Immunohistochemical Expression of E-Cadherin and β-Catenin in Oral Squamous Cell Carcinoma.

Abstract

The E-cadherin/β-catenin protein complexes are actively involved in the epithelial-to-mesenchymal transition. Alterations in cadherin or catenin expression or function, play important roles in the development of invasive or metastatic phenotypes of cancers. The aim of this study was to assess the expression of E-cadherin and β-catenin in oral squamous cell carcinoma (OSCC) patients and to compare this with their clinico-pathological parameters. This was a cross-sectional study to assess the immunohistochemical expression of E-cadherin and β-catenin in 41 cases of OSCC. Data were analyzed using version 26 of SPSS software. Qualitative data were compared using chi-square statistics. Quantitative data were summarized using mean, standard deviation, and confidence interval and compared using a one-way analysis of variance test. The level of significance was set at P < 0.05. Overall, 95.1% of the cases had positive membrane expression for E-cadherin, while cytoplasmic staining was seen in 90.2% cases. Positive nuclear staining was seen in 46.3% cases. There was a decrease in the percentage of cytoplasmic and nuclear expression of E-cadherin as the OSCC became more poorly differentiated (χ2 = 13.96, P = 0.016). Also, a decrease in the percentage of nuclear expression of β-catenin in poorly differentiated cases was seen. However, no statistically significant difference was seen in the expression of β-catenin between the different histologic grades (χ2 = 4.8, P = 0.4). This study shows a reduction in the expression of E-cadherin and β-catenin as OSCC becomes less differentiated.