins and cell components. E-cadherins are the main homophilic cell adhesion molecules, which play a central role in maintaining epithelial integrity, functioning in intercellular adhesion and differentiation. Hence, changes in E-cadherin function are reflected in the morphologic events associated with the cellular arrangement, movement and wound healing.Aim:To study and compare the expression of E-cadherin immunostaining in PA and ADCC.Materials and Methods:Fifteen cases of each PA and ADCC were immunohistochemically stained with E-cadherin. Five cases of normal salivary gland tissues were taken as the positive control.Mann–Whitney U-test was used for statistical analysis.Results:About 86.6% of PA cases showed homogeneous staining. 66.6% of cases of ADCC showed heterogeneous staining. PA, cribriform and tubular patterns of ADCC predominantly showed moderate immune-staining and solid patterns of ADCC exhibited predominantly mild immunostaining. Depending on the intensity of staining, we found a significant P value between PA and the solid variant of ADCC.Conclusion:E-cadherin proved to be a better marker for epithelial phenotypes in PAs. In ADCC difference in staining intensity between different histological subtypes suggests that further studies should be done to assess the usefulness of an immuno-marker to know the aggressive behavior of ADCC.

Expression of E-cadherin and Ki-67 in canine apocrine and sebaceous gland tumors

A histopathological analysis of 13 apocrine gland tumors and 22 sebaceous gland tumors in dogs was performed, which were submitted to the Department of Veterinary Pathology from 1 January 2010 to 31 December 2012. An association between the immunohistochemical expression of E-cadherin and Ki-67, the type, and the biological behavior of the tumors was investigated. The mean age of dogs with apocrine gland tumors was 10.15 years, and 10.91 years for sebaceous gland tumors. According to the histopathological analysis, 53.8% carcinomas and 46.2% adenomas of the apocrine glands were found. In the sebaceous gland tumors, epitheliomas were dominant (50.0%), followed by adenomas (36.4%) and carcinomas (13.6%). Survival time in dogs with sebaceous gland tumors was shortest in patients with diagnosed epitheliomas, intermediate in adenomas, and longest in carcinomas. The incidence of metastases was low in the groups with sebaceous adenomas and epitheliomas, and recurrence was most common in carcinomas. The average survival time was about the same for apocrine gland adenomas and carcinomas, with a higher incidence of metastases and recurrence for carcinomas. Immunohistochemical analysis of E-cadherin and Ki-67 expression confirmed the efficiency of this method for the accurate histological classification of apocrine and especially sebaceous gland tumors. The analyzes performed showed that the location and intensity of E-cadherin expression can be helpful in predicting the biological behavior of sebaceous gland tumors. In contrast to sebaceous gland tumors, the analysis of apocrine gland tumors showed that there was no correlation between E-cadherin expression and the biological behavior of apocrine gland tumors.

Association of E-cadherin & vimentin expression with clinicopathological parameters in lingual squamous cell carcinomas & their role in incomplete epithelial mesenchymal transition.

Background & objectives:Lingual squamous cell carcinomas (SCC) pose a major public health burden in India. Epithelial-mesenchymal transition (EMT) is the conversion of an epithelial cell to a mesenchymal phenotype at the invasive front (IF) enhancing invasiveness of these cells which may be studied using immunohistochemistry. The objective of this study was to assess the expression of E-cadherin and vimentin at the IF, and their correlation with the histological risk assessment score, clinicopathological parameters and lymph node metastasis.Methods:Thirty consecutive untreated patients diagnosed as lingual SCC who underwent hemiglossectomy over one year formed the study group. The immunohistochemical expression of E-cadherin and vimentin in the periphery as well as the centre of tumour islands was correlated with clinicopathological parameters, Brandwein-Gensler risk assessment score and lymph node metastasis, along with a correlation between the coexpression of two markers at the IF.Results:Loss of E-cadherin expression was seen at IF in 83.3 per cent (25/30) cases. Out of these, 20 per cent (5/25) showed a corresponding gain in vimentin expression (complete epithelial-mesenchymal transition) and 80 per cent (20/25) did not. Overall, 16.6 per cent (5/30) cases showed complete EMT. However, no correlation between E- cadherin and vimentin expression at the IF was found. No statistical significance was found between E-cadherin loss and vimentin gain at the IF, with the various parameters or the risk score.Interpretation & conclusions:The present study suggests that the cells at IF may metastasize even without a gain in vimentin expression (without classical EMT), as cohesive clusters showing incomplete EMT (E-cadh-/Vim-).

Immunohistochemical Expression of E-cadherin in Invasive Duct Carcinoma of the Breast

Breast cancer is the most frequent malignant tumor in women worldwide. E-Cadherin is a calcium-dependent epithelial cell adhesion molecule that is expressed at adherens junctions. Its loss may result in a poorly differentiated tumorphenotype. Cells with mutated E-cadherin demonstrate increased motility, altered organization of their actin cytoskeletonand associated with metastases, thereby providing evidence for its role as an invasion suppressor.Objectives: Assess the prognostic value of E-cadherin expression in invasive duct carcinoma (IDC) of the breastand to evaluate its correlations with other prognostic parameters.Materials and Methods: Immunohistochemical (IHC) E-Cadherin expression was studied in 60 IDC and the results were correlated with the clinico-pathological parameters.Results: E-cadherin was positive in 46/60 (76.7%) of IDC; 15/60 (25%) showed strong membranous staining. A significant inverse correlation was found between E-cadherin expression and number of lymph node metastasis.Conclusions: Loss of E-cadherin expression is a predictive of invasion and metastasis in IDC.

Utility of immunohistochemical expression of E-cadherin in colorectal carcinoma.

IntroductionReduced expression of E-cadherin, an intercellular junction protein, is associated with differentiation and metastasis of multiple cancers, including colorectal cancer.AimTo investigate the utility of the immunohistochemistry of E-cadherin as a prognostic marker for colorectal cancer (CRC).Material and methodsImmunohistochemical analysis for E-cadherin was performed on 100 paraffin blocks retrieved from resected specimens of CRC patients. The collected data were statistically analysed.ResultsAmong the 100 patients, men comprised 58% and the majority had tumour size of 5–10 cm (55%). Grade II CRC was more common (74%) than grade I and III (13% each). The correlation of E-cadherin expression with lymph node involvement was statistically significant, as revealed by p-value < 0.01, with about 27% in N1 and 13% in N2 stage. E-cadherin expression was significantly correlated with tumour differentiation pattern (p < 0.01), wherein out of 13 poorly differentiated carcinomas, 38.5% and 30.5% of samples showed negative and weak E-cadherin staining, respectively.ConclusionsFurthermore, a shift from membranous E-cadherin staining in normal cells to cytoplasmic and mixed staining was observed in cancer cells. The study indicates that immunohistochemical E-cadherin expression has prognostic value, as revealed by its loss of expression in poorly differentiated cells and lymph node metastasis.

Immunohistochemical E-cadherin Expression and Response to Chemotherapy in Breast Cancer Subjects

Cell-cell adhesion, which is essential for the maintenance of epithelial plasticity, is mediated by a class of proteins called cadherins. Immunohistochemical study of E-cadherin has been reported to be important in predicting response to chemotherapy in breast cancer subjects. An inverse relationship has been documented between expression of E-cadherin and invasiveness of tumors. The aim of the study is to assess E-cadherin expression in patients of carcinoma breast and to study correlation of E-cadherin expression with clinical response to neoadjuvant chemotherapy. A prospective single-center study was conducted with a sample size of 33 patients of carcinoma breast who were planned for neoadjuvant chemotherapy. E-cadherin expression in core needle biopsy specimens was assessed using immunohistochemistry. The response of the patients to chemotherapy was assessed clinically and pathologically. E-cadherin expression has no association with tumor size and grade, lymph node status, and estrogen receptor, progesterone receptor, or Her-2/neu receptor positivity. Stronger expression of E-cadherin intensity and proportion was associated with good clinical response as well as higher pathological complete response after neoadjuvant chemotherapy. E-cadherin expression correlates with response to neoadjuvant chemotherapy in patients of carcinoma breast, indicating its role as prognostic marker in predicting response to neoadjuvant chemotherapy.